(Circular) (Six-base) MAPSORT of: pF1KA0919.seq Check: 5935 from: 1 to: 5129 pF1KA0919 5129 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AccI GT'mk_AC Cuts at: 468 1002 2036 2167 468 Size: 534 1034 131 3430 Fragments arranged by size: 3430 1034 534 131 Acc65I G'GTAC_C Cuts at: 954 2145 954 Size: 1191 3938 AclI AA'CG_TT Cuts at: 4969 4969 Size: 5129 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 478 1327 1654 1688 1946 2954 3386 3739 4401 Size: 849 327 34 258 1008 432 353 662 Cuts at: 4401 478 Size: 1206 Fragments arranged by size: 1206 1008 849 662 432 353 327 258 34 AflIII A'CryG_T Cuts at: 2481 4281 4562 2481 Size: 1800 281 3048 Fragments arranged by size: 3048 1800 281 AleI CACnn'nnGTG Cuts at: 1852 1852 Size: 5129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 596 1747 596 Size: 1151 3978 AlwNI CAG_nnn'CTG Cuts at: 2434 3872 2434 Size: 1438 3691 ApaI G_GGCC'C Cuts at: 212 966 212 Size: 754 4375 ApaLI G'TGCA_C Cuts at: 3967 3967 Size: 5129 ApoI r'AATT_y Cuts at: 917 1582 1615 1831 2133 4482 4738 917 Size: 665 33 216 302 2349 256 1308 Fragments arranged by size: 2349 1308 665 302 256 216 33 AseI AT'TA_AT Cuts at: 20 508 20 Size: 488 4641 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5129 AvaI C'yCGr_G Cuts at: 1028 2149 1028 Size: 1121 4008 BamHI G'GATC_C Cuts at: 167 2154 167 Size: 1987 3142 BanI G'GyrC_C Cuts at: 954 1024 1891 2145 2559 2807 2842 954 Size: 70 867 254 414 248 35 3241 Fragments arranged by size: 3241 867 414 254 248 70 35 BanII G_rGCy'C Cuts at: 212 966 2143 3173 212 Size: 754 1177 1030 2168 Fragments arranged by size: 2168 1177 1030 754 BbeI G_GCGC'C Cuts at: 2563 2811 2563 Size: 248 4881 BbsI GAAGACnn'nnnn_ Cuts at: 511 938 511 Size: 427 4702 BciVI GTATCCnnnnn_n' Cuts at: 286 393 3027 4083 5129 286 Size: 107 2634 1056 1046 286 Fragments arranged by size: 2634 1056 1046 286 107 BclI T'GATC_A Cuts at: 2649 2649 Size: 5129 BfrBI ATG'CAT Cuts at: 856 1107 856 Size: 251 4878 BglII A'GATC_T Cuts at: 1647 2644 4681 1647 Size: 997 2037 2095 Fragments arranged by size: 2095 2037 997 BlpI GC'TnA_GC Cuts at: 2241 2241 Size: 5129 Bme1580I G_kGCm'C Cuts at: 212 966 1029 2754 2847 3971 212 Size: 754 63 1725 93 1124 1370 Fragments arranged by size: 1725 1370 1124 754 93 63 BmrI ACTGGGnnnn_n' Cuts at: 2341 2422 2757 4901 2341 Size: 81 335 2144 2569 Fragments arranged by size: 2569 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1745 3496 1745 Size: 1751 3378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 163 745 971 163 Size: 582 226 4321 Fragments arranged by size: 4321 582 226 Bpu10I CC'TnA_GC Cuts at: 217 217 Size: 5129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 608 2305 3184 3898 4196 608 Size: 1697 879 714 298 1541 Fragments arranged by size: 1697 1541 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1391 4833 1391 Size: 3442 1687 BsaAI yAC'GTr Cuts at: 2482 2482 Size: 5129 BsaBI GATnn'nnATC Cuts at: 6 1652 1786 2643 4680 6 Size: 1646 134 857 2037 455 Fragments arranged by size: 2037 1646 857 455 134 BsaHI Gr'CG_yC Cuts at: 2560 2808 3510 5006 2560 Size: 248 702 1496 2683 Fragments arranged by size: 2683 1496 702 248 BsaWI w'CCGG_w Cuts at: 1999 2318 2839 3517 3928 4075 4349 1999 Size: 319 521 678 411 147 274 2779 Fragments arranged by size: 2779 678 521 411 319 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 380 380 Size: 5129 BseYI C'CCAG_C Cuts at: 779 2554 3312 3977 4385 779 Size: 1775 758 665 408 1523 