Restriction map for pF1KA1132
 (Circular) (Six-base) MAPSORT of: pF1KA1132.seq  Check: 5263  from: 1  to: 9272



pF1KA1132  9272 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    198    5388     198 
   Size:       5190    4082
 
AccI GT'mk_AC

Cuts at:   3257    6310    3257 
   Size:       3053    6219
 
Acc65I G'GTAC_C

Cuts at:    284    6288     284 
   Size:       6004    3268
 
AclI AA'CG_TT

Cuts at:   9112    9112 
   Size:       9272
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     542    1099    1332    2683    2685    4533    4990    7097 
   Size:        360     557     233    1351       2    1848     457    2107

Cuts at:   7097    7529    8276    8544     182 
   Size:        432     747     268     910

  Fragments arranged by size: 

                2107    1848    1351     910     747     557     457     432
                 360     268     233       2
 
AfeI AGC'GCT

Cuts at:   4420    6108    4420 
   Size:       1688    7584
 
AflIII A'CryG_T

Cuts at:   4190    5261    6624    7728    8705    4190 
   Size:       1071    1363    1104     977    4757

  Fragments arranged by size: 

                4757    1363    1104    1071     977
 
AgeI A'CCGG_T

Cuts at:   1556    5333    7660    1556 
   Size:       3777    2327    3168

  Fragments arranged by size: 

                3777    3168    2327
 
AleI CACnn'nnGTG

Cuts at:   1389    5041    6262    1389 
   Size:       3652    1221    4399

  Fragments arranged by size: 

                4399    3652    1221
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2836    3664    4168    2836 
   Size:        828     504    7940

  Fragments arranged by size: 

                7940     828     504
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2680    2712    2680 
   Size:         32    9240
 
AlwNI CAG_nnn'CTG

Cuts at:    769     920    2346    3609    3891    4063    5167    5698    6577 
   Size:        151    1426    1263     282     172    1104     531     879

Cuts at:   6577    8144     769 
   Size:       1567    1897

  Fragments arranged by size: 

                1897    1567    1426    1263    1104     879     531     282
                 172     151
 
ApaI G_GGCC'C

Cuts at:   1434    3074    3335    3405    5065    5988    6154    1434 
   Size:       1640     261      70    1660     923     166    4552

  Fragments arranged by size: 

                4552    1660    1640     923     261     166      70
 
ApaLI G'TGCA_C

Cuts at:   1560    4194    8042    1560 
   Size:       2634    3848    2790

  Fragments arranged by size: 

                3848    2790    2634
 
ApoI r'AATT_y

Cuts at:   1915    2053    6276    8625    8881    1915 
   Size:        138    4223    2349     256    2306

  Fragments arranged by size: 

                4223    2349    2306     256     138
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       9272
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9272
 
AvaI C'yCGr_G

Cuts at:    855    1369    1968    2028    3799    5543    6190    6292     855 
   Size:        514     599      60    1771    1744     647     102    3835

  Fragments arranged by size: 

                3835    1771    1744     647     599     514     102      60
            
 
BamHI G'GATC_C

Cuts at:   6297    6297 
   Size:       9272
 
BanI G'GyrC_C

Cuts at:    172     240     284     976    1405    1498    2428    3132    3997 
   Size:         68      44     692     429      93     930     704     865

Cuts at:   3997    4121    5647    6288    6702    6950    6985     172 
   Size:        124    1526     641     414     248      35    2459

  Fragments arranged by size: 

                2459    1526     930     865     704     692     641     429
                 414     248     124      93      68      44      35
 
BanII G_rGCy'C

Cuts at:    262     451    1129    1205    1434    1448    2065    2768    3038 
   Size:        189     678      76     229      14     617     703     270

Cuts at:   3038    3074    3335    3405    3449    4495    4544    5065    5419 
   Size:         36     261      70      44    1046      49     521     354

Cuts at:   5419    5864    5988    6154    6175    6286    7316     262 
   Size:        445     124     166      21     111    1030    2218

  Fragments arranged by size: 

                2218    1046    1030     703     678     617     521     445
                 354     270     261     229     189     166     124     111
                  76      70      49      44      36      21      14
 
BbeI G_GCGC'C

Cuts at:   1409    6706    6954    1409 
   Size:       5297     248    3727

  Fragments arranged by size: 

                5297    3727     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    528    1087    4097     528 
   Size:        559    3010    5703

