Restriction map for pF1KA1071
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BbsI        BciVI       BglII       BpuAI       BsaAI       
BsaBI       BsiEI       BsiWI       BspDI       BspHI       
BspLU11I    BsrBI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       FauI        FseI        FspI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII        1411  CGCG                 CG^CG
AccIII       1880  TCCGGA               T^CCGGA
AclI         1951  AACGTT               AA^CGTT
AcyI          434  GACGCC               G[GA]^CG[CT]C
AflII         496  CTTAAG               C^TTAAG
AgeI         3097  ACCGGT               A^CCGGT
ApaI          165  GGGCCC               GGGCC^C
BanI         3006  GGCACC               G^G[CT][GA]CC
BcgI         2540  CGACTCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2282  TGATCA               T^GATCA
BfrI          496  CTTAAG               C^TTAAG
BglI          686  GCCTGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1237  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1237  GCTCAGC              GC^T[ACGT]AGC
BsaHI         434  GACGCC               G[GA]^CG[CT]C
BseAI        1880  TCCGGA               T^CCGGA
BsgI         1973  CTGCAC               GTGCAG(16/14)
Bsp1407I     1830  TGTACA               T^GTACA
BspEI        1880  TCCGGA               T^CCGGA
BsrDI         890  CATTGC               GCAATG(2/0)
BsrFI        3097  ACCGGT               [GA]^CCGG[CT]
BsrGI        1830  TGTACA               T^GTACA
BssSI        1435  CTCGTG               CACGAG(-5/-1)
BstUI        1411  CGCG                 CG^CG
CelII        1237  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3097  ACCGGT               [GA]^CCGG[CT]
DrdI         2119  GACACAACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1771  GAAGAG               CTCTTC(1/4)
EcoRI         646  GAATTC               G^AATTC
EcoT22I      1510  ATGCAT               ATGCA^T
FbaI         2282  TGATCA               T^GATCA
Hin1I         434  GACGCC               G[GA]^CG[CT]C
HincII       1798  GTTGAC               GT[CT]^[GA]AC
HindII       1798  GTTGAC               GT[CT]^[GA]AC
Ksp632I      1771  GAAGAG               CTCTTC(1/4)
MroI         1880  TCCGGA               T^CCGGA
MvnI         1411  CGCG                 CG^CG
NcoI         2717  CCATGG               C^CATGG
NdeI         1424  CATATG               CA^TATG
NsiI         1510  ATGCAT               ATGCA^T
PflMI        1302  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        3097  ACCGGT               A^CCGGT
PpuMI         952  GGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I     1951  AACGTT               AA^CGTT
SacI         1984  GAGCTC               GAGCT^C
SapI         1771  GAAGAGC              GCTCTTC(1/4)
SmaI          162  CCCGGG               CCC^GGG
SspBI        1830  TGTACA               T^GTACA
SspI         2051  AATATT               AAT^ATT
Van91I       1302  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         162  CCCGGG               C^CCGGG
XmaI          162  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI            6  AAATTC               [GA]^AATT[CT]
              646  GAATTC              
ApoI            6  AAATTC               [GA]^AATT[CT]
              646  GAATTC              
AvaI          162  CCCGGG               C^[CT]CG[GA]G
             1232  CTCGGG              
AvrII        1048  CCTAGG               C^CTAGG
             2469  CCTAGG              
BbvCI        2861  CCTCAGC              CCTCAGC(-5/-2)
             3073  GCTGAGG             
BlnI         1048  CCTAGG               C^CTAGG
             2469  CCTAGG              
BsaI          491  GAGACC               GGTCTC(1/5)
             1028  GAGACC              
BsmI          296  GAATGC               GAATGC(1/-1)
             2474  GCATTC              
BsoBI         162  CCCGGG               C^[CT]CG[GA]G
             1232  CTCGGG              
BspMI        1043  ACCTGC               ACCTGC(4/8)
             3136  ACCTGC              
BstAPI         45  GCAGCGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2626  GCAGCTGTTGC         
BstXI        2711  CCACCACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3176  CCAATACTCTGG        
CfoI          877  GCGC                 GCG^C
             1410  GCGC                
DsaI           35  CCACGG               C^C[GA][CT]GG
             2717  CCATGG              
HhaI          877  GCGC                 GCG^C
             1410  GCGC                
HinP1I        877  GCGC                 G^CGC
             1410  GCGC                
ScaI          350  AGTACT               AGT^ACT
             2289  AGTACT              
Tsp509I         7  AATT                 ^AATT
              647  AATT                
XbaI         1542  TCTAGA               T^CTAGA
             2166  TCTAGA              
XcmI         2592  CCAAACTGAACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3200  CCATTCAGATCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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