Fragments arranged by size: 1775 1523 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 566 566 Size: 5129 BsiEI CG_ry'CG Cuts at: 104 291 2671 2717 3947 104 Size: 187 2380 46 1230 1286 Fragments arranged by size: 2380 1286 1230 187 46 BsiHKAI G_wGCw'C Cuts at: 161 190 2143 2921 3111 3971 161 Size: 29 1953 778 190 860 1319 Fragments arranged by size: 1953 1319 860 778 190 29 Bsp1286I G_dGCh'C Cuts at: 161 190 212 966 1029 2143 2754 2847 2921 Size: 29 22 754 63 1114 611 93 74 Cuts at: 2921 3111 3173 3971 161 Size: 190 62 798 1319 Fragments arranged by size: 1319 1114 798 754 611 190 93 74 63 62 29 22 BspHI T'CATG_A Cuts at: 3561 3561 Size: 5129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 626 1423 2179 2393 2695 3076 626 Size: 797 756 214 302 381 2679 Fragments arranged by size: 2679 797 756 381 302 214 BsrBI CCG'CTC Cuts at: 50 114 165 3421 3475 4959 5124 50 Size: 64 51 3256 54 1484 165 55 Fragments arranged by size: 3256 1484 165 64 55 54 51 BsrDI GCAATG_nn' Cuts at: 452 2042 3041 452 Size: 1590 999 2540 Fragments arranged by size: 2540 1590 999 BsrFI r'CCGG_y Cuts at: 3127 3308 3127 Size: 181 4948 BsrGI T'GTAC_A Cuts at: 536 1499 536 Size: 963 4166 BssSI C'ACGA_G Cuts at: 160 3400 4108 160 Size: 3240 708 1181 Fragments arranged by size: 3240 1181 708 BstAPI GCAn_nnn'nTGC Cuts at: 2506 2506 Size: 5129 BstBI TT'CG_AA Cuts at: 3490 3490 Size: 5129 BstYI r'GATC_y Cuts at: 1 167 1647 2154 2621 2644 2979 3225 3531 Size: 166 1480 507 467 23 335 246 306 Cuts at: 3531 3543 3629 4681 1 Size: 12 86 1052 449 Fragments arranged by size: 1480 1052 507 467 449 335 306 246 166 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 5129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1236 3079 3260 3423 1236 Size: 1843 181 163 2942 Fragments arranged by size: 2942 1843 181 163 BtsI GCAGTG_nn' Cuts at: 469 2372 469 Size: 1903 3226 ClaI AT'CG_AT Cuts at: 639 996 639 Size: 357 4772 DraI TTT'AAA Cuts at: 2128 2128 Size: 5129 DrdI GACnn_nn'nnGTC Cuts at: 2835 4179 2835 Size: 1344 3785 EaeI y'GGCC_r Cuts at: 2714 2888 3279 3306 4651 2714 Size: 174 391 27 1345 3192 Fragments arranged by size: 3192 1345 391 174 27 EagI C'GGCC_G Cuts at: 2714 2714 Size: 5129 EarI CTCTTCn'nnn_ Cuts at: 446 944 1487 1925 3152 3362 446 Size: 498 543 438 1227 210 2213 Fragments arranged by size: 2213 1227 543 498 438 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 142 148 3337 4079 4225 4939 142 Size: 6 3189 742 146 714 332 Fragments arranged by size: 3189 742 714 332 146 6 EcoICRI GAG'CTC Cuts at: 2141 2141 Size: 5129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 163 478 745 971 1327 1654 1688 1946 2954 Size: 315 267 226 356 327 34 258 1008 Cuts at: 2954 3386 3739 4401 163 Size: 432 353 662 891 Fragments arranged by size: 1008 891 662 432 356 353 327 315 267 258 226 34 EcoO109I rG'GnC_Cy Cuts at: 962 2268 962 Size: 1306 3823 EcoRI G'AATT_C Cuts at: 917 2133 917 Size: 1216 3913 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 5129 FspI TGC'GCA Cuts at: 2910 2910 Size: 5129 HaeII r_GCGC'y Cuts at: 2563 2811 4041 4812 2563 Size: 248 1230 771 2880 Fragments arranged by size: 2880 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2971 3001 3003 3033 2971 Size: 30 2 30 5067 Fragments arranged by size: 5067 30 30 2 HincII GTy'rAC Cuts at: 469 2168 469 Size: 1699 3430 HindIII A'AGCT_T Cuts at: 262 673 262 Size: 411 4718 KasI G'GCGC_C Cuts at: 2559 2807 2559 Size: 248 4881 KpnI G_GTAC'C Cuts