Restriction map for pF1KA1124
 (Linear) (Six-base) MAPSORT of: KIAA1124.seq  Check: 2959  from: 1  to: 5133



KIAA1124.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4033    4794    5133 
   Size:       4033     761     339

  Fragments arranged by size: 

                4033     761     339
 
AatII G_ACGT'C

Cuts at:      0    4457    5133 
   Size:       4457     676
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     950    1264    3416    4305    4544    5133 
   Size:        950     314    2152     889     239     589

  Fragments arranged by size: 

                2152     950     889     589     314     239
 
AflIII A'CryG_T

Cuts at:      0    2615    3191    5133 
   Size:       2615     576    1942

  Fragments arranged by size: 

                2615    1942     576
 
AhdI GACnn_n'nnGTC

Cuts at:      0     792    5133 
   Size:        792    4341
 
AleI CACnn'nnGTG

Cuts at:      0    3325    5133 
   Size:       3325    1808
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1396    4822    5133 
   Size:       1396    3426     311

  Fragments arranged by size: 

                3426    1396     311
 
AlwNI CAG_nnn'CTG

Cuts at:      0     957    1581    2409    2610    2818    3045    3122    4134 
   Size:        957     624     828     201     208     227      77    1012

Cuts at:   4134    4157    4641    5133 
   Size:         23     484     492

  Fragments arranged by size: 

                1012     957     828     624     492     484     227     208
                 201      77      23
 
ApaI G_GGCC'C

Cuts at:      0      53    1431    4789    4864    5133 
   Size:         53    1378    3358      75     269

  Fragments arranged by size: 

                3358    1378     269      75      53
 
ApaLI G'TGCA_C

Cuts at:      0     589    3551    3907    5133 
   Size:        589    2962     356    1226

  Fragments arranged by size: 

                2962    1226     589     356
 
ApoI r'AATT_y

Cuts at:      0     299     499    2777    2803    5133 
   Size:        299     200    2278      26    2330

  Fragments arranged by size: 

                2330    2278     299     200      26
 
AvaI C'yCGr_G

Cuts at:      0     166    1424    1519    2492    2536    3235    3803    4587 
   Size:        166    1258      95     973      44     699     568     784

Cuts at:   4587    5133 
   Size:        546

  Fragments arranged by size: 

                1258     973     784     699     568     546     166      95
                  44
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     908     941    5133 
   Size:        908      33    4192

  Fragments arranged by size: 

                4192     908      33
 
BanI G'GyrC_C

Cuts at:      0    2020    3208    4021    4528    5133 
   Size:       2020    1188     813     507     605

  Fragments arranged by size: 

                2020    1188     813     605     507
 
BanII G_rGCy'C

Cuts at:      0      53    1431    1760    2537    2690    2825    3472    3785 
   Size:         53    1378     329     777     153     135     647     313

Cuts at:   3785    4295    4379    4789    4864    5071    5133 
   Size:        510      84     410      75     207      62

  Fragments arranged by size: 

                1378     777     647     510     410     329     313     207
                 153     135      84      75      62      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     227     512    4662    5133 
   Size:        227     285    4150     471

  Fragments arranged by size: 

                4150     471     285     227
 
BbvCI CC'TCA_GC

Cuts at:      0    1432    1913    3661    5133 
   Size:       1432     481    1748    1472

  Fragments arranged by size: 

                1748    1472    1432     481
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     196     230    2470    2504    5133 
   Size:        196      34    2240      34    2629

  Fragments arranged by size: 

                2629    2240     196      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2650    5133 
   Size:       2650    2483
 
BlpI GC'TnA_GC

Cuts at:      0     493    1369    1892    2907    4189    5133 
   Size:        493     876     523    1015    1282     944

  Fragments arranged by size: 

                1282    1015     944     876     523     493
 
Bme1580I G_kGCm'C

Cuts at:      0      53     593     715    1431    1767    3213    3226    3555 
   Size:         53     540     122     716     336    1446      13     329

Cuts at:   3555    3911    4365    4789    4838    4864    5133 
   Size:        356     454     424      49      26     269

  Fragments arranged by size: 

                1446     716     540     454     424     356     336     329
                 269     122      53      49      26      13
 
BmgBI CAC'GTC

Cuts at:      0      96     627    1113    1125    2110    3267    4425    5133 
   Size:         96     531     486      12     985    1157    1158     708

  Fragments arranged by size: 

                1158    1157     985     708     531     486      96      12
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2448    2828    5133 
   Size:       2448     380    2305

