Restriction map for pF1KA1003
 (Linear) (Six-base) MAPSORT of: KIAA1003.seq  Check: 9608  from: 1  to: 2739



KIAA1003.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2070    2739 
   Size:       2070     669
 
AclI AA'CG_TT

Cuts at:      0    1408    2739 
   Size:       1408    1331
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2593    2739 
   Size:       2593     146
 
AfeI AGC'GCT

Cuts at:      0    1315    1759    2739 
   Size:       1315     444     980

  Fragments arranged by size: 

                1315     980     444
 
AflIII A'CryG_T

Cuts at:      0    1664    2129    2330    2739 
   Size:       1664     465     201     409

  Fragments arranged by size: 

                1664     465     409     201
 
AleI CACnn'nnGTG

Cuts at:      0    1399    2739 
   Size:       1399    1340
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1738    2476    2739 
   Size:       1738     738     263

  Fragments arranged by size: 

                1738     738     263
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     976    1008    2739 
   Size:        976      32    1731

  Fragments arranged by size: 

                1731     976      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     285     486     582    1118    2682    2739 
   Size:        285     201      96     536    1564      57

  Fragments arranged by size: 

                1564     536     285     201      96      57
 
ApaI G_GGCC'C

Cuts at:      0    2522    2689    2739 
   Size:       2522     167      50

  Fragments arranged by size: 

                2522     167      50
 
ApaLI G'TGCA_C

Cuts at:      0    1854    2739 
   Size:       1854     885
 
AseI AT'TA_AT

Cuts at:      0    1952    2739 
   Size:       1952     787
 
AvaI C'yCGr_G

Cuts at:      0     422     448     779     861    1732    1816    2515    2647 
   Size:        422      26     331      82     871      84     699     132

Cuts at:   2647    2739 
   Size:         92

  Fragments arranged by size: 

                 871     699     422     331     132      92      84      82
                  26
 
BanI G'GyrC_C

Cuts at:      0    1248    1498    2739 
   Size:       1248     250    1241

  Fragments arranged by size: 

                1248    1241     250
 
BanII G_rGCy'C

Cuts at:      0     307     740     832    1826    2111    2513    2522    2689 
   Size:        307     433      92     994     285     402       9     167

Cuts at:   2689    2739 
   Size:         50

  Fragments arranged by size: 

                 994     433     402     307     285     167      92      50
                   9
 
BbeI G_GCGC'C

Cuts at:      0    1502    2739 
   Size:       1502    1237
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1622    2739 
   Size:       1622    1117
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1183    1217    2364    2398    2739 
   Size:       1183      34    1147      34     341

  Fragments arranged by size: 

                1183    1147     341      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1401    1581    2739 
   Size:       1401     180    1158

  Fragments arranged by size: 

                1401    1158     180
 
BglI GCCn_nnn'nGGC

Cuts at:      0     434     481     763    1904    2422    2517    2739 
   Size:        434      47     282    1141     518      95     222

  Fragments arranged by size: 

                1141     518     434     282     222      95      47
 
Bme1580I G_kGCm'C

Cuts at:      0    1858    1925    2522    2689    2739 
   Size:       1858      67     597     167      50

  Fragments arranged by size: 

                1858     597     167      67      50
 
BmgBI CAC'GTC

Cuts at:      0    1803    2739 
   Size:       1803     936
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1095    2739 
   Size:        295     800    1644

  Fragments arranged by size: 

                1644     800     295
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1249    2739 
   Size:        724     525    1490

  Fragments arranged by size: 

                1490     724     525
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     591    2602    2739 
   Size:        591    2011     137

  Fragments arranged by size: 

                2011     591     137
 
BsaAI yAC'GTr

Cuts at:      0     621    1260    2031    2130    2331    2739 
   Size:        621     639     771      99     201     408

  Fragments arranged by size: 

                 771     639     621     408     201      99
 
BsaBI GATnn'nnATC

Cuts at:      0    2386    2656    2739 
   Size:       2386     270      83

  Fragments arranged by size: 