at: 958 2149 958 Size: 1191 3938 MluI A'CGCG_T Cuts at: 4562 4562 Size: 5129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3888 4072 3888 Size: 184 4945 MscI TGG'CCA Cuts at: 2890 2890 Size: 5129 MslI CAynn'nnrTG Cuts at: 1852 3118 3245 1852 Size: 1266 127 3736 Fragments arranged by size: 3736 1266 127 MspA1I CmG'CkG Cuts at: 151 1924 1977 2240 2554 2914 3698 3943 151 Size: 1773 53 263 314 360 784 245 1337 Fragments arranged by size: 1773 1337 784 360 314 263 245 53 NaeI GCC'GGC Cuts at: 3310 3310 Size: 5129 NarI GG'CG_CC Cuts at: 2560 2808 2560 Size: 248 4881 NcoI C'CATG_G Cuts at: 107 107 Size: 5129 NgoMIV G'CCGG_C Cuts at: 3308 3308 Size: 5129 NsiI A_TGCA'T Cuts at: 858 1109 858 Size: 251 4878 NspI r_CATG'y Cuts at: 1107 1383 1921 2182 2682 4285 1107 Size: 276 538 261 500 1603 1951 Fragments arranged by size: 1951 1603 538 500 276 261 PasI CC'CwG_GG Cuts at: 1191 1191 Size: 5129 PciI A'CATG_T Cuts at: 4281 4281 Size: 5129 PflMI CCAn_nnn'nTGG Cuts at: 972 972 Size: 5129 PmeI GTTT'AAAC Cuts at: 2128 2128 Size: 5129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 3575 Size: 32 5097 PspOMI G'GGCC_C Cuts at: 208 962 208 Size: 754 4375 PstI C_TGCA'G Cuts at: 1736 2176 2861 1736 Size: 440 685 4004 Fragments arranged by size: 4004 685 440 PvuI CG_AT'CG Cuts at: 104 104 Size: 5129 PvuII CAG'CTG Cuts at: 1924 1977 2554 2914 1924 Size: 53 577 360 4139 Fragments arranged by size: 4139 577 360 53 RsrII CG'GwC_CG Cuts at: 3324 3324 Size: 5129 SacI G_AGCT'C Cuts at: 2143 2143 Size: 5129 SalI G'TCGA_C Cuts at: 467 2166 467 Size: 1699 3430 SapI GCTCTTCn'nnn_ Cuts at: 446 3152 3362 446 Size: 2706 210 2213 Fragments arranged by size: 2706 2213 210 SbfI CC_TGCA'GG Cuts at: 2176 2176 Size: 5129 ScaI AGT'ACT Cuts at: 888 1822 4646 888 Size: 934 2824 1371 Fragments arranged by size: 2824 1371 934 SexAI A'CCwGG_T Cuts at: 2030 2030 Size: 5129 SfcI C'TryA_G Cuts at: 33 1125 1467 1732 1972 2172 2857 3825 4016 Size: 1092 342 265 240 200 685 968 191 Cuts at: 4016 33 Size: 1146 Fragments arranged by size: 1146 1092 968 685 342 265 240 200 191 SfoI GGC'GCC Cuts at: 2561 2809 2561 Size: 248 4881 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5129 SmaI CCC'GGG Cuts at: 1030 2151 1030 Size: 1121 4008 SmlI C'TyrA_G Cuts at: 623 2284 3199 3913 4175 623 Size: 1661 915 714 262 1577 Fragments arranged by size: 1661 1577 915 714 262 SpeI A'CTAG_T Cuts at: 67 889 67 Size: 822 4307 SphI G_CATG'C Cuts at: 2182 2682 2182 Size: 500 4629 SspI AAT'ATT Cuts at: 486 1758 486 Size: 1272 3857 StyI C'CwwG_G Cuts at: 107 2263 2617 107 Size: 2156 354 2619 Fragments arranged by size: 2619 2156 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 305 1012 2712 2845 3304 3513 305 Size: 707 1700 133 459 209 1921 Fragments arranged by size: 1921 1700 707 459 209 133 TatI w'GTAC_w Cuts at: 536 886 1499 1820 4644 536 Size: 350 613 321 2824 1021 Fragments arranged by size: 2824 1021 613 350 321 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 4646 4678 2067 Size: 32 2547 32 2518 Fragments arranged by size: 2547 2518 32 32 Tth111I GACn'n_nGTC Cuts at: 2926 2926 Size: 5129 XbaI T'CTAG_A Cuts at: 603 2160 603 Size: 1557 3572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2624 2624 Size: 5129 XmaI C'CCGG_G Cuts at: 1028 2149 1028 Size: 1121 4008 XmnI GAAnn'nnTTC Cuts at: 486 1649 486 Size: 1163 3966 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AloI AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI