Restriction map for
pF1KA1012
(Linear) (Six-base) MAPSORT of: KIAA1012.seq Check: 8630 from: 1 to: 4305
KIAA1012.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 3005 4305
Size: 3005 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 883 2438 4305
Size: 376 507 1555 1867
Fragments arranged by size:
1867 1555 507 376
AflII C'TTAA_G
Cuts at: 0 127 4305
Size: 127 4178
AleI CACnn'nnGTG
Cuts at: 0 527 4305
Size: 527 3778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1656 4305
Size: 1656 2649
AlwNI CAG_nnn'CTG
Cuts at: 0 1405 1564 2923 4305
Size: 1405 159 1359 1382
Fragments arranged by size:
1405 1382 1359 159
ApaLI G'TGCA_C
Cuts at: 0 1613 4305
Size: 1613 2692
ApoI r'AATT_y
Cuts at: 0 198 418 515 813 1040 2636 2806 3355
Size: 198 220 97 298 227 1596 170 549
Cuts at: 3355 3862 4269 4305
Size: 507 407 36
Fragments arranged by size:
1596 549 507 407 298 227 220 198
170 97 36
AseI AT'TA_AT
Cuts at: 0 683 1208 1808 1886 2441 4305
Size: 683 525 600 78 555 1864
Fragments arranged by size:
1864 683 600 555 525 78
AvrII C'CTAG_G
Cuts at: 0 1429 4213 4305
Size: 1429 2784 92
Fragments arranged by size:
2784 1429 92
BanI G'GyrC_C
Cuts at: 0 1381 1823 4305
Size: 1381 442 2482
Fragments arranged by size:
2482 1381 442
BanII G_rGCy'C
Cuts at: 0 891 2545 4305
Size: 891 1654 1760
Fragments arranged by size:
1760 1654 891
BbeI G_GCGC'C
Cuts at: 0 1827 4305
Size: 1827 2478
BbsI GAAGACnn'nnnn_
Cuts at: 0 3755 3931 4305
Size: 3755 176 374
Fragments arranged by size:
3755 374 176
BbvCI CC'TCA_GC
Cuts at: 0 109 239 2916 4305
Size: 109 130 2677 1389
Fragments arranged by size:
2677 1389 130 109
BciVI GTATCCnnnnn_n'
Cuts at: 0 1250 1449 4305
Size: 1250 199 2856
Fragments arranged by size:
2856 1250 199
BclI T'GATC_A
Cuts at: 0 1029 1356 2094 4286 4305
Size: 1029 327 738 2192 19
Fragments arranged by size:
2192 1029 738 327 19
BfrBI ATG'CAT
Cuts at: 0 1681 4305
Size: 1681 2624
BglII A'GATC_T
Cuts at: 0 955 4305
Size: 955 3350
Bme1580I G_kGCm'C
Cuts at: 0 1617 4305
Size: 1617 2688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1166 4305
Size: 1166 3139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 730 4305
Size: 266 464 3575
Fragments arranged by size:
3575 464 266
Bpu10I CC'TnA_GC
Cuts at: 0 109 239 2916 4305
Size: 109 130 2677 1389
Fragments arranged by size:
2677 1389 130 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2158 2622 3879 4305
Size: 2158 464 1257 426
Fragments arranged by size:
2158 1257 464 426
BsaI GGTCTCn'nnnn_
Cuts at: 0 1793 4305
Size: 1793 2512
BsaAI yAC'GTr
Cuts at: 0 189 3109 4305
Size: 189 2920 1196
Fragments arranged by size:
2920 1196 189
BsaBI GATnn'nnATC
Cuts at: 0 1601 4291 4305
Size: 1601 2690 14
Fragments arranged by size:
2690 1601 14
BsaHI Gr'CG_yC
Cuts at: 0 1824 4305
Size: 1824 2481
BsaWI w'CCGG_w
Cuts at: 0 254 4305
Size: 254 4051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2405 2435 3071 3101 4305
Size: 2405 30 636 30 1204
Fragments arranged by size:
2405 1204 636 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3305 4305
Size: 3305 1000
BseYI C'CCAG_C
Cuts at: 0 230 4305
Size: 230 4075
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 90 620 1318 3109 3605 4305
Size: 43 47 530 698 1791 496 700
Fragments arranged by size:
1791 700 698 530 496 47 43
BsiHKAI G_wGCw'C
Cuts at: 0 891 1428 1617 2571 4305
Size: 891 537 189 954 1734
Fragments arranged by size:
1734 954 891 537 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 103 4305
Size: 103 4202
Bsp1286I G_dGCh'C
Cuts at: 0 891 1428 1617 2545 2571 4305
Size: 891 537 189 928 26 1734
