Restriction map for pF1KA1047
 (Linear) (Six-base) MAPSORT of: KIAA1047.seq  Check: 3620  from: 1  to: 7011



KIAA1047.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4745    5643    7011 
   Size:       4745     898    1368

  Fragments arranged by size: 

                4745    1368     898
 
AccI GT'mk_AC

Cuts at:      0      79    4582    7011 
   Size:         79    4503    2429

  Fragments arranged by size: 

                4503    2429      79
 
Acc65I G'GTAC_C

Cuts at:      0    3448    7011 
   Size:       3448    3563
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1832    2393    2483    2821    3052    3125    3167    5309 
   Size:       1832     561      90     338     231      73      42    2142

Cuts at:   5309    6728    7011 
   Size:       1419     283

  Fragments arranged by size: 

                2142    1832    1419     561     338     283     231      90
                  73      42
 
AfeI AGC'GCT

Cuts at:      0    2700    7011 
   Size:       2700    4311
 
AflIII A'CryG_T

Cuts at:      0    4391    7011 
   Size:       4391    2620
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3746    7011 
   Size:       3746    3265
 
AleI CACnn'nnGTG

Cuts at:      0    4396    7011 
   Size:       4396    2615
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2663    2864    7011 
   Size:       2663     201    4147

  Fragments arranged by size: 

                4147    2663     201
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     578     610    5046    5078    7011 
   Size:        578      32    4436      32    1933

  Fragments arranged by size: 

                4436    1933     578      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     121     235    1864    2293    4654    4795    4971    5173 
   Size:        121     114    1629     429    2361     141     176     202

Cuts at:   5173    5536    6214    6388    6947    7011 
   Size:        363     678     174     559      64

  Fragments arranged by size: 

                2361    1629     678     559     429     363     202     176
                 174     141     121     114      64
 
ApaI G_GGCC'C

Cuts at:      0    3426    5455    7011 
   Size:       3426    2029    1556

  Fragments arranged by size: 

                3426    2029    1556
 
ApaLI G'TGCA_C

Cuts at:      0     388    4487    7011 
   Size:        388    4099    2524

  Fragments arranged by size: 

                4099    2524     388
 
ApoI r'AATT_y

Cuts at:      0     220     755    1043    3373    3503    7011 
   Size:        220     535     288    2330     130    3508

  Fragments arranged by size: 

                3508    2330     535     288     220     130
 
AvaI C'yCGr_G

Cuts at:      0    1706    2276    2659    3482    3731    7011 
   Size:       1706     570     383     823     249    3280

  Fragments arranged by size: 

                3280    1706     823     570     383     249
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     803     836    7011 
   Size:        803      33    6175

  Fragments arranged by size: 

                6175     803      33
 
BamHI G'GATC_C

Cuts at:      0     435    4752    4782    5409    7011 
   Size:        435    4317      30     627    1602

  Fragments arranged by size: 

                4317    1602     627     435      30
 
BanI G'GyrC_C

Cuts at:      0    2529    3448    3550    4036    7011 
   Size:       2529     919     102     486    2975

  Fragments arranged by size: 

                2975    2529     919     486     102
 
BanII G_rGCy'C

Cuts at:      0    1160    1820    2312    2666    3426    3538    4465    5455 
   Size:       1160     660     492     354     760     112     927     990

Cuts at:   5455    6128    6137    7011 
   Size:        673       9     874

  Fragments arranged by size: 

                1160     990     927     874     760     673     660     492
                 354     112       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1631    2177    3632    6488    6812    7011 
   Size:       1631     546    1455    2856     324     199

  Fragments arranged by size: 

                2856    1631    1455     546     324     199
 
BbvCI CC'TCA_GC

Cuts at:      0     409    7011 
   Size:        409    6602
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     175     209    1498    1532    7011 
   Size:        175      34    1289      34    5479

  Fragments arranged by size: 

                5479    1289     175      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2880    4642    7011 
   Size:       2880    1762    2369

  Fragments arranged by size: 

                2880    2369    1762
 
BclI T'GATC_A

Cuts at:      0     501     933    5834    5847    6668    7011 
   Size:        501     432    4901      13     821     343

  Fragments arranged by size: 

                4901     821     501     432     343      13
 
BfrBI ATG'CAT

Cuts at:      0    2545    2949    7011 
   Size:       2545     404    4062

  Fragments arranged by size: 

                4062    2545     404
 
BglI GCCn_nnn'nGGC

Cuts at:      0     405    1745    1804    2730    5291    7011 
   Size:        405    1340      59     926    2561    1720