  Fragments arranged by size: 

                2448    2305     380
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4615    5133 
   Size:       4615     518
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49    1321    1384    1612    2002    2089    2410    2650 
   Size:         49    1272      63     228     390      87     321     240

Cuts at:   2650    2668    3084    3619    5131    5133 
   Size:         18     416     535    1512       2

  Fragments arranged by size: 

                1512    1272     535     416     390     321     240     228
                  87      63      49      18       2
 
Bpu10I CC'TnA_GC

Cuts at:      0      73    1432    1873    1913    2689    3661    4144    5133 
   Size:         73    1359     441      40     776     972     483     989

  Fragments arranged by size: 

                1359     989     972     776     483     441      73      40
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       4    1552    1975    3991    5133 
   Size:          4    1548     423    2016    1142

  Fragments arranged by size: 

                2016    1548    1142     423       4
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     343     800     856    4715    5133 
   Size:        343     457      56    3859     418

  Fragments arranged by size: 

                3859     457     418     343      56
 
BsaAI yAC'GTr

Cuts at:      0     153     588    3192    4341    4773    5133 
   Size:        153     435    2604    1149     432     360

  Fragments arranged by size: 

                2604    1149     435     432     360     153
 
BsaBI GATnn'nnATC

Cuts at:      0    1697    3877    5133 
   Size:       1697    2180    1256

  Fragments arranged by size: 

                2180    1697    1256
 
BsaHI Gr'CG_yC

Cuts at:      0    1838    2705    4454    5133 
   Size:       1838     867    1749     679

  Fragments arranged by size: 

                1838    1749     867     679
 
BsaWI w'CCGG_w

Cuts at:      0     516    3966    5133 
   Size:        516    3450    1167

  Fragments arranged by size: 

                3450    1167     516
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2541    2571    2623    2653    2916    2946    3112    3142 
   Size:       2541      30      52      30     263      30     166      30

Cuts at:   3142    4366    4396    5133 
   Size:       1224      30     737

  Fragments arranged by size: 

                2541    1224     737     263     166      52      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     382    1347    1642    1819    1822    2269    2562    2698 
   Size:        382     965     295     177       3     447     293     136

Cuts at:   2698    2864    3874    4318    4531    5133 
   Size:        166    1010     444     213     602

  Fragments arranged by size: 

                1010     965     602     447     444     382     295     293
                 213     177     166     136       3
 
BseYI C'CCAG_C

Cuts at:      0     981    3410    5053    5133 
   Size:        981    2429    1643      80

  Fragments arranged by size: 

                2429    1643     981      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     400     712    1309    1423    1618    3108    3135 
   Size:        141     259     312     597     114     195    1490      27

Cuts at:   3135    3289    4147    4504    5133 
   Size:        154     858     357     629

  Fragments arranged by size: 

                1490     858     629     597     357     312     259     195
                 154     141     114      27
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     593    1307    1760    1946    2036    2537    2690 
   Size:        101     492     714     453     186      90     501     153

Cuts at:   2690    3472    3555    3911    4145    4295    4379    4811    5133 
   Size:        782      83     356     234     150      84     432     322

  Fragments arranged by size: 

                 782     714     501     492     453     432     356     322
                 234     186     153     150     101      90      84      83
            
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     101     593     715    1307    1431    1760    1767 
   Size:         53      48     492     122     592     124     329       7

Cuts at:   1767    1946    2036    2537    2690    2825    3213    3226    3472 
   Size:        179      90     501     153     135     388      13     246

Cuts at:   3472    3555    3785    3911    4145    4295    4365    4379    4789 
   Size:         83     230     126     234     150      70      14     410

Cuts at:   4789    4811    4838    4864    5071    5133 
   Size:         22      27      26     207      62

  Fragments arranged by size: 

                 592     501     492     410     388     329     246     234
                 230     207     179     153     150     135     126     124
                 122      90      83      70      62      53      48      27
                  26      22      14      13       7
 
BspEI T'CCGG_A

Cuts at:      0     516    3966    5133 
   Size:        516    3450    1167

  Fragments arranged by size: 

                3450    1167     516
 
BspHI T'CATG_A

Cuts at:      0     878    5133 
   Size:        878    4255
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4033    4319    4794    4826    5133 
   Size:       4033     286     475      32     307

  Fragments arranged by size: 

                4033     475     307     286      32
 
BsrBI CCG'CTC

Cuts at:      0    1662    2017    3940    4594    4867    5133 
   Size:       1662     355    1923     654     273     266

  Fragments arranged by size: 

