Restriction map for pF1KA1333
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        Asp718I     
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     FbaI        FseI        FspI        
HaeIII      HapII       Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MluI        MluNI       MroI        MscI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI          544  GCGG                 CCGC(-3/-1)
AflIII        900  ACATGT               A^C[GA][CT]GT
AhdI         1525  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1681  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       767  AGCGCT               AGC^GCT
AseI         1047  ATTAAT               AT^TAAT
AsnI         1047  ATTAAT               AT^TAAT
Asp700I      1888  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1525  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         985  GGATCC               G^GATCC
BbsI         1888  GAAGAC               GAAGAC(2/6)
BfaI         2083  CTAG                 C^TAG
BglII         999  AGATCT               A^GATCT
BpmI         1928  CTCCAG               CTGGAG(16/14)
BpuAI        1888  GAAGAC               GAAGAC(2/6)
BsaBI        1488  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         810  CTCCTC               GAGGAG(10/8)
BsmFI         374  GGGAC                GGGAC(10/14)
BspLU11I      900  ACATGT               A^CATGT
BstBI         704  TTCGAA               TT^CGAA
CfoI          768  GCGC                 GCG^C
DraI         1939  TTTAAA               TTT^AAA
DraII        1282  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     1525  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          949  GAAGAG               CTCTTC(1/4)
Eco47III      767  AGCGCT               AGC^GCT
EcoO109I     1282  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         617  GAATTC               G^AATTC
EcoRV         202  GATATC               GAT^ATC
EcoT14I      1695  CCTTGG               C^C[AT][AT]GG
EcoT22I      1130  ATGCAT               ATGCA^T
FauI          544  GCGGG                CCCGC(4/6)
HaeII         767  AGCGCT               [GA]GCGC^[CT]
HgaI          361  GCGTC                GACGC(5/10)
HhaI          768  GCGC                 GCG^C
HinP1I        768  GCGC                 G^CGC
HindIII      1823  AAGCTT               A^AGCTT
Ksp632I       949  GAAGAG               CTCTTC(1/4)
MaeI         2083  CTAG                 C^TAG
MamI         1488  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          284  CACCCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1931  CAGCTG               C[AC]G^C[GT]G
NsiI         1130  ATGCAT               ATGCA^T
NspV          704  TTCGAA               TT^CGAA
PflMI        1106  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1282  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1047  ATTAAT               AT^TAAT
PstI          679  CTGCAG               CTGCA^G
PvuII        1931  CAGCTG               CAG^CTG
SfuI          704  TTCGAA               TT^CGAA
StyI         1695  CCTTGG               C^C[AT][AT]GG
Van91I       1106  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1888  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BbvI          681  GCAGC                GCAGC(8/12)
             1016  GCAGC               
BsaJI         644  CCTGGG               C^C[ACGT][ACGT]GG
             1695  CCTTGG              
BsmI         1546  GAATGC               GAATGC(1/-1)
             1132  GCATTC              
Bsp1407I      117  TGTACA               T^GTACA
              565  TGTACA              
BsrGI         117  TGTACA               T^GTACA
              565  TGTACA              
BstAPI        431  GCACCATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              535  GCAATAAATGC         
BstXI         342  CCAGTTTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              434  CCATTTGCTTGG        
BstYI         985  GGATCC               [GA]^GATC[CT]
              999  AGATCT              
Fnu4HI        681  GCAGC                GC^[ACGT]GC
             1016  GCAGC               
ItaI          681  GCAGC                GC^[ACGT]GC
             1016  GCAGC               
MflI          985  GGATCC               [GA]^GATC[CT]
              999  AGATCT              
NdeI         1160  CATATG               CA^TATG
             1178  CATATG              
NspI          900  ACATGT               [GA]CATG^[CT]
             1968  GCATGT              
SexAI          15  ACCTGGT              A^CC[AT]GGT
             1389  ACCTGGT             
SfcI          679  CTGCAG               C^T[GA][CT]AG
             1517  CTGTAG              
SspBI         117  TGTACA               T^GTACA
              565  TGTACA              
TseI          681  GCAGC                G^C[AT]GC
             1016  GCAGC               
XhoII         985  GGATCC               [GA]^GATC[CT]
              999  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      FokI        HinfI       
HphI        MaeIII      MboI        MboII       MfeI        
MnlI        MseI        MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     
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