Restriction map for pF1KA1047
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        AviII       
AvrII       BbeI        BfrI        BlnI        BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BsrFI       
BssHII      BstUI       Cfr10I      ClaI        CpoI        
DraIII      EcoRI       FseI        FspI        HincII      
HindII      HpaI        KasI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfoI        SgrAI       SnaBI       SphI        Sse8387I    
SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       3448  GGTACC               G^GTACC
AcyI         6786  GACGCC               G[GA]^CG[CT]C
AflIII       4391  ACACGT               A^C[GA][CT]GT
AhdI         3741  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3403  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2698  AGCGCT               AGC^GCT
Asp718I      3448  GGTACC               G^GTACC
AspEI        3741  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         4940  TGGCCA               TGG^CCA
BbrPI        2691  CACGTG               CAC^GTG
BbvCI         408  GCTGAGG              CCTCAGC(-5/-2)
BsaAI        2691  CACGTG               [CT]AC^GT[GA]
BsaHI        6786  GACGCC               G[GA]^CG[CT]C
BsaWI        6156  TCCGGT               [AT]^CCGG[AT]
BsiEI        4171  CGGCCG               CG[GA][CT]^CG
Bsp1407I     6756  TGTACA               T^GTACA
BsrGI        6756  TGTACA               T^GTACA
BssSI        2069  CTCGTG               CACGAG(-5/-1)
Bst1107I       78  GTATAC               GTA^TAC
BstEII       4834  GGTTACC              G^GT[ACGT]ACC
BstPI        4834  GGTTACC              G^GT[ACGT]ACC
BstXI        4742  CCACGCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I        78  GTATAC               GTA^TAC
Bsu36I       3403  CCTGAGG              CC^T[ACGT]AGG
EagI         4171  CGGCCG               C^GGCCG
Eam1105I     3741  GACTAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        4171  CGGCCG               C^GGCCG
Eco47III     2698  AGCGCT               AGC^GCT
Eco52I       4171  CGGCCG               C^GGCCG
Eco81I       3403  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4834  GGTTACC              G^GT[ACGT]ACC
EcoRV        5571  GATATC               GAT^ATC
HaeII        2698  AGCGCT               [GA]GCGC^[CT]
Hin1I        6786  GACGCC               G[GA]^CG[CT]C
KpnI         3448  GGTACC               GGTAC^C
MluNI        4940  TGGCCA               TGG^CCA
MscI         4940  TGGCCA               TGG^CCA
NspI         6895  GCATGT               [GA]CATG^[CT]
PaeR7I       2276  CTCGAG               C^TCGAG
PmaCI        2691  CACGTG               CAC^GTG
PmlI         2691  CACGTG               CAC^GTG
PshAI        3628  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         6874  AGTACT               AGT^ACT
SmaI         1706  CCCGGG               CCC^GGG
SpeI          259  ACTAGT               A^CTAGT
SspBI        6756  TGTACA               T^GTACA
SspI         3718  AATATT               AAT^ATT
XbaI         6436  TCTAGA               T^CTAGA
XhoI         2276  CTCGAG               C^TCGAG
XmaCI        1706  CCCGGG               C^CCGGG
XmaI         1706  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI           78  GTATAC               GT^[AC][GT]AC
             4581  GTCTAC              
Alw44I        388  GTGCAC               G^TGCAC
             4487  GTGCAC              
ApaI         3422  GGGCCC               GGGCC^C
             5451  GGGCCC              
ApaLI         388  GTGCAC               G^TGCAC
             4487  GTGCAC              
BcgI          186  GCAACAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1509  GCAAATTGCTCG        
BciVI        2869  GTATCC               GTATCC(6/5)
             4631  GTATCC              
BglII        1337  AGATCT               A^GATCT
             6392  AGATCT              
BsmBI        2793  CGTCTC               CGTCTC(1/5)
             6114  CGTCTC              
BstBI         194  TTCGAA               TT^CGAA
             1046  TTCGAA              
DraI          877  TTTAAA               TTT^AAA
             3774  TTTAAA              
DrdI         3915  GACTGTATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5288  GACCTGGCAGTC        
EcoT22I      2543  ATGCAT               ATGCA^T
             2947  ATGCAT              
HgaI         6786  GACGC                GACGC(5/10)
             4929  GCGTC               
MfeI         1946  CAATTG               C^AATTG
             2894  CAATTG              
MunI         1946  CAATTG               C^AATTG
             2894  CAATTG              
NcoI         5069  CCATGG               C^CATGG
             6299  CCATGG              
NdeI         3710  CATATG               CA^TATG
             4058  CATATG              
NsiI         2543  ATGCAT               ATGCA^T
             2947  ATGCAT              
NspV          194  TTCGAA               TT^CGAA
             1046  TTCGAA              
SapI         1813  GAAGAGC              GCTCTTC(1/4)
             2074  GAAGAGC             
SfuI          194  TTCGAA               TT^CGAA
             1046  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AspI        
AvaI        AvaII       BamHI       BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com