                1923    1662     654     355     273     266
 
BsrDI GCAATG_nn'

Cuts at:      0     314    3422    5133 
   Size:        314    3108    1711

  Fragments arranged by size: 

                3108    1711     314
 
BsrFI r'CCGG_y

Cuts at:      0     977    3656    5118    5133 
   Size:        977    2679    1462      15

  Fragments arranged by size: 

                2679    1462     977      15
 
BsrGI T'GTAC_A

Cuts at:      0     110    4433    5133 
   Size:        110    4323     700

  Fragments arranged by size: 

                4323     700     110
 
BssHII G'CGCG_C

Cuts at:      0    3345    4367    5133 
   Size:       3345    1022     766

  Fragments arranged by size: 

                3345    1022     766
 
BssSI C'ACGA_G

Cuts at:      0     856    4917    5133 
   Size:        856    4061     216

  Fragments arranged by size: 

                4061     856     216
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1311    1581    1854    3122    4641    5133 
   Size:       1311     270     273    1268    1519     492

  Fragments arranged by size: 

                1519    1311    1268     492     273     270
 
BstEII G'GTnAC_C

Cuts at:      0    2497    3798    5133 
   Size:       2497    1301    1335

  Fragments arranged by size: 

                2497    1335    1301
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4878    5133 
   Size:       4878     255
 
BstYI r'GATC_y

Cuts at:      0     737    2406    2657    3441    4067    4735    5133 
   Size:        737    1669     251     784     626     668     398

  Fragments arranged by size: 

                1669     784     737     668     626     398     251
 
BtgI C'CryG_G

Cuts at:      0     701     707    1413    3320    3919    3995    4472    5133 
   Size:        701       6     706    1907     599      76     477     661

  Fragments arranged by size: 

                1907     706     701     661     599     477      76       6
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     389     763    2314    5133 
   Size:        389     374    1551    2819

  Fragments arranged by size: 

                2819    1551     389     374
 
BtsI GCAGTG_nn'

Cuts at:      0    2867    3120    4135    5133 
   Size:       2867     253    1015     998

  Fragments arranged by size: 

                2867    1015     998     253
 
DraIII CAC_nnn'GTG

Cuts at:      0     119    1608    3906    5133 
   Size:        119    1489    2298    1227

  Fragments arranged by size: 

                2298    1489    1227     119
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     100    1404    5133 
   Size:        100    1304    3729

  Fragments arranged by size: 

                3729    1304     100
 
EaeI y'GGCC_r

Cuts at:      0       5     155     557     704    1820    3891    3992    3998 
   Size:          5     150     402     147    1116    2071     101       6

Cuts at:   3998    4037    5133 
   Size:         39    1096

  Fragments arranged by size: 

                2071    1116    1096     402     150     147     101      39
                   6       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     243     884    1240    2087    2525    3382    4682    5133 
   Size:        243     641     356     847     438     857    1300     451

  Fragments arranged by size: 

                1300     857     847     641     451     438     356     243
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     631    2668    5133 
   Size:        631    2037    2465

  Fragments arranged by size: 

                2465    2037     631
 
EcoICRI GAG'CTC

Cuts at:      0    1758    2535    2688    3470    4293    4377    5133 
   Size:       1758     777     153     782     823      84     756

  Fragments arranged by size: 

                1758     823     782     777     756     153      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     950    1264    1321    1384    1612    2002    2089 
   Size:         49     901     314      57      63     228     390      87

Cuts at:   2089    2410    2650    2668    3084    3416    3619    4305    4544 
   Size:        321     240      18     416     332     203     686     239

Cuts at:   4544    5131    5133 
   Size:        587       2

  Fragments arranged by size: 

                 901     686     587     416     390     332     321     314
                 240     239     228     203      87      63      57      49
                  18       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50     942    1296    1428    1986    3042    3309    4511 
   Size:         50     892     354     132     558    1056     267    1202

Cuts at:   4511    4677    4861    5133 
   Size:        166     184     272

  Fragments arranged by size: 

                1202    1056     892     558     354     272     267     184
                 166     132      50
 
EcoRV GAT'ATC

Cuts at:      0    4748    5133 
   Size:       4748     385
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     272     529    1912    2097    5133 
   Size:        272     257    1383     185    3036

  Fragments arranged by size: 

                3036    1383     272     257     185
 
FspI TGC'GCA

Cuts at:      0    4468    4645    5133 
   Size:       4468     177     488

  Fragments arranged by size: 