                2386     270      83
 
BsaHI Gr'CG_yC

Cuts at:      0    1126    1499    2441    2739 
   Size:       1126     373     942     298

  Fragments arranged by size: 

                1126     942     373     298
 
BsaWI w'CCGG_w

Cuts at:      0     106     824    2739 
   Size:        106     718    1915

  Fragments arranged by size: 

                1915     718     106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      49    2272    2302    2320    2350    2739 
   Size:         19      30    2223      30      18      30     389

  Fragments arranged by size: 

                2223     389      30      30      30      19      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     297     748    1152    1873    2071    2443    2620    2739 
   Size:        297     451     404     721     198     372     177     119

  Fragments arranged by size: 

                 721     451     404     372     297     198     177     119
            
 
BseYI C'CCAG_C

Cuts at:      0     300     683    1088    1582    2110    2682    2739 
   Size:        300     383     405     494     528     572      57

  Fragments arranged by size: 

                 572     528     494     405     383     300      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1387    2032    2683    2739 
   Size:        712     675     645     651      56

  Fragments arranged by size: 

                 712     675     651     645      56
 
BsiHKAI G_wGCw'C

Cuts at:      0     740    1265    1858    2285    2338    2739 
   Size:        740     525     593     427      53     401

  Fragments arranged by size: 

                 740     593     525     427     401      53
 
BsiWI C'GTAC_G

Cuts at:      0    1256    2739 
   Size:       1256    1483
 
BsmI GAATG_Cn'

Cuts at:      0    1745    2739 
   Size:       1745     994
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     916    1809    1996    2719    2739 
   Size:        916     893     187     723      20

  Fragments arranged by size: 

                 916     893     723     187      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     307     740     832    1265    1826    1858    1925    2111 
   Size:        307     433      92     433     561      32      67     186

Cuts at:   2111    2285    2338    2513    2522    2689    2739 
   Size:        174      53     175       9     167      50

  Fragments arranged by size: 

                 561     433     433     307     186     175     174     167
                  92      67      53      50      32       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     123    1838    2070    2705    2739 
   Size:        123    1715     232     635      34

  Fragments arranged by size: 

                1715     635     232     123      34
 
BsrBI CCG'CTC

Cuts at:      0     975    1680    2739 
   Size:        975     705    1059

  Fragments arranged by size: 

                1059     975     705
 
BsrFI r'CCGG_y

Cuts at:      0     747    1485    2739 
   Size:        747     738    1254

  Fragments arranged by size: 

                1254     747     738
 
BsrGI T'GTAC_A

Cuts at:      0    1667    2288    2739 
   Size:       1667     621     451

  Fragments arranged by size: 

                1667     621     451
 
BssSI C'ACGA_G

Cuts at:      0     730    1192    1226    1363    1768    1999    2739 
   Size:        730     462      34     137     405     231     740

  Fragments arranged by size: 

                 740     730     462     405     231     137      34
 
BstEII G'GTnAC_C

Cuts at:      0      48     102     820    2739 
   Size:         48      54     718    1919

  Fragments arranged by size: 

                1919     718      54      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     119    1401    2739 
   Size:        119    1282    1338

  Fragments arranged by size: 

                1338    1282     119
 
BstYI r'GATC_y

Cuts at:      0    1736    2739 
   Size:       1736    1003
 
BtgI C'CryG_G

Cuts at:      0     758    1058    1079    1102    1902    2208    2739 
   Size:        758     300      21      23     800     306     531

  Fragments arranged by size: 

                 800     758     531     306     300      23      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1914    2739 
   Size:       1914     825
 
BtsI GCAGTG_nn'

Cuts at:      0     873    1052    1375    1423    1923    1966    2470    2739 
   Size:        873     179     323      48     500      43     504     269

  Fragments arranged by size: 

                 873     504     500     323     269     179      48      43
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     602     637    2739 
   Size:        602      35    2102

  Fragments arranged by size: 

                2102     602      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1773    2739 
   Size:       1773     966
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1984    2254    2739 
   Size:       1984     270     485

  Fragments arranged by size: 

