Restriction map for pF1KA1230
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# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       KasI        KpnI        
MluI        MroI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RsrII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XmaCI       XmaI        


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# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI         2363  GTCTAC               GT^[AC][GT]AC
AccII        1866  CGCG                 CG^CG
AclI         3028  AACGTT               AA^CGTT
AgeI         2870  ACCGGT               A^CCGGT
AhdI         2269  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1619  GTGCAC               G^TGCAC
AocI         2334  CCTGAGG              CC^T[ACGT]AGG
ApaI         1870  GGGCCC               GGGCC^C
ApaLI        1619  GTGCAC               G^TGCAC
AspEI        2269  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1889  CTCGAG               C^[CT]CG[GA]G
BanI         1333  GGTGCC               G^G[CT][GA]CC
BbvCI        2626  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1578  CCGGG                CC^[GC]GG
BglI         1509  GCCCCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         298  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1886  GGTCTC               GGTCTC(1/5)
BsaWI        2870  ACCGGT               [AT]^CCGG[AT]
BsgI          145  CTGCAC               GTGCAG(16/14)
BsoBI        1889  CTCGAG               C^[CT]CG[GA]G
BspHI        1766  TCATGA               T^CATGA
BsrFI        2870  ACCGGT               [GA]^CCGG[CT]
BstAPI       2464  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1866  CGCG                 CG^CG
BstXI        1391  CCAGCAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2334  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2870  ACCGGT               [GA]^CCGG[CT]
DraIII        148  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2269  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2334  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2847  CCTGTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         1867  GCGGG                CCCGC(4/6)
HgaI         2946  GACGC                GACGC(5/10)
HindIII       679  AAGCTT               A^AGCTT
HpaI          366  GTTAAC               GTT^AAC
KspI         1865  CCGCGG               CCGC^GG
MamI          298  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1959  CAATTG               C^AATTG
MslI         1524  CACCTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1959  CAATTG               C^AATTG
MvnI         1866  CGCG                 CG^CG
NciI         1578  CCGGG                CC^[GC]GG
NheI         2106  GCTAGC               G^CTAGC
PaeR7I       1889  CTCGAG               C^TCGAG
PflMI        2115  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2870  ACCGGT               A^CCGGT
Psp1406I     3028  AACGTT               AA^CGTT
PvuII         551  CAGCTG               CAG^CTG
RcaI         1766  TCATGA               T^CATGA
SacII        1865  CCGCGG               CCGC^GG
SspI           80  AATATT               AAT^ATT
Van91I       2115  CCAAGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1889  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BseRI        1086  CTCCTC               GAGGAG(10/8)
             1847  GAGGAG              
EcoRI        1837  GAATTC               G^AATTC
             1995  GAATTC              
HincII        366  GTTAAC               GT[CT]^[GA]AC
             1278  GTTGAC              
HindII        366  GTTAAC               GT[CT]^[GA]AC
             1278  GTTGAC              
HphI         2761  GGTGA                GGTGA(8/7)
             1135  TCACC               
PpuMI        1492  GGGACCC              [GA]G^G[AT]CC[CT]
             2330  AGGTCCT             
SacI          140  GAGCTC               GAGCT^C
             1466  GAGCTC              
SapI          424  GAAGAGC              GCTCTTC(1/4)
             1060  GAAGAGC             
SexAI         374  ACCAGGT              A^CC[AT]GGT
             2829  ACCTGGT             
SmlI          207  CTTGAG               C^T[CT][GA]AG
             1889  CTCGAG              
XbaI         2055  TCTAGA               T^CTAGA
             2517  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspLU11I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI        
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