Restriction map for pF1KA1101
 (Linear) (Six-base) MAPSORT of: KIAA1101.seq  Check: 3488  from: 1  to: 1581



KIAA1101.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AccI GT'mk_AC

Cuts at:      0    1421    1581 
   Size:       1421     160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1250    1581 
   Size:        605     645     331

  Fragments arranged by size: 

                 645     605     331
 
AleI CACnn'nnGTG

Cuts at:      0     341    1581 
   Size:        341    1240
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     281     313    1581 
   Size:        281      32    1268

  Fragments arranged by size: 

                1268     281      32
 
ApoI r'AATT_y

Cuts at:      0     188     865     892    1024    1101    1323    1357    1581 
   Size:        188     677      27     132      77     222      34     224

  Fragments arranged by size: 

                 677     224     222     188     132      77      34      27
            
 
AvaI C'yCGr_G

Cuts at:      0      12    1079    1581 
   Size:         12    1067     502

  Fragments arranged by size: 

                1067     502      12
 
BanI G'GyrC_C

Cuts at:      0     562     579    1581 
   Size:        562      17    1002

  Fragments arranged by size: 

                1002     562      17
 
BanII G_rGCy'C

Cuts at:      0    1112    1581 
   Size:       1112     469
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     973    1256    1581 
   Size:        973     283     325

  Fragments arranged by size: 

                 973     325     283
 
BbvCI CC'TCA_GC

Cuts at:      0    1178    1581 
   Size:       1178     403
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       3      37    1581 
   Size:          3      34    1544

  Fragments arranged by size: 

                1544      34       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1188    1581 
   Size:       1188     393
 
BglII A'GATC_T

Cuts at:      0    1160    1581 
   Size:       1160     421
 
BlpI GC'TnA_GC

Cuts at:      0    1570    1581 
   Size:       1570      11
 
Bme1580I G_kGCm'C

Cuts at:      0     111    1581 
   Size:        111    1470
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1407    1581 
   Size:       1407     174
 
Bpu10I CC'TnA_GC

Cuts at:      0    1178    1253    1581 
   Size:       1178      75     328

  Fragments arranged by size: 

                1178     328      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169    1196    1581 
   Size:        169    1027     385

  Fragments arranged by size: 

                1027     385     169
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1079    1581 
   Size:       1079     502
 
BsaBI GATnn'nnATC

Cuts at:      0    1297    1581 
   Size:       1297     284
 
BsaHI Gr'CG_yC

Cuts at:      0     978    1581 
   Size:        978     603
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     281     311    1581 
   Size:        281      30    1270

  Fragments arranged by size: 

                1270     281      30
 
BseYI C'CCAG_C

Cuts at:      0    1004    1186    1565    1581 
   Size:       1004     182     379      16

  Fragments arranged by size: 

                1004     379     182      16
 
BsiHKAI G_wGCw'C

Cuts at:      0     924    1112    1581 
   Size:        924     188     469

  Fragments arranged by size: 

                 924     469     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     924    1112    1581 
   Size:        111     813     188     469

  Fragments arranged by size: 

                 813     469     188     111
 
BspHI T'CATG_A

Cuts at:      0     272    1581 
   Size:        272    1309
 
BsrDI GCAATG_nn'

Cuts at:      0     365     910    1581 
   Size:        365     545     671

  Fragments arranged by size: 

                 671     545     365
 
BstYI r'GATC_y

Cuts at:      0     425     822    1160    1292    1581 
   Size:        425     397     338     132     289

  Fragments arranged by size: 

                 425     397     338     289     132
 
BtgI C'CryG_G

Cuts at:      0     603    1581 
   Size:        603     978
 
BtsI GCAGTG_nn'

Cuts at:      0     715    1581 
   Size:        715     866
 
ClaI AT'CG_AT

Cuts at:      0    1488    1581 
   Size:       1488      93
 
DraIII CAC_nnn'GTG

Cuts at:      0     321    1581 
   Size:        321    1260
 
EcoICRI GAG'CTC

Cuts at:      0    1110    1581 
   Size:       1110     471
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1250    1581 
   Size:        605     645     331

  Fragments arranged by size: 

                 645     605     331
 
EcoRI G'AATT_C

Cuts at:      0    1323    1581 
   Size:       1323     258
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     913    1581 
   Size:        913     668
 
HaeII r_GCGC'y

Cuts at:      0      20    1581 
   Size:         20    1561
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1152    1184    1581 
   Size:       1152      32     397

  Fragments arranged by size: 

                1152     397      32
 
HincII GTy'rAC

Cuts at:      0    1422    1581 
   Size:       1422     159
 
MfeI C'AATT_G

Cuts at:      0     650    1581 
   Size:        650     931
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     553    1141    1581 
   Size:        553     588     440

  Fragments arranged by size: 

                 588     553     440
 
MslI CAynn'nnrTG

Cuts at:      0     341     416     434    1581 
   Size:        341      75      18    1147

  Fragments arranged by size: 

                1147     341      75      18
 
MspA1I CmG'CkG

Cuts at:      0    1186    1581 
   Size:       1186     395
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     281     313    1581 
   Size:        281      32    1268

  Fragments arranged by size: 

                1268     281      32
 
PspXI vC'TCGA_Gb

Cuts at:      0      12    1581 
   Size:         12    1569
 
PstI C_TGCA'G

Cuts at:      0      59     722    1581 
   Size:         59     663     859

  Fragments arranged by size: 

                 859     663      59
 
PvuII CAG'CTG

Cuts at:      0    1186    1581 
   Size:       1186     395
 
SacI G_AGCT'C

Cuts at:      0    1112    1581 
   Size:       1112     469
 
SalI G'TCGA_C

Cuts at:      0    1420    1581 
   Size:       1420     161
 
ScaI AGT'ACT

Cuts at:      0     389    1310    1581 
   Size:        389     921     271

  Fragments arranged by size: 

                 921     389     271
 
SexAI A'CCwGG_T

Cuts at:      0     963    1581 
   Size:        963     618
 
SfcI C'TryA_G

Cuts at:      0      55      86     662     718    1581 
   Size:         55      31     576      56     863

  Fragments arranged by size: 

                 863     576      56      55      31
 
SmlI C'TyrA_G

Cuts at:      0      12     148    1211    1581 
   Size:         12     136    1063     370

  Fragments arranged by size: 

                1063     370     136      12
 
SspI AAT'ATT

Cuts at:      0     222    1581 
   Size:        222    1359
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1242    1581 
   Size:       1242     339
 
TatI w'GTAC_w

Cuts at:      0     387     477    1308    1581 
   Size:        387      90     831     273

  Fragments arranged by size: 

                 831     387     273      90
 
XhoI C'TCGA_G

Cuts at:      0      12    1581 
   Size:         12    1569
 
XmnI GAAnn'nnTTC

Cuts at:      0     896    1581 
   Size:        896     685
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AloI     ApoI     AvaI     BanI    BanII
     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BsrDI    BstYI     BtgI     BtsI     ClaI   DraIII
  EcoICRI  Eco57MI    EcoRI     FalI    HaeII    Hin4I   HincII     MfeI
     MmeI     MslI   MspA1I     PpiI    PspXI     PstI    PvuII     SacI
     SalI     ScaI    SexAI     SfcI     SmlI     SspI    TaqII     TatI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI    BmgBI
     BmrI     BmtI     BpmI    BsaAI    BsaWI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XmaI     ZraI
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