Restriction map for pF1KA0996
 (Linear) (Six-base) MAPSORT of: KIAA0996.seq  Check: 919  from: 1  to: 2544



KIAA0996.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2397    2544 
   Size:       2397     147
 
AatII G_ACGT'C

Cuts at:      0      92    2544 
   Size:         92    2452
 
AccI GT'mk_AC

Cuts at:      0      53    2544 
   Size:         53    2491
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     686    1766    2519    2544 
   Size:        373     313    1080     753      25

  Fragments arranged by size: 

                1080     753     373     313      25
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     499    2544 
   Size:        499    2045
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564    2544 
   Size:        564    1980
 
ApaI G_GGCC'C

Cuts at:      0      86     161    2544 
   Size:         86      75    2383

  Fragments arranged by size: 

                2383      86      75
 
ApoI r'AATT_y

Cuts at:      0     672     931     954    1721    1729    2544 
   Size:        672     259      23     767       8     815

  Fragments arranged by size: 

                 815     767     672     259      23       8
 
AseI AT'TA_AT

Cuts at:      0    1616    2544 
   Size:       1616     928
 
AvaI C'yCGr_G

Cuts at:      0    1127    2544 
   Size:       1127    1417
 
BanII G_rGCy'C

Cuts at:      0      86     161    2544 
   Size:         86      75    2383

  Fragments arranged by size: 

                2383      86      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     311     845    1058    1949    2318    2544 
   Size:        311     534     213     891     369     226

  Fragments arranged by size: 

                 891     534     369     311     226     213
 
BbvCI CC'TCA_GC

Cuts at:      0       8    2544 
   Size:          8    2536
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1415    1449    2544 
   Size:       1415      34    1095

  Fragments arranged by size: 

                1415    1095      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1803    2544 
   Size:       1803     741
 
BclI T'GATC_A

Cuts at:      0    1644    2544 
   Size:       1644     900
 
BfrBI ATG'CAT

Cuts at:      0    1518    2544 
   Size:       1518    1026
 
BglI GCCn_nnn'nGGC

Cuts at:      0     127    2544 
   Size:        127    2417
 
BlpI GC'TnA_GC

Cuts at:      0     214    2544 
   Size:        214    2330
 
Bme1580I G_kGCm'C

Cuts at:      0      86     161     319    2544 
   Size:         86      75     158    2225

  Fragments arranged by size: 

                2225     158      86      75
 
BmgBI CAC'GTC

Cuts at:      0     228     255    2544 
   Size:        228      27    2289

  Fragments arranged by size: 

                2289     228      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2325    2544 
   Size:       2325     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     454    1258    1844    2544 
   Size:        211     243     804     586     700

  Fragments arranged by size: 

                 804     700     586     243     211
 
Bpu10I CC'TnA_GC

Cuts at:      0       8     451    2544 
   Size:          8     443    2093

  Fragments arranged by size: 

                2093     443       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502    2544 
   Size:        502    2042
 
BsaAI yAC'GTr

Cuts at:      0    1630    2544 
   Size:       1630     914
 
BsaBI GATnn'nnATC

Cuts at:      0    2260    2544 
   Size:       2260     284
 
BsaHI Gr'CG_yC

Cuts at:      0      89    2544 
   Size:         89    2455
 
BsaWI w'CCGG_w

Cuts at:      0     716    2544 
   Size:        716    1828
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1959    1989    2153    2183    2544 
   Size:       1959      30     164      30     361

  Fragments arranged by size: 

                1959     361     164      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451    1536    1777    2005    2008    2544 
   Size:        451    1085     241     228       3     536

  Fragments arranged by size: 

                1085     536     451     241     228       3
 
BseYI C'CCAG_C

Cuts at:      0     142     557    1371    2544 
   Size:        142     415     814    1173

  Fragments arranged by size: 

                1173     814     415     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     409     761     812    1363    1812    2544 
   Size:        379      30     352      51     551     449     732