  Fragments arranged by size: 

                2561    1720    1340     926     405      59
 
BglII A'GATC_T

Cuts at:      0    1337    6392    7011 
   Size:       1337    5055     619

  Fragments arranged by size: 

                5055    1337     619
 
BlpI GC'TnA_GC

Cuts at:      0    2636    5420    6377    6868    7011 
   Size:       2636    2784     957     491     143

  Fragments arranged by size: 

                2784    2636     957     491     143
 
Bme1580I G_kGCm'C

Cuts at:      0     392    3426    3553    4204    4491    5455    5819    7011 
   Size:        392    3034     127     651     287     964     364    1192

  Fragments arranged by size: 

                3034    1192     964     651     392     364     287     127
            
 
BmgBI CAC'GTC

Cuts at:      0     328    4394    6114    7011 
   Size:        328    4066    1720     897

  Fragments arranged by size: 

                4066    1720     897     328
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     286    1947    2324    2661    2899    4981    5357    7011 
   Size:        286    1661     377     337     238    2082     376    1654

  Fragments arranged by size: 

                2082    1661    1654     377     376     337     286     238
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3851    6156    7011 
   Size:       3851    2305     855

  Fragments arranged by size: 

                3851    2305     855
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2986    4133    4631    4814    5129    6035    7011 
   Size:       2986    1147     498     183     315     906     976

  Fragments arranged by size: 

                2986    1147     976     906     498     315     183
 
Bpu10I CC'TnA_GC

Cuts at:      0     409     650    2123    2346    7011 
   Size:        409     241    1473     223    4665

  Fragments arranged by size: 

                4665    1473     409     241     223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230    2326    3257    7011 
   Size:        230    2096     931    3754

  Fragments arranged by size: 

                3754    2096     931     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1864    2383    4366    4981    5335    5352    6051    6982 
   Size:       1864     519    1983     615     354      17     699     931

Cuts at:   6982    7011 
   Size:         29

  Fragments arranged by size: 

                1983    1864     931     699     615     519     354      29
                  17
 
BsaAI yAC'GTr

Cuts at:      0    2693    7011 
   Size:       2693    4318
 
BsaBI GATnn'nnATC

Cuts at:      0     725    2441    4694    7011 
   Size:        725    1716    2253    2317

  Fragments arranged by size: 

                2317    2253    1716     725
 
BsaHI Gr'CG_yC

Cuts at:      0    6787    7011 
   Size:       6787     224
 
BsaWI w'CCGG_w

Cuts at:      0    6156    7011 
   Size:       6156     855
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     246     276    4961    4991    5046    5076    5097    5127 
   Size:        246      30    4685      30      55      30      21      30

Cuts at:   5127    5332    5362    7011 
   Size:        205      30    1649

  Fragments arranged by size: 

                4685    1649     246     205      55      30      30      30
                  30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      53     665    2983    5054    6120    6333    6662    6741 
   Size:         53     612    2318    2071    1066     213     329      79

Cuts at:   6741    6917    7011 
   Size:        176      94

  Fragments arranged by size: 

                2318    2071    1066     612     329     213     176      94
                  79      53
 
BseYI C'CCAG_C

Cuts at:      0    2245    4635    4986    5446    5909    6551    7011 
   Size:       2245    2390     351     460     463     642     460

  Fragments arranged by size: 

                2390    2245     642     463     460     460     351
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2204    2309    2715    2859    5522    7011 
   Size:       2204     105     406     144    2663    1489

  Fragments arranged by size: 

                2663    2204    1489     406     144     105
 
BsiEI CG_ry'CG

Cuts at:      0    4174    7011 
   Size:       4174    2837
 
BsiHKAI G_wGCw'C

Cuts at:      0     392    2214    2456    4168    4491    6903    7011 
   Size:        392    1822     242    1712     323    2412     108

  Fragments arranged by size: 

                2412    1822    1712     392     323     242     108
 
BsmI GAATG_Cn'

Cuts at:      0      34     442    3518    3620    3732    4301    7011 
   Size:         34     408    3076     102     112     569    2710

  Fragments arranged by size: 

                3076    2710     569     408     112     102      34
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2799    6120    7011 
   Size:       2799    3321     891

  Fragments arranged by size: 

                3321    2799     891
 
Bsp1286I G_dGCh'C

Cuts at:      0     392    1160    1820    2214    2312    2456    2666    3426 
   Size:        392     768     660     394      98     144     210     760

