Restriction map for pF1KA1161
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        AocI        ApaI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BspHI       BsrDI       BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraII       DraIII      
Eam1105I    EarI        Eco81I      EcoO109I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        Ksp632I     MamI        
MfeI        MluI        MroI        MseI        MunI        
NcoI        NdeI        NheI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TfiI        Tru9I       XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI         1003  AAATTC               [GA]^AATT[CT]
Alw44I       1069  GTGCAC               G^TGCAC
AlwNI        1347  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1069  GTGCAC               G^TGCAC
ApoI         1003  AAATTC               [GA]^AATT[CT]
AscI          872  GGCGCGCC             GG^CGCGCC
AspI          358  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          226  GTCTTC               GAAGAC(2/6)
BfaI         1195  CTAG                 C^TAG
BlpI          732  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      732  GCTGAGC              GC^T[ACGT]AGC
BpuAI         226  GTCTTC               GAAGAC(2/6)
BsaI          839  GAGACC               GGTCTC(1/5)
BsmBI         135  CGTCTC               CGTCTC(1/5)
BsrBI         243  CCGCTC               CCGCTC(-3/-3)
BstYI         914  AGATCC               [GA]^GATC[CT]
CelII         732  GCTGAGC              GC^T[ACGT]AGC
DrdI         1342  GACCCCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         141  CCTACGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       353  CCAAGG               C^C[AT][AT]GG
MaeI         1195  CTAG                 C^TAG
MflI          914  AGATCC               [GA]^GATC[CT]
NotI          598  GCGGCCGC             GC^GGCCGC
NruI         1458  TCGCGA               TCG^CGA
PaeR7I        571  CTCGAG               C^TCGAG
PflFI         358  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1346  CCAGCGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          270  CTGCAG               CTGCA^G
SacI         1612  GAGCTC               GAGCT^C
SalI         2002  GTCGAC               G^TCGAC
SgrAI        1976  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         1523  GCATGC               GCATG^C
StuI          830  AGGCCT               AGG^CCT
StyI          353  CCAAGG               C^C[AT][AT]GG
Tsp509I      1004  AATT                 ^AATT
Tth111I       358  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1346  CCAGCGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          447  CCACTGGTATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          571  CTCGAG               C^TCGAG
XhoII         914  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AatII         760  GACGTC               GACGT^C
             1918  GACGTC              
AccI          538  GTCTAC               GT^[AC][GT]AC
             2002  GTCGAC              
AflIII        959  ACATGT               A^C[GA][CT]GT
             1022  ACATGT              
AlwI          915  GATCC                GGATC(4/5)
             1455  GGATC               
Aor51HI       575  AGCGCT               AGC^GCT
              656  AGCGCT              
BalI          501  TGGCCA               TGG^CCA
             1700  TGGCCA              
BbeI          283  GGCGCC               GGCGC^C
              460  GGCGCC              
BpmI         1353  CTGGAG               CTGGAG(16/14)
             1894  CTGGAG              
BseRI         543  CTCCTC               GAGGAG(10/8)
              388  GAGGAG              
BsgI          325  CTGCAC               GTGCAG(16/14)
              922  CTGCAC              
Bsp1407I        2  TGTACA               T^GTACA
              962  TGTACA              
BspLU11I      959  ACATGT               A^CATGT
             1022  ACATGT              
BsrGI           2  TGTACA               T^GTACA
              962  TGTACA              
Eco47III      575  AGCGCT               AGC^GCT
              656  AGCGCT              
HincII       1081  GTCAAC               GT[CT]^[GA]AC
             2002  GTCGAC              
HindII       1081  GTCAAC               GT[CT]^[GA]AC
             2002  GTCGAC              
KasI          283  GGCGCC               G^GCGCC
              460  GGCGCC              
MaeII         761  ACGT                 A^CGT
             1919  ACGT                
MluNI         501  TGGCCA               TGG^CCA
             1700  TGGCCA              
MscI          501  TGGCCA               TGG^CCA
             1700  TGGCCA              
NarI          283  GGCGCC               GG^CGCC
              460  GGCGCC              
SfaNI         566  GCATC                GCATC(5/9)
             1181  GCATC               
SfcI          129  CTACAG               C^T[GA][CT]AG
              270  CTGCAG              
SfoI          283  GGCGCC               GGC^GCC
              460  GGCGCC              
SmlI          571  CTCGAG               C^T[CT][GA]AG
             1756  CTTGAG              
SspBI           2  TGTACA               T^GTACA
              962  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BglI        BmyI        BsaHI       
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssHII      BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EagI        EclXI       
Eco52I      EcoO65I     EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KspI        MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        RsaI        SacII       Sau3AI      Sau96I      
ScrFI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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