Restriction map for pF1KA1402
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# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccII       AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BbeI        BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsiEI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MseI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tru9I       
Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI         1582  GTCTAC               GT^[AC][GT]AC
AccIII       1019  TCCGGA               T^CCGGA
AcyI          802  GGCGTC               G[GA]^CG[CT]C
Alw44I        913  GTGCAC               G^TGCAC
AlwNI        1279  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         913  GTGCAC               G^TGCAC
AspI         1732  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          98  GGATCC               G^GATCC
BbsI          196  GAAGAC               GAAGAC(2/6)
BlpI         1059  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1059  GCTGAGC              GC^T[ACGT]AGC
BpuAI         196  GAAGAC               GAAGAC(2/6)
BsaHI         802  GGCGTC               G[GA]^CG[CT]C
BseAI        1019  TCCGGA               T^CCGGA
BsiWI         364  CGTACG               C^GTACG
BsmI         1827  GAATGC               GAATGC(1/-1)
BspEI        1019  TCCGGA               T^CCGGA
BsrDI        1562  CATTGC               GCAATG(2/0)
BstXI        1535  CCAATGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1059  GCTGAGC              GC^T[ACGT]AGC
EcoNI         221  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1559  ATGCAT               ATGCA^T
Hin1I         802  GGCGTC               G[GA]^CG[CT]C
MroI         1019  TCCGGA               T^CCGGA
NdeI          554  CATATG               CA^TATG
NheI         1192  GCTAGC               G^CTAGC
NsiI         1559  ATGCAT               ATGCA^T
NspI         1557  ACATGC               [GA]CATG^[CT]
PaeR7I       1519  CTCGAG               C^TCGAG
PflFI        1732  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SgrAI        1372  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         1519  CTCGAG               C^T[CT][GA]AG
Tth111I      1732  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          341  CCACTTTGGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1519  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I        931  GGTACC               G^GTACC
             1664  GGTACC              
Aor51HI        36  AGCGCT               AGC^GCT
              964  AGCGCT              
Asp718I       931  GGTACC               G^GTACC
             1664  GGTACC              
BalI          588  TGGCCA               TGG^CCA
              854  TGGCCA              
BbvCI         594  CCTCAGC              CCTCAGC(-5/-2)
             2238  CCTCAGC             
BsaI          484  GAGACC               GGTCTC(1/5)
             1737  GGTCTC              
BsaWI        1019  TCCGGA               [AT]^CCGG[AT]
             2126  TCCGGT              
BssSI        1550  CACGAG               CACGAG(-5/-1)
             1592  CACGAG              
EarI         1765  CTCTTC               CTCTTC(1/4)
             2080  GAAGAG              
Eco47III       36  AGCGCT               AGC^GCT
              964  AGCGCT              
HgaI          779  GACGC                GACGC(5/10)
              803  GCGTC               
KpnI          931  GGTACC               GGTAC^C
             1664  GGTACC              
Ksp632I      1765  CTCTTC               CTCTTC(1/4)
             2080  GAAGAG              
MaeII        1205  ACGT                 A^CGT
             1733  ACGT                
MluNI         588  TGGCCA               TGG^CCA
              854  TGGCCA              
MscI          588  TGGCCA               TGG^CCA
              854  TGGCCA              
PflMI        1621  CCATTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1778  CCACCGTGTGG         
PpuMI         230  GGGACCC              [GA]G^G[AT]CC[CT]
              866  GGGACCC             
PvuII        1468  CAGCTG               CAG^CTG
             2272  CAGCTG              
TfiI          159  GAATC                G^A[AT]TC
              166  GATTC               
Van91I       1621  CCATTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1778  CCACCGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcgI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StuI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       XmaCI       XmaI        
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