Restriction map for pF1KA1144
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BsaI        BsaWI       BseAI       BsgI        
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfiI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         190  GACGTC               GACGT^C
AflIII         23  ACGTGT               A^C[GA][CT]GT
AhdI          790  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          888  CCTGAGG              CC^T[ACGT]AGG
AspEI         790  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          187  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1280  TGCGCA               TGC^GCA
AvrII         673  CCTAGG               C^CTAGG
BanI         1253  GGCGCC               G^G[CT][GA]CC
BbeI         1253  GGCGCC               GGCGC^C
BcnI          538  CCCGG                CC^[GC]GG
BfaI          674  CTAG                 C^TAG
BlnI          673  CCTAGG               C^CTAGG
BpmI          166  CTGGAG               CTGGAG(16/14)
BsaAI         238  TACGTG               [CT]AC^GT[GA]
BsiEI         213  CGACCG               CG[GA][CT]^CG
BsmAI         118  GAGAC                GTCTC(1/5)
BsmBI         118  GAGACG               CGTCTC(1/5)
BsmI          771  GAATGC               GAATGC(1/-1)
BsrBI         945  CCGCTC               CCGCTC(-3/-3)
BsrDI         702  CATTGC               GCAATG(2/0)
BssSI        1198  CTCGTG               CACGAG(-5/-1)
BstAPI         96  GCACACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         679  TTCGAA               TT^CGAA
BstXI         578  CCATCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        888  CCTGAGG              CC^T[ACGT]AGG
DraI         1413  TTTAAA               TTT^AAA
Eam1105I      790  GACCTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          229  CTCTTC               CTCTTC(1/4)
Eco81I        888  CCTGAGG              CC^T[ACGT]AGG
FauI          198  GCGGG                CCCGC(4/6)
FspI         1280  TGCGCA               TGC^GCA
HindIII       265  AAGCTT               A^AGCTT
KasI         1253  GGCGCC               G^GCGCC
Ksp632I       229  CTCTTC               CTCTTC(1/4)
MaeI          674  CTAG                 C^TAG
NarI         1253  GGCGCC               GG^CGCC
NciI          538  CCCGG                CC^[GC]GG
NcoI          369  CCATGG               C^CATGG
NspV          679  TTCGAA               TT^CGAA
PflFI         187  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          346  GACTC                GAGTC(4/5)
PstI          354  CTGCAG               CTGCA^G
SapI          228  GCTCTTC              GCTCTTC(1/4)
ScaI          317  AGTACT               AGT^ACT
SexAI         833  ACCTGGT              A^CC[AT]GGT
SfoI         1253  GGCGCC               GGC^GCC
SfuI          679  TTCGAA               TT^CGAA
TfiI         1420  GATTC                G^A[AT]TC
Tsp509I      1327  AATT                 ^AATT
Tth111I       187  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          318  GTAC                 GT^AC
             1128  GTAC                
AlwNI          10  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              883  CAGGTCCTG           
AvaI          115  CCCGAG               C^[CT]CG[GA]G
              388  CCCGAG              
BlpI          280  GCTGAGC              GC^T[ACGT]AGC
              552  GCTGAGC             
Bpu1102I      280  GCTGAGC              GC^T[ACGT]AGC
              552  GCTGAGC             
BseRI          81  GAGGAG               GAGGAG(10/8)
             1057  GAGGAG              
BsoBI         115  CCCGAG               C^[CT]CG[GA]G
              388  CCCGAG              
BsrI          320  ACTGG                ACTGG(1/-1)
              937  ACTGG               
CelII         280  GCTGAGC              GC^T[ACGT]AGC
              552  GCTGAGC             
DraIII        606  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1397  CACCAAGTG           
EcoT14I       369  CCATGG               C^C[AT][AT]GG
              673  CCTAGG              
HaeII         525  GGCGCT               [GA]GCGC^[CT]
             1253  GGCGCC              
HgaI          120  GACGC                GACGC(5/10)
              469  GACGC               
HinfI         346  GACTC                G^A[ACGT]TC
             1420  GATTC               
MaeIII        416  GTGAC                ^GT[ACGT]AC
             1289  GTGAC               
PvuII         514  CAGCTG               CAG^CTG
             1284  CAGCTG              
RsaI          318  GTAC                 GT^AC
             1128  GTAC                
SfcI          354  CTGCAG               C^T[GA][CT]AG
              360  CTACAG              
SmlI          757  CTCAAG               C^T[CT][GA]AG
             1267  CTTGAG              
StyI          369  CCATGG               C^C[AT][AT]GG
              673  CCTAGG              
Tsp45I        416  GTGAC                ^GT[GC]AC
             1289  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaII       BalI        BanII       BbsI        
BbvI        BmyI        BpuAI       BsaBI       BsaHI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrFI       BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MaeII       MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PpuMI       SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        Tru9I       TseI        
TspRI       TthHB8I     XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com