Restriction map for pF1KA1071
 (Linear) (Six-base) MAPSORT of: KIAA1071.seq  Check: 3379  from: 1  to: 3252



KIAA1071.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1052    3252 
   Size:       1052    2200
 
AclI AA'CG_TT

Cuts at:      0    1952    3252 
   Size:       1952    1300
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     709    2999    3252 
   Size:         32     677    2290     253

  Fragments arranged by size: 

                2290     677     253      32
 
AflII C'TTAA_G

Cuts at:      0     496    3252 
   Size:        496    2756
 
AgeI A'CCGG_T

Cuts at:      0    3097    3252 
   Size:       3097     155
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1637    3252 
   Size:       1637    1615
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1734    2662    2740    2767    2791    2827    2848    2869 
   Size:       1734     928      78      27      24      36      21      21

Cuts at:   2869    3210    3252 
   Size:        341      42

  Fragments arranged by size: 

                1734     928     341      78      42      36      27      24
                  21      21
 
ApaI G_GGCC'C

Cuts at:      0     169    3252 
   Size:        169    3083
 
ApoI r'AATT_y

Cuts at:      0       6     646    3252 
   Size:          6     640    2606

  Fragments arranged by size: 

                2606     640       6
 
AvaI C'yCGr_G

Cuts at:      0     162    1232    3252 
   Size:        162    1070    2020

  Fragments arranged by size: 

                2020    1070     162
 
AvrII C'CTAG_G

Cuts at:      0    1048    2469    3252 
   Size:       1048    1421     783

  Fragments arranged by size: 

                1421    1048     783
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2571    2604    3252 
   Size:       2571      33     648

  Fragments arranged by size: 

                2571     648      33
 
BanI G'GyrC_C

Cuts at:      0    3006    3252 
   Size:       3006     246
 
BanII G_rGCy'C

Cuts at:      0     169    1239    1302    1988    3252 
   Size:        169    1070      63     686    1264

  Fragments arranged by size: 

                1264    1070     686     169      63
 
BbvCI CC'TCA_GC

Cuts at:      0    2862    3074    3252 
   Size:       2862     212     178

  Fragments arranged by size: 

                2862     212     178
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2529    2563    3252 
   Size:       2529      34     689

  Fragments arranged by size: 

                2529     689      34
 
BclI T'GATC_A

Cuts at:      0    2282    3252 
   Size:       2282     970
 
BfrBI ATG'CAT

Cuts at:      0    1512    3252 
   Size:       1512    1740
 
BglI GCCn_nnn'nGGC

Cuts at:      0     692    3252 
   Size:        692    2560
 
BlpI GC'TnA_GC

Cuts at:      0    1238    3252 
   Size:       1238    2014
 
Bme1580I G_kGCm'C

Cuts at:      0     169     782    3252 
   Size:        169     613    2470

  Fragments arranged by size: 

                2470     613     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     140     342    3252 
   Size:        140     202    2910

  Fragments arranged by size: 

                2910     202     140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1338    3252 
   Size:       1338    1914
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422    1822    1912    2278    2639    2717    2744    2768 
   Size:        422    1400      90     366     361      78      27      24

Cuts at:   2768    2804    2900    2915    2951    3023    3041    3252 
   Size:         36      96      15      36      72      18     211

  Fragments arranged by size: 

                1400     422     366     361     211      96      90      78
                  72      36      36      27      24      18      15
 
Bpu10I CC'TnA_GC

Cuts at:      0    2862    2978    3068    3074    3252 
   Size:       2862     116      90       6     178

  Fragments arranged by size: 

                2862     178     116      90       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     540    1362    3252 
   Size:        540     822    1890

  Fragments arranged by size: 

                1890     822     540
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     485    1022    3252 
   Size:        485     537    2230

  Fragments arranged by size: 

                2230     537     485
 
BsaHI Gr'CG_yC

Cuts at:      0     435    3252 
   Size:        435    2817
 
BsaWI w'CCGG_w

Cuts at:      0    1334    1880    2955    3097    3252 
   Size:       1334     546    1075     142     155

  Fragments arranged by size: 

                1334    1075     546     155     142
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     573     603    1090    1120    3003    3027    3033    3039 
   Size:        573      30     487      30    1883      24       6       6

Cuts at:   3039    3057    3069    3252 
   Size:         18      12     183

  Fragments arranged by size: 

                1883     573     487     183      30      30      24      18
                  12       6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     281    2017    2074    2203    3252 
   Size:        281    1736      57     129    1049

  Fragments arranged by size: 

