Restriction map for pF1KA0925
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AflII       AgeI        AhdI        AlwNI       AocI        
ApaI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        BbeI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlpI        Bpu1102I    BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccI           43  GTCTAC               GT^[AC][GT]AC
AccIII         35  TCCGGA               T^CCGGA
AcyI            8  GGCGTC               G[GA]^CG[CT]C
AfaI          873  GTAC                 GT^AC
AflIII        311  ACACGT               A^C[GA][CT]GT
Alw44I        103  GTGCAC               G^TGCAC
Aor51HI       448  AGCGCT               AGC^GCT
ApaLI         103  GTGCAC               G^TGCAC
Asp700I       270  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          337  CCCGAG               C^[CT]CG[GA]G
AviII        1403  TGCGCA               TGC^GCA
AvrII         948  CCTAGG               C^CTAGG
BamHI          83  GGATCC               G^GATCC
BanI          419  GGCACC               G^G[CT][GA]CC
BbrPI         576  CACGTG               CAC^GTG
BbsI         1081  GAAGAC               GAAGAC(2/6)
BfaI          949  CTAG                 C^TAG
BglI          239  GCCACCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          948  CCTAGG               C^CTAGG
BpuAI        1081  GAAGAC               GAAGAC(2/6)
BsaAI         576  CACGTG               [CT]AC^GT[GA]
BsaBI          84  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           8  GGCGTC               G[GA]^CG[CT]C
BsaI          708  GGTCTC               GGTCTC(1/5)
BseAI          35  TCCGGA               T^CCGGA
BsgI          382  CTGCAC               GTGCAG(16/14)
BsmAI         709  GTCTC                GTCTC(1/5)
BsmI         1370  GCATTC               GAATGC(1/-1)
BsoBI         337  CCCGAG               C^[CT]CG[GA]G
BspEI          35  TCCGGA               T^CCGGA
BssSI         297  CTCGTG               CACGAG(-5/-1)
BstBI        1301  TTCGAA               TT^CGAA
DrdI          460  GACTACAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      448  AGCGCT               AGC^GCT
EcoRV         259  GATATC               GAT^ATC
FspI         1403  TGCGCA               TGC^GCA
HgaI            9  GCGTC                GACGC(5/10)
Hin1I           8  GGCGTC               G[GA]^CG[CT]C
MaeI          949  CTAG                 C^TAG
MamI           84  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           35  TCCGGA               T^CCGGA
MseI         1211  TTAA                 T^TAA
MspA1I       1384  CAGCTG               C[AC]G^C[GT]G
NaeI          395  GCCGGC               GCC^GGC
NgoMIV        395  GCCGGC               G^CCGGC
NspV         1301  TTCGAA               TT^CGAA
PleI         1017  GACTC                GAGTC(4/5)
PmaCI         576  CACGTG               CAC^GTG
PmlI          576  CACGTG               CAC^GTG
PshAI         702  GACAGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          628  CTGCAG               CTGCA^G
PvuII        1384  CAGCTG               CAG^CTG
RsaI          873  GTAC                 GT^AC
SacI         1294  GAGCTC               GAGCT^C
SfcI          628  CTGCAG               C^T[GA][CT]AG
SfuI         1301  TTCGAA               TT^CGAA
SmlI         1021  CTCAAG               C^T[CT][GA]AG
StuI          945  AGGCCT               AGG^CCT
Tru9I        1211  TTAA                 T^TAA
Tsp509I       783  AATT                 ^AATT
XcmI          962  CCAAGCACGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          270  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BalI           56  TGGCCA               TGG^CCA
              143  TGGCCA              
BsaWI          35  TCCGGA               [AT]^CCGG[AT]
              869  TCCGGT              
BseRI         694  CTCCTC               GAGGAG(10/8)
             1345  GAGGAG              
BsmFI         743  GGGAC                GGGAC(10/14)
             1273  GTCCC               
BsrBI         612  CCGCTC               CCGCTC(-3/-3)
              924  CCGCTC              
BsrDI         291  CATTGC               GCAATG(2/0)
              248  GCAATG              
BsrFI         395  GCCGGC               [GA]^CCGG[CT]
              498  GCCGGT              
Cfr10I        395  GCCGGC               [GA]^CCGG[CT]
              498  GCCGGT              
DdeI          890  CTGAG                C^T[ACGT]AG
              904  CTGAG               
DraIII        573  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              652  CACAGAGTG           
EarI          775  GAAGAG               CTCTTC(1/4)
             1233  GAAGAG              
EcoT14I       227  CCAAGG               C^C[AT][AT]GG
              948  CCTAGG              
HaeII         369  GGCGCT               [GA]GCGC^[CT]
              448  AGCGCT              
HincII        127  GTCAAC               GT[CT]^[GA]AC
             1246  GTCAAC              
HindII        127  GTCAAC               GT[CT]^[GA]AC
             1246  GTCAAC              
Ksp632I       775  GAAGAG               CTCTTC(1/4)
             1233  GAAGAG              
MaeII         313  ACGT                 A^CGT
              577  ACGT                
MluNI          56  TGGCCA               TGG^CCA
              143  TGGCCA              
MscI           56  TGGCCA               TGG^CCA
              143  TGGCCA              
NspI          539  GCATGT               [GA]CATG^[CT]
              833  ACATGC              
PpuMI         467  AGGTCCT              [GA]G^G[AT]CC[CT]
              752  AGGACCT             
SfaNI         820  GATGC                GCATC(5/9)
             1145  GCATC               
StyI          227  CCAAGG               C^C[AT][AT]GG
              948  CCTAGG              
TfiI         1069  GATTC                G^A[AT]TC
             1329  GATTC               
Tsp45I        151  GTCAC                ^GT[GC]AC
             1015  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TaqI        
TseI        TspRI       TthHB8I     XhoII       
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