Restriction map for pF1KA1331
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        Asp718I     
AspEI       AspHI       AspI        AviII       BalI        
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BsaAI       BsaI        BsgI        BsiHKAI     BsiWI       
BsmBI       BsmI        BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BssHII      Bst1107I    BstUI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MfeI        MluI        
MluNI       MscI        MslI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PflFI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     XbaI        


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# ONE CUT ENZYMES (   66 enzymes) #
###################################

AccIII       1724  TCCGGA               T^CCGGA
AcyI          593  GGCGTC               G[GA]^CG[CT]C
AgeI           50  ACCGGT               A^CCGGT
AocI          538  CCTCAGG              CC^T[ACGT]AGG
ApaI           71  GGGCCC               GGGCC^C
AseI         1246  ATTAAT               AT^TAAT
AsnI         1246  ATTAAT               AT^TAAT
AvrII        1137  CCTAGG               C^CTAGG
BamHI         331  GGATCC               G^GATCC
BanII          71  GGGCCC               G[GA]GC[CT]^C
BbsI          479  GAAGAC               GAAGAC(2/6)
BbvI          196  GCTGC                GCAGC(8/12)
BciVI         940  GGATAC               GTATCC(6/5)
BlnI         1137  CCTAGG               C^CTAGG
BpmI          949  CTGGAG               CTGGAG(16/14)
BpuAI         479  GAAGAC               GAAGAC(2/6)
BsaBI        1567  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         593  GGCGTC               G[GA]^CG[CT]C
BseAI        1724  TCCGGA               T^CCGGA
Bsp1407I        2  TGTACA               T^GTACA
BspEI        1724  TCCGGA               T^CCGGA
BsrDI        1004  GCAATG               GCAATG(2/0)
BsrFI          50  ACCGGT               [GA]^CCGG[CT]
BsrGI           2  TGTACA               T^GTACA
BssSI        1609  CACGAG               CACGAG(-5/-1)
BstAPI       1707  GCACCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         297  TTCGAA               TT^CGAA
BstEII       1348  GGTTACC              G^GT[ACGT]ACC
BstPI        1348  GGTTACC              G^GT[ACGT]ACC
Bsu36I        538  CCTCAGG              CC^T[ACGT]AGG
Cac8I         199  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI           90  GCGC                 GCG^C
Cfr10I         50  ACCGGT               [GA]^CCGG[CT]
DraIII        169  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          489  TGGCCG               [CT]^GGCC[GA]
Eco81I        538  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1348  GGTTACC              G^GT[ACGT]ACC
HaeII          89  GGCGCT               [GA]GCGC^[CT]
HgaI          594  GCGTC                GACGC(5/10)
HhaI           90  GCGC                 GCG^C
Hin1I         593  GGCGTC               G[GA]^CG[CT]C
HinP1I         90  GCGC                 G^CGC
MamI         1567  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1724  TCCGGA               T^CCGGA
NspI          344  ACATGC               [GA]CATG^[CT]
NspV          297  TTCGAA               TT^CGAA
PaeR7I       1193  CTCGAG               C^TCGAG
PflMI         755  CCAAAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          50  ACCGGT               A^CCGGT
PpuMI         837  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1246  ATTAAT               AT^TAAT
SapI          769  GAAGAGC              GCTCTTC(1/4)
ScaI         1315  AGTACT               AGT^ACT
SexAI         483  ACCAGGT              A^CC[AT]GGT
SfaNI        1713  GATGC                GCATC(5/9)
SfcI          113  CTACAG               C^T[GA][CT]AG
SfuI          297  TTCGAA               TT^CGAA
SmaI           68  CCCGGG               CCC^GGG
SmlI         1193  CTCGAG               C^T[CT][GA]AG
SspBI           2  TGTACA               T^GTACA
TseI          196  GCTGC                G^C[AT]GC
Van91I        755  CCAAAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          230  CCACAAAGCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1193  CTCGAG               C^TCGAG
XmaCI          68  CCCGGG               C^CCGGG
XmaI           68  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          987  CCGC                 CCGC(-3/-1)
               87  GCGG                
Asp700I      1399  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1744  GAAATCCTTC          
BcnI           68  CCCGG                CC^[GC]GG
               69  CCGGG               
BfaI         1138  CTAG                 C^TAG
             1679  CTAG                
BmyI           58  GTGCCC               G[AGT]GC[ACT]^C
               71  GGGCCC              
BsaWI          50  ACCGGT               [AT]^CCGG[AT]
             1724  TCCGGA              
BseRI         613  GAGGAG               GAGGAG(10/8)
             1196  GAGGAG              
BsiEI          48  CGACCG               CG[GA][CT]^CG
               52  CGGTCG              
BsmAI        1039  GAGAC                GTCTC(1/5)
              185  GTCTC               
Bsp1286I       58  GTGCCC               G[AGT]GC[ACT]^C
               71  GGGCCC              
BstF5I       1155  GGATG                GGATG(2/0)
             1539  GGATG               
BstYI         331  GGATCC               [GA]^GATC[CT]
             1508  GGATCT              
DraII          71  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              837  GGGACCT             
DsaI          239  CCATGG               C^C[GA][CT]GG
             1143  CCATGG              
EcoO109I       71  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              837  GGGACCT             
Fnu4HI         87  GCGGC                GC^[ACGT]GC
              196  GCTGC               
FokI         1155  GGATG                GGATG(9/13)
             1539  GGATG               
HphI         1446  GGTGA                GGTGA(8/7)
              168  TCACC               
ItaI           87  GCGGC                GC^[ACGT]GC
              196  GCTGC               
MaeI         1138  CTAG                 C^TAG
             1679  CTAG                
MflI          331  GGATCC               [GA]^GATC[CT]
             1508  GGATCT              
MspA1I        223  CAGCTG               C[AC]G^C[GT]G
             1624  CAGCTG              
NciI           68  CCCGG                CC^[GC]GG
               69  CCGGG               
NcoI          239  CCATGG               C^CATGG
             1143  CCATGG              
PvuII         223  CAGCTG               CAG^CTG
             1624  CAGCTG              
TaqI          298  TCGA                 T^CGA
             1194  TCGA                
Tsp45I        383  GTGAC                ^GT[GC]AC
              403  GTGAC               
TthHB8I       298  TCGA                 T^CGA
             1194  TCGA                
XhoII         331  GGATCC               [GA]^GATC[CT]
             1508  GGATCT              
XmnI         1399  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1744  GAAATCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AfaI        AluI        AlwI        AlwNI       AvaI        
AvaII       BanI        BsaJI       BsiYI       BslI        
BsmFI       BsoBI       BsrI        BssKI       BstNI       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
EcoT14I     HaeIII      HapII       HinfI       HpaII       
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TfiI        Tru9I       
Tsp509I     TspRI       
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