Cuts at:   3426    3538    3553    4168    4204    4465    4491    5455    5819 
   Size:        112      15     615      36     261      26     964     364

Cuts at:   5819    6128    6137    6903    7011 
   Size:        309       9     766     108

  Fragments arranged by size: 

                 964     768     766     760     660     615     394     392
                 364     309     261     210     144     112     108      98
                  36      26      15       9
 
BspHI T'CATG_A

Cuts at:      0     300    3762    4101    4590    7011 
   Size:        300    3462     339     489    2421

  Fragments arranged by size: 

                3462    2421     489     339     300
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2301    4745    5267    5643    7011 
   Size:       2301    2444     522     376    1368

  Fragments arranged by size: 

                2444    2301    1368     522     376
 
BsrBI CCG'CTC

Cuts at:      0    2912    5205    5217    5223    6239    7011 
   Size:       2912    2293      12       6    1016     772

  Fragments arranged by size: 

                2912    2293    1016     772      12       6
 
BsrDI GCAATG_nn'

Cuts at:      0    1115    2951    4868    7011 
   Size:       1115    1836    1917    2143

  Fragments arranged by size: 

                2143    1917    1836    1115
 
BsrGI T'GTAC_A

Cuts at:      0    6756    7011 
   Size:       6756     255
 
BssSI C'ACGA_G

Cuts at:      0    2069    7011 
   Size:       2069    4942
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     396    1948    4795    5173    5536    6214    7011 
   Size:        396    1552    2847     378     363     678     797

  Fragments arranged by size: 

                2847    1552     797     678     396     378     363
 
BstBI TT'CG_AA

Cuts at:      0     195    1047    7011 
   Size:        195     852    5964

  Fragments arranged by size: 

                5964     852     195
 
BstEII G'GTnAC_C

Cuts at:      0    4834    7011 
   Size:       4834    2177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4749    7011 
   Size:       4749    2262
 
BstYI r'GATC_y

Cuts at:      0     435     593    1337    2782    2823    3298    4752    4782 
   Size:        435     158     744    1445      41     475    1454      30

Cuts at:   4782    5409    6392    6944    7011 
   Size:        627     983     552      67

  Fragments arranged by size: 

                1454    1445     983     744     627     552     475     435
                 158      67      41      30
 
BstZ17I GTA'TAC

Cuts at:      0      80    7011 
   Size:         80    6931
 
Bsu36I CC'TnA_GG

Cuts at:      0    3404    7011 
   Size:       3404    3607
 
BtgI C'CryG_G

Cuts at:      0    2315    3319    4290    5069    6299    7011 
   Size:       2315    1004     971     779    1230     712

  Fragments arranged by size: 

                2315    1230    1004     971     779     712
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    5615    7011 
   Size:       5615    1396
 
BtsI GCAGTG_nn'

Cuts at:      0    4432    4709    5437    5492    6967    7011 
   Size:       4432     277     728      55    1475      44

  Fragments arranged by size: 

                4432    1475     728     277      55      44
 
DraI TTT'AAA

Cuts at:      0     879    3776    7011 
   Size:        879    2897    3235

  Fragments arranged by size: 

                3235    2897     879
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3921    5294    7011 
   Size:       3921    1373    1717

  Fragments arranged by size: 

                3921    1717    1373
 
EaeI y'GGCC_r

Cuts at:      0    4171    4940    5263    5283    7011 
   Size:       4171     769     323      20    1728

  Fragments arranged by size: 

                4171    1728     769     323      20
 
EagI C'GGCC_G

Cuts at:      0    4171    7011 
   Size:       4171    2840
 
EarI CTCTTCn'nnn_

Cuts at:      0     530     626    1399    1547    1598    1808    2069    2459 
   Size:        530      96     773     148      51     210     261     390

Cuts at:   2459    6123    6609    6747    6822    7011 
   Size:       3664     486     138      75     189

  Fragments arranged by size: 

                3664     773     530     486     390     261     210     189
                 148     138      96      75      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1832    2393    2483    2821    2986    3052    3125    3167 
   Size:       1832     561      90     338     165      66      73      42

Cuts at:   3167    4133    4631    4814    5129    5309    6035    6728    7011 
   Size:        966     498     183     315     180     726     693     283

  Fragments arranged by size: 

                1832     966     726     693     561     498     338     315
                 283     183     180     165      90      73      66      42
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3641    5045    6641    6713    7011 
   Size:       3641    1404    1596      72     298

