Restriction map for pF1KA0996
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BfrI        BglII       BlnI        BsaI        
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspLU11I    BsrFI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
FseI        FspI        HincII      HindII      HpaI        
KasI        KpnI        MfeI        MluI        MluNI       
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SbfI        SfoI        SgrAI       
SmaI        SnaBI       Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII          88  GACGTC               GACGT^C
AccI           52  GTCTAC               GT^[AC][GT]AC
AccIII        716  TCCGGA               T^CCGGA
AcyI           88  GACGTC               G[GA]^CG[CT]C
AlwI         1939  GATCC                GGATC(4/5)
AlwNI         559  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2446  CCTAAGG              CC^T[ACGT]AGG
AseI         1615  ATTAAT               AT^TAAT
AsnI         1615  ATTAAT               AT^TAAT
AvaI         1127  CTCGAG               C^[CT]CG[GA]G
BbvCI           7  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1426  GCATTCTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1809  GGATAC               GTATCC(6/5)
BclI         1644  TGATCA               T^GATCA
BglI          121  GCCTCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          213  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      213  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1628  TACGTG               [CT]AC^GT[GA]
BsaBI        2256  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          88  GACGTC               G[GA]^CG[CT]C
BsaWI         716  TCCGGA               [AT]^CCGG[AT]
BseAI         716  TCCGGA               T^CCGGA
BsoBI        1127  CTCGAG               C^[CT]CG[GA]G
BspEI         716  TCCGGA               T^CCGGA
BspHI         278  TCATGA               T^CATGA
BspMI        2388  ACCTGC               ACCTGC(4/8)
BsrDI        1229  GCAATG               GCAATG(2/0)
BssSI        1039  CACGAG               CACGAG(-5/-1)
BstAPI        778  GCACACCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         221  CCATCGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1938  AGATCC               [GA]^GATC[CT]
Bsu36I       2446  CCTAAGG              CC^T[ACGT]AGG
CelII         213  GCTGAGC              GC^T[ACGT]AGC
EaeI           98  TGGCCG               [CT]^GGCC[GA]
Eco81I       2446  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1551  CCTCACTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2343  GATATC               GAT^ATC
EcoT22I      1516  ATGCAT               ATGCA^T
FbaI         1644  TGATCA               T^GATCA
HaeII         217  AGCGCC               [GA]GCGC^[CT]
Hin1I          88  GACGTC               G[GA]^CG[CT]C
MamI         2256  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1938  AGATCC               [GA]^GATC[CT]
MroI          716  TCCGGA               T^CCGGA
NcoI          125  CCATGG               C^CATGG
NsiI         1516  ATGCAT               ATGCA^T
PaeR7I       1127  CTCGAG               C^TCGAG
PflMI        2230  CCAGTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1615  ATTAAT               AT^TAAT
RcaI          278  TCATGA               T^CATGA
SexAI         757  ACCAGGT              A^CC[AT]GGT
SfiI          120  GGCCTCCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          888  ACTAGT               A^CTAGT
SphI           32  GCATGC               GCATG^C
StuI          614  AGGCCT               AGG^CCT
Van91I       2230  CCAGTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1127  CTCGAG               C^TCGAG
XhoII        1938  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI           82  GGGCCC               GGGCC^C
              157  GGGCCC              
Asp700I      1952  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2464  GAAGGACTTC          
AvaII         339  GGACC                G^G[AT]CC
             2111  GGACC               
BanII          82  GGGCCC               G[GA]GC[CT]^C
              157  GGGCCC              
BcnI          342  CCCGG                CC^[GC]GG
             2019  CCGGG               
BsmAI        1269  GAGAC                GTCTC(1/5)
             1305  GAGAC               
BsmI         1365  GAATGC               GAATGC(1/-1)
             1426  GCATTC              
BsrBI          61  CCGCTC               CCGCTC(-3/-3)
              439  GAGCGG              
BstBI         948  TTCGAA               TT^CGAA
             1196  TTCGAA              
DraI          934  TTTAAA               TTT^AAA
             1045  TTTAAA              
EcoRI         954  GAATTC               G^AATTC
             1729  GAATTC              
HindIII       629  AAGCTT               A^AGCTT
             1529  AAGCTT              
KspI          110  CCGCGG               CCGC^GG
              184  CCGCGG              
NciI          342  CCCGG                CC^[GC]GG
             2019  CCGGG               
NspI           32  GCATGC               [GA]CATG^[CT]
               48  GCATGT              
NspV          948  TTCGAA               TT^CGAA
             1196  TTCGAA              
PleI         1271  GACTC                GAGTC(4/5)
             2162  GAGTC               
PstI          265  CTGCAG               CTGCA^G
              748  CTGCAG              
SacII         110  CCGCGG               CCGC^GG
              184  CCGCGG              
SapI          742  GAAGAGC              GCTCTTC(1/4)
             1255  GAAGAGC             
ScaI          386  AGTACT               AGT^ACT
             1659  AGTACT              
SfaNI        1033  GATGC                GCATC(5/9)
             2029  GCATC               
SfcI          265  CTGCAG               C^T[GA][CT]AG
              748  CTGCAG              
SfuI          948  TTCGAA               TT^CGAA
             1196  TTCGAA              
SmlI          517  CTCAAG               C^T[CT][GA]AG
             1127  CTCGAG              
XmnI         1952  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2464  GAAGGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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