                1984     485     270
 
EaeI y'GGCC_r

Cuts at:      0     610     755    1451    1487    2093    2366    2559    2739 
   Size:        610     145     696      36     606     273     193     180

  Fragments arranged by size: 

                 696     610     606     273     193     180     145      36
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     315    2041    2739 
   Size:        315    1726     698

  Fragments arranged by size: 

                1726     698     315
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1317    2650    2739 
   Size:       1317    1333      89

  Fragments arranged by size: 

                1333    1317      89
 
EcoICRI GAG'CTC

Cuts at:      0     738    2739 
   Size:        738    2001
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1095    2593    2739 
   Size:        295     800    1498     146

  Fragments arranged by size: 

                1498     800     295     146
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1846    2739 
   Size:       1846     893
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     483    1446    1597    2176    2739 
   Size:         15     468     963     151     579     563

  Fragments arranged by size: 

                 963     579     563     468     151      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228     721    1639    2739 
   Size:        228     493     918    1100

  Fragments arranged by size: 

                1100     918     493     228
 
FspI TGC'GCA

Cuts at:      0     541    2739 
   Size:        541    2198
 
FspAI rTGC'GCAy

Cuts at:      0     541    2739 
   Size:        541    2198
 
HaeII r_GCGC'y

Cuts at:      0     769    1317    1502    1761    2739 
   Size:        769     548     185     259     978

  Fragments arranged by size: 

                 978     769     548     259     185
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     382     414     762     794     835     867    1375    1407 
   Size:        382      32     348      32      41      32     508      32

Cuts at:   1407    1843    1870    1875    1902    2739 
   Size:        436      27       5      27     837

  Fragments arranged by size: 

                 837     508     436     382     348      41      32      32
                  32      32      27      27       5
 
HincII GTy'rAC

Cuts at:      0     660    2739 
   Size:        660    2079
 
KasI G'GCGC_C

Cuts at:      0    1498    2739 
   Size:       1498    1241
 
MfeI C'AATT_G

Cuts at:      0     492    2739 
   Size:        492    2247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     261    1581    2739 
   Size:        261    1320    1158

  Fragments arranged by size: 

                1320    1158     261
 
MscI TGG'CCA

Cuts at:      0     757    1453    2368    2739 
   Size:        757     696     915     371

  Fragments arranged by size: 

                 915     757     696     371
 
MslI CAynn'nnrTG

Cuts at:      0     436    1057    1399    2458    2739 
   Size:        436     621     342    1059     281

  Fragments arranged by size: 

                1059     621     436     342     281
 
MspA1I CmG'CkG

Cuts at:      0     282     504     726    1081    1586    1642    1673    2571 
   Size:        282     222     222     355     505      56      31     898

Cuts at:   2571    2682    2739 
   Size:        111      57

  Fragments arranged by size: 

                 898     505     355     282     222     222     111      57
                  56      31
 
NaeI GCC'GGC

Cuts at:      0    1487    2739 
   Size:       1487    1252
 
NarI GG'CG_CC

Cuts at:      0    1499    2739 
   Size:       1499    1240
 
NcoI C'CATG_G

Cuts at:      0    1058    2739 
   Size:       1058    1681
 
NgoMIV G'CCGG_C

Cuts at:      0    1485    2739 
   Size:       1485    1254
 
NspI r_CATG'y

Cuts at:      0    1668    2463    2739 
   Size:       1668     795     276

  Fragments arranged by size: 

                1668     795     276
 
PasI CC'CwG_GG

Cuts at:      0    1524    2739 
   Size:       1524    1215
 
PciI A'CATG_T

Cuts at:      0    1664    2739 
   Size:       1664    1075
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     582    2670    2739 
   Size:        582    2088      69

  Fragments arranged by size: 

                2088     582      69
 
PfoI T'CCnGG_A

Cuts at:      0     272    1412    1434    2471    2739 
   Size:        272    1140      22    1037     268

  Fragments arranged by size: 