                4468     488     177
 
FspAI rTGC'GCAy

Cuts at:      0    4468    5133 
   Size:       4468     665
 
HaeII r_GCGC'y

Cuts at:      0      71    1549    5133 
   Size:         71    1478    3584

  Fragments arranged by size: 

                3584    1478      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     643     675     712     744     778     810    1222    1254 
   Size:        643      32      37      32      34      32     412      32

Cuts at:   1254    2541    2573    2623    2655    3525    3557    3797    3829 
   Size:       1287      32      50      32     870      32     240      32

Cuts at:   3829    4098    4130    4944    4976    4999    5031    5133 
   Size:        269      32     814      32      23      32     102

  Fragments arranged by size: 

                1287     870     814     643     412     269     240     102
                  50      37      34      32      32      32      32      32
                  32      32      32      32      32      32      23
 
HindIII A'AGCT_T

Cuts at:      0     511    1322    1933    3961    5133 
   Size:        511     811     611    2028    1172

  Fragments arranged by size: 

                2028    1172     811     611     511
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1127    1286    4643    4775    5072    5133 
   Size:       1127     159    3357     132     297      61

  Fragments arranged by size: 

                3357    1127     297     159     132      61
 
MscI TGG'CCA

Cuts at:      0     559    1822    3994    5133 
   Size:        559    1263    2172    1139

  Fragments arranged by size: 

                2172    1263    1139     559
 
MslI CAynn'nnrTG

Cuts at:      0    3325    4804    5133 
   Size:       3325    1479     329

  Fragments arranged by size: 

                3325    1479     329
 
MspA1I CmG'CkG

Cuts at:      0      36     192    1293    1578    1791    2178    2388    2583 
   Size:         36     156    1101     285     213     387     210     195

Cuts at:   2583    3019    3736    3826    5057    5133 
   Size:        436     717      90    1231      76

  Fragments arranged by size: 

                1231    1101     717     436     387     285     213     210
                 195     156      90      76      36
 
NaeI GCC'GGC

Cuts at:      0     979    5120    5133 
   Size:        979    4141      13

  Fragments arranged by size: 

                4141     979      13
 
NcoI C'CATG_G

Cuts at:      0    4472    5133 
   Size:       4472     661
 
NdeI CA'TA_TG

Cuts at:      0    3351    5133 
   Size:       3351    1782
 
NgoMIV G'CCGG_C

Cuts at:      0     977    5118    5133 
   Size:        977    4141      15

  Fragments arranged by size: 

                4141     977      15
 
NspI r_CATG'y

Cuts at:      0     813    1018    2619    3682    4803    5133 
   Size:        813     205    1601    1063    1121     330

  Fragments arranged by size: 

                1601    1121    1063     813     330     205
 
PasI CC'CwG_GG

Cuts at:      0    3863    5133 
   Size:       3863    1270
 
PciI A'CATG_T

Cuts at:      0    2615    5133 
   Size:       2615    2518
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1195    2178    2610    3438    3990    4478    5133 
   Size:       1195     983     432     828     552     488     655

  Fragments arranged by size: 

                1195     983     828     655     552     488     432
 
PfoI T'CCnGG_A

Cuts at:      0      41    2678    4897    5133 
   Size:         41    2637    2219     236

  Fragments arranged by size: 

                2637    2219     236      41
 
PmlI CAC'GTG

Cuts at:      0    3192    4773    5133 
   Size:       3192    1581     360

  Fragments arranged by size: 

                3192    1581     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     942    1296    3309    4677    5133 
   Size:        942     354    2013    1368     456

  Fragments arranged by size: 

                2013    1368     942     456     354
 
PshAI GACnn'nnGTC

Cuts at:      0    3636    5133 
   Size:       3636    1497
 
PspOMI G'GGCC_C

Cuts at:      0      49    1427    4785    4860    5133 
   Size:         49    1378    3358      75     273

  Fragments arranged by size: 

                3358    1378     273      75      49
 
PspXI vC'TCGA_Gb

Cuts at:      0     166    1519    2536    5133 
   Size:        166    1353    1017    2597

  Fragments arranged by size: 

                2597    1353    1017     166
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4894    4926    5133 
   Size:       4894      32     207

  Fragments arranged by size: 

                4894     207      32
 
PstI C_TGCA'G

Cuts at:      0     746     967    1316    1724    2189    2405    2639    4700 
   Size:        746     221     349     408     465     216     234    2061

Cuts at:   4700    5133 
   Size:        433

  Fragments arranged by size: 

                2061     746     465     433     408     349     234     221
                 216
 