  Fragments arranged by size: 

                3641    1596    1404     298      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1275    3422    4273    4496    5451    5452    5822    6627 
   Size:       1275    2147     851     223     955       1     370     805

Cuts at:   6627    6812    7011 
   Size:        185     199

  Fragments arranged by size: 

                2147    1275     955     851     805     370     223     199
                 185       1
 
EcoRV GAT'ATC

Cuts at:      0    5573    7011 
   Size:       5573    1438
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     221    1356    2123    2231    3997    7011 
   Size:        221    1135     767     108    1766    3014

  Fragments arranged by size: 

                3014    1766    1135     767     221     108
 
HaeII r_GCGC'y

Cuts at:      0    2702    7011 
   Size:       2702    4309
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     540     572    1144    1176    2653    2685    2774    2806 
   Size:        540      32     572      32    1477      32      89      32

Cuts at:   2806    3081    3113    3200    3232    3732    3764    4210    4242 
   Size:        275      32      87      32     500      32     446      32

Cuts at:   4242    4665    4697    4868    4900    5562    5594    6223    6255 
   Size:        423      32     171      32     662      32     629      32

Cuts at:   6255    6384    6416    6799    6831    6979    7011 
   Size:        129      32     383      32     148      32

  Fragments arranged by size: 

                1477     662     629     572     540     500     446     423
                 383     275     171     148     129      89      87      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    3966    6280    6510    7011 
   Size:       3966    2314     230     501

  Fragments arranged by size: 

                3966    2314     501     230
 
KpnI G_GTAC'C

Cuts at:      0    3452    7011 
   Size:       3452    3559
 
MfeI C'AATT_G

Cuts at:      0    1946    2894    7011 
   Size:       1946     948    4117

  Fragments arranged by size: 

                4117    1946     948
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3145    3517    4120    5050    5273    5437    6977    7011 
   Size:       3145     372     603     930     223     164    1540      34

  Fragments arranged by size: 

                3145    1540     930     603     372     223     164      34
            
 
MscI TGG'CCA

Cuts at:      0    4942    7011 
   Size:       4942    2069
 
MslI CAynn'nnrTG

Cuts at:      0    1255    3434    4330    4396    5303    5585    7011 
   Size:       1255    2179     896      66     907     282    1426

  Fragments arranged by size: 

                2179    1426    1255     907     896     282      66
 
MspA1I CmG'CkG

Cuts at:      0     396    1783    1804    2371    2976    4305    4792    5170 
   Size:        396    1387      21     567     605    1329     487     378

Cuts at:   5170    5778    6373    7011 
   Size:        608     595     638

  Fragments arranged by size: 

                1387    1329     638     608     605     595     567     487
                 396     378      21
 
NcoI C'CATG_G

Cuts at:      0    5069    6299    7011 
   Size:       5069    1230     712

  Fragments arranged by size: 

                5069    1230     712
 
NdeI CA'TA_TG

Cuts at:      0    3711    4059    7011 
   Size:       3711     348    2952

  Fragments arranged by size: 

                3711    2952     348
 
NsiI A_TGCA'T

Cuts at:      0    2547    2951    7011 
   Size:       2547     404    4060

  Fragments arranged by size: 

                4060    2547     404
 
NspI r_CATG'y

Cuts at:      0    6899    7011 
   Size:       6899     112
 
PasI CC'CwG_GG

Cuts at:      0    3881    4197    4465    7011 
   Size:       3881     316     268    2546

  Fragments arranged by size: 

                3881    2546     316     268
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4063    4654    6558    7011 
   Size:       4063     591    1904     453

  Fragments arranged by size: 

                4063    1904     591     453
 
PfoI T'CCnGG_A

Cuts at:      0    2963    5145    7011 
   Size:       2963    2182    1866

  Fragments arranged by size: 

                2963    2182    1866
 
PmlI CAC'GTG

Cuts at:      0    2693    7011 
   Size:       2693    4318
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5046    5078    7011 
   Size:       5046      32    1933

  Fragments arranged by size: 

                5046    1933      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1275    4496    5822    6627    7011 
   Size:       1275    3221    1326     805     384

  Fragments arranged by size: 

                3221    1326    1275     805     384
 
PshAI GACnn'nnGTC

Cuts at:      0    3632    7011 
   Size:       3632    3379
 
PsiI TTA'TAA

Cuts at:      0    1460    3202    6845    7011 
   Size:       1460    1742    3643     166

  Fragments arranged by size: 

