Restriction map for pF1KA0985
 (Linear) (Six-base) MAPSORT of: KIAA0985.seq  Check: 7927  from: 1  to: 2082



KIAA0985.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1611    2082 
   Size:       1611     471
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1276    1531    2082 
   Size:       1276     255     551

  Fragments arranged by size: 

                1276     551     255
 
AfeI AGC'GCT

Cuts at:      0    1990    2041    2082 
   Size:       1990      51      41

  Fragments arranged by size: 

                1990      51      41
 
AflIII A'CryG_T

Cuts at:      0    2069    2082 
   Size:       2069      13
 
AleI CACnn'nnGTG

Cuts at:      0     110    2082 
   Size:        110    1972
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1748    2082 
   Size:       1748     334
 
AlwNI CAG_nnn'CTG

Cuts at:      0     652     664    2082 
   Size:        652      12    1418

  Fragments arranged by size: 

                1418     652      12
 
ApaI G_GGCC'C

Cuts at:      0     634    2082 
   Size:        634    1448
 
ApaLI G'TGCA_C

Cuts at:      0    1230    1723    2082 
   Size:       1230     493     359

  Fragments arranged by size: 

                1230     493     359
 
ApoI r'AATT_y

Cuts at:      0    1189    1459    1474    1530    1846    2082 
   Size:       1189     270      15      56     316     236

  Fragments arranged by size: 

                1189     316     270     236      56      15
 
AvaI C'yCGr_G

Cuts at:      0     188     593     605     633     670     983    1592    2082 
   Size:        188     405      12      28      37     313     609     490

  Fragments arranged by size: 

                 609     490     405     313     188      37      28      12
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1995    2028    2082 
   Size:       1995      33      54

  Fragments arranged by size: 

                1995      54      33
 
BanI G'GyrC_C

Cuts at:      0    1180    2045    2082 
   Size:       1180     865      37

  Fragments arranged by size: 

                1180     865      37
 
BanII G_rGCy'C

Cuts at:      0     328     581     600     634     727     790     856    2082 
   Size:        328     253      19      34      93      63      66    1226

  Fragments arranged by size: 

                1226     328     253      93      66      63      34      19
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     526     649    1432    2082 
   Size:        526     123     783     650

  Fragments arranged by size: 

                 783     650     526     123
 
BbvCI CC'TCA_GC

Cuts at:      0     866    2082 
   Size:        866    1216
 
BglI GCCn_nnn'nGGC

Cuts at:      0     126     712    2082 
   Size:        126     586    1370

  Fragments arranged by size: 

                1370     586     126
 
Bme1580I G_kGCm'C

Cuts at:      0     634    1185    1234    1727    2082 
   Size:        634     551      49     493     355

  Fragments arranged by size: 

                 634     551     493     355      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41    2082 
   Size:         41    2041
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     293     479     584     762     791     810    1567 
   Size:         70     223     186     105     178      29      19     757

Cuts at:   1567    2082 
   Size:        515

  Fragments arranged by size: 

                 757     515     223     186     178     105      70      29
                  19
 
Bpu10I CC'TnA_GC

Cuts at:      0     575     866    2082 
   Size:        575     291    1216

  Fragments arranged by size: 

                1216     575     291
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1486    2082 
   Size:       1486     596
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     379    1375    1480    1671    2082 
   Size:        379     996     105     191     411

  Fragments arranged by size: 

                 996     411     379     191     105
 
BsaAI yAC'GTr

Cuts at:      0    2070    2082 
   Size:       2070      12
 
BsaHI Gr'CG_yC

Cuts at:      0     231    2082 
   Size:        231    1851
 
BsaWI w'CCGG_w

Cuts at:      0    1057    1785    2082 
   Size:       1057     728     297

  Fragments arranged by size: 

                1057     728     297
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     266     296     479     509    1204    1234    2082 
   Size:        266      30     183      30     695      30     848

  Fragments arranged by size: 

                 848     695     266     183      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172    1003    1141    1144    1660    1870    2082 
   Size:        172     831     138       3     516     210     212

  Fragments arranged by size: 

                 831     516     212     210     172     138       3
 
BseYI C'CCAG_C

Cuts at:      0     312     321     530     657     749     789     855    1017 
   Size:        312       9     209     127      92      40      66     162

Cuts at:   1017    1091    1573    1965    2082 
   Size:         74     482     392     117

  Fragments arranged by size: 

                 482     392     312     209     162     127     117      92
                  74      66      40       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     868    1285    2082 
   Size:        351     517     417     797

  Fragments arranged by size: 

                 797     517     417     351
 
BsiHKAI G_wGCw'C

Cuts at:      0     727    1234    1727    2082 
   Size:        727     507     493     355

  Fragments arranged by size: 

                 727     507     493     355
 
BsmI GAATG_Cn'

Cuts at:      0     402    2082 
   Size:        402    1680
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     795    2082 
   Size:        795    1287
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     581     600     634     727     790     856    1185 
   Size:        328     253      19      34      93      63      66     329

Cuts at:   1185    1234    1727    2082 
   Size:         49     493     355

  Fragments arranged by size: 

                 493     355     329     328     253      93      66      63
                  49      34      19
 
BspEI T'CCGG_A

Cuts at:      0    1057    2082 
   Size:       1057    1025
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     784     840    1611    2082 
   Size:        784      56     771     471

  Fragments arranged by size: 

                 784     771     471      56
 
BsrFI r'CCGG_y

Cuts at:      0      53    2082 
   Size:         53    2029
 
BsrGI T'GTAC_A

Cuts at:      0    1676    2082 
   Size:       1676     406
 
BssSI C'ACGA_G

Cuts at:      0    1387    2082 
   Size:       1387     695
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     374     425    1308    2082 
   Size:        374      51     883     774

  Fragments arranged by size: 

                 883     774     374      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     112    2082 
   Size:        112    1970
 
BstYI r'GATC_y

Cuts at:      0    1436    1658    1970    2082 
   Size:       1436     222     312     112

  Fragments arranged by size: 

                1436     312     222     112
 
Bsu36I CC'TnA_GG

Cuts at:      0    1432    2082 
   Size:       1432     650
 
BtgI C'CryG_G

Cuts at:      0    1262    1736    2082 
   Size:       1262     474     346

  Fragments arranged by size: 

                1262     474     346
 
BtsI GCAGTG_nn'

Cuts at:      0     971    1234    2082 
   Size:        971     263     848

  Fragments arranged by size: 

                 971     848     263
 
DraIII CAC_nnn'GTG

Cuts at:      0     374    2082 
   Size:        374    1708
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1584    1765    1912    2082 
   Size:       1584     181     147     170

  Fragments arranged by size: 

                1584     181     170     147
 
EaeI y'GGCC_r

Cuts at:      0    1464    2082 
   Size:       1464     618
 
EarI CTCTTCn'nnn_

Cuts at:      0     137     200    1130    1610    2082 
   Size:        137      63     930     480     472

  Fragments arranged by size: 

                 930     480     472     137      63
 
EcoICRI GAG'CTC

Cuts at:      0     725    2082 
   Size:        725    1357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     293     479     584     762     791     810    1276 
   Size:         70     223     186     105     178      29      19     466

Cuts at:   1276    1531    1567    2082 
   Size:        255      36     515

  Fragments arranged by size: 

                 515     466     255     223     186     178     105      70
                  36      29      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     836    1609    2082 
   Size:        836     773     473

  Fragments arranged by size: 

                 836     773     473
 
EcoO109I rG'GnC_Cy

Cuts at:      0     492     630     649     704     884     907    1251    2082 
   Size:        492     138      19      55     180      23     344     831

  Fragments arranged by size: 

                 831     492     344     180     138      55      23      19
            
 
EcoRI G'AATT_C

Cuts at:      0    1189    1846    2082 
   Size:       1189     657     236

  Fragments arranged by size: 

                1189     657     236
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1851    2082 
   Size:       1851     231
 
FspI TGC'GCA

Cuts at:      0     700    2082 
   Size:        700    1382
 
HaeII r_GCGC'y

Cuts at:      0     862    1992    2043    2082 
   Size:        862    1130      51      39

  Fragments arranged by size: 

                1130     862      51      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     646     678    1204    1236    1484    1516    2082 
   Size:        646      32     526      32     248      32     566

  Fragments arranged by size: 

                 646     566     526     248      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1333    2082 
   Size:       1333     749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1005    1352    2082 
   Size:         16     989     347     730

  Fragments arranged by size: 

                 989     730     347      16
 
MscI TGG'CCA

Cuts at:      0    1466    2082 
   Size:       1466     616
 
MslI CAynn'nnrTG

Cuts at:      0     110    2082 
   Size:        110    1972
 
MspA1I CmG'CkG

Cuts at:      0     290     312    1084    1965    2082 
   Size:        290      22     772     881     117

  Fragments arranged by size: 

                 881     772     290     117      22
 
NcoI C'CATG_G

Cuts at:      0    1262    1736    2082 
   Size:       1262     474     346

  Fragments arranged by size: 

                1262     474     346
 
NspI r_CATG'y

Cuts at:      0    1419    2082 
   Size:       1419     663
 
PasI CC'CwG_GG

Cuts at:      0     964    1176    2082 
   Size:        964     212     906

  Fragments arranged by size: 

                 964     906     212
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1024    2082 
   Size:       1024    1058
 
PmlI CAC'GTG

Cuts at:      0    2070    2082 
   Size:       2070      12
 
PpuMI rG'GwC_Cy

Cuts at:      0     492     884     907    2082 
   Size:        492     392      23    1175

  Fragments arranged by size: 

                1175     492     392      23
 
PspOMI G'GGCC_C

Cuts at:      0     630    2082 
   Size:        630    1452
 
PspXI vC'TCGA_Gb

Cuts at:      0     188     605    2082 
   Size:        188     417    1477

  Fragments arranged by size: 

                1477     417     188
 
PstI C_TGCA'G

Cuts at:      0    1083    1104    1229    2082 
   Size:       1083      21     125     853

  Fragments arranged by size: 

                1083     853     125      21
 
PvuII CAG'CTG

Cuts at:      0     312    1084    1965    2082 
   Size:        312     772     881     117

  Fragments arranged by size: 

                 881     772     312     117
 
SacI G_AGCT'C

Cuts at:      0     727    2082 
   Size:        727    1355
 
SanDI GG'GwC_CC

Cuts at:      0     884    2082 
   Size:        884    1198
 
SapI GCTCTTCn'nnn_

Cuts at:      0     137    1610    2082 
   Size:        137    1473     472

  Fragments arranged by size: 

                1473     472     137
 
SfcI C'TryA_G

Cuts at:      0    1079    1100    1225    2053    2082 
   Size:       1079      21     125     828      29

  Fragments arranged by size: 

                1079     828     125      29      21
 
SmaI CCC'GGG

Cuts at:      0     595     635     672    2082 
   Size:        595      40      37    1410

  Fragments arranged by size: 

                1410     595      40      37
 
SmlI C'TyrA_G

Cuts at:      0     188     605    1501    2082 
   Size:        188     417     896     581

  Fragments arranged by size: 

                 896     581     417     188
 
SrfI GCCC'GGGC

Cuts at:      0     595    2082 
   Size:        595    1487
 
StuI AGG'CCT

Cuts at:      0     823    1697    2082 
   Size:        823     874     385

  Fragments arranged by size: 

                 874     823     385
 
StyI C'CwwG_G

Cuts at:      0    1247    1262    1736    1979    2082 
   Size:       1247      15     474     243     103

  Fragments arranged by size: 

                1247     474     243     103      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1055    1168    2082 
   Size:       1055     113     914

  Fragments arranged by size: 

                1055     914     113
 
TatI w'GTAC_w

Cuts at:      0    1676    2082 
   Size:       1676     406
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     266     298    2082 
   Size:        221      32      13      32    1784

  Fragments arranged by size: 

                1784     221      32      32      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     905    2082 
   Size:        905    1177
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     378    2082 
   Size:        378    1704
 
XhoI C'TCGA_G

Cuts at:      0     188     605    2082 
   Size:        188     417    1477

  Fragments arranged by size: 

                1477     417     188
 
XmaI C'CCGG_G

Cuts at:      0     593     633     670    2082 
   Size:        593      40      37    1412

  Fragments arranged by size: 

                1412     593      40      37
 
XmnI GAAnn'nnTTC

Cuts at:      0    1531    2082 
   Size:       1531     551
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrFI    BsrGI    BssSI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI    PflMI     PmlI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SanDI     SapI     SfcI     SmaI     SmlI     SrfI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BsaBI
    BsiEI    BsiWI    BspHI    BsrBI    BsrDI   BssHII    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI     EciI    EcoRV
     FseI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI
     XbaI     ZraI
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