Restriction map for
pF1KA1265
(Circular) (Six-base) MAPSORT of: pF1KA1265.seq Check: 7316 from: 1 to: 5606
pF1KA1265 5606 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2306 2644 2306
Size: 338 5268
Acc65I G'GTAC_C
Cuts at: 2622 2622
Size: 5606
AclI AA'CG_TT
Cuts at: 5446 5446
Size: 5606
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1316 1847 2136 2500 3431 3863 4610 4878
Size: 1089 531 289 364 931 432 747 268
Cuts at: 4878 227
Size: 955
Fragments arranged by size:
1089 955 931 747 531 432 364 289
268
AfeI AGC'GCT
Cuts at: 212 212
Size: 5606
AflIII A'CryG_T
Cuts at: 1050 2958 4062 5039 1050
Size: 1908 1104 977 1617
Fragments arranged by size:
1908 1617 1104 977
AgeI A'CCGG_T
Cuts at: 3994 3994
Size: 5606
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1642 1642
Size: 5606
AlwNI CAG_nnn'CTG
Cuts at: 1774 2407 2911 4478 1774
Size: 633 504 1567 2902
Fragments arranged by size:
2902 1567 633 504
ApaLI G'TGCA_C
Cuts at: 4376 4376
Size: 5606
ApoI r'AATT_y
Cuts at: 130 256 513 834 1417 2520 2610 4959 5215
Size: 126 257 321 583 1103 90 2349 256
Cuts at: 5215 130
Size: 521
Fragments arranged by size:
2349 1103 583 521 321 257 256 126
90
AseI AT'TA_AT
Cuts at: 20 404 20
Size: 384 5222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5606
AvaI C'yCGr_G
Cuts at: 2626 2626
Size: 5606
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 524 557 524
Size: 33 5573
BamHI G'GATC_C
Cuts at: 2080 2631 2080
Size: 551 5055
BanI G'GyrC_C
Cuts at: 1156 2036 2622 3036 3284 3319 1156
Size: 880 586 414 248 35 3443
Fragments arranged by size:
3443 880 586 414 248 35
BanII G_rGCy'C
Cuts at: 2165 2620 3650 2165
Size: 455 1030 4121
Fragments arranged by size:
4121 1030 455
BbeI G_GCGC'C
Cuts at: 3040 3288 3040
Size: 248 5358
BbsI GAAGACnn'nnnn_
Cuts at: 2217 2217
Size: 5606
BbvCI CC'TCA_GC
Cuts at: 2014 2014
Size: 5606
BciVI GTATCCnnnnn_n'
Cuts at: 552 3504 4265 5606 552
Size: 2952 761 1341 552
Fragments arranged by size:
2952 1341 761 552
BclI T'GATC_A
Cuts at: 672 873 1509 2126 3126 672
Size: 201 636 617 1000 3152
Fragments arranged by size:
3152 1000 636 617 201
BfrBI ATG'CAT
Cuts at: 621 621
Size: 5606
BglI GCCn_nnn'nGGC
Cuts at: 2329 2329
Size: 5606
BglII A'GATC_T
Cuts at: 993 1086 3121 5158 993
Size: 93 2035 2037 1441
Fragments arranged by size:
2037 2035 1441 93
BlpI GC'TnA_GC
Cuts at: 2718 2718
Size: 5606
Bme1580I G_kGCm'C
Cuts at: 3231 3324 4380 3231
Size: 93 1056 4457
Fragments arranged by size:
4457 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 710 2818 2899 3234 5378 710
Size: 2108 81 335 2144 938
Fragments arranged by size:
2144 2108 938 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3973 3973
Size: 5606
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 5606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2782 3661 4153 4451 1774
Size: 1008 879 492 298 2929
Fragments arranged by size:
2929 1008 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 748 5310 748
Size: 4562 1044
BsaAI yAC'GTr
Cuts at: 2959 2959
Size: 5606
BsaBI GATnn'nnATC
Cuts at: 6 998 1103 2125 3120 5157 6
Size: 992 105 1022 995 2037 455
Fragments arranged by size:
2037 1022 995 992 455 105
BsaHI Gr'CG_yC
Cuts at: 1469 3037 3285 3987 5483 1469
Size: 1568 248 702 1496 1592
Fragments arranged by size:
1592 1568 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2795 3316 3994 4268 4415 4826 2795
Size: 521 678 274 147 411 3575
Fragments arranged by size:
3575 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 810 840 810
Size: 30 5576
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1924 2305 1924
Size: 381 5225
BseYI C'CCAG_C
Cuts at: 3031 3789 4366 4862 3031
Size: 758 577 496 3775
Fragments arranged by size:
3775 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2195 1964
Size: 231 5375
BsiEI CG_ry'CG
Cuts at: 104 3148 3194 4402 104
Size: 3044 46 1208 1308
Fragments arranged by size:
3044 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2620 3398 3588 4380 2620
Size: 778 190 792 3846
Fragments arranged by size:
3846 792 778 190
BsmI GAATG_Cn'
Cuts at: 495 2046 495
Size: 1551 4055
BsmBI CGTCTCn'nnnn_
Cuts at: 1460 1460
Size: 5606
Bsp1286I G_dGCh'C
Cuts at: 2165 2620 3231 3324 3398 3588 3650 4380 2165
Size: 455 611 93 74 190 62 730 3391
Fragments arranged by size:
3391 730 611 455 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4826 4826
Size: 5606
BspHI T'CATG_A
Cuts at: 408 969 1110 1956 2232 4782 408
Size: 561 141 846 276 2550 1232
Fragments arranged by size:
2550 1232 846 561 276 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2656 2870 3172 3553 2656
Size: 214 302 381 4709
Fragments arranged by size:
4709 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3898 3952 5436 5601 50
Size: 3848 54 1484 165 55
Fragments arranged by size:
3848 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 168 177 744 3518 168
Size: 9 567 2774 2256
Fragments arranged by size:
2774 2256 567 9
BsrFI r'CCGG_y
Cuts at: 3604 3785 3994 3604
Size: 181 209 5216
Fragments arranged by size:
5216 209 181
BsrGI T'GTAC_A
Cuts at: 921 945 921
Size: 24 5582
BssSI C'ACGA_G
Cuts at: 3877 4235 3877
Size: 358 5248
BstAPI GCAn_nnn'nTGC
Cuts at: 179 2173 2407 2983 179
Size: 1994 234 576 2802
Fragments arranged by size:
2802 1994 576 234
BstBI TT'CG_AA
Cuts at: 3967 3967
Size: 5606
BstXI CCAn_nnnn'nTGG
Cuts at: 2553 2553
Size: 5606
BstYI r'GATC_y
Cuts at: 1 414 993 1086 2080 2393 2631 3098 3121
Size: 413 579 93 994 313 238 467 23
Cuts at: 3121 3456 3702 4714 4800 4812 5158 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 994 579 467 449 413 346 335
313 246 238 93 86 23 12
BstZ17I GTA'TAC
Cuts at: 2307 2307
Size: 5606
BtgI C'CryG_G
Cuts at: 198 1992 2061 198
Size: 1794 69 3743
Fragments arranged by size:
3743 1794 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3556 3737 3900 3556
Size: 181 163 5262
Fragments arranged by size:
5262 181 163
BtsI GCAGTG_nn'
Cuts at: 1644 2405 2849 1644
Size: 761 444 4401
Fragments arranged by size:
4401 761 444
DraI TTT'AAA
Cuts at: 512 2605 512
Size: 2093 3513
DrdI GACnn_nn'nnGTC
Cuts at: 3312 4170 3312
Size: 858 4748
EaeI y'GGCC_r
Cuts at: 420 3191 3365 3756 3783 5128 420
Size: 2771 174 391 27 1345 898
Fragments arranged by size:
2771 1345 898 391 174 27
EagI C'GGCC_G
Cuts at: 3191 3191
Size: 5606
EarI CTCTTCn'nnn_
Cuts at: 1901 3629 3839 1901
Size: 1728 210 3668
Fragments arranged by size:
3668 1728 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3814 4124 4270 5416 3814
Size: 310 146 1146 4004
Fragments arranged by size:
4004 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2618 2618
Size: 5606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1316 1847 2136 2500 3431 3863 4610 4878
Size: 1089 531 289 364 931 432 747 268
Cuts at: 4878 227
Size: 955
Fragments arranged by size:
1089 955 931 747 531 432 364 289
268
EcoO109I rG'GnC_Cy
Cuts at: 2745 2745
Size: 5606
EcoRI G'AATT_C
Cuts at: 2610 2610
Size: 5606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1036 1852 3625 1036
Size: 816 1773 3017
Fragments arranged by size:
3017 1773 816
FspI TGC'GCA
Cuts at: 3387 3387
Size: 5606
HaeII r_GCGC'y
Cuts at: 214 3040 3288 4310 5289 214
Size: 2826 248 1022 979 531
Fragments arranged by size:
2826 1022 979 531 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1071 1103 3448 3478 3480 3510 1071
Size: 32 2345 30 2 30 3167
Fragments arranged by size:
3167 2345 32 30 30 2
HincII GTy'rAC
Cuts at: 2645 2645
Size: 5606
HindIII A'AGCT_T
Cuts at: 1738 1738