  Fragments arranged by size: 

                5703    3010     559
 
BbvCI CC'TCA_GC

Cuts at:   4172    4290    4172 
   Size:        118    9154
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1674    1708    3334    3368    1674 
   Size:         34    1626      34    7578

  Fragments arranged by size: 

                7578    1626      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   7170    7931    9272    7170 
   Size:        761    1341    7170

  Fragments arranged by size: 

                7170    1341     761
 
BclI T'GATC_A

Cuts at:   2015    2477    6792    2015 
   Size:        462    4315    4495

  Fragments arranged by size: 

                4495    4315     462
 
BfrBI ATG'CAT

Cuts at:   2114    2114 
   Size:       9272
 
BglI GCCn_nnn'nGGC

Cuts at:   6118    6118 
   Size:       9272
 
BglII A'GATC_T

Cuts at:   2072    6787    8824    2072 
   Size:       4715    2037    2520

  Fragments arranged by size: 

                4715    2520    2037
 
BlpI GC'TnA_GC

Cuts at:   1204    6086    6384    1204 
   Size:       4882     298    4092

  Fragments arranged by size: 

                4882    4092     298
 
Bme1580I G_kGCm'C

Cuts at:    245     750    1119    1434    1564    1895    2174    2324    3074 
   Size:        505     369     315     130     331     279     150     750

Cuts at:   3074    3335    3377    3405    4000    4124    4198    5065    5771 
   Size:        261      42      28     595     124      74     867     706

Cuts at:   5771    5988    6154    6897    6990    8046     245 
   Size:        217     166     743      93    1056    1471

  Fragments arranged by size: 

                1471    1056     867     750     743     706     595     505
                 369     331     315     279     261     217     166     150
                 130     124      93      74      42      28
 
BmgBI CAC'GTC

Cuts at:    315     333    1797    2376    3423    5857     315 
   Size:         18    1464     579    1047    2434    3730

  Fragments arranged by size: 

                3730    2434    1464    1047     579      18
 
BmrI ACTGGGnnnn_n'

Cuts at:   3282    4794    4927    5140    6484    6565    6900    9044    3282 
   Size:       1512     133     213    1344      81     335    2144    3510

  Fragments arranged by size: 

                3510    2144    1512    1344     335     213     133      81
            
 
BmtI G_CTAG'C

Cuts at:   3722    3722 
   Size:       9272
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1206    1398    2670    5535    7639    1206 
   Size:        192    1272    2865    2104    2839

  Fragments arranged by size: 

                2865    2839    2104    1272     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2016    2806    3385    4395    4672    5587    6159    2016 
   Size:        790     579    1010     277     915     572    5129

  Fragments arranged by size: 

                5129    1010     915     790     579     572     277
 
Bpu10I CC'TnA_GC

Cuts at:   4172    4290    4172 
   Size:        118    9154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    871    1336    1768    2074    2653    3259    4426    4838    5325 
   Size:        465     432     306     579     606    1167     412     487

Cuts at:   5325    6448    7327    7819    8117     871 
   Size:       1123     879     492     298    2026

  Fragments arranged by size: 

                2026    1167    1123     879     606     579     492     487
                 465     432     412     306     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    721    6224    8976     721 
   Size:       5503    2752    1017

  Fragments arranged by size: 

                5503    2752    1017
 
BsaAI yAC'GTr

Cuts at:   1878    4193    4454    4742    6625    1878 
   Size:       2315     261     288    1883    4525

  Fragments arranged by size: 

                4525    2315    1883     288     261
 
BsaBI GATnn'nnATC

Cuts at:      6    6786    8823       6 
   Size:       6780    2037     455

  Fragments arranged by size: 

                6780    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    680    1406    3470    5210    6703    6951    7653    9149     680 
   Size:        726    2064    1740    1493     248     702    1496     803

  Fragments arranged by size: 

                2064    1740    1496    1493     803     726     702     248
            
 
BsaWI w'CCGG_w

Cuts at:    443    1556    3857    5333    5897    6461    6982    7660    7934 
   Size:       1113    2301    1476     564     564     521     678     274

Cuts at:   7934    8081    8492     443 
   Size:        147     411    1223

  Fragments arranged by size: 

                2301    1476    1223    1113     678     564     564     521
                 411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    352     382     589     619     670     700    1813    1843    4567 
   Size:         30     207      30      51      30    1113      30    2724