PvuII CAG'CTG

Cuts at:      0      36     192    1791    2178    2388    3736    5133 
   Size:         36     156    1599     387     210    1348    1397

  Fragments arranged by size: 

                1599    1397    1348     387     210     156      36
 
SacI G_AGCT'C

Cuts at:      0    1760    2537    2690    3472    4295    4379    5133 
   Size:       1760     777     153     782     823      84     754

  Fragments arranged by size: 

                1760     823     782     777     754     153      84
 
SapI GCTCTTCn'nnn_

Cuts at:      0     884    1240    2525    3382    5133 
   Size:        884     356    1285     857    1751

  Fragments arranged by size: 

                1751    1285     884     857     356
 
SbfI CC_TGCA'GG

Cuts at:      0     746    1724    5133 
   Size:        746     978    3409

  Fragments arranged by size: 

                3409     978     746
 
SexAI A'CCwGG_T

Cuts at:      0    4220    5133 
   Size:       4220     913
 
SfcI C'TryA_G

Cuts at:      0     742     963    1312    1720    2185    2401    2635    4404 
   Size:        742     221     349     408     465     216     234    1769

Cuts at:   4404    4696    5133 
   Size:        292     437

  Fragments arranged by size: 

                1769     742     465     437     408     349     292     234
                 221     216
 
SmaI CCC'GGG

Cuts at:      0    2494    5133 
   Size:       2494    2639
 
SmlI C'TyrA_G

Cuts at:      0      19     166    1519    1531    1990    2536    4006    5133 
   Size:         19     147    1353      12     459     546    1470    1127

  Fragments arranged by size: 

                1470    1353    1127     546     459     147      19      12
            
 
SphI G_CATG'C

Cuts at:      0    1018    4803    5133 
   Size:       1018    3785     330

  Fragments arranged by size: 

                3785    1018     330
 
SspI AAT'ATT

Cuts at:      0    1145    5133 
   Size:       1145    3988
 
StuI AGG'CCT

Cuts at:      0    1918    2116    3252    5108    5133 
   Size:       1918     198    1136    1856      25

  Fragments arranged by size: 

                1918    1856    1136     198      25
 
StyI C'CwwG_G

Cuts at:      0       8    1679    1922    2717    3686    4472    5133 
   Size:          8    1671     243     795     969     786     661

  Fragments arranged by size: 

                1671     969     795     786     661     243       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     460     790    1408    2542    3075    3424    4435    4470 
   Size:        460     330     618    1134     533     349    1011      35

Cuts at:   4470    5133 
   Size:        663

  Fragments arranged by size: 

                1134    1011     663     618     533     460     349     330
                  35
 
TatI w'GTAC_w

Cuts at:      0     110    4433    5133 
   Size:        110    4323     700

  Fragments arranged by size: 

                4323     700     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2569    2601    3112    3144    4325    4357    4366    4398 
   Size:       2569      32     511      32    1181      32       9      32

Cuts at:   4398    5133 
   Size:        735

  Fragments arranged by size: 

                2569    1181     735     511      32      32      32      32
                   9
 
Tth111I GACn'n_nGTC

Cuts at:      0     613    5133 
   Size:        613    4520
 
XbaI T'CTAG_A

Cuts at:      0    3624    5133 
   Size:       3624    1509
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1110    1830    2715    3042    3252    5133 
   Size:       1110     720     885     327     210    1881

  Fragments arranged by size: 

                1881    1110     885     720     327     210
 
XhoI C'TCGA_G

Cuts at:      0     166    1519    2536    5133 
   Size:        166    1353    1017    2597

  Fragments arranged by size: 

                2597    1353    1017     166
 
XmaI C'CCGG_G

Cuts at:      0    2492    5133 
   Size:       2492    2641
 
XmnI GAAnn'nnTTC

Cuts at:      0    2462    4084    5133 
   Size:       2462    1622    1049

  Fragments arranged by size: 

                2462    1622    1049
 
ZraI GAC'GTC

Cuts at:      0    4455    5133 
   Size:       4455     678
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI
     FspI    FspAI    HaeII    Hin4I  HindIII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI     SapI     SbfI    SexAI     SfcI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AloI     AscI
     AseI    AsiSI    AvrII    BamHI     BbeI    BciVI     BclI    BfrBI
    BglII     BmtI    BsiEI    BsiWI     BsmI    BsmBI    BstBI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     EagI    EcoNI    EcoRI     FseI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PpiI     PsiI     PvuI
    RsrII    SacII     SalI    SanDI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI
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