Restriction map for pF1KA1321
 (Linear) (Six-base) MAPSORT of: KIAA1321.seq  Check: 530  from: 1  to: 2085



KIAA1321.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0     392    2085 
   Size:        392    1693
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     956    1364    2085 
   Size:        424     532     408     721

  Fragments arranged by size: 

                 721     532     424     408
 
AfeI AGC'GCT

Cuts at:      0    1214    2085 
   Size:       1214     871
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1656    1688    2085 
   Size:       1656      32     397

  Fragments arranged by size: 

                1656     397      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1267    1302    1387    1666    2085 
   Size:       1267      35      85     279     419

  Fragments arranged by size: 

                1267     419     279      85      35
 
ApoI r'AATT_y

Cuts at:      0     456    2019    2085 
   Size:        456    1563      66

  Fragments arranged by size: 

                1563     456      66
 
BanII G_rGCy'C

Cuts at:      0    1544    2085 
   Size:       1544     541
 
BbvCI CC'TCA_GC

Cuts at:      0      29      68    2085 
   Size:         29      39    2017

  Fragments arranged by size: 

                2017      39      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     860    1015    1538    1577    1778    2085 
   Size:        860     155     523      39     201     307

  Fragments arranged by size: 

                 860     523     307     201     155      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     323     536     878    1271    1345    1720    2085 
   Size:        226      97     213     342     393      74     375     365

  Fragments arranged by size: 

                 393     375     365     342     226     213      97      74
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      29      68    2085 
   Size:         29      39    2017

  Fragments arranged by size: 

                2017      39      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1703    2085 
   Size:       1703     382
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     736    2085 
   Size:        736    1349
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     224     254    2085 
   Size:        224      30    1831

  Fragments arranged by size: 

                1831     224      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19    2085 
   Size:         19    2066
 
BseYI C'CCAG_C

Cuts at:      0     203    1955    2085 
   Size:        203    1752     130

  Fragments arranged by size: 

                1752     203     130
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739    2085 
   Size:        739    1346
 
BsiHKAI G_wGCw'C

Cuts at:      0    1453    2085 
   Size:       1453     632
 
BsmI GAATG_Cn'

Cuts at:      0    1733    2085 
   Size:       1733     352
 
Bsp1286I G_dGCh'C

Cuts at:      0    1453    1544    2085 
   Size:       1453      91     541

  Fragments arranged by size: 

                1453     541      91
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179     888    1808    2085 
   Size:        179     709     920     277

  Fragments arranged by size: 

                 920     709     277     179
 
BsrBI CCG'CTC

Cuts at:      0     647    2085 
   Size:        647    1438
 
BssSI C'ACGA_G

Cuts at:      0     996    2085 
   Size:        996    1089
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1440    2085 
   Size:       1440     645
 
BstBI TT'CG_AA

Cuts at:      0     826    2085 
   Size:        826    1259
 
BstEII G'GTnAC_C

Cuts at:      0    1795    2085 
   Size:       1795     290
 
BstYI r'GATC_y

Cuts at:      0     637    1464    2034    2085 
   Size:        637     827     570      51

  Fragments arranged by size: 

                 827     637     570      51
 
Bsu36I CC'TnA_GG

Cuts at:      0    1659    1972    2085 
   Size:       1659     313     113

  Fragments arranged by size: 

                1659     313     113
 
BtgI C'CryG_G

Cuts at:      0    2066    2085 
   Size:       2066      19
 
BtsI GCAGTG_nn'

Cuts at:      0    1050    1300    2085 
   Size:       1050     250     785

  Fragments arranged by size: 

                1050     785     250
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     548     583    2085 
   Size:        548      35    1502

  Fragments arranged by size: 

                1502     548      35
 
DraI TTT'AAA

Cuts at:      0     323    1011    2085 
   Size:        323     688    1074

  Fragments arranged by size: 