Cuts at:   4567    4597    6120    6150     352 
   Size:         30    1523      30    3474

  Fragments arranged by size: 

                3474    2724    1523    1113     207      51      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   3067    4399    5548    5584    3067 
   Size:       1332    1149      36    6755

  Fragments arranged by size: 

                6755    1332    1149      36
 
BseYI C'CCAG_C

Cuts at:    265    1624    1850    2303    2570    2831    3376    3625    3820 
   Size:       1359     226     453     267     261     545     249     195

Cuts at:   3820    3972    5420    6045    6063    6103    6124    6697    7455 
   Size:        152    1448     625      18      40      21     573     758

Cuts at:   7455    8032    8528     265 
   Size:        577     496    1009

  Fragments arranged by size: 

                1448    1359    1009     758     625     577     573     545
                 496     453     267     261     249     226     195     152
                  40      21      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    399     583     831    1819    1851    2520    4181     399 
   Size:        184     248     988      32     669    1661    5490

  Fragments arranged by size: 

                5490    1661     988     669     248     184      32
 
BsiEI CG_ry'CG

Cuts at:    104     254    3924    6814    6860    8068     104 
   Size:        150    3670    2890      46    1208    1308

  Fragments arranged by size: 

                3670    2890    1308    1208     150      46
 
BsiHKAI G_wGCw'C

Cuts at:   1564    2065    2234    3038    3653    4198    5245    5686    6202 
   Size:        501     169     804     615     545    1047     441     516

Cuts at:   6202    6286    7064    7254    8046    1564 
   Size:         84     778     190     792    2790

  Fragments arranged by size: 

                2790    1047     804     792     778     615     545     516
                 501     441     190     169      84
 
BsiWI C'GTAC_G

Cuts at:   3636    4941    3636 
   Size:       1305    7967
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1207    1344    2088    2658    3826    5217    1207 
   Size:        137     744     570    1168    1391    5262

  Fragments arranged by size: 

                5262    1391    1168     744     570     137
 
Bsp1286I G_dGCh'C

Cuts at:    245     262     451     750    1119    1129    1205    1434    1448 
   Size:         17     189     299     369      10      76     229      14

Cuts at:   1448    1564    1895    2065    2174    2234    2324    2768    3038 
   Size:        116     331     170     109      60      90     444     270

Cuts at:   3038    3074    3335    3377    3405    3449    3653    4000    4124 
   Size:         36     261      42      28      44     204     347     124

Cuts at:   4124    4198    4495    4544    5065    5245    5419    5686    5771 
   Size:         74     297      49     521     180     174     267      85

Cuts at:   5771    5864    5988    6154    6175    6202    6286    6897    6990 
   Size:         93     124     166      21      27      84     611      93

Cuts at:   6990    7064    7254    7316    8046     245 
   Size:         74     190      62     730    1471

  Fragments arranged by size: 

                1471     730     611     521     444     369     347     331
                 299     297     270     267     261     229     204     190
                 189     180     174     170     166     124     124     116
                 109      93      93      90      85      84      76      74
                  74      62      60      49      44      42      36      28
                  27      21      17      14      10
 
BspEI T'CCGG_A

Cuts at:    443    5897    8492     443 
   Size:       5454    2595    1223

  Fragments arranged by size: 

                5454    2595    1223
 
BspHI T'CATG_A

Cuts at:   2057    8448    2057 
   Size:       6391    2881
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    198    2828    4613    5388    5816    6322    6536    6838    7219 
   Size:       2630    1785     775     428     506     214     302     381

Cuts at:   7219     198 
   Size:       2251

  Fragments arranged by size: 

                2630    2251    1785     775     506     428     381     302
                 214
 
BsrBI CCG'CTC

Cuts at:     50    2294    2553    3061    3558    7564    7618    9102    9267 
   Size:       2244     259     508     497    4006      54    1484     165

Cuts at:   9267      50 
   Size:         55

  Fragments arranged by size: 

                4006    2244    1484     508     497     259     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:    747    1307    4794    7184     747 
   Size:        560    3487    2390    2835

  Fragments arranged by size: 

                3487    2835    2390     560
 
BsrFI r'CCGG_y

Cuts at:    433     823     896    1084    1556    3813    3919    4124    4658 
   Size:        390      73     188     472    2257     106     205     534

Cuts at:   4658    5333    6049    7270    7451    7660     433 
   Size:        675     716    1221     181     209    2045

  Fragments arranged by size: 

                2257    2045    1221     716     675     534     472     390
                 209     205     188     181     106      73
 
BsrGI T'GTAC_A

Cuts at:    653    2978     653 
   Size:       2325    6947
 
BssSI C'ACGA_G

Cuts at:   6140    7543    7901    6140 
   Size:       1403     358    7511

  Fragments arranged by size: 

                7511    1403     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3891    6649    3891 
   Size:       2758    6514
 
BstBI TT'CG_AA

Cuts at:   1913    5841    7633    1913 
   Size:       3928    1792    3552

  Fragments arranged by size: 

                3928    3552    1792
 
BstEII G'GTnAC_C

Cuts at:    210     993    1422    3120    3945     210 
   Size:        783     429    1698     825    5537

  Fragments arranged by size: 

                5537    1698     825     783     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     228     792    2955    3363    4141    4650     114 
   Size:        114     564    2163     408     778     509    4736

  Fragments arranged by size: 

                4736    2163     778     564     509     408     114
 
BstYI r'GATC_y

Cuts at:      1     440    1535    2072    2342    2465    2789    4053    6297 
   Size:        439    1095     537     270     123     324    1264    2244

Cuts at:   6297    6764    6787    7122    7368    8380    8466    8478    8824 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   8824       1 
   Size:        449

  Fragments arranged by size: 

                2244    1264    1095    1012     537     467     449     439
                 346     335     324     270     246     123      86      23
                  12
 
BtgI C'CryG_G

Cuts at:    227     254     419     642     833    1197    1418    2682    3077 
   Size:         27     165     223     191     364     221    1264     395

Cuts at:   3077    3239    3876    4112    4530    5065    5411    5870     227 
   Size:        162     637     236     418     535     346     459    3629

  Fragments arranged by size: 

                3629    1264     637     535     459     418     395     364
                 346     236     223     221     191     165     162      27
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2100    2487    2607    7222    7403    7566    2100 
   Size:        387     120    4615     181     163    3806

  Fragments arranged by size: 

                4615    3806     387     181     163     120
 
BtsI GCAGTG_nn'

Cuts at:   1590    1839    3024    3651    3729    4147    4169    4927    6515 
   Size:        249    1185     627      78     418      22     758    1588

Cuts at:   6515    1590 
   Size:       4347

  Fragments arranged by size: 

                4347    1588    1185     758     627     418     249      78
                  22
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   4194    4229    4194 
   Size:         35    9237
 
DraI TTT'AAA

Cuts at:   6271    6271 
   Size:       9272
 
DraIII CAC_nnn'GTG

Cuts at:    834    2319    2548    5606    5884     834 
   Size:       1485     229    3058     278    4222

  Fragments arranged by size: 

                4222    3058    1485     278     229
 
DrdI GACnn_nn'nnGTC

Cuts at:   2650    3253    3427    6978    7836    2650 
   Size:        603     174    3551     858    4086

  Fragments arranged by size: 

                4086    3551     858     603     174
 
EaeI y'GGCC_r

Cuts at:    251     290     530     825     894    1397    1935    2331    2471 
   Size:         39     240     295      69     503     538     396     140

Cuts at:   2471    3012    3425    3807    3902    3921    4097    4199    4910 
   Size:        541     413     382      95      19     176     102     711

Cuts at:   4910    5275    6029    6857    7031    7422    7449    8794     251 
   Size:        365     754     828     174     391      27    1345     729

  Fragments arranged by size: 

                1345     828     754     729     711     541     538     503
                 413     396     391     382     365     295     240     176
                 174     140     102      95      69      39      27      19
            
 
EagI C'GGCC_G

Cuts at:    251    3921    6857     251 
   Size:       3670    2936    2666

  Fragments arranged by size: 

                3670    2936    2666
 
EarI CTCTTCn'nnn_

Cuts at:    561    1234    2378    3342    3856    4573    4939    4945    5526 
   Size:        673    1144     964     514     717     366       6     581

Cuts at:   5526    7295    7505     561 
   Size:       1769     210    2328

  Fragments arranged by size: 

                2328    1769    1144     964     717     673     581     514
                 366     210       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    366    2359    7480    7790    7936    9082     366 
   Size:       1993    5121     310     146    1146     556

  Fragments arranged by size: 

