Restriction map for pF1KA0930
 (Linear) (Six-base) MAPSORT of: KIAA0930.seq  Check: 9362  from: 1  to: 1239



KIAA0930.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0     811    1239 
   Size:        811     428
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     860     873    1138    1239 
   Size:        860      13     265     101

  Fragments arranged by size: 

                 860     265     101      13
 
AflIII A'CryG_T

Cuts at:      0     394     614    1239 
   Size:        394     220     625

  Fragments arranged by size: 

                 625     394     220
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1239 
   Size:        363     876
 
ApaI G_GGCC'C

Cuts at:      0     769    1239 
   Size:        769     470
 
ApaLI G'TGCA_C

Cuts at:      0     390    1239 
   Size:        390     849
 
BanI G'GyrC_C

Cuts at:      0     957    1239 
   Size:        957     282
 
BanII G_rGCy'C

Cuts at:      0     162     643     769    1239 
   Size:        162     481     126     470

  Fragments arranged by size: 

                 481     470     162     126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     108    1239 
   Size:        108    1131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     208     242    1239 
   Size:        208      34     997

  Fragments arranged by size: 

                 997     208      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      51     703     773     793    1239 
   Size:         51     652      70      20     446

  Fragments arranged by size: 

                 652     446      70      51      20
 
BlpI GC'TnA_GC

Cuts at:      0     248    1239 
   Size:        248     991
 
Bme1580I G_kGCm'C

Cuts at:      0     394     769     962    1090    1239 
   Size:        394     375     193     128     149

  Fragments arranged by size: 

                 394     375     193     149     128
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     333     452    1239 
   Size:        333     119     787

  Fragments arranged by size: 

                 787     333     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     834    1239 
   Size:        834     405
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     931    1239 
   Size:        646     285     308

  Fragments arranged by size: 

                 646     308     285
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     302    1076    1239 
   Size:        302     774     163

  Fragments arranged by size: 

                 774     302     163
 
BsaAI yAC'GTr

Cuts at:      0     397    1239 
   Size:        397     842
 
BsaWI w'CCGG_w

Cuts at:      0     968    1239 
   Size:        968     271
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     563     593    1239 
   Size:        563      30     646

  Fragments arranged by size: 

                 646     563      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     502     553    1027    1239 
   Size:        343     159      51     474     212

  Fragments arranged by size: 

                 474     343     212     159      51
 
BseYI C'CCAG_C

Cuts at:      0     855    1239 
   Size:        855     384
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1033    1239 
   Size:        248     785     206

  Fragments arranged by size: 

                 785     248     206
 
BsiHKAI G_wGCw'C

Cuts at:      0     394    1239 
   Size:        394     845
 
Bsp1286I G_dGCh'C

Cuts at:      0     162     394     643     769     962    1090    1239 
   Size:        162     232     249     126     193     128     149

  Fragments arranged by size: 

                 249     232     193     162     149     128     126
 
BspHI T'CATG_A

Cuts at:      0     521    1239 
   Size:        521     718
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1073    1239 
   Size:       1073     166
 
BsrBI CCG'CTC

Cuts at:      0     876    1239 
   Size:        876     363
 
BsrFI r'CCGG_y

Cuts at:      0     275     920    1239 
   Size:        275     645     319

  Fragments arranged by size: 

                 645     319     275
 
BssSI C'ACGA_G

Cuts at:      0     871    1239 
   Size:        871     368
 
BstEII G'GTnAC_C

Cuts at:      0     375     879    1239 
   Size:        375     504     360

  Fragments arranged by size: 

                 504     375     360
 
Bsu36I CC'TnA_GG

Cuts at:      0     254    1239 
   Size:        254     985
 
BtgI C'CryG_G

Cuts at:      0     470    1239 
   Size:        470     769
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112    1239 
   Size:        112    1127
 
EaeI y'GGCC_r

Cuts at:      0      23      52     692     998    1239 
   Size:         23      29     640     306     241

  Fragments arranged by size: 

                 640     306     241      29      23
 
EarI CTCTTCn'nnn_

Cuts at:      0     836    1239 
   Size:        836     403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     834     860     873    1138    1239 
   Size:        834      26      13     265     101

  Fragments arranged by size: 

                 834     265     101      26      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765     766    1109    1239 
   Size:        765       1     343     130

  Fragments arranged by size: 

                 765     343     130       1
 
FspI TGC'GCA

Cuts at:      0     757    1239 
   Size:        757     482
 
FspAI rTGC'GCAy

Cuts at:      0     757    1239 
   Size:        757     482
 
HaeII r_GCGC'y

Cuts at:      0     661    1239 
   Size:        661     578
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     563     595    1239 
   Size:        563      32     644

  Fragments arranged by size: 

                 644     563      32
 
MscI TGG'CCA

Cuts at:      0      25    1239 
   Size:         25    1214
 
MslI CAynn'nnrTG

Cuts at:      0    1231    1239 
   Size:       1231       8
 
MspA1I CmG'CkG

Cuts at:      0     247    1182    1239 
   Size:        247     935      57

  Fragments arranged by size: 

                 935     247      57
 
NcoI C'CATG_G

Cuts at:      0     470    1239 
   Size:        470     769
 
NspI r_CATG'y

Cuts at:      0     180    1239 
   Size:        180    1059
 
PasI CC'CwG_GG

Cuts at:      0     771    1239 
   Size:        771     468
 
PfoI T'CCnGG_A

Cuts at:      0    1022    1205    1239 
   Size:       1022     183      34

  Fragments arranged by size: 

                1022     183      34
 
PmlI CAC'GTG

Cuts at:      0     397    1239 
   Size:        397     842
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     529     561    1239 
   Size:        529      32     678

  Fragments arranged by size: 

                 678     529      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1109    1239 
   Size:       1109     130
 
PspOMI G'GGCC_C

Cuts at:      0     765    1239 
   Size:        765     474
 
PstI C_TGCA'G

Cuts at:      0      90     359    1239 
   Size:         90     269     880

  Fragments arranged by size: 

                 880     269      90
 
PvuII CAG'CTG

Cuts at:      0     247    1239 
   Size:        247     992
 
SfcI C'TryA_G

Cuts at:      0      86     355     812    1239 
   Size:         86     269     457     427

  Fragments arranged by size: 

                 457     427     269      86
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      51    1239 
   Size:         51    1188
 
SmlI C'TyrA_G

Cuts at:      0     661     946    1239 
   Size:        661     285     293

  Fragments arranged by size: 

                 661     293     285
 
StuI AGG'CCT

Cuts at:      0     302    1239 
   Size:        302     937
 
StyI C'CwwG_G

Cuts at:      0      26     470    1239 
   Size:         26     444     769

  Fragments arranged by size: 

                 769     444      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1064    1239 
   Size:       1064     175
 
TatI w'GTAC_w

Cuts at:      0     734    1239 
   Size:        734     505
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144    1239 
   Size:        144    1095
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI    ApaLI     BanI    BanII
     BbsI     BcgI     BglI     BlpI Bme1580I     BmrI     BpmI    BpuEI
     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstEII   Bsu36I
     BtgI     DrdI     EaeI     EarI  Eco57MI EcoO109I     FspI    FspAI
    HaeII    Hin4I     MscI     MslI   MspA1I     NcoI     NspI     PasI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SfcI
     SfiI     SmlI     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsaHI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com