Restriction map for pF1KA1161
 (Linear) (Six-base) MAPSORT of: KIAA1161.seq  Check: 8821  from: 1  to: 2040



KIAA1161.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      39     978    1247    2040 
   Size:         39     939     269     793

  Fragments arranged by size: 

                 939     793     269      39
 
AatII G_ACGT'C

Cuts at:      0     764    1922    2040 
   Size:        764    1158     118

  Fragments arranged by size: 

                1158     764     118
 
AccI GT'mk_AC

Cuts at:      0     539    2003    2040 
   Size:        539    1464      37

  Fragments arranged by size: 

                1464     539      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     655    1302    2040 
   Size:         79     576     647     738

  Fragments arranged by size: 

                 738     647     576      79
 
AfeI AGC'GCT

Cuts at:      0     577     658    2040 
   Size:        577      81    1382

  Fragments arranged by size: 

                1382     577      81
 
AflIII A'CryG_T

Cuts at:      0     959    1022    2040 
   Size:        959      63    1018

  Fragments arranged by size: 

                1018     959      63
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1588    2040 
   Size:       1588     452
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1352    2040 
   Size:       1352     688
 
ApaLI G'TGCA_C

Cuts at:      0    1069    2040 
   Size:       1069     971
 
ApoI r'AATT_y

Cuts at:      0    1003    2040 
   Size:       1003    1037
 
AscI GG'CGCG_CC

Cuts at:      0     873    2040 
   Size:        873    1167
 
AvaI C'yCGr_G

Cuts at:      0     571     826    1603    2040 
   Size:        571     255     777     437

  Fragments arranged by size: 

                 777     571     437     255
 
BanI G'GyrC_C

Cuts at:      0     283     460     816    1743    2040 
   Size:        283     177     356     927     297

  Fragments arranged by size: 

                 927     356     297     283     177
 
BanII G_rGCy'C

Cuts at:      0      76     515     757    1616    2040 
   Size:         76     439     242     859     424

  Fragments arranged by size: 

                 859     439     424     242      76
 
BbeI G_GCGC'C

Cuts at:      0     287     464    2040 
   Size:        287     177    1576

  Fragments arranged by size: 

                1576     287     177
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     219    2040 
   Size:        219    1821
 
BglI GCCn_nnn'nGGC

Cuts at:      0     281     500    1729    2040 
   Size:        281     219    1229     311

  Fragments arranged by size: 

                1229     311     281     219
 
BlpI GC'TnA_GC

Cuts at:      0     733    2040 
   Size:        733    1307
 
Bme1580I G_kGCm'C

Cuts at:      0     629    1073    1577    1604    2040 
   Size:        629     444     504      27     436

  Fragments arranged by size: 

                 629     504     444     436      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1374    1915    2040 
   Size:       1374     541     125

  Fragments arranged by size: 

                1374     541     125
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1777    2040 
   Size:       1777     263
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     833    2040 
   Size:        833    1207
 
BsaHI Gr'CG_yC

Cuts at:      0     284     461     551     761    1043    1919    1975    2035 
   Size:        284     177      90     210     282     876      56      60

Cuts at:   2035    2040 
   Size:          5

  Fragments arranged by size: 

                 876     284     282     210     177      90      60      56
                   5
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     121     151     209     239     250     280     751     781 
   Size:        121      30      58      30      11      30     471      30

Cuts at:    781    2040 
   Size:       1259

  Fragments arranged by size: 

                1259     471     121      58      30      30      30      30
                  11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     403     534    2040 
   Size:        403     131    1506

  Fragments arranged by size: 

                1506     403     131
 
BseYI C'CCAG_C

Cuts at:      0      69     291     383     515    1345    1527    1577    2040 
   Size:         69     222      92     132     830     182      50     463

  Fragments arranged by size: 

                 830     463     222     182     132      92      69      50
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     907    2040 
   Size:        310     597    1133

  Fragments arranged by size: 

                1133     597     310
 
BsiEI CG_ry'CG

Cuts at:      0     311     602     714    1333    2003    2040 
   Size:        311     291     112     619     670      37

  Fragments arranged by size: 

                 670     619     311     291     112      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     419    1073    1514    1616    2040 
   Size:        419     654     441     102     424

  Fragments arranged by size: 

                 654     441     424     419     102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     141    2040 
   Size:        141    1899
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     419     515     629     757    1073    1514    1577 
   Size:         76     343      96     114     128     316     441      63

Cuts at:   1577    1604    1616    2040 
   Size:         27      12     424

  Fragments arranged by size: 

                 441     424     343     316     128     114      96      76
                  63      27      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      39     213     978    1164    1247    1313    2040 
   Size:         39     174     765     186      83      66     727

  Fragments arranged by size: 

                 765     727     186     174      83      66      39
 
BsrBI CCG'CTC

Cuts at:      0     245    2040 
   Size:        245    1795
 
BsrFI r'CCGG_y

Cuts at:      0     709    1045    1148    1328    1358    1588    1650    1933 
   Size:        709     336     103     180      30     230      62     283

Cuts at:   1933    1977    2040 
   Size:         44      63

  Fragments arranged by size: 

                 709     336     283     230     180     103      63      62
                  44      30
 
BsrGI T'GTAC_A

Cuts at:      0       2     962    2040 
   Size:          2     960    1078

  Fragments arranged by size: 

                1078     960       2
 
BssHII G'CGCG_C

Cuts at:      0     251     594     676     873    2040 
   Size:        251     343      82     197    1167

  Fragments arranged by size: 

                1167     343     251     197      82
 
BstEII G'GTnAC_C

Cuts at:      0    1151    1773    1786    2040 
   Size:       1151     622      13     254

  Fragments arranged by size: 

                1151     622     254      13
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     454     495    2040 
   Size:        429      25      41    1545

  Fragments arranged by size: 

                1545     429      41      25
 
BstYI r'GATC_y

Cuts at:      0     914    2040 
   Size:        914    1126
 
BtgI C'CryG_G

Cuts at:      0      77     107     410     878    1142    1265    1309    1505 
   Size:         77      30     303     468     264     123      44     196

Cuts at:   1505    2040 
   Size:        535

  Fragments arranged by size: 

                 535     468     303     264     196     123      77      44
                  30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     293     548    1610    1689    2040 
   Size:        293     255    1062      79     351

  Fragments arranged by size: 

                1062     351     293     255      79
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1348    2040 
   Size:       1348     692
 
EaeI y'GGCC_r

Cuts at:      0     104     308     501     599     711    1330    1637    1652 
   Size:        104     204     193      98     112     619     307      15

Cuts at:   1652    1700    2040 
   Size:         48     340

  Fragments arranged by size: 

                 619     340     307     204     193     112     104      98
                  48      15
 
EagI C'GGCC_G

Cuts at:      0     308     599     711    1330    2040 
   Size:        308     291     112     619     710

  Fragments arranged by size: 

                 710     619     308     291     112
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     166     220     481     907    1018    1414    1435    2040 
   Size:        166      54     261     426     111     396      21     605

  Fragments arranged by size: 

                 605     426     396     261     166     111      54      21
            
 
EcoICRI GAG'CTC

Cuts at:      0    1614    2040 
   Size:       1614     426
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     655    1302    1374    1915    2040 
   Size:         79     576     647      72     541     125

  Fragments arranged by size: 

                 647     576     541     125      79      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     145    2040 
   Size:        145    1895
 
HaeII r_GCGC'y

Cuts at:      0     287     400     464     579     660     727     865    1365 
   Size:        287     113      64     115      81      67     138     500

Cuts at:   1365    1381    1683    2040 
   Size:         16     302     357

  Fragments arranged by size: 

                 500     357     302     287     138     115     113      81
                  67      64      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     209     241     250     282     526     558 
   Size:         10      32     167      32       9      32     244      32

Cuts at:    558     751     783    1447    1479    2040 
   Size:        193      32     664      32     561

  Fragments arranged by size: 

                 664     561     244     193     167      32      32      32
                  32      32      32      10       9
 
HincII GTy'rAC

Cuts at:      0    1083    2004    2040 
   Size:       1083     921      36

  Fragments arranged by size: 

                1083     921      36
 
KasI G'GCGC_C

Cuts at:      0     283     460    2040 
   Size:        283     177    1580

  Fragments arranged by size: 

                1580     283     177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    2040 
   Size:        572    1468
 
MscI TGG'CCA

Cuts at:      0     503    1702    2040 
   Size:        503    1199     338

  Fragments arranged by size: 

                1199     503     338
 
MslI CAynn'nnrTG

Cuts at:      0     364     452    1372    2040 
   Size:        364      88     920     668

