Restriction map for pF1KA1282
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        BamHI       BbrPI       
BclI        BfrI        BsaAI       BsaBI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HpaI        KpnI        MamI        
MfeI        MluI        MseI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          127  GTCTAC               GT^[AC][GT]AC
AccIII        629  TCCGGA               T^CCGGA
AcsI          834  GAATTT               [GA]^AATT[CT]
AgeI         1049  ACCGGT               A^CCGGT
AocI         3011  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       939  AGCGCT               AGC^GCT
ApoI          834  GAATTT               [GA]^AATT[CT]
AviII        1343  TGCGCA               TGC^GCA
AvrII        1914  CCTAGG               C^CTAGG
BalI         1143  TGGCCA               TGG^CCA
BciVI        1586  GTATCC               GTATCC(6/5)
BlnI         1914  CCTAGG               C^CTAGG
BseAI         629  TCCGGA               T^CCGGA
BsmBI        2942  CGTCTC               CGTCTC(1/5)
BspEI         629  TCCGGA               T^CCGGA
BssHII       1695  GCGCGC               G^CGCGC
BstXI        3201  CCATGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3011  CCTCAGG              CC^T[ACGT]AGG
DraIII       1477  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2655  GACAGAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      939  AGCGCT               AGC^GCT
Eco81I       3011  CCTCAGG              CC^T[ACGT]AGG
FspI         1343  TGCGCA               TGC^GCA
HincII        973  GTCAAC               GT[CT]^[GA]AC
HindII        973  GTCAAC               GT[CT]^[GA]AC
HindIII      2638  AAGCTT               A^AGCTT
MluNI        1143  TGGCCA               TGG^CCA
MroI          629  TCCGGA               T^CCGGA
MscI         1143  TGGCCA               TGG^CCA
PaeR7I       2963  CTCGAG               C^TCGAG
PinAI        1049  ACCGGT               A^CCGGT
PshAI        2693  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1845  CCTGCAGG             CCTGCA^GG
ScaI         1619  AGTACT               AGT^ACT
SfiI         2530  GGCCAGTCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          167  CCCGGG               CCC^GGG
SphI         1610  GCATGC               GCATG^C
Sse8387I     1845  CCTGCAGG             CCTGCA^GG
TfiI         1168  GAATC                G^A[AT]TC
Tsp509I       835  AATT                 ^AATT
XcmI          701  CCACACTCTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2963  CTCGAG               C^TCGAG
XmaCI         167  CCCGGG               C^CCGGG
XmaI          167  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII        977  ACGTGT               A^C[GA][CT]GT
             1409  ACGTGT              
Asp700I        33  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1434  GAAGATCTTC          
BbsI         1002  GAAGAC               GAAGAC(2/6)
             2485  GAAGAC              
BglII        1436  AGATCT               A^GATCT
             2454  AGATCT              
BlpI          283  GCTGAGC              GC^T[ACGT]AGC
             2097  GCTCAGC             
Bpu1102I      283  GCTGAGC              GC^T[ACGT]AGC
             2097  GCTCAGC             
BpuAI        1002  GAAGAC               GAAGAC(2/6)
             2485  GAAGAC              
BsaI         3017  GAGACC               GGTCTC(1/5)
             2220  GGTCTC              
CelII         283  GCTGAGC              GC^T[ACGT]AGC
             2097  GCTCAGC             
NaeI            3  GCCGGC               GCC^GGC
              452  GCCGGC              
NgoMIV          3  GCCGGC               G^CCGGC
              452  GCCGGC              
PflMI        2432  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3171  CCACAGCCTGG         
PleI         1221  GACTC                GAGTC(4/5)
             2995  GACTC               
SapI          303  GAAGAGC              GCTCTTC(1/4)
              114  GCTCTTC             
SexAI         913  ACCTGGT              A^CC[AT]GGT
             1232  ACCTGGT             
Van91I       2432  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3171  CCACAGCCTGG         
XmnI           33  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1434  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbvCI       
BbvI        BcgI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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