  Fragments arranged by size: 

                 732     551     449     379     352      51      30
 
BsmI GAATG_Cn'

Cuts at:      0    1371    1426    2544 
   Size:       1371      55    1118

  Fragments arranged by size: 

                1371    1118      55
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     161     319    2544 
   Size:         86      75     158    2225

  Fragments arranged by size: 

                2225     158      86      75
 
BspEI T'CCGG_A

Cuts at:      0     716    2544 
   Size:        716    1828
 
BspHI T'CATG_A

Cuts at:      0     278    2544 
   Size:        278    2266
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2397    2544 
   Size:       2397     147
 
BsrBI CCG'CTC

Cuts at:      0      63     441    2544 
   Size:         63     378    2103

  Fragments arranged by size: 

                2103     378      63
 
BsrDI GCAATG_nn'

Cuts at:      0    1236    2544 
   Size:       1236    1308
 
BssSI C'ACGA_G

Cuts at:      0    1039    2544 
   Size:       1039    1505
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     784    2544 
   Size:        784    1760
 
BstBI TT'CG_AA

Cuts at:      0     949    1197    2544 
   Size:        949     248    1347

  Fragments arranged by size: 

                1347     949     248
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     228    2544 
   Size:        228    2316
 
BstYI r'GATC_y

Cuts at:      0    1938    2544 
   Size:       1938     606
 
Bsu36I CC'TnA_GG

Cuts at:      0    2447    2544 
   Size:       2447      97
 
BtgI C'CryG_G

Cuts at:      0     110     125     184    2544 
   Size:        110      15      59    2360

  Fragments arranged by size: 

                2360     110      59      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2194    2544 
   Size:       2194     350
 
BtsI GCAGTG_nn'

Cuts at:      0     321     456    1800    2544 
   Size:        321     135    1344     744

  Fragments arranged by size: 

                1344     744     321     135
 
DraI TTT'AAA

Cuts at:      0     936    1047    2544 
   Size:        936     111    1497

  Fragments arranged by size: 

                1497     936     111
 
EaeI y'GGCC_r

Cuts at:      0      98    2544 
   Size:         98    2446
 
EarI CTCTTCn'nnn_

Cuts at:      0     518     737    1250    1531    1785    2333    2544 
   Size:        518     219     513     281     254     548     211

  Fragments arranged by size: 

                 548     518     513     281     254     219     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     373     454     686    1258    1766    1844    2519 
   Size:        211     162      81     232     572     508      78     675

Cuts at:   2519    2544 
   Size:         25

  Fragments arranged by size: 

                 675     572     508     232     211     162      81      78
                  25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1555    2544 
   Size:       1555     989
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82     157     158    2544 
   Size:         82      75       1    2386

  Fragments arranged by size: 

                2386      82      75       1
 
EcoRI G'AATT_C

Cuts at:      0     954    1729    2544 
   Size:        954     775     815

  Fragments arranged by size: 

                 954     815     775
 
EcoRV GAT'ATC

Cuts at:      0    2345    2544 
   Size:       2345     199
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1547    2091    2544 
   Size:       1547     544     453

  Fragments arranged by size: 

                1547     544     453
 
HaeII r_GCGC'y

Cuts at:      0     221    2544 
   Size:        221    2323
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1200    1232    1284    1316    1888    1920    2311    2343 
   Size:       1200      32      52      32     572      32     391      32

Cuts at:   2343    2544 
   Size:        201

  Fragments arranged by size: 

                1200     572     391     201      52      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     629    1529    2544 
   Size:        629     900    1015

  Fragments arranged by size: 

                1015     900     629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    2544 
   Size:       1037    1507
 
MslI CAynn'nnrTG

Cuts at:      0     226     605     788    2544 
   Size:        226     379     183    1756

  Fragments arranged by size: 

                1756     379     226     183
 
MspA1I CmG'CkG

Cuts at:      0      16      71     112     186     423     564    1751    2544 
   Size:         16      55      41      74     237     141    1187     793

