Restriction map for pF1KA1404
##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AhdI        ApaI        AscI        Asp718I     AspEI       
AspI        BamHI       BbeI        BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      Bst1107I    BstUI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      FseI        
HpaI        KasI        KpnI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PflFI       PmeI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1279  GTCTAC               GT^[AC][GT]AC
AcyI         3532  GGCGTC               G[GA]^CG[CT]C
Aor51HI      5231  AGCGCT               AGC^GCT
AseI         1744  ATTAAT               AT^TAAT
AsnI         1744  ATTAAT               AT^TAAT
AvaI         3665  CCCGAG               C^[CT]CG[GA]G
AviII        3500  TGCGCA               TGC^GCA
AvrII         160  CCTAGG               C^CTAGG
BbrPI        5601  CACGTG               CAC^GTG
BclI          683  TGATCA               T^GATCA
BglII        1425  AGATCT               A^GATCT
BlnI          160  CCTAGG               C^CTAGG
BlpI         4955  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4955  GCTCAGC              GC^T[ACGT]AGC
BsaAI        5601  CACGTG               [CT]AC^GT[GA]
BsaHI        3532  GGCGTC               G[GA]^CG[CT]C
BsmI         2334  GCATTC               GAATGC(1/-1)
BsoBI        3665  CCCGAG               C^[CT]CG[GA]G
BspLU11I     5392  ACATGT               A^CATGT
BssSI        5525  CTCGTG               CACGAG(-5/-1)
BstAPI       3062  GCAAAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4357  TTCGAA               TT^CGAA
CelII        4955  GCTCAGC              GC^T[ACGT]AGC
Eco47III     5231  AGCGCT               AGC^GCT
EcoRV        3636  GATATC               GAT^ATC
FbaI          683  TGATCA               T^GATCA
FspI         3500  TGCGCA               TGC^GCA
HgaI         3533  GCGTC                GACGC(5/10)
Hin1I        3532  GGCGTC               G[GA]^CG[CT]C
MfeI         2010  CAATTG               C^AATTG
MunI         2010  CAATTG               C^AATTG
NheI         1479  GCTAGC               G^CTAGC
NspV         4357  TTCGAA               TT^CGAA
PmaCI        5601  CACGTG               CAC^GTG
PmlI         5601  CACGTG               CAC^GTG
PshAI        5092  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1744  ATTAAT               AT^TAAT
ScaI         3425  AGTACT               AGT^ACT
SfuI         4357  TTCGAA               TT^CGAA
SgrAI        3991  CACCGGTG             C[GA]^CCGG[CT]G
SspI         4716  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflII        3292  CTTAAG               C^TTAAG
             4990  CTTAAG              
AgeI         3992  ACCGGT               A^CCGGT
             4236  ACCGGT              
BbvCI        2847  CCTCAGC              CCTCAGC(-5/-2)
             2088  GCTGAGG             
BcgI         1086  CGACAGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4597  CGACCCCCATGC        
BcnI         2505  CCGGG                CC^[GC]GG
             3482  CCGGG               
BfrI         3292  CTTAAG               C^TTAAG
             4990  CTTAAG              
BglI         2535  GCCCCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5546  GCCGCAATGGC         
BsaI          278  GAGACC               GGTCTC(1/5)
             4654  GGTCTC              
BsaWI        3992  ACCGGT               [AT]^CCGG[AT]
             4236  ACCGGT              
BsrDI        3048  CATTGC               GCAATG(2/0)
             5549  GCAATG              
BsrFI        3992  ACCGGT               [GA]^CCGG[CT]
             4236  ACCGGT              
BstEII       4203  GGTCACC              G^GT[ACGT]ACC
             4707  GGTCACC             
BstPI        4203  GGTCACC              G^GT[ACGT]ACC
             4707  GGTCACC             
Cfr10I       3992  ACCGGT               [GA]^CCGG[CT]
             4236  ACCGGT              
EcoO65I      4203  GGTCACC              G^GT[ACGT]ACC
             4707  GGTCACC             
HincII        853  GTTGAC               GT[CT]^[GA]AC
             4232  GTCAAC              
HindII        853  GTTGAC               GT[CT]^[GA]AC
             4232  GTCAAC              
NciI         2505  CCGGG                CC^[GC]GG
             3482  CCGGG               
PinAI        3992  ACCGGT               A^CCGGT
             4236  ACCGGT              
SacI          514  GAGCTC               GAGCT^C
              553  GAGCTC              
SexAI         307  ACCAGGT              A^CC[AT]GGT
             5294  ACCTGGT             
SphI          258  GCATGC               GCATG^C
             5691  GCATGC              
StuI         1538  AGGCCT               AGG^CCT
             4260  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        Asp700I     AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvI        BciVI       
BfaI        BmyI        BpmI        BpuAI       BsaBI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI        
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