Restriction map for pF1KA1003
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AgeI        AhdI        AocI        ApoI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbvCI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BseAI       BsiEI       BspDI       BspEI       BspHI       
BsrDI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MluI        
MroI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RcaI        RsrII       SalI        SfuI        SgrAI       
SpeI        SspI        SwaI        Tth111I     XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI         1407  AACGTT               AA^CGTT
Alw44I       1854  GTGCAC               G^TGCAC
ApaLI        1854  GTGCAC               G^TGCAC
AseI         1951  ATTAAT               AT^TAAT
AsnI         1951  ATTAAT               AT^TAAT
AviII         539  TGCGCA               TGC^GCA
BbeI         1498  GGCGCC               GGCGC^C
BbsI         1615  GAAGAC               GAAGAC(2/6)
BfaI         1214  CTAG                 C^TAG
BpuAI        1615  GAAGAC               GAAGAC(2/6)
BsiWI        1256  CGTACG               C^GTACG
BsmI         1745  GCATTC               GAATGC(1/-1)
BspLU11I     1664  ACATGT               A^CATGT
BstYI        1736  AGATCC               [GA]^GATC[CT]
DraIII       1768  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1842  CCTTGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          539  TGCGCA               TGC^GCA
HincII        658  GTCAAC               GT[CT]^[GA]AC
HindII        658  GTCAAC               GT[CT]^[GA]AC
KasI         1498  GGCGCC               G^GCGCC
KspI         1079  CCGCGG               CCGC^GG
MaeI         1214  CTAG                 C^TAG
MfeI          492  CAATTG               C^AATTG
MflI         1736  AGATCC               [GA]^GATC[CT]
MunI          492  CAATTG               C^AATTG
NaeI         1485  GCCGGC               GCC^GGC
NarI         1498  GGCGCC               GG^CGCC
NcoI         1058  CCATGG               C^CATGG
NgoMIV       1485  GCCGGC               G^CCGGC
PshBI        1951  ATTAAT               AT^TAAT
Psp1406I     1407  AACGTT               AA^CGTT
SacI          736  GAGCTC               GAGCT^C
SacII        1079  CCGCGG               CCGC^GG
SapI         2046  GAAGAGC              GCTCTTC(1/4)
SbfI          477  CCTGCAGG             CCTGCA^GG
ScaI         1709  AGTACT               AGT^ACT
SexAI        2249  ACCTGGT              A^CC[AT]GGT
SfiI         2415  GGCCTTCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1498  GGCGCC               GGC^GCC
SmaI         2515  CCCGGG               CCC^GGG
SnaBI        2029  TACGTA               TAC^GTA
SphI         2459  GCATGC               GCATG^C
Sse8387I      477  CCTGCAGG             CCTGCA^GG
Tsp509I       493  AATT                 ^AATT
XcmI         2339  CCATCTGCCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1736  AGATCC               [GA]^GATC[CT]
XmaCI        2515  CCCGGG               C^CCGGG
XmaI         2515  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Aor51HI      1313  AGCGCT               AGC^GCT
             1757  AGCGCT              
ApaI         2518  GGGCCC               GGGCC^C
             2685  GGGCCC              
BanI         1248  GGCACC               G^G[CT][GA]CC
             1498  GGCGCC              
BcgI         1194  CGAGGGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2375  GCAGGAGGATCG        
BciVI        1390  GTATCC               GTATCC(6/5)
             1570  GTATCC              
BsaBI        2382  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2652  GATTGCCATC          
BsaI         2608  GAGACC               GGTCTC(1/5)
              585  GGTCTC              
BsaWI         106  ACCGGA               [AT]^CCGG[AT]
              824  ACCGGA              
Bsp1407I     1667  TGTACA               T^GTACA
             2288  TGTACA              
BsrBI         973  GAGCGG               CCGCTC(-3/-3)
             1678  GAGCGG              
BsrFI         747  GCCGGT               [GA]^CCGG[CT]
             1485  GCCGGC              
BsrGI        1667  TGTACA               T^GTACA
             2288  TGTACA              
BstXI         112  CCAAACCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1394  CCACCACAGTGG        
Cfr10I        747  GCCGGT               [GA]^CCGG[CT]
             1485  GCCGGC              
DrdI         1978  GACCAGTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2248  GACCTGGTGGTC        
Eco47III     1313  AGCGCT               AGC^GCT
             1757  AGCGCT              
EcoT14I      1058  CCATGG               C^C[AT][AT]GG
             1847  CCAAGG              
MamI         2382  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2652  GATTGCCATC          
MseI         1651  TTAA                 T^TAA
             1952  TTAA                
NspI         1664  ACATGT               [GA]CATG^[CT]
             2459  GCATGC              
PflMI         576  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2664  CCAGAGTGTGG         
PpuMI          14  GGGACCT              [GA]G^G[AT]CC[CT]
             1445  AGGACCT             
SspBI        1667  TGTACA               T^GTACA
             2288  TGTACA              
StuI          426  AGGCCT               AGG^CCT
             2414  AGGCCT              
StyI         1058  CCATGG               C^C[AT][AT]GG
             1847  CCAAGG              
Tru9I        1651  TTAA                 T^TAA
             1952  TTAA                
Van91I        576  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2664  CCAGAGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BalI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BssSI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PaeR7I      
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     XhoI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com