  Fragments arranged by size: 

                1187     793     237     141      74      55      41      16
            
 
NcoI C'CATG_G

Cuts at:      0     125    2544 
   Size:        125    2419
 
NsiI A_TGCA'T

Cuts at:      0    1520    2544 
   Size:       1520    1024
 
NspI r_CATG'y

Cuts at:      0      36      52    2544 
   Size:         36      16    2492

  Fragments arranged by size: 

                2492      36      16
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2236    2544 
   Size:       2236     308
 
PfoI T'CCnGG_A

Cuts at:      0    1274    2544 
   Size:       1274    1270
 
PsiI TTA'TAA

Cuts at:      0    1297    2544 
   Size:       1297    1247
 
PspOMI G'GGCC_C

Cuts at:      0      82     157    2544 
   Size:         82      75    2387

  Fragments arranged by size: 

                2387      82      75
 
PspXI vC'TCGA_Gb

Cuts at:      0    1127    2544 
   Size:       1127    1417
 
PstI C_TGCA'G

Cuts at:      0     269     752    2544 
   Size:        269     483    1792

  Fragments arranged by size: 

                1792     483     269
 
PvuII CAG'CTG

Cuts at:      0     423     564    1751    2544 
   Size:        423     141    1187     793

  Fragments arranged by size: 

                1187     793     423     141
 
SacII CC_GC'GG

Cuts at:      0     113     187    2544 
   Size:        113      74    2357

  Fragments arranged by size: 

                2357     113      74
 
SapI GCTCTTCn'nnn_

Cuts at:      0     737    1250    2544 
   Size:        737     513    1294

  Fragments arranged by size: 

                1294     737     513
 
ScaI AGT'ACT

Cuts at:      0     388    1661    2544 
   Size:        388    1273     883

  Fragments arranged by size: 

                1273     883     388
 
SexAI A'CCwGG_T

Cuts at:      0     757    2544 
   Size:        757    1787
 
SfcI C'TryA_G

Cuts at:      0     265     748    2544 
   Size:        265     483    1796

  Fragments arranged by size: 

                1796     483     265
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     127    2544 
   Size:        127    2417
 
SmlI C'TyrA_G

Cuts at:      0     517    1127    2544 
   Size:        517     610    1417

  Fragments arranged by size: 

                1417     610     517
 
SpeI A'CTAG_T

Cuts at:      0     888    2544 
   Size:        888    1656
 
SphI G_CATG'C

Cuts at:      0      36    2544 
   Size:         36    2508
 
StuI AGG'CCT

Cuts at:      0     616    2544 
   Size:        616    1928
 
StyI C'CwwG_G

Cuts at:      0     125     806    1607    2544 
   Size:        125     681     801     937

  Fragments arranged by size: 

                 937     801     681     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     571    2128    2544 
   Size:        571    1557     416

  Fragments arranged by size: 

                1557     571     416
 
TatI w'GTAC_w

Cuts at:      0     386    1659    2544 
   Size:        386    1273     885

  Fragments arranged by size: 

                1273     885     386
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424    2544 
   Size:        392      32    2120

  Fragments arranged by size: 

                2120     392      32
 
XhoI C'TCGA_G

Cuts at:      0    1127    2544 
   Size:       1127    1417
 
XmnI GAAnn'nnTTC

Cuts at:      0    1956    2468    2544 
   Size:       1956     512      76

  Fragments arranged by size: 

                1956     512      76
 
ZraI GAC'GTC

Cuts at:      0      90    2544 
   Size:         90    2454
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AlfI    AlwNI     ApaI     ApoI
     AseI     AvaI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I  HindIII     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PfoI     PsiI   PspOMI    PspXI
     PstI    PvuII    SacII     SapI     ScaI    SexAI     SfcI     SfiI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BglII     BmtI     BplI     BsaI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BsrFI    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XmaI
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