                5121    1993    1146     556     310     146
 
EcoICRI GAG'CTC

Cuts at:   2063    3036    6284    2063 
   Size:        973    3248    5051

  Fragments arranged by size: 

                5051    3248     973
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     542    1099    1332    2016    2683    2685    2806    3385 
   Size:        360     557     233     684     667       2     121     579

Cuts at:   3385    4395    4533    4672    4990    5587    6159    7097    7529 
   Size:       1010     138     139     318     597     572     938     432

Cuts at:   7529    8276    8544     182 
   Size:        747     268     910

  Fragments arranged by size: 

                1010     938     910     747     684     667     597     579
                 572     557     432     360     318     268     233     139
                 138     121       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2383    2407    5045    5470    2383 
   Size:         24    2638     425    6185

  Fragments arranged by size: 

                6185    2638     425      24
 
EcoO109I rG'GnC_Cy

Cuts at:   1253    1431    1528    1778    3071    3331    3332    3402    3682 
   Size:        178      97     250    1293     260       1      70     280

Cuts at:   3682    3969    4670    4686    5061    5248    5984    5985    6150 
   Size:        287     701      16     375     187     736       1     165

Cuts at:   6150    6239    6411    1253 
   Size:         89     172    4114

  Fragments arranged by size: 

                4114    1293     736     701     375     287     280     260
                 250     187     178     172     165      97      89      70
                  16       1       1
 
EcoRI G'AATT_C

Cuts at:   1915    2053    6276    1915 
   Size:        138    4223    4911

  Fragments arranged by size: 

                4911    4223     138
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   7291    7291 
   Size:       9272
 
FseI GG_CCGG'CC

Cuts at:    827     900     827 
   Size:         73    9199
 
FspI TGC'GCA

Cuts at:   4756    7053    4756 
   Size:       2297    6975
 
HaeII r_GCGC'y

Cuts at:   1409    2154    4422    6110    6706    6954    7976    8955    1409 
   Size:        745    2268    1688     596     248    1022     979    1726

  Fragments arranged by size: 

                2268    1726    1688    1022     979     745     596     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    496     528     589     621     670     702    2000    2032    5240 
   Size:         32      61      32      49      32    1298      32    3208

Cuts at:   5240    5272    5611    5643    7114    7144    7146    7176     496 
   Size:         32     339      32    1471      30       2      30    2592

  Fragments arranged by size: 

                3208    2592    1471    1298     339      61      49      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1365    4236    5187    6311    1365 
   Size:       2871     951    1124    4326

  Fragments arranged by size: 

                4326    2871    1124     951
 
HindIII A'AGCT_T

Cuts at:   5028    5028 
   Size:       9272
 
KasI G'GCGC_C

Cuts at:   1405    6702    6950    1405 
   Size:       5297     248    3727

  Fragments arranged by size: 

                5297    3727     248
 
KpnI G_GTAC'C

Cuts at:    288    6292     288 
   Size:       6004    3268
 
MluI A'CGCG_T

Cuts at:   8705    8705 
   Size:       9272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1472    2772    5423    7943    8127    1472 
   Size:       1300    2651    2520     184    2617

  Fragments arranged by size: 

                2651    2617    2520    1300     184
 
MscI TGG'CCA

Cuts at:    292    3809    4912    6031    7033     292 
   Size:       3517    1103    1119    1002    2531

  Fragments arranged by size: 

                3517    2531    1119    1103    1002
 
MslI CAynn'nnrTG

Cuts at:    401    1033    1389    2989    3118    4105    4139    5041    5155 
   Size:        632     356    1600     129     987      34     902     114

Cuts at:   5155    5260    6163    6262    7261    7388     401 
   Size:        105     903      99     999     127    2285

  Fragments arranged by size: 

                2285    1600     999     987     903     902     632     356
                 129     127     114     105      99      34
 
MspA1I CmG'CkG

Cuts at:    256     421     881     920     974    1148    1941    2570    2677 
   Size:        165     460      39      54     174     793     629     107

Cuts at:   2677    2705    3878    5097    5577    5992    6383    6697    7057 
   Size:         28    1173    1219     480     415     391     314     360

Cuts at:   7057    8070    8315     256 
   Size:       1013     245    1213

  Fragments arranged by size: 

                1219    1213    1173    1013     793     629     480     460
                 415     391     360     314     245     174     165     107
                  54      39      28
 
NaeI GCC'GGC

Cuts at:    435     825     898    3815    3921    6051    7453     435 
   Size:        390      73    2917     106    2130    1402    2254

  Fragments arranged by size: 

                2917    2254    2130    1402     390     106      73
 
NarI GG'CG_CC

Cuts at:   1406    6703    6951    1406 
   Size:       5297     248    3727

  Fragments arranged by size: 

                5297    3727     248
 
NcoI C'CATG_G

Cuts at:   3077    4112    3077 
   Size:       1035    8237
 
NgoMIV G'CCGG_C

Cuts at:    433     823     896    3813    3919    6049    7451     433 
   Size:        390      73    2917     106    2130    1402    2254

  Fragments arranged by size: 

                2917    2254    2130    1402     390     106      73
 
NheI G'CTAG_C

Cuts at:   3718    3718 
   Size:       9272
 
NsiI A_TGCA'T

Cuts at:   2116    2116 
   Size:       9272
 
NspI r_CATG'y

Cuts at:   1980    2114    2284    2994    4594    5265    5560    6325    6825 
   Size:        134     170     710    1600     671     295     765     500

Cuts at:   6825    7732    1980 
   Size:        907    3520

  Fragments arranged by size: 

                3520    1600     907     765     710     671     500     295
                 170     134
 
PasI CC'CwG_GG

Cuts at:   5058    5087    5980    5058 
   Size:         29     893    8350

  Fragments arranged by size: 

                8350     893      29
 
PciI A'CATG_T

Cuts at:   5261    7728    5261 
   Size:       2467    6805
 
PflMI CCAn_nnn'nTGG

Cuts at:    834     881    1148    1718    3609     834 
   Size:         47     267     570    1891    6497

  Fragments arranged by size: 

                6497    1891     570     267      47
 
PfoI T'CCnGG_A

Cuts at:    134     791    2792    5296     134 
   Size:        657    2001    2504    4110

  Fragments arranged by size: 

                4110    2504    2001     657
 
PmeI GTTT'AAAC

Cuts at:   6271    6271 
   Size:       9272
 
PmlI CAC'GTG

Cuts at:   4193    4454    4742    4193 
   Size:        261     288    8723

  Fragments arranged by size: 

                8723     288     261
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5323    5355    8411    8443    5323 
   Size:         32    3056      32    6152

  Fragments arranged by size: 

                6152    3056      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1778    3682    4670    5248    1778 
   Size:       1904     988     578    5802

  Fragments arranged by size: 

                5802    1904     988     578
 
PspOMI G'GGCC_C

Cuts at:   1430    3070    3331    3401    5061    5984    6150    1430 
   Size:       1640     261      70    1660     923     166    4552

  Fragments arranged by size: 

                4552    1660    1640     923     261     166      70
 
PstI C_TGCA'G

Cuts at:    356    1687    2072    3152    4610    5582    6319    7004     356 
   Size:       1331     385    1080    1458     972     737     685    2624

  Fragments arranged by size: 

                2624    1458    1331    1080     972     737     685     385
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9272
 
PvuII CAG'CTG

Cuts at:   1941    2705    5097    5577    6697    7057    1941 
   Size:        764    2392     480    1120     360    4156

  Fragments arranged by size: 

                4156    2392    1120     764     480     360
 
RsrII CG'GwC_CG

Cuts at:    962    5745    7467     962 
   Size:       4783    1722    2767

  Fragments arranged by size: 

                4783    2767    1722
 
SacI G_AGCT'C

Cuts at:   2065    3038    6286    2065 
   Size:        973    3248    5051

  Fragments arranged by size: 

                5051    3248     973
 
SacII CC_GC'GG

Cuts at:    257     422    3879     257 
   Size:        165    3457    5650

  Fragments arranged by size: 

                5650    3457     165
 
SalI G'TCGA_C

Cuts at:   6309    6309 
   Size:       9272
 
SanDI GG'GwC_CC

Cuts at:   1778    1778 
   Size:       9272
 
SapI GCTCTTCn'nnn_

Cuts at:   1234    3856    4939    7295    7505    1234 
   Size:       2622    1083    2356     210    3001

  Fragments arranged by size: 

                3001    2622    2356    1083     210
 
SbfI CC_TGCA'GG

Cuts at:   4610    6319    4610 
   Size:       1709    7563
 
ScaI AGT'ACT

Cuts at:   4366    8789    4366 
   Size:       4423    4849
 
SfcI C'TryA_G

Cuts at:     33     352    1683    2068    3148    3183    3594    4360    4606 
   Size:        319    1331     385    1080      35     411     766     246

Cuts at:   4606    5454    5578    6315    7000    7993    8184      33 
   Size:        848     124     737     685     993     191    1121

  Fragments arranged by size: 

                1331    1121    1080     993     848     766     737     685
                 411     385     319     246     191     124      35
 
SfoI GGC'GCC

Cuts at:   1407    6704    6952    1407 
   Size:       5297     248    3727

  Fragments arranged by size: 

                5297    3727     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9272
 
SmaI CCC'GGG

Cuts at:   1371    3801    5545    6294    1371 
   Size:       2430    1744     749    4349

  Fragments arranged by size: 

                4349    2430    1744     749
 
SmlI C'TyrA_G

Cuts at:    886    1315    1783    2089    2668    3274    4441    4853    5340 
   Size:        429     468     306     579     606    1167     412     487

Cuts at:   5340    6190    6427    7342    7834    8096     886 
   Size:        850     237     915     492     262    2062

  Fragments arranged by size: 

                2062    1167     915     850     606     579     492     487
                 468     429     412     306     262     237
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9272
 
SphI G_CATG'C

Cuts at:   2284    4594    5560    6325    6825    2284 
   Size:       2310     966     765     500    4731

  Fragments arranged by size: 

                4731    2310     966     765     500
 
SrfI GCCC'GGGC

Cuts at:   5545    5545 
   Size:       9272
 
StuI AGG'CCT

Cuts at:   5371    5833    5890    5371 
   Size:        462      57    8753

  Fragments arranged by size: 

                8753     462      57
 
StyI C'CwwG_G

Cuts at:   1400    2264    2285    2630    3077    3665    4012    4079    4112 
   Size:        864      21     345     447     588     347      67      33

Cuts at:   4112    4664    5963    6406    6760    1400 
   Size:        552    1299     443     354    3912

  Fragments arranged by size: 

                3912    1299     864     588     552     447     443     354
                 347     345      67      33      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    979    1638    2277    3150    3634    3888    4588    4854    5240 
   Size:        659     639     873     484     254     700     266     386

Cuts at:   5240    5742    5938    5993    6108    6855    6988    7447    7656 
   Size:        502     196      55     115     747     133     459     209

Cuts at:   7656     979 
   Size:       2595

  Fragments arranged by size: 

                2595     873     747     700     659     639     502     484
                 459     386     266     254     209     196     133     115
                  55
 
TatI w'GTAC_w

Cuts at:    653    1481    2978    2993    3215    3776    4364    5203    8787 
   Size:        828    1497      15     222     561     588     839    3584

Cuts at:   8787     653 
   Size:       1138

  Fragments arranged by size: 

                3584    1497    1138     839     828     588     561     222
                  15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1613    1645    1813    1845    3098    3130    4308    4340    4567 
   Size:         32     168      32    1253      32    1178      32     227

Cuts at:   4567    4599    6120    6152    8789    8821    1613 
   Size:         32    1521      32    2637      32    2064

  Fragments arranged by size: 

                2637    2064    1521    1253    1178     227     168      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    601    2587    2605    2647    7069     601 
   Size:       1986      18      42    4422    2804

  Fragments arranged by size: 

                4422    2804    1986      42      18
 
XbaI T'CTAG_A

Cuts at:   6303    6303 
   Size:       9272
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4485    4920    5901    5961    6767    4485 
   Size:        435     981      60     806    6990

  Fragments arranged by size: 

                6990     981     806     435      60
 
XhoI C'TCGA_G

Cuts at:   6190    6190 
   Size:       9272
 
XmaI C'CCGG_G

Cuts at:   1369    3799    5543    6292    1369 
   Size:       2430    1744     749    4349

  Fragments arranged by size: 

                4349    2430    1744     749
 
XmnI GAAnn'nnTTC

Cuts at:   4882    4882 
   Size:       9272
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AscI    AvrII     BaeI     BsmI   BssHII
  BstZ17I   Bsu36I     ClaI    EcoRV    FspAI     HpaI     MfeI     NdeI
     NotI     NruI     PacI    PshAI     PsiI    PspXI     PsrI    SexAI
     SfiI    SgrAI    SnaBI     SspI     SwaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com