                1074     688     323
 
DraIII CAC_nnn'GTG

Cuts at:      0    1642    2085 
   Size:       1642     443
 
EaeI y'GGCC_r

Cuts at:      0      15    2085 
   Size:         15    2070
 
EarI CTCTTCn'nnn_

Cuts at:      0    1122    1344    2085 
   Size:       1122     222     741

  Fragments arranged by size: 

                1122     741     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     323     424     536     878     956    1271    1345 
   Size:        226      97     101     112     342      78     315      74

Cuts at:   1345    1364    1720    2085 
   Size:         19     356     365

  Fragments arranged by size: 

                 365     356     342     315     226     112     101      97
                  78      74      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0     366    1200    2085 
   Size:        366     834     885

  Fragments arranged by size: 

                 885     834     366
 
EcoRV GAT'ATC

Cuts at:      0    1503    2085 
   Size:       1503     582
 
HaeII r_GCGC'y

Cuts at:      0    1216    2085 
   Size:       1216     869
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     511     543    1456    1488    1701    1733    1851    1883 
   Size:        511      32     913      32     213      32     118      32

Cuts at:   1883    2085 
   Size:        202

  Fragments arranged by size: 

                 913     511     213     202     118      32      32      32
                  32
 
MslI CAynn'nnrTG

Cuts at:      0    1793    2085 
   Size:       1793     292
 
MspA1I CmG'CkG

Cuts at:      0     216     976    1387    2085 
   Size:        216     760     411     698

  Fragments arranged by size: 

                 760     698     411     216
 
NcoI C'CATG_G

Cuts at:      0    2066    2085 
   Size:       2066      19
 
NspI r_CATG'y

Cuts at:      0     970    2085 
   Size:        970    1115
 
PfoI T'CCnGG_A

Cuts at:      0    1361    2085 
   Size:       1361     724
 
PpuMI rG'GwC_Cy

Cuts at:      0     366    1200    2085 
   Size:        366     834     885

  Fragments arranged by size: 

                 885     834     366
 
PshAI GACnn'nnGTC

Cuts at:      0     748    1370    2085 
   Size:        748     622     715

  Fragments arranged by size: 

                 748     715     622
 
PsiI TTA'TAA

Cuts at:      0    1534    2085 
   Size:       1534     551
 
PstI C_TGCA'G

Cuts at:      0     176    1805    2085 
   Size:        176    1629     280

  Fragments arranged by size: 

                1629     280     176
 
PvuII CAG'CTG

Cuts at:      0     976    1387    2085 
   Size:        976     411     698

  Fragments arranged by size: 

                 976     698     411
 
SexAI A'CCwGG_T

Cuts at:      0     921    2085 
   Size:        921    1164
 
SfcI C'TryA_G

Cuts at:      0     172     530     792    1801    2085 
   Size:        172     358     262    1009     284

  Fragments arranged by size: 

                1009     358     284     262     172
 
SmlI C'TyrA_G

Cuts at:      0    1718    2085 
   Size:       1718     367
 
SpeI A'CTAG_T

Cuts at:      0    1711    1753    2085 
   Size:       1711      42     332

  Fragments arranged by size: 

                1711     332      42
 
SphI G_CATG'C

Cuts at:      0     970    2085 
   Size:        970    1115
 
StuI AGG'CCT

Cuts at:      0    1937    2085 
   Size:       1937     148
 
StyI C'CwwG_G

Cuts at:      0     197     692    1685    1775    2066    2085 
   Size:        197     495     993      90     291      19

  Fragments arranged by size: 

                 993     495     291     197      90      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     877    2085 
   Size:        877    1208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1740    1772    2085 
   Size:       1740      32     313

  Fragments arranged by size: 

                1740     313      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1652    2085 
   Size:       1652     433
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AloI    AlwNI     ApoI    BanII    BbvCI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I
     MslI   MspA1I     NcoI     NspI     PfoI    PpuMI    PshAI     PsiI
     PstI    PvuII    SexAI     SfcI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BstXI  BstZ17I
    BtgZI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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