Size: 5606
KasI G'GCGC_C
Cuts at: 3036 3284 3036
Size: 248 5358
KpnI G_GTAC'C
Cuts at: 2626 2626
Size: 5606
MfeI C'AATT_G
Cuts at: 2168 2300 2168
Size: 132 5474
MluI A'CGCG_T
Cuts at: 5039 5039
Size: 5606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4277 4461 4277
Size: 184 5422
MscI TGG'CCA
Cuts at: 422 3367 422
Size: 2945 2661
MslI CAynn'nnrTG
Cuts at: 182 197 428 563 620 941 1109 1523 1529
Size: 15 231 135 57 321 168 414 6
Cuts at: 1529 1535 1547 1553 1654 1937 1961 2060 2341
Size: 6 12 6 101 283 24 99 281
Cuts at: 2341 2465 3595 3722 182
Size: 124 1130 127 2066
Fragments arranged by size:
2066 1130 414 321 283 281 231 168
135 127 124 101 99 57 24 15
12 6 6 6
MspA1I CmG'CkG
Cuts at: 2359 2717 3031 3391 4404 4649 2359
Size: 358 314 360 1013 245 3316
Fragments arranged by size:
3316 1013 360 358 314 245
NaeI GCC'GGC
Cuts at: 3787 3787
Size: 5606
NarI GG'CG_CC
Cuts at: 3037 3285 3037
Size: 248 5358
NcoI C'CATG_G
Cuts at: 198 2061 198
Size: 1863 3743
NdeI CA'TA_TG
Cuts at: 119 617 119
Size: 498 5108
NgoMIV G'CCGG_C
Cuts at: 3785 3785
Size: 5606
NsiI A_TGCA'T
Cuts at: 623 623
Size: 5606
NspI r_CATG'y
Cuts at: 1054 1528 2346 2418 2659 3159 4066 1054
Size: 474 818 72 241 500 907 2594
Fragments arranged by size:
2594 907 818 500 474 241 72
PciI A'CATG_T
Cuts at: 1050 4062 1050
Size: 3012 2594
PmeI GTTT'AAAC
Cuts at: 2605 2605
Size: 5606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 4745
Size: 32 5574
PstI C_TGCA'G
Cuts at: 2412 2653 3338 2412
Size: 241 685 4680
Fragments arranged by size:
4680 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5606
PvuII CAG'CTG
Cuts at: 2359 3031 3391 2359
Size: 672 360 4574
Fragments arranged by size:
4574 672 360
RsrII CG'GwC_CG
Cuts at: 3801 3801
Size: 5606
SacI G_AGCT'C
Cuts at: 2620 2620
Size: 5606
SalI G'TCGA_C
Cuts at: 2643 2643
Size: 5606
SapI GCTCTTCn'nnn_
Cuts at: 3629 3839 3629
Size: 210 5396
SbfI CC_TGCA'GG
Cuts at: 2653 2653
Size: 5606
ScaI AGT'ACT
Cuts at: 2211 5123 2211
Size: 2912 2694
SexAI A'CCwGG_T
Cuts at: 852 1286 852
Size: 434 5172
SfcI C'TryA_G
Cuts at: 33 1448 2216 2408 2649 3334 4327 4518 33
Size: 1415 768 192 241 685 993 191 1121
Fragments arranged by size:
1415 1121 993 768 685 241 192 191
SfoI GGC'GCC
Cuts at: 3038 3286 3038
Size: 248 5358
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5606
SmaI CCC'GGG
Cuts at: 2628 2628
Size: 5606
SmlI C'TyrA_G
Cuts at: 1789 2761 3676 4168 4430 1789
Size: 972 915 492 262 2965
Fragments arranged by size:
2965 972 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5606
SphI G_CATG'C
Cuts at: 2346 2659 3159 2346
Size: 313 500 4793
Fragments arranged by size:
4793 500 313
StyI C'CwwG_G
Cuts at: 198 378 1404 2061 2432 2740 3094 198
Size: 180 1026 657 371 308 354 2710
Fragments arranged by size:
2710 1026 657 371 354 308 180
SwaI ATTT'AAAT
Cuts at: 512 512
Size: 5606
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3189 3322 3781 3990 3189
Size: 133 459 209 4805
Fragments arranged by size:
4805 459 209 133
TatI w'GTAC_w
Cuts at: 921 945 2209 5121 921
Size: 24 1264 2912 1406
Fragments arranged by size:
2912 1406 1264 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 5123 5155 481
Size: 32 4610 32 932
Fragments arranged by size:
4610 932 32 32
Tth111I GACn'n_nGTC
Cuts at: 3403 3403
Size: 5606
XbaI T'CTAG_A
Cuts at: 2637 2637
Size: 5606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1498 1528 1775 3101 1498
Size: 30 247 1326 4003
Fragments arranged by size:
4003 1326 247 30
XmaI C'CCGG_G
Cuts at: 2626 2626
Size: 5606
XmnI GAAnn'nnTTC
Cuts at: 212 1740 212
Size: 1528 4078
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BcgI BmgBI BmtI BpmI BsiWI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI XhoI
ZraI