Restriction map for pF1KA1189
 (Linear) (Six-base) MAPSORT of: KIAA1189.seq  Check: 2361  from: 1  to: 852



KIAA1189.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AflIII A'CryG_T

Cuts at:      0     506     852 
   Size:        506     346
 
ApaLI G'TGCA_C

Cuts at:      0     152     852 
   Size:        152     700
 
ApoI r'AATT_y

Cuts at:      0     613     829     852 
   Size:        613     216      23

  Fragments arranged by size: 

                 613     216      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     390     852 
   Size:        390     462
 
BfrBI ATG'CAT

Cuts at:      0     846     852 
   Size:        846       6
 
BglII A'GATC_T

Cuts at:      0     285     852 
   Size:        285     567
 
Bme1580I G_kGCm'C

Cuts at:      0     156     852 
   Size:        156     696
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     134     852 
   Size:        134     718
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735     852 
   Size:        735     117
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     544     852 
   Size:        544     308
 
BseYI C'CCAG_C

Cuts at:      0     477     852 
   Size:        477     375
 
BsiHKAI G_wGCw'C

Cuts at:      0     156     852 
   Size:        156     696
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     852 
   Size:        156     696
 
BstBI TT'CG_AA

Cuts at:      0     486     852 
   Size:        486     366
 
BstEII G'GTnAC_C

Cuts at:      0     451     852 
   Size:        451     401
 
BstYI r'GATC_y

Cuts at:      0     285     852 
   Size:        285     567
 
Bsu36I CC'TnA_GG

Cuts at:      0     729     852 
   Size:        729     123
 
BtgI C'CryG_G

Cuts at:      0     218     852 
   Size:        218     634
 
BtsI GCAGTG_nn'

Cuts at:      0     693     852 
   Size:        693     159
 
DraI TTT'AAA

Cuts at:      0     648     852 
   Size:        648     204
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     626     733     852 
   Size:        245     381     107     119

  Fragments arranged by size: 

                 381     245     119     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735     852 
   Size:        735     117
 
EcoRI G'AATT_C

Cuts at:      0     829     852 
   Size:        829      23
 
EcoRV GAT'ATC

Cuts at:      0     747     852 
   Size:        747     105
 
HincII GTy'rAC

Cuts at:      0      67     852 
   Size:         67     785
 
MspA1I CmG'CkG

Cuts at:      0     463     852 
   Size:        463     389
 
NcoI C'CATG_G

Cuts at:      0     218     852 
   Size:        218     634
 
NsiI A_TGCA'T

Cuts at:      0     848     852 
   Size:        848       4
 
NspI r_CATG'y

Cuts at:      0     204     510     852 
   Size:        204     306     342

  Fragments arranged by size: 

                 342     306     204
 
PciI A'CATG_T

Cuts at:      0     506     852 
   Size:        506     346
 
PvuII CAG'CTG

Cuts at:      0     463     852 
   Size:        463     389
 
SfcI C'TryA_G

Cuts at:      0     126     852 
   Size:        126     726
 
SpeI A'CTAG_T

Cuts at:      0     304     852 
   Size:        304     548
 
StyI C'CwwG_G

Cuts at:      0     218     852 
   Size:        218     634
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     726     758     852 
   Size:        726      32      94

  Fragments arranged by size: 

                 726      94      32
 

 Enzymes that do cut:

   AflIII    ApaLI     ApoI     BbsI    BfrBI    BglII Bme1580I     BmrI
     BpmI    BseRI    BseYI  BsiHKAI Bsp1286I    BstBI   BstEII    BstYI
   Bsu36I     BtgI     BtsI     DraI     EarI  Eco57MI    EcoRI    EcoRV
   HincII   MspA1I     NcoI     NsiI     NspI     PciI    PvuII     SfcI
     SpeI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstXI  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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