  Fragments arranged by size: 

                 920     668     364      88
 
MspA1I CmG'CkG

Cuts at:      0      66      79     109     324    1311    1335    1507    1581 
   Size:         66      13      30     215     987      24     172      74

Cuts at:   1581    1749    2040 
   Size:        168     291

  Fragments arranged by size: 

                 987     291     215     172     168      74      66      30
                  24      13
 
NaeI GCC'GGC

Cuts at:      0     711    1047    1360    1590    1652    1935    2040 
   Size:        711     336     313     230      62     283     105

  Fragments arranged by size: 

                 711     336     313     283     230     105      62
 
NarI GG'CG_CC

Cuts at:      0     284     461    2040 
   Size:        284     177    1579

  Fragments arranged by size: 

                1579     284     177
 
NgoMIV G'CCGG_C

Cuts at:      0     709    1045    1358    1588    1650    1933    2040 
   Size:        709     336     313     230      62     283     107

  Fragments arranged by size: 

                 709     336     313     283     230     107      62
 
NotI GC'GGCC_GC

Cuts at:      0     599    2040 
   Size:        599    1441
 
NruI TCG'CGA

Cuts at:      0    1460    2040 
   Size:       1460     580
 
NspI r_CATG'y

Cuts at:      0     963    1026    1527    2040 
   Size:        963      63     501     513

  Fragments arranged by size: 

                 963     513     501      63
 
PciI A'CATG_T

Cuts at:      0     959    1022    2040 
   Size:        959      63    1018

  Fragments arranged by size: 

                1018     959      63
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1352    2040 
   Size:       1352     688
 
PspXI vC'TCGA_Gb

Cuts at:      0     571    2040 
   Size:        571    1469
 
PstI C_TGCA'G

Cuts at:      0     274    2040 
   Size:        274    1766
 
SacI G_AGCT'C

Cuts at:      0    1616    2040 
   Size:       1616     424
 
SacII CC_GC'GG

Cuts at:      0      80     110    1312    1508    2040 
   Size:         80      30    1202     196     532

  Fragments arranged by size: 

                1202     532     196      80      30
 
SalI G'TCGA_C

Cuts at:      0    2002    2040 
   Size:       2002      38
 
SfcI C'TryA_G

Cuts at:      0     129     270    2040 
   Size:        129     141    1770

  Fragments arranged by size: 

                1770     141     129
 
SfoI GGC'GCC

Cuts at:      0     285     462    2040 
   Size:        285     177    1578

  Fragments arranged by size: 

                1578     285     177
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1977    2040 
   Size:       1977      63
 
SmlI C'TyrA_G

Cuts at:      0     571    1756    2040 
   Size:        571    1185     284

  Fragments arranged by size: 

                1185     571     284
 
SphI G_CATG'C

Cuts at:      0    1527    2040 
   Size:       1527     513
 
StuI AGG'CCT

Cuts at:      0     832    2040 
   Size:        832    1208
 
StyI C'CwwG_G

Cuts at:      0     353    2040 
   Size:        353    1687
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1056    1223    2040 
   Size:       1056     167     817

  Fragments arranged by size: 

                1056     817     167
 
TatI w'GTAC_w

Cuts at:      0       2     779     962    2040 
   Size:          2     777     183    1078

  Fragments arranged by size: 

                1078     777     183       2
 
Tth111I GACn'n_nGTC

Cuts at:      0     361    2040 
   Size:        361    1679
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     454    2040 
   Size:        454    1586
 
XhoI C'TCGA_G

Cuts at:      0     571    2040 
   Size:        571    1469
 
ZraI GAC'GTC

Cuts at:      0     762    1920    2040 
   Size:        762    1158     120

  Fragments arranged by size: 

                1158     762     120
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AlfI    AlwNI
    ApaLI     ApoI     AscI     AvaI     BanI    BanII     BbeI     BbsI
     BglI     BlpI Bme1580I     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstEII    BstXI    BstYI     BtgI
    BtgZI     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NotI     NruI     NspI     PciI    PflMI
    PspXI     PstI     SacI    SacII     SalI     SfcI     SfoI    SgrAI
     SmlI     SphI     StuI     StyI    TaqII     TatI  Tth111I     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AloI     ApaI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     EarI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NcoI
     NdeI     NheI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII
    RsrII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI     XbaI
     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com