Restriction map for
pF1KA1190
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HinfI
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AspI 2074 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1157 GGCGCC GGCGC^C
BbsI 1869 GAAGAC GAAGAC(2/6)
BglI 373 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 267 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 267 GCTCAGC GC^T[ACGT]AGC
BpuAI 1869 GAAGAC GAAGAC(2/6)
BsaI 1651 GAGACC GGTCTC(1/5)
BspLU11I 1480 ACATGT A^CATGT
BstXI 230 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 267 GCTCAGC GC^T[ACGT]AGC
HincII 241 GTCAAC GT[CT]^[GA]AC
HindII 241 GTCAAC GT[CT]^[GA]AC
KasI 1157 GGCGCC G^GCGCC
MseI 566 TTAA T^TAA
NarI 1157 GGCGCC GG^CGCC
NcoI 1592 CCATGG C^CATGG
NheI 102 GCTAGC G^CTAGC
PflFI 2074 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1029 GAGCTC GAGCT^C
ScaI 1850 AGTACT AGT^ACT
SfoI 1157 GGCGCC GGC^GCC
SgrAI 1948 CACCGGCG C[GA]^CCGG[CT]G
SphI 1382 GCATGC GCATG^C
Tru9I 566 TTAA T^TAA
Tth111I 2074 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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Acc65I 1165 GGTACC G^GTACC
1346 GGTACC
Alw44I 1534 GTGCAC G^TGCAC
1693 GTGCAC
AlwI 207 GATCC GGATC(4/5)
1020 GGATC
ApaI 474 GGGCCC GGGCC^C
604 GGGCCC
ApaLI 1534 GTGCAC G^TGCAC
1693 GTGCAC
Asp718I 1165 GGTACC G^GTACC
1346 GGTACC
BbrPI 736 CACGTG CAC^GTG
1600 CACGTG
BsaAI 736 CACGTG [CT]AC^GT[GA]
1600 CACGTG
BsmAI 819 GAGAC GTCTC(1/5)
1651 GAGAC
BsrBI 333 CCGCTC CCGCTC(-3/-3)
2191 CCGCTC
BsrDI 281 CATTGC GCAATG(2/0)
1742 GCAATG
BssSI 259 CACGAG CACGAG(-5/-1)
1325 CACGAG
DraIII 188 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
259 CACGAGGTG
HindIII 1490 AAGCTT A^AGCTT
1501 AAGCTT
KpnI 1165 GGTACC GGTAC^C
1346 GGTACC
PflMI 1216 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2058 CCACACCCTGG
PmaCI 736 CACGTG CAC^GTG
1600 CACGTG
PmlI 736 CACGTG CAC^GTG
1600 CACGTG
SapI 652 GAAGAGC GCTCTTC(1/4)
2193 GCTCTTC
SexAI 189 ACCTGGT A^CC[AT]GGT
348 ACCAGGT
SfaNI 1237 GCATC GCATC(5/9)
1787 GCATC
SmaI 1142 CCCGGG CCC^GGG
2139 CCCGGG
SmlI 1687 CTCAAG C^T[CT][GA]AG
2023 CTCAAG
StuI 447 AGGCCT AGG^CCT
2094 AGGCCT
TaqI 590 TCGA T^CGA
1856 TCGA
TthHB8I 590 TCGA T^CGA
1856 TCGA
Van91I 1216 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2058 CCACACCCTGG
XmaCI 1142 CCCGGG C^CCGGG
2139 CCCGGG
XmaI 1142 CCCGGG C^CCGGG
2139 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI Aor51HI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI TseI Tsp45I
TspRI XcmI XhoII XmnI