Restriction map for pF1KA1070
 (Linear) (Six-base) MAPSORT of: KIAA1070.seq  Check: 6641  from: 1  to: 2469



KIAA1070.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0    1318    1864    2469 
   Size:       1318     546     605

  Fragments arranged by size: 

                1318     605     546
 
Acc65I G'GTAC_C

Cuts at:      0    1827    2469 
   Size:       1827     642
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     848    1298    2469 
   Size:         97     751     450    1171

  Fragments arranged by size: 

                1171     751     450      97
 
AflII C'TTAA_G

Cuts at:      0     756    2469 
   Size:        756    1713
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2270    2469 
   Size:       2270     199
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1508    1540    2469 
   Size:       1508      32     929

  Fragments arranged by size: 

                1508     929      32
 
ApoI r'AATT_y

Cuts at:      0     215     567    1180    1248    1632    1681    1923    2469 
   Size:        215     352     613      68     384      49     242     546

  Fragments arranged by size: 

                 613     546     384     352     242     215      68      49
            
 
BanI G'GyrC_C

Cuts at:      0      76    1398    1827    2469 
   Size:         76    1322     429     642

  Fragments arranged by size: 

                1322     642     429      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     458    2469 
   Size:        458    2011
 
BbvCI CC'TCA_GC

Cuts at:      0    2141    2469 
   Size:       2141     328
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     591     625     652     686    2469 
   Size:        591      34      27      34    1783

  Fragments arranged by size: 

                1783     591      34      34      27
 
Bme1580I G_kGCm'C

Cuts at:      0      81    2469 
   Size:         81    2388
 
BmgBI CAC'GTC

Cuts at:      0    1940    2469 
   Size:       1940     529
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1335    1546    2469 
   Size:       1335     211     923

  Fragments arranged by size: 

                1335     923     211
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1150    2469 
   Size:       1150    1319
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    2423    2469 
   Size:        275    2148      46

  Fragments arranged by size: 

                2148     275      46
 
Bpu10I CC'TnA_GC

Cuts at:      0    2141    2469 
   Size:       2141     328
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672    1655    2469 
   Size:        672     983     814

  Fragments arranged by size: 

                 983     814     672
 
BsaAI yAC'GTr

Cuts at:      0      20    1448    2469 
   Size:         20    1428    1021

  Fragments arranged by size: 

                1428    1021      20
 
BsaBI GATnn'nnATC

Cuts at:      0     613    2219    2469 
   Size:        613    1606     250

  Fragments arranged by size: 

                1606     613     250
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1508    1538    1739    1769    2469 
   Size:       1508      30     201      30     700

  Fragments arranged by size: 

                1508     700     201      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2124    2313    2469 
   Size:       2124     189     156

  Fragments arranged by size: 

                2124     189     156
 
BseYI C'CCAG_C

Cuts at:      0     784     905    2469 
   Size:        784     121    1564

  Fragments arranged by size: 

                1564     784     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1628    2469 
   Size:       1628     841
 
BsmI GAATG_Cn'

Cuts at:      0    1000    2469 
   Size:       1000    1469
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1511    2469 
   Size:       1511     958
 
Bsp1286I G_dGCh'C

Cuts at:      0      81    2469 
   Size:         81    2388
 
BspHI T'CATG_A

Cuts at:      0    2223    2469 
   Size:       2223     246
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     812     933    2469 
   Size:        812     121    1536

  Fragments arranged by size: 

                1536     812     121
 
BsrDI GCAATG_nn'

Cuts at:      0     609     972    1470    2469 
   Size:        609     363     498     999

  Fragments arranged by size: 

                 999     609     498     363
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     934    2469 
   Size:        934    1535
 
BstEII G'GTnAC_C

Cuts at:      0     748     832    1648    2303    2469 
   Size:        748      84     816     655     166

  Fragments arranged by size: 

                 816     748     655     166      84
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     308    2469 
   Size:        308    2161
 
BstYI r'GATC_y

Cuts at:      0     775    1419    2469 
   Size:        775     644    1050

  Fragments arranged by size: 

                1050     775     644
 
BstZ17I GTA'TAC

Cuts at:      0    1319    1865    2469 
   Size:       1319     546     604

  Fragments arranged by size: 

                1319     604     546
 
BtgI C'CryG_G

Cuts at:      0     537    1512    2469 
   Size:        537     975     957

  Fragments arranged by size: 

                 975     957     537
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1220    2469 
   Size:       1220    1249
 
BtsI GCAGTG_nn'

Cuts at:      0       4      50    1952    2053    2469 
   Size:          4      46    1902     101     416

  Fragments arranged by size: 

                1902     416     101      46       4
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     318     353     939     974    2469 
   Size:        318      35     586      35    1495

  Fragments arranged by size: 

                1495     586     318      35      35
 
DraI TTT'AAA

Cuts at:      0     467    2469 
   Size:        467    2002
 
EaeI y'GGCC_r

Cuts at:      0      22     120     947    2469 
   Size:         22      98     827    1522

  Fragments arranged by size: 