                3643    1742    1460     166
 
PspOMI G'GGCC_C

Cuts at:      0    3422    5451    7011 
   Size:       3422    2029    1560

  Fragments arranged by size: 

                3422    2029    1560
 
PstI C_TGCA'G

Cuts at:      0     423    1782    1797    4716    4791    5241    5532    5660 
   Size:        423    1359      15    2919      75     450     291     128

Cuts at:   5660    6219    7011 
   Size:        559     792

  Fragments arranged by size: 

                2919    1359     792     559     450     423     291     128
                  75      15
 
PvuII CAG'CTG

Cuts at:      0    1783    2371    5170    5778    6373    7011 
   Size:       1783     588    2799     608     595     638

  Fragments arranged by size: 

                2799    1783     638     608     595     588
 
SanDI GG'GwC_CC

Cuts at:      0    6627    7011 
   Size:       6627     384
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1808    2069    7011 
   Size:       1808     261    4942

  Fragments arranged by size: 

                4942    1808     261
 
ScaI AGT'ACT

Cuts at:      0    6876    7011 
   Size:       6876     135
 
SexAI A'CCwGG_T

Cuts at:      0     845    1756    6033    7011 
   Size:        845     911    4277     978

  Fragments arranged by size: 

                4277     978     911     845
 
SfcI C'TryA_G

Cuts at:      0     419    1778    1793    1856    4712    4787    5237    5528 
   Size:        419    1359      15      63    2856      75     450     291

Cuts at:   5528    5656    6215    7011 
   Size:        128     559     796

  Fragments arranged by size: 

                2856    1359     796     559     450     419     291     128
                  75      63      15
 
SmaI CCC'GGG

Cuts at:      0    1708    7011 
   Size:       1708    5303
 
SmlI C'TyrA_G

Cuts at:      0     245    2276    2305    3272    7011 
   Size:        245    2031      29     967    3739

  Fragments arranged by size: 

                3739    2031     967     245      29
 
SpeI A'CTAG_T

Cuts at:      0     259    7011 
   Size:        259    6752
 
SspI AAT'ATT

Cuts at:      0    3720    7011 
   Size:       3720    3291
 
StuI AGG'CCT

Cuts at:      0     243    5471    6087    7011 
   Size:        243    5228     616     924

  Fragments arranged by size: 

                5228     924     616     243
 
StyI C'CwwG_G

Cuts at:      0      27     815    1643    4111    5069    5373    5466    6299 
   Size:         27     788     828    2468     958     304      93     833

Cuts at:   6299    7011 
   Size:        712

  Fragments arranged by size: 

                2468     958     833     828     788     712     304      93
                  27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     242    2343    3297    4551    5168    6312    7011 
   Size:        242    2101     954    1254     617    1144     699

  Fragments arranged by size: 

                2101    1254    1144     954     699     617     242
 
TatI w'GTAC_w

Cuts at:      0    2332    2359    6756    6874    7011 
   Size:       2332      27    4397     118     137

  Fragments arranged by size: 

                4397    2332     137     118      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2891    2923    4961    4993    5073    5105    7011 
   Size:       2891      32    2038      32      80      32    1906

  Fragments arranged by size: 

                2891    2038    1906      80      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     326    4666    6993    7011 
   Size:        326    4340    2327      18

  Fragments arranged by size: 

                4340    2327     326      18
 
XbaI T'CTAG_A

Cuts at:      0    6436    7011 
   Size:       6436     575
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2601    3053    3174    3291    4083    5380    6797    7011 
   Size:       2601     452     121     117     792    1297    1417     214

  Fragments arranged by size: 

                2601    1417    1297     792     452     214     121     117
            
 
XhoI C'TCGA_G

Cuts at:      0    2276    7011 
   Size:       2276    4735
 
XmaI C'CCGG_G

Cuts at:      0    1706    7011 
   Size:       1706    5305
 
XmnI GAAnn'nnTTC

Cuts at:      0     272    1073    3065    3110    7011 
   Size:        272     801    1992      45    3901

  Fragments arranged by size: 

                3901    1992     801     272      45
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI    PvuII    SanDI     SapI     ScaI    SexAI     SfcI
     SmaI     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AscI     AseI    AsiSI    AvrII
     BbeI     BmtI    BsiWI    BspEI    BsrFI   BssHII     ClaI    CspCI
   DraIII     EciI  EcoICRI    EcoRI     FseI     FspI    FspAI   HincII
     HpaI     KasI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PmeI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SphI     SrfI     SwaI     ZraI
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