################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccI AccII AclI AflII AflIII AgeI Aor51HI ApaI AscI Asp700I AviII AvrII BbrPI BbsI BcgI BciVI BfrI BglI BlnI BpuAI BsaAI BsaBI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FseI FspI HgaI HindIII HpaI KspI MamI MfeI MluI MunI MvnI NaeI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SgrAI SmaI SnaBI Sse8387I SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 1530 GGTACC G^GTACC AccIII 602 TCCGGA T^CCGGA AcyI 2272 GGCGCC G[GA]^CG[CT]C AhdI 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2089 GTGCAC G^TGCAC ApaLI 2089 GTGCAC G^TGCAC AseI 139 ATTAAT AT^TAAT AsnI 139 ATTAAT AT^TAAT Asp718I 1530 GGTACC G^GTACC AspEI 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2089 GTGCAC G[AT]GC[AT]^C AspI 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 662 CCCGAG C^[CT]CG[GA]G BamHI 2495 GGATCC G^GATCC BanII 1578 GAGCCC G[GA]GC[CT]^C BbeI 2272 GGCGCC GGCGC^C BclI 243 TGATCA T^GATCA BglII 2208 AGATCT A^GATCT BsaHI 2272 GGCGCC G[GA]^CG[CT]C BsaI 1820 GAGACC GGTCTC(1/5) BseAI 602 TCCGGA T^CCGGA BseRI 1359 GAGGAG GAGGAG(10/8) BsgI 1935 CTGCAC GTGCAG(16/14) BsiHKAI 2089 GTGCAC G[AT]GC[AT]^C BsmBI 2434 CGTCTC CGTCTC(1/5) BsoBI 662 CCCGAG C^[CT]CG[GA]G BspEI 602 TCCGGA T^CCGGA BspMI 1557 ACCTGC ACCTGC(4/8) BstBI 2465 TTCGAA TT^CGAA BstEII 756 GGTCACC G^GT[ACGT]ACC BstPI 756 GGTCACC G^GT[ACGT]ACC DraII 1340 AGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2248 CTCTTC CTCTTC(1/4) EcoNI 1582 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1340 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 756 GGTCACC G^GT[ACGT]ACC EcoRV 416 GATATC GAT^ATC FbaI 243 TGATCA T^GATCA Hin1I 2272 GGCGCC G[GA]^CG[CT]C HincII 2076 GTCAAC GT[CT]^[GA]AC HindII 2076 GTCAAC GT[CT]^[GA]AC KasI 2272 GGCGCC G^GCGCC KpnI 1530 GGTACC GGTAC^C Ksp632I 2248 CTCTTC CTCTTC(1/4) MaeII 1227 ACGT A^CGT MroI 602 TCCGGA T^CCGGA MseI 140 TTAA T^TAA NarI 2272 GGCGCC GG^CGCC NheI 631 GCTAGC G^CTAGC NspV 2465 TTCGAA TT^CGAA PflFI 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 139 ATTAAT AT^TAAT ScaI 1673 AGTACT AGT^ACT SfoI 2272 GGCGCC GGC^GCC SfuI 2465 TTCGAA TT^CGAA SpeI 277 ACTAGT A^CTAGT SphI 608 GCATGC GCATG^C Tru9I 140 TTAA T^TAA Tth111I 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AocI 2215 CCTGAGG CC^T[ACGT]AGG 2490 CCTGAGG BlpI 1347 GCTCAGC GC^T[ACGT]AGC 1561 GCTCAGC Bpu1102I 1347 GCTCAGC GC^T[ACGT]AGC 1561 GCTCAGC BsaWI 191 ACCGGA [AT]^CCGG[AT] 602 TCCGGA Bsp1407I 1826 TGTACA T^GTACA 2378 TGTACA BsrGI 1826 TGTACA T^GTACA 2378 TGTACA Bsu36I 2215 CCTGAGG CC^T[ACGT]AGG 2490 CCTGAGG CelII 1347 GCTCAGC GC^T[ACGT]AGC 1561 GCTCAGC CfoI 2007 GCGC GCG^C 2273 GCGC Eco81I 2215 CCTGAGG CC^T[ACGT]AGG 2490 CCTGAGG HaeII 2006 GGCGCT [GA]GCGC^[CT] 2272 GGCGCC HhaI 2007 GCGC GCG^C 2273 GCGC HinP1I 2007 GCGC G^CGC 2273 GCGC MslI 1857 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2382 CACCATTGTG NcoI 1760 CCATGG C^CATGG 2067 CCATGG NspI 608 GCATGC [GA]CATG^[CT] 1118 ACATGC PstI 968 CTGCAG CTGCA^G 1647 CTGCAG SexAI 752 ACCAGGT A^CC[AT]GGT 1698 ACCAGGT SspBI 1826 TGTACA T^GTACA 2378 TGTACA StuI 1386 AGGCCT AGG^CCT 1512 AGGCCT TaqI 1191 TCGA T^CGA 2466 TCGA TthHB8I 1191 TCGA T^CGA 2466 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BalI BanI BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraIII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI XcmI XhoII