                1140    1037     272     268      22
 
PpuMI rG'GwC_Cy

Cuts at:      0      15    1446    2739 
   Size:         15    1431    1293

  Fragments arranged by size: 

                1431    1293      15
 
PspOMI G'GGCC_C

Cuts at:      0    2518    2685    2739 
   Size:       2518     167      54

  Fragments arranged by size: 

                2518     167      54
 
PspXI vC'TCGA_Gb

Cuts at:      0     422     861    1816    2739 
   Size:        422     439     955     923

  Fragments arranged by size: 

                 955     923     439     422
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     445     477    2311    2343    2739 
   Size:        445      32    1834      32     396

  Fragments arranged by size: 

                1834     445     396      32      32
 
PstI C_TGCA'G

Cuts at:      0     134     482    1177    2739 
   Size:        134     348     695    1562

  Fragments arranged by size: 

                1562     695     348     134
 
PvuII CAG'CTG

Cuts at:      0     726    1586    1673    2571    2739 
   Size:        726     860      87     898     168

  Fragments arranged by size: 

                 898     860     726     168      87
 
SacI G_AGCT'C

Cuts at:      0     740    2739 
   Size:        740    1999
 
SacII CC_GC'GG

Cuts at:      0    1082    2739 
   Size:       1082    1657
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2041    2739 
   Size:       2041     698
 
SbfI CC_TGCA'GG

Cuts at:      0     482    2739 
   Size:        482    2257
 
ScaI AGT'ACT

Cuts at:      0    1711    2739 
   Size:       1711    1028
 
SexAI A'CCwGG_T

Cuts at:      0    2249    2739 
   Size:       2249     490
 
SfcI C'TryA_G

Cuts at:      0     130     478    1173    2040    2739 
   Size:        130     348     695     867     699

  Fragments arranged by size: 

                 867     699     695     348     130
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2422    2739 
   Size:       2422     317
 
SfoI GGC'GCC

Cuts at:      0    1500    2739 
   Size:       1500    1239
 
SmaI CCC'GGG

Cuts at:      0    2517    2739 
   Size:       2517     222
 
SmlI C'TyrA_G

Cuts at:      0     422     739     861    1264    1816    2739 
   Size:        422     317     122     403     552     923

  Fragments arranged by size: 

                 923     552     422     403     317     122
 
SnaBI TAC'GTA

Cuts at:      0    2031    2739 
   Size:       2031     708
 
SphI G_CATG'C

Cuts at:      0    2463    2739 
   Size:       2463     276
 
StuI AGG'CCT

Cuts at:      0     428    2416    2739 
   Size:        428    1988     323

  Fragments arranged by size: 

                1988     428     323
 
StyI C'CwwG_G

Cuts at:      0    1058    1847    2739 
   Size:       1058     789     892

  Fragments arranged by size: 

                1058     892     789
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     709    1878    2739 
   Size:        709    1169     861

  Fragments arranged by size: 

                1169     861     709
 
TatI w'GTAC_w

Cuts at:      0    1550    1667    1709    1778    2288    2739 
   Size:       1550     117      42      69     510     451

  Fragments arranged by size: 

                1550     510     451     117      69      42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      19      51    2739 
   Size:         19      32    2688

  Fragments arranged by size: 

                2688      32      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2346    2739 
   Size:       2346     393
 
XhoI C'TCGA_G

Cuts at:      0     422     861    1816    2739 
   Size:        422     439     955     923

  Fragments arranged by size: 

                 955     923     439     422
 
XmaI C'CCGG_G

Cuts at:      0    2515    2739 
   Size:       2515     224
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AseI     AvaI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BglI Bme1580I    BmgBI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
    PpuMI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI    SacII
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI
     SmlI    SnaBI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I    AflII     AgeI     AhdI     ApoI     AscI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI     BplI   Bpu10I    BsiEI    BspEI    BspHI
    BsrDI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI     DraI
     EagI    EcoRI    EcoRV     FseI  HindIII     HpaI     KpnI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PpiI    PshAI     PsiI     PvuI    RsrII     SalI    SanDI     SgfI
    SgrAI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XmnI
     ZraI
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