                1736    1049     281     129      57
 
BseYI C'CCAG_C

Cuts at:      0    1301    3252 
   Size:       1301    1951
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1958    3252 
   Size:       1958    1294
 
BsiHKAI G_wGCw'C

Cuts at:      0     591    1874    1988    3252 
   Size:        591    1283     114    1264

  Fragments arranged by size: 

                1283    1264     591     114
 
BsmI GAATG_Cn'

Cuts at:      0     302    2474    3252 
   Size:        302    2172     778

  Fragments arranged by size: 

                2172     778     302
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1108    1419    1882    2438    2786    3252 
   Size:       1108     311     463     556     348     466

  Fragments arranged by size: 

                1108     556     466     463     348     311
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     591     782    1239    1302    1874    1988    3252 
   Size:        169     422     191     457      63     572     114    1264

  Fragments arranged by size: 

                1264     572     457     422     191     169     114      63
            
 
BspEI T'CCGG_A

Cuts at:      0    1880    3252 
   Size:       1880    1372
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1052    3145    3252 
   Size:       1052    2093     107

  Fragments arranged by size: 

                2093    1052     107
 
BsrDI GCAATG_nn'

Cuts at:      0     889    3252 
   Size:        889    2363
 
BsrFI r'CCGG_y

Cuts at:      0    3097    3252 
   Size:       3097     155
 
BsrGI T'GTAC_A

Cuts at:      0    1830    3252 
   Size:       1830    1422
 
BssSI C'ACGA_G

Cuts at:      0    1435    3252 
   Size:       1435    1817
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      51    2632    3252 
   Size:         51    2581     620

  Fragments arranged by size: 

                2581     620      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2718    3183    3252 
   Size:       2718     465      69

  Fragments arranged by size: 

                2718     465      69
 
BstYI r'GATC_y

Cuts at:      0    1316    1527    3206    3252 
   Size:       1316     211    1679      46

  Fragments arranged by size: 

                1679    1316     211      46
 
BtgI C'CryG_G

Cuts at:      0      35    2717    3252 
   Size:         35    2682     535

  Fragments arranged by size: 

                2682     535      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     781    3252 
   Size:        781    2471
 
BtsI GCAGTG_nn'

Cuts at:      0     159    1676    2672    2687    2693    2789    3252 
   Size:        159    1517     996      15       6      96     463

  Fragments arranged by size: 

                1517     996     463     159      96      15       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3098    3133    3252 
   Size:       3098      35     119

  Fragments arranged by size: 

                3098     119      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2125    3252 
   Size:       2125    1127
 
EaeI y'GGCC_r

Cuts at:      0     548    1673    2207    2347    3252 
   Size:        548    1125     534     140     905

  Fragments arranged by size: 

                1125     905     548     534     140
 
EarI CTCTTCn'nnn_

Cuts at:      0    1766    3252 
   Size:       1766    1486
 
EcoICRI GAG'CTC

Cuts at:      0    1986    3252 
   Size:       1986    1266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     422     709    1822    1912    2278    2639    2717 
   Size:         32     390     287    1113      90     366     361      78

Cuts at:   2717    2744    2768    2804    2900    2915    2951    2999    3023 
   Size:         27      24      36      96      15      36      48      24

Cuts at:   3023    3041    3252 
   Size:         18     211

  Fragments arranged by size: 

                1113     390     366     361     287     211      96      90
                  78      48      36      36      32      27      24      24
                  18      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     166     709     953    1461    1731    1824    3252 
   Size:        166     543     244     508     270      93    1428

  Fragments arranged by size: 

                1428     543     508     270     244     166      93
 
EcoRI G'AATT_C

Cuts at:      0     646    3252 
   Size:        646    2606
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      82     685    3252 
   Size:         82     603    2567

  Fragments arranged by size: 

                2567     603      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     825     857    1524    1556    1697    1729    2001    2033 
   Size:        825      32     667      32     141      32     272      32

Cuts at:   2033    2320    2352    3126    3158    3252 
   Size:        287      32     774      32      94

  Fragments arranged by size: 

                 825     774     667     287     272     141      94      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1800    3252 
   Size:       1800    1452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     360    1334    3058    3070    3076    3252 
   Size:        360     974    1724      12       6     176

  Fragments arranged by size: 

                1724     974     360     176      12       6
 
MscI TGG'CCA

Cuts at:      0     550    1675    2209    3252 
   Size:        550    1125     534    1043

  Fragments arranged by size: 

                1125    1043     550     534
 
MslI CAynn'nnrTG

Cuts at:      0     374     476    2716    3252 
   Size:        374     102    2240     536

  Fragments arranged by size: 

                2240     536     374     102
 
MspA1I CmG'CkG

Cuts at:      0     285     450    2334    2629    2764    2797    2824    2845 
   Size:        285     165    1884     295     135      33      27      21

Cuts at:   2845    2866    2920    2974    3061    3252 
   Size:         21      54      54      87     191

  Fragments arranged by size: 

                1884     295     285     191     165     135      87      54
                  54      33      27      21      21
 
NcoI C'CATG_G

Cuts at:      0    2717    3252 
   Size:       2717     535
 
NdeI CA'TA_TG

Cuts at:      0    1425    3252 
   Size:       1425    1827
 
NsiI A_TGCA'T

Cuts at:      0    1514    3252 
   Size:       1514    1738
 
NspI r_CATG'y

Cuts at:      0     373     511     741    1850    2346    3252 
   Size:        373     138     230    1109     496     906

  Fragments arranged by size: 

                1109     906     496     373     230     138
 
PasI CC'CwG_GG

Cuts at:      0     240    3252 
   Size:        240    3012
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1308    3252 
   Size:       1308    1944
 
PfoI T'CCnGG_A

Cuts at:      0     459    2305    3102    3252 
   Size:        459    1846     797     150

  Fragments arranged by size: 

                1846     797     459     150
 
PpuMI rG'GwC_Cy

Cuts at:      0     953    3252 
   Size:        953    2299
 
PspOMI G'GGCC_C

Cuts at:      0     165    3252 
   Size:        165    3087
 
PstI C_TGCA'G

Cuts at:      0    1388    2587    2628    3252 
   Size:       1388    1199      41     624

  Fragments arranged by size: 

                1388    1199     624      41
 
PvuII CAG'CTG

Cuts at:      0     285    2334    2629    2764    2824    2845    2866    2920 
   Size:        285    2049     295     135      60      21      21      54

Cuts at:   2920    3061    3252 
   Size:        141     191

  Fragments arranged by size: 

                2049     295     285     191     141     135      60      54
                  21      21
 
SacI G_AGCT'C

Cuts at:      0    1988    3252 
   Size:       1988    1264
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1766    3252 
   Size:       1766    1486
 
ScaI AGT'ACT

Cuts at:      0     352    2291    3252 
   Size:        352    1939     961

  Fragments arranged by size: 

                1939     961     352
 
SfcI C'TryA_G

Cuts at:      0     752     786    1384    2583    2624    3252 
   Size:        752      34     598    1199      41     628

  Fragments arranged by size: 

                1199     752     628     598      41      34
 
SmaI CCC'GGG

Cuts at:      0     164    3252 
   Size:        164    3088
 
SmlI C'TyrA_G

Cuts at:      0     496     519    1341    3252 
   Size:        496      23     822    1911

  Fragments arranged by size: 

                1911     822     496      23
 
SphI G_CATG'C

Cuts at:      0     373     741    2346    3252 
   Size:        373     368    1605     906

  Fragments arranged by size: 

                1605     906     373     368
 
SspI AAT'ATT

Cuts at:      0    2053    3252 
   Size:       2053    1199
 
StyI C'CwwG_G

Cuts at:      0     335     660    1010    1048    1751    2469    2717    3252 
   Size:        335     325     350      38     703     718     248     535

  Fragments arranged by size: 

                 718     703     535     350     335     325     248      38
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2473    3252 
   Size:       2473     779
 
TatI w'GTAC_w

Cuts at:      0     350    1830    2289    3252 
   Size:        350    1480     459     963

  Fragments arranged by size: 

                1480     963     459     350
 
XbaI T'CTAG_A

Cuts at:      0    1542    2166    3252 
   Size:       1542     624    1086

  Fragments arranged by size: 

                1542    1086     624
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2599    3207    3252 
   Size:       2599     608      45

  Fragments arranged by size: 

                2599     608      45
 
XmaI C'CCGG_G

Cuts at:      0     162    3252 
   Size:        162    3090
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AflII     AgeI     AlfI    AlwNI     ApaI
     ApoI     AvaI    AvrII     BaeI     BanI    BanII    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PasI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmaI
     SmlI     SphI     SspI     StyI    TaqII     TatI     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI   AflIII     AhdI     AleI     AloI
    ApaLI     AscI     AseI    AsiSI    BamHI     BbeI     BbsI    BciVI
    BglII    BmgBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI
    BsrBI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI     DraI
   DraIII     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI     TstI  Tth111I
     XhoI     XmnI     ZraI
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