Restriction map for pF1KA1238
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# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbvCI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MroI        MunI        NarI        NdeI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tth111I     
XcmI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII         479  CGCG                 CG^CG
AcsI          417  AAATTT               [GA]^AATT[CT]
AflIII        612  ACGTGT               A^C[GA][CT]GT
AhdI           36  GACAGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          182  GGATC                GGATC(4/5)
ApoI          417  AAATTT               [GA]^AATT[CT]
AspEI          36  GACAGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         714  GTGCTC               G[AT]GC[AT]^C
AvaI          512  CTCGAG               C^[CT]CG[GA]G
AvaII        1000  GGTCC                G^G[AT]CC
BanI          643  GGCACC               G^G[CT][GA]CC
BbsI           55  GAAGAC               GAAGAC(2/6)
BcgI          414  CGAAAATTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         390  GGATAC               GTATCC(6/5)
BcnI          938  CCCGG                CC^[GC]GG
BmyI          714  GTGCTC               G[AGT]GC[ACT]^C
BpmI          451  CTGGAG               CTGGAG(16/14)
BpuAI          55  GAAGAC               GAAGAC(2/6)
BsaAI         611  TACGTG               [CT]AC^GT[GA]
BseRI         106  GAGGAG               GAGGAG(10/8)
BsiHKAI       714  GTGCTC               G[AT]GC[AT]^C
BsmI          674  GAATGC               GAATGC(1/-1)
BsoBI         512  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      714  GTGCTC               G[AGT]GC[ACT]^C
BspMI         791  ACCTGC               ACCTGC(4/8)
BsrFI         929  GCCGGC               [GA]^CCGG[CT]
BstAPI        898  GCAGACTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         479  CGCG                 CG^CG
BstXI         235  CCAACCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        929  GCCGGC               [GA]^CCGG[CT]
DdeI          538  CTAAG                C^T[ACGT]AG
Eam1105I       36  GACAGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI          480  GCGGG                CCCGC(4/6)
HaeII         561  AGCGCC               [GA]GCGC^[CT]
HgaI          821  GCGTC                GACGC(5/10)
HinfI         592  GAGTC                G^A[ACGT]TC
MslI          618  CATTGTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1123  CAGCTG               C[AC]G^C[GT]G
MvnI          479  CGCG                 CG^CG
NaeI          929  GCCGGC               GCC^GGC
NciI          938  CCCGG                CC^[GC]GG
NcoI          372  CCATGG               C^CATGG
NgoMIV        929  GCCGGC               G^CCGGC
PaeR7I        512  CTCGAG               C^TCGAG
PflMI          20  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          592  GAGTC                GAGTC(4/5)
PpuMI         999  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII        1123  CAGCTG               CAG^CTG
SfaNI          47  GCATC                GCATC(5/9)
SpeI          982  ACTAGT               A^CTAGT
Tsp509I       418  AATT                 ^AATT
Van91I         20  CCAAGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          512  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          232  GTAC                 GT^AC
              610  GTAC                
BalI          369  TGGCCA               TGG^CCA
              408  TGGCCA              
BsmAI         979  GAGAC                GTCTC(1/5)
              594  GTCTC               
BsmFI         948  GTCCC                GGGAC(10/14)
             1001  GTCCC               
CfoI          276  GCGC                 GCG^C
              562  GCGC                
Cfr13I       1000  GGTCC                G^G[ACGT]CC
             1104  GGGCC               
DraII         999  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1103  GGGGCCT             
DsaI          372  CCATGG               C^C[GA][CT]GG
              959  CCGTGG              
EcoO109I      999  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1103  GGGGCCT             
EcoT14I       241  CCTTGG               C^C[AT][AT]GG
              372  CCATGG              
HhaI          276  GCGC                 GCG^C
              562  GCGC                
HinP1I        276  GCGC                 G^CGC
              562  GCGC                
HincII       1021  GTTGAC               GT[CT]^[GA]AC
             1051  GTTGAC              
HindII       1021  GTTGAC               GT[CT]^[GA]AC
             1051  GTTGAC              
MaeII         612  ACGT                 A^CGT
              654  ACGT                
MboII          55  GAAGA                GAAGA(8/7)
              288  GAAGA               
MluNI         369  TGGCCA               TGG^CCA
              408  TGGCCA              
MscI          369  TGGCCA               TGG^CCA
              408  TGGCCA              
NheI          741  GCTAGC               G^CTAGC
             1128  GCTAGC              
RsaI          232  GTAC                 GT^AC
              610  GTAC                
Sau96I       1000  GGTCC                G^G[ACGT]CC
             1104  GGGCC               
SmlI          317  CTCAAG               C^T[CT][GA]AG
              512  CTCGAG              
StyI          241  CCTTGG               C^C[AT][AT]GG
              372  CCATGG              
TaqI          158  TCGA                 T^CGA
              513  TCGA                
Tsp45I        260  GTGAC                ^GT[GC]AC
              485  GTCAC               
TthHB8I       158  TCGA                 T^CGA
              513  TCGA                
XbaI          282  TCTAGA               T^CTAGA
              876  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      ScrFI       
Tru9I       TseI        TspRI       
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