Fragments arranged by size:
1734 928 891 537 189 26
BspEI T'CCGG_A
Cuts at: 0 254 4305
Size: 254 4051
BspHI T'CATG_A
Cuts at: 0 414 4305
Size: 414 3891
BsrBI CCG'CTC
Cuts at: 0 87 4305
Size: 87 4218
BsrDI GCAATG_nn'
Cuts at: 0 1370 1638 2323 4305
Size: 1370 268 685 1982
Fragments arranged by size:
1982 1370 685 268
BsrGI T'GTAC_A
Cuts at: 0 12 2974 4305
Size: 12 2962 1331
Fragments arranged by size:
2962 1331 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1381 1405 4305
Size: 1381 24 2900
Fragments arranged by size:
2900 1381 24
BstBI TT'CG_AA
Cuts at: 0 1608 4305
Size: 1608 2697
BstXI CCAn_nnnn'nTGG
Cuts at: 0 731 4305
Size: 731 3574
BstYI r'GATC_y
Cuts at: 0 168 726 955 2709 4305
Size: 168 558 229 1754 1596
Fragments arranged by size:
1754 1596 558 229 168
BtgI C'CryG_G
Cuts at: 0 361 1664 4092 4305
Size: 361 1303 2428 213
Fragments arranged by size:
2428 1303 361 213
BtsI GCAGTG_nn'
Cuts at: 0 1406 2131 3206 3705 4305
Size: 1406 725 1075 499 600
Fragments arranged by size:
1406 1075 725 600 499
ClaI AT'CG_AT
Cuts at: 0 1707 4305
Size: 1707 2598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 884 919 2249 2284 4305
Size: 884 35 1330 35 2021
Fragments arranged by size:
2021 1330 884 35 35
DraI TTT'AAA
Cuts at: 0 1538 3236 4305
Size: 1538 1698 1069
Fragments arranged by size:
1698 1538 1069
DraIII CAC_nnn'GTG
Cuts at: 0 3366 4305
Size: 3366 939
DrdI GACnn_nn'nnGTC
Cuts at: 0 46 2319 4305
Size: 46 2273 1986
Fragments arranged by size:
2273 1986 46
EaeI y'GGCC_r
Cuts at: 0 2037 3503 4305
Size: 2037 1466 802
Fragments arranged by size:
2037 1466 802
EarI CTCTTCn'nnn_
Cuts at: 0 2576 2663 4305
Size: 2576 87 1642
Fragments arranged by size:
2576 1642 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1232 4305
Size: 1232 3073
EcoICRI GAG'CTC
Cuts at: 0 889 4305
Size: 889 3416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 376 730 883 2438 4305
Size: 266 110 354 153 1555 1867
Fragments arranged by size:
1867 1555 354 266 153 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 296 4305
Size: 296 4009
EcoO109I rG'GnC_Cy
Cuts at: 0 1074 2644 4305
Size: 1074 1570 1661
Fragments arranged by size:
1661 1570 1074
EcoRI G'AATT_C
Cuts at: 0 515 813 1040 3355 4269 4305
Size: 515 298 227 2315 914 36
Fragments arranged by size:
2315 914 515 298 227 36
EcoRV GAT'ATC
Cuts at: 0 1482 3540 4305
Size: 1482 2058 765
Fragments arranged by size:
2058 1482 765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 977 1366 1384 2320 3486 3626 4305
Size: 977 389 18 936 1166 140 679
Fragments arranged by size:
1166 977 936 679 389 140 18
HaeII r_GCGC'y
Cuts at: 0 1827 4305
Size: 1827 2478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 711 726 743 758 2098 2130 2459 2491
Size: 711 15 17 15 1340 32 329 32
Cuts at: 2491 2662 2694 2950 2982 4143 4175 4305
Size: 171 32 256 32 1161 32 130
Fragments arranged by size:
1340 1161 711 329 256 171 130 32
32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 0 1586 2163 2312 3031 3665 3778 4305
Size: 1586 577 149 719 634 113 527
Fragments arranged by size:
1586 719 634 577 527 149 113
HindIII A'AGCT_T
Cuts at: 0 259 4305
Size: 259 4046
HpaI GTT'AAC
Cuts at: 0 2163 4305
Size: 2163 2142
KasI G'GCGC_C
Cuts at: 0 1823 4305
Size: 1823 2482
MfeI C'AATT_G
Cuts at: 0 4017 4305
Size: 4017 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 2720 4305
Size: 884 1836 1585
Fragments arranged by size:
1836 1585 884
MscI TGG'CCA
Cuts at: 0 2039 4305
Size: 2039 2266
MslI CAynn'nnrTG
Cuts at: 0 527 4104 4190 4305
Size: 527 3577 86 115
Fragments arranged by size:
3577 527 115 86
MspA1I CmG'CkG