                1522     827      98      22
 
EarI CTCTTCn'nnn_

Cuts at:      0    2174    2469 
   Size:       2174     295
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     275     848    1298    2423    2469 
   Size:         97     178     573     450    1125      46

  Fragments arranged by size: 

                1125     573     450     178      97      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     223     511    2469 
   Size:        223     288    1958

  Fragments arranged by size: 

                1958     288     223
 
EcoRV GAT'ATC

Cuts at:      0     318    1273    2469 
   Size:        318     955    1196

  Fragments arranged by size: 

                1196     955     318
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     406    1765    2469 
   Size:        406    1359     704

  Fragments arranged by size: 

                1359     704     406
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1201    1233    1411    1443    2256    2288    2469 
   Size:       1201      32     178      32     813      32     181

  Fragments arranged by size: 

                1201     813     181     178      32      32      32
 
HincII GTy'rAC

Cuts at:      0     624     801    1029    2469 
   Size:        624     177     228    1440

  Fragments arranged by size: 

                1440     624     228     177
 
HindIII A'AGCT_T

Cuts at:      0     707    2469 
   Size:        707    1762
 
KpnI G_GTAC'C

Cuts at:      0    1831    2469 
   Size:       1831     638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1799    2025    2469 
   Size:        820     979     226     444

  Fragments arranged by size: 

                 979     820     444     226
 
MscI TGG'CCA

Cuts at:      0      24     122     949    2469 
   Size:         24      98     827    1520

  Fragments arranged by size: 

                1520     827      98      24
 
MslI CAynn'nnrTG

Cuts at:      0    2228    2246    2344    2469 
   Size:       2228      18      98     125

  Fragments arranged by size: 

                2228     125      98      18
 
MspA1I CmG'CkG

Cuts at:      0     905    1405    1417    2469 
   Size:        905     500      12    1052

  Fragments arranged by size: 

                1052     905     500      12
 
NcoI C'CATG_G

Cuts at:      0     537    2469 
   Size:        537    1932
 
NdeI CA'TA_TG

Cuts at:      0     252     432    2469 
   Size:        252     180    2037

  Fragments arranged by size: 

                2037     252     180
 
NspI r_CATG'y

Cuts at:      0      56    2469 
   Size:         56    2413
 
PmlI CAC'GTG

Cuts at:      0      20    2469 
   Size:         20    2449
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1508    1540    2469 
   Size:       1508      32     929

  Fragments arranged by size: 

                1508     929      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     511    2469 
   Size:        511    1958
 
PvuII CAG'CTG

Cuts at:      0     905    1405    2469 
   Size:        905     500    1064

  Fragments arranged by size: 

                1064     905     500
 
RsrII CG'GwC_CG

Cuts at:      0    2264    2469 
   Size:       2264     205
 
SanDI GG'GwC_CC

Cuts at:      0     511    2469 
   Size:        511    1958
 
ScaI AGT'ACT

Cuts at:      0     641    2469 
   Size:        641    1828
 
SfcI C'TryA_G

Cuts at:      0    1000    1406    1559    2469 
   Size:       1000     406     153     910

  Fragments arranged by size: 

                1000     910     406     153
 
SmlI C'TyrA_G

Cuts at:      0     651     756    1670    2469 
   Size:        651     105     914     799

  Fragments arranged by size: 

                 914     799     651     105
 
SnaBI TAC'GTA

Cuts at:      0    1448    2469 
   Size:       1448    1021
 
SpeI A'CTAG_T

Cuts at:      0     721    2469 
   Size:        721    1748
 
SphI G_CATG'C

Cuts at:      0      56    2469 
   Size:         56    2413
 
StyI C'CwwG_G

Cuts at:      0     537    1164    2469 
   Size:        537     627    1305

  Fragments arranged by size: 

                1305     627     537
 
SwaI ATTT'AAAT

Cuts at:      0     467    2469 
   Size:        467    2002
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64    1383    2346    2469 
   Size:         64    1319     963     123

  Fragments arranged by size: 

                1319     963     123      64
 
TatI w'GTAC_w

Cuts at:      0     639     655    1533    2090    2469 
   Size:        639      16     878     557     379

  Fragments arranged by size: 

                 878     639     557     379      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1375    1407    2469 
   Size:       1375      32    1062

  Fragments arranged by size: 

                1375    1062      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      32     305     522    1063    2469 
   Size:         32     273     217     541    1406

  Fragments arranged by size: 

                1406     541     273     217      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1923    2469 
   Size:       1923     546
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII     AhdI     AloI     ApoI     BanI
     BbsI    BbvCI     BcgI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI
     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NspI     PmlI     PpiI    PpuMI    PvuII    RsrII    SanDI     ScaI
     SfcI     SmlI    SnaBI     SpeI     SphI     StyI     SwaI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BsaI    BsaHI    BsaWI    BsiEI  BsiHKAI
    BsiWI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   Bsu36I     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII     HpaI     KasI     MfeI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI     SacI    SacII     SalI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SrfI     SspI     StuI  Tth111I     XbaI     XhoI     XmaI     ZraI
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