Cuts at: 0 1378 1848 1965 2056 3093 4167 4305
Size: 1378 470 117 91 1037 1074 138
Fragments arranged by size:
1378 1074 1037 470 138 117 91
NarI GG'CG_CC
Cuts at: 0 1824 4305
Size: 1824 2481
NcoI C'CATG_G
Cuts at: 0 361 1664 4092 4305
Size: 361 1303 2428 213
Fragments arranged by size:
2428 1303 361 213
NdeI CA'TA_TG
Cuts at: 0 777 1688 2799 4305
Size: 777 911 1111 1506
Fragments arranged by size:
1506 1111 911 777
NsiI A_TGCA'T
Cuts at: 0 1683 4305
Size: 1683 2622
NspI r_CATG'y
Cuts at: 0 443 1016 1738 4178 4305
Size: 443 573 722 2440 127
Fragments arranged by size:
2440 722 573 443 127
PacI TTA_AT'TAA
Cuts at: 0 2441 4305
Size: 2441 1864
PflMI CCAn_nnn'nTGG
Cuts at: 0 2059 2836 4305
Size: 2059 777 1469
Fragments arranged by size:
2059 1469 777
PfoI T'CCnGG_A
Cuts at: 0 35 2574 4305
Size: 35 2539 1731
Fragments arranged by size:
2539 1731 35
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2203 2235 4305
Size: 2203 32 2070
Fragments arranged by size:
2203 2070 32
PpuMI rG'GwC_Cy
Cuts at: 0 2644 4305
Size: 2644 1661
PsiI TTA'TAA
Cuts at: 0 1648 2522 4199 4305
Size: 1648 874 1677 106
Fragments arranged by size:
1677 1648 874 106
PstI C_TGCA'G
Cuts at: 0 297 1569 3259 4166 4305
Size: 297 1272 1690 907 139
Fragments arranged by size:
1690 1272 907 297 139
PvuII CAG'CTG
Cuts at: 0 1378 1848 1965 3093 4167 4305
Size: 1378 470 117 1128 1074 138
Fragments arranged by size:
1378 1128 1074 470 138 117
SacI G_AGCT'C
Cuts at: 0 891 4305
Size: 891 3414
SapI GCTCTTCn'nnn_
Cuts at: 0 2576 4305
Size: 2576 1729
SfcI C'TryA_G
Cuts at: 0 293 1565 2765 3002 3255 3800 4162 4305
Size: 293 1272 1200 237 253 545 362 143
Fragments arranged by size:
1272 1200 545 362 293 253 237 143
SfoI GGC'GCC
Cuts at: 0 1825 4305
Size: 1825 2480
SmlI C'TyrA_G
Cuts at: 0 127 2137 2601 3894 4305
Size: 127 2010 464 1293 411
Fragments arranged by size:
2010 1293 464 411 127
SpeI A'CTAG_T
Cuts at: 0 2385 3334 4305
Size: 2385 949 971
Fragments arranged by size:
2385 971 949
SphI G_CATG'C
Cuts at: 0 1738 4178 4305
Size: 1738 2440 127
Fragments arranged by size:
2440 1738 127
SspI AAT'ATT
Cuts at: 0 1555 2596 3197 3717 4305
Size: 1555 1041 601 520 588
Fragments arranged by size:
1555 1041 601 588 520
StyI C'CwwG_G
Cuts at: 0 361 730 1385 1429 1664 4092 4203 4213
Size: 361 369 655 44 235 2428 111 10
Cuts at: 4213 4305
Size: 92
Fragments arranged by size:
2428 655 369 361 235 111 92 44
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 908 3218 4305
Size: 244 664 2310 1087
Fragments arranged by size:
2310 1087 664 244
TatI w'GTAC_w
Cuts at: 0 12 579 793 2974 4305
Size: 12 567 214 2181 1331
Fragments arranged by size:
2181 1331 567 214 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3071 3103 4073 4105 4305
Size: 3071 32 970 32 200
Fragments arranged by size:
3071 970 200 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 386 2595 3264 3865 4305
Size: 386 2209 669 601 440
Fragments arranged by size:
2209 669 601 440 386
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AlwNI ApaLI ApoI
AseI AvrII BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrGI BstAPI BstBI BstXI BstYI BtgI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PacI PflMI
PfoI PpiI PpuMI PsiI PstI PvuII SacI SapI
SfcI SfoI SmlI SpeI SphI SspI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaI AscI AsiSI AvaI BaeI BamHI BcgI
BglI BlpI BmgBI BmrI BmtI BsiEI BsiWI BsmI
BspMI BsrFI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI
EagI FseI FspI FspAI KpnI MluI NaeI NgoMIV
NheI NotI NruI PasI PciI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI