Restriction map for pF1KA0906
 (Linear) (Six-base) MAPSORT of: KIAA0906.seq  Check: 9362  from: 1  to: 5661



KIAA0906.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2313    3471    5028    5661 
   Size:       2313    1158    1557     633

  Fragments arranged by size: 

                2313    1557    1158     633
 
AatII G_ACGT'C

Cuts at:      0     752    3656    5661 
   Size:        752    2904    2005

  Fragments arranged by size: 

                2904    2005     752
 
AccI GT'mk_AC

Cuts at:      0     553     677     752    2270    5661 
   Size:        553     124      75    1518    3391

  Fragments arranged by size: 

                3391    1518     553     124      75
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     430     838    1437    2388    3076    5043    5661 
   Size:        429       1     408     599     951     688    1967     618

  Fragments arranged by size: 

                1967     951     688     618     599     429     408       1
            
 
AfeI AGC'GCT

Cuts at:      0    3369    5661 
   Size:       3369    2292
 
AflIII A'CryG_T

Cuts at:      0     917    1641    2291    2921    5661 
   Size:        917     724     650     630    2740

  Fragments arranged by size: 

                2740     917     724     650     630
 
AleI CACnn'nnGTG

Cuts at:      0    1061    1279    1892    5661 
   Size:       1061     218     613    3769

  Fragments arranged by size: 

                3769    1061     613     218
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2371    3568    3628    5661 
   Size:       2371    1197      60    2033

  Fragments arranged by size: 

                2371    2033    1197      60
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3580    3612    5661 
   Size:       3580      32    2049

  Fragments arranged by size: 

                3580    2049      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     423    1492    1656    1804    2940    5661 
   Size:        318     105    1069     164     148    1136    2721

  Fragments arranged by size: 

                2721    1136    1069     318     164     148     105
 
ApaI G_GGCC'C

Cuts at:      0      61     874    1434    4315    5375    5661 
   Size:         61     813     560    2881    1060     286

  Fragments arranged by size: 

                2881    1060     813     560     286      61
 
ApaLI G'TGCA_C

Cuts at:      0    2836    4773    5661 
   Size:       2836    1937     888

  Fragments arranged by size: 

                2836    1937     888
 
ApoI r'AATT_y

Cuts at:      0    1346    2029    5661 
   Size:       1346     683    3632

  Fragments arranged by size: 

                3632    1346     683
 
AseI AT'TA_AT

Cuts at:      0    3895    5661 
   Size:       3895    1766
 
AvaI C'yCGr_G

Cuts at:      0     867    2017    2850    4389    4580    5501    5534    5661 
   Size:        867    1150     833    1539     191     921      33     127

  Fragments arranged by size: 

                1539    1150     921     867     833     191     127      33
            
 
AvrII C'CTAG_G

Cuts at:      0    1041    5661 
   Size:       1041    4620
 
BamHI G'GATC_C

Cuts at:      0    1938    2012    2120    3512    4591    5661 
   Size:       1938      74     108    1392    1079    1070

  Fragments arranged by size: 

                1938    1392    1079    1070     108      74
 
BanI G'GyrC_C

Cuts at:      0    1317    1408    2640    3544    3674    4622    5052    5137 
   Size:       1317      91    1232     904     130     948     430      85

Cuts at:   5137    5661 
   Size:        524

  Fragments arranged by size: 

                1317    1232     948     904     524     430     130      91
                  85
 
BanII G_rGCy'C

Cuts at:      0      61     383     646     874    1434    1604    1849    1972 
   Size:         61     322     263     228     560     170     245     123

Cuts at:   1972    2666    2695    2741    2857    3422    4073    4147    4315 
   Size:        694      29      46     116     565     651      74     168

Cuts at:   4315    4528    5375    5644    5661 
   Size:        213     847     269      17

  Fragments arranged by size: 

                 847     694     651     565     560     322     269     263
                 245     228     213     170     168     123     116      74
                  61      46      29      17
 
BbeI G_GCGC'C

Cuts at:      0    1412    5661 
   Size:       1412    4249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     459     640    1454    2883    2983    4236    4990    5661 
   Size:        459     181     814    1429     100    1253     754     671

  Fragments arranged by size: 

                1429    1253     814     754     671     459     181     100
            
 
BbvCI CC'TCA_GC

Cuts at:      0    2051    2602    3757    5661 
   Size:       2051     551    1155    1904

  Fragments arranged by size: 

                2051    1904    1155     551
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     270     304    1506    1540    4042    4076    5661 
   Size:        270      34    1202      34    2502      34    1585

  Fragments arranged by size: 

                2502    1585    1202     270      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1030    1379    4122    5661 
   Size:       1030     349    2743    1539

  Fragments arranged by size: 

                2743    1539    1030     349
 
BclI T'GATC_A

Cuts at:      0    5471    5661 
   Size:       5471     190
 
BfrBI ATG'CAT

Cuts at:      0    5596    5661 
   Size:       5596      65
 
BglI GCCn_nnn'nGGC

Cuts at:      0      73    4180    4505    5083    5191    5452    5661 
   Size:         73    4107     325     578     108     261     209

  Fragments arranged by size: 

                4107     578     325     261     209     108      73
 
BlpI GC'TnA_GC

Cuts at:      0    2462    5661 
   Size:       2462    3199
 
Bme1580I G_kGCm'C

Cuts at:      0      61     874    1239    1434    2840    3547    4315    4625 
   Size:         61     813     365     195    1406     707     768     310

Cuts at:   4625    4777    5057    5142    5375    5661 
   Size:        152     280      85     233     286

  Fragments arranged by size: 

                1406     813     768     707     365     310     286     280
                 233     195     152      85      61
 
BmgBI CAC'GTC

Cuts at:      0     920    1884    2682    4470    5661 
   Size:        920     964     798    1788    1191

  Fragments arranged by size: 

                1788    1191     964     920     798
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2326    4833    5332    5661 
   Size:       2326    2507     499     329

  Fragments arranged by size: 

                2507    2326     499     329
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1215    5410    5661 
   Size:       1215    4195     251

  Fragments arranged by size: 

                4195    1215     251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     409     424    1099    1678    4256    4542    4998 
   Size:        160     249      15     675     579    2578     286     456

Cuts at:   4998    5173    5289    5661 
   Size:        175     116     372

  Fragments arranged by size: 

                2578     675     579     456     372     286     249     175
                 160     116      15
 
Bpu10I CC'TnA_GC

Cuts at:      0    2051    2602    2757    3499    3757    4972    5661 
   Size:       2051     551     155     742     258    1215     689

  Fragments arranged by size: 

                2051    1215     742     689     551     258     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1737    1912    4856    5661 
   Size:        637    1100     175    2944     805

  Fragments arranged by size: 

                2944    1100     805     637     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     875    1075    3436    4810    5074    5661 
   Size:        875     200    2361    1374     264     587

  Fragments arranged by size: 

                2361    1374     875     587     264     200
 
BsaAI yAC'GTr

Cuts at:      0     557    1023    1642    2922    5661 
   Size:        557     466     619    1280    2739

  Fragments arranged by size: 

                2739    1280     619     557     466
 
BsaBI GATnn'nnATC

Cuts at:      0    3556    5661 
   Size:       3556    2105
 
BsaHI Gr'CG_yC

Cuts at:      0     749    1409    1820    3653    3685    5249    5661 
   Size:        749     660     411    1833      32    1564     412

  Fragments arranged by size: 

                1833    1564     749     660     412     411      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     541     571    1972    2002    3580    3610    4882    4912 
   Size:        541      30    1401      30    1578      30    1272      30

Cuts at:   4912    5661 
   Size:        749

  Fragments arranged by size: 

                1578    1401    1272     749     541      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1935    1962    4518    5661 
   Size:       1935      27    2556    1143

  Fragments arranged by size: 

                2556    1935    1143      27
 
BseYI C'CCAG_C

Cuts at:      0     204     260     942    1372    2182    2282    2901    3311 
   Size:        204      56     682     430     810     100     619     410

Cuts at:   3311    3373    4618    5224    5455    5643    5661 
   Size:         62    1245     606     231     188      18

  Fragments arranged by size: 

                1245     810     682     619     606     430     410     231
                 204     188     100      62      56      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     808     917    1576    2500    3445    3499    3962 
   Size:        265     543     109     659     924     945      54     463

Cuts at:   3962    4138    4318    5478    5661 
   Size:        176     180    1160     183

  Fragments arranged by size: 

                1160     945     924     659     543     463     265     183
                 180     176     109      54
 
BsiEI CG_ry'CG

Cuts at:      0     181     895    1089    3735    4853    5186    5246    5661 
   Size:        181     714     194    2646    1118     333      60     415

  Fragments arranged by size: 

                2646    1118     714     415     333     194     181      60
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     229     383     646    1199    1811    2666    2741    2840 
   Size:        229     154     263     553     612     855      75      99

Cuts at:   2840    3731    4388    4475    4528    4777    5661 
   Size:        891     657      87      53     249     884

  Fragments arranged by size: 

                 891     884     855     657     612     553     263     249
                 229     154      99      87      75      53
 
BsmI GAATG_Cn'

Cuts at:      0    5194    5661 
   Size:       5194     467
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     369    4280    5256    5661 
   Size:        369    3911     976     405

  Fragments arranged by size: 

                3911     976     405     369
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     229     383     646     874    1199    1239    1434 
   Size:         61     168     154     263     228     325      40     195

Cuts at:   1434    1604    1811    1849    1972    2666    2695    2741    2840 
   Size:        170     207      38     123     694      29      46      99

Cuts at:   2840    2857    3422    3547    3731    4073    4147    4315    4388 
   Size:         17     565     125     184     342      74     168      73

Cuts at:   4388    4475    4528    4625    4777    5057    5142    5375    5644 
   Size:         87      53      97     152     280      85     233     269

Cuts at:   5644    5661 
   Size:         17

  Fragments arranged by size: 

                 694     565     342     325     280     269     263     233
                 228     207     195     184     170     168     168     154
                 152     125     123      99      97      87      85      74
                  73      61      53      46      40      38      29      17
                  17
 
BspHI T'CATG_A

Cuts at:      0    2867    5468    5661 
   Size:       2867    2601     193

  Fragments arranged by size: 

                2867    2601     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1928    2142    2313    3279    3471    5028    5661 
   Size:       1928     214     171     966     192    1557     633

  Fragments arranged by size: 

                1928    1557     966     633     214     192     171
 
BsrDI GCAATG_nn'

Cuts at:      0    1907    3351    3612    5327    5596    5661 
   Size:       1907    1444     261    1715     269      65

  Fragments arranged by size: 

                1907    1715    1444     269     261      65
 
BsrFI r'CCGG_y

Cuts at:      0     894    1084    2390    3445    3568    4848    5661 
   Size:        894     190    1306    1055     123    1280     813

  Fragments arranged by size: 

                1306    1280    1055     894     813     190     123
 
BsrGI T'GTAC_A

Cuts at:      0    2942    5661 
   Size:       2942    2719
 
BssSI C'ACGA_G

Cuts at:      0    2542    3421    3475    3679    5661 
   Size:       2542     879      54     204    1982

  Fragments arranged by size: 

                2542    1982     879     204      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2523    2844    3436    5661 
   Size:       2523     321     592    2225

  Fragments arranged by size: 

                2523    2225     592     321
 
BstEII G'GTnAC_C

Cuts at:      0    1001    1710    1959    2703    3291    5661 
   Size:       1001     709     249     744     588    2370

  Fragments arranged by size: 

                2370    1001     744     709     588     249
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1707    2478    4461    5661 
   Size:       1707     771    1983    1200

  Fragments arranged by size: 

                1983    1707    1200     771
 
BstYI r'GATC_y

Cuts at:      0     413    1938    2012    2120    3512    3830    4591    5505 
   Size:        413    1525      74     108    1392     318     761     914

Cuts at:   5505    5661 
   Size:        156

  Fragments arranged by size: 

                1525    1392     914     761     413     318     156     108
                  74
 
Bsu36I CC'TnA_GG

Cuts at:      0    1669    4129    4627    5628    5661 
   Size:       1669    2460     498    1001      33

  Fragments arranged by size: 

                2460    1669    1001     498      33
 
BtgI C'CryG_G

Cuts at:      0    1390    1434    2219    2831    3138    4454    4586    4739 
   Size:       1390      44     785     612     307    1316     132     153

Cuts at:   4739    4886    5261    5367    5661 
   Size:        147     375     106     294

  Fragments arranged by size: 

                1390    1316     785     612     375     307     294     153
                 147     132     106      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1210    2142    5661 
   Size:       1210     932    3519

  Fragments arranged by size: 

                3519    1210     932
 
BtsI GCAGTG_nn'

Cuts at:      0     229     245     929     934    1449    1524    1809    2567 
   Size:        229      16     684       5     515      75     285     758

Cuts at:   2567    2573    3265    3437    4360    4904    5661 
   Size:          6     692     172     923     544     757

  Fragments arranged by size: 

                 923     758     757     692     684     544     515     285
                 229     172      75      16       6       5
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1842    1877    5661 
   Size:       1842      35    3784

  Fragments arranged by size: 

                3784    1842      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1744    5661 
   Size:       1744    3917
 
EaeI y'GGCC_r

Cuts at:      0      74     178     188     892     966    1086    1256    1878 
   Size:         74     104      10     704      74     120     170     622

Cuts at:   1878    2447    2502    3146    3153    3732    3809    4181    4481 
   Size:        569      55     644       7     579      77     372     300

Cuts at:   4481    4532    4850    4914    5183    5661 
   Size:         51     318      64     269     478

  Fragments arranged by size: 

                 704     644     622     579     569     478     372     318
                 300     269     170     120     104      77      74      74
                  64      55      51      10       7
 
EagI C'GGCC_G

Cuts at:      0     178     892    1086    3732    4850    5183    5661 
   Size:        178     714     194    2646    1118     333     478

  Fragments arranged by size: 

                2646    1118     714     478     333     194     178
 
EarI CTCTTCn'nnn_

Cuts at:      0    2693    5077    5401    5446    5661 
   Size:       2693    2384     324      45     215

  Fragments arranged by size: 

                2693    2384     324     215      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      54     514    5661 
   Size:         54     460    5147

  Fragments arranged by size: 

                5147     460      54
 
EcoICRI GAG'CTC

Cuts at:      0     381     644    2664    2739    4526    5661 
   Size:        381     263    2020      75    1787    1135

  Fragments arranged by size: 

                2020    1787    1135     381     263      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     409     424     429     430     838    1099    1437 
   Size:        160     249      15       5       1     408     261     338

Cuts at:   1437    1678    2388    3076    4256    4542    4998    5043    5173 
   Size:        241     710     688    1180     286     456      45     130

Cuts at:   5173    5289    5661 
   Size:        116     372

  Fragments arranged by size: 

                1180     710     688     456     408     372     338     286
                 261     249     241     160     130     116      45      15
                   5       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2674    3478    4128    5627    5661 
   Size:       2674     804     650    1499      34

  Fragments arranged by size: 

                2674    1499     804     650      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58     315     420     871    1430    1653    1999    3387 
   Size:         58     257     105     451     559     223     346    1388

Cuts at:   3387    3471    4173    4311    4312    4631    5177    5278    5661 
   Size:         84     702     138       1     319     546     101     383

  Fragments arranged by size: 

                1388     702     559     546     451     383     346     319
                 257     223     138     105     101      84      58       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3039    5661 
   Size:       3039    2622
 
HaeII r_GCGC'y

Cuts at:      0    1412    3371    5661 
   Size:       1412    1959    2290

  Fragments arranged by size: 

                2290    1959    1412
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525    1930    1962    1997    2029    3185    3217 
   Size:        493      32    1405      32      35      32    1156      32

Cuts at:   3217    3772    3804    4738    4770    4852    4884    5155    5187 
   Size:        555      32     934      32      82      32     271      32

Cuts at:   5187    5661 
   Size:        474

  Fragments arranged by size: 

                1405    1156     934     555     493     474     271      82
                  35      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     129    2958    5661 
   Size:        129    2829    2703

  Fragments arranged by size: 

                2829    2703     129
 
HpaI GTT'AAC

Cuts at:      0     129    5661 
   Size:        129    5532
 
KasI G'GCGC_C

Cuts at:      0    1408    5661 
   Size:       1408    4253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2906    3347    3717    5661 
   Size:       2906     441     370    1944

  Fragments arranged by size: 

                2906    1944     441     370
 
MscI TGG'CCA

Cuts at:      0     190     968    2449    2504    3148    3811    4483    5661 
   Size:        190     778    1481      55     644     663     672    1178

  Fragments arranged by size: 

                1481    1178     778     672     663     644     190      55
            
 
MslI CAynn'nnrTG

Cuts at:      0     619     922    1061    1279    1389    1574    1892    2671 
   Size:        619     303     139     218     110     185     318     779

Cuts at:   2671    2866    2950    3532    3550    3724    4001    4054    4459 
   Size:        195      84     582      18     174     277      53     405

Cuts at:   4459    5338    5661 
   Size:        879     323

  Fragments arranged by size: 

                 879     779     619     582     405     323     318     303
                 277     218     195     185     174     139     110      84
                  53      18
 
MspA1I CmG'CkG

Cuts at:      0      70     164     204     942    1457    1817    2050    2286 
   Size:         70      94      40     738     515     360     233     236

Cuts at:   2286    2304    2478    3140    3798    3861    4829    4962    5263 
   Size:         18     174     662     658      63     968     133     301

Cuts at:   5263    5464    5661 
   Size:        201     197

  Fragments arranged by size: 

                 968     738     662     658     515     360     301     236
                 233     201     197     174     133      94      70      63
                  40      18
 
NaeI GCC'GGC

Cuts at:      0    4850    5661 
   Size:       4850     811
 
NarI GG'CG_CC

Cuts at:      0    1409    5661 
   Size:       1409    4252
 
NcoI C'CATG_G

Cuts at:      0    2831    4454    5661 
   Size:       2831    1623    1207

  Fragments arranged by size: 

                2831    1623    1207
 
NgoMIV G'CCGG_C

Cuts at:      0    4848    5661 
   Size:       4848     813
 
NsiI A_TGCA'T

Cuts at:      0    5598    5661 
   Size:       5598      63
 
NspI r_CATG'y

Cuts at:      0     987    2295    4000    5661 
   Size:        987    1308    1705    1661

  Fragments arranged by size: 

                1705    1661    1308     987
 
PasI CC'CwG_GG

Cuts at:      0    1783    1842    1965    5661 
   Size:       1783      59     123    3696

  Fragments arranged by size: 

                3696    1783     123      59
 
PciI A'CATG_T

Cuts at:      0    2291    5661 
   Size:       2291    3370
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     423    1611    1656    2009    5661 
   Size:        423    1188      45     353    3652

  Fragments arranged by size: 

                3652    1188     423     353      45
 
PfoI T'CCnGG_A

Cuts at:      0     665    2098    3656    3965    4706    5408    5661 
   Size:        665    1433    1558     309     741     702     253

  Fragments arranged by size: 

                1558    1433     741     702     665     309     253
 
PmlI CAC'GTG

Cuts at:      0    1642    5661 
   Size:       1642    4019
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3580    3612    5661 
   Size:       3580      32    2049

  Fragments arranged by size: 

                3580    2049      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     315    1999    3471    4631    5177    5661 
   Size:        315    1684    1472    1160     546     484

  Fragments arranged by size: 

                1684    1472    1160     546     484     315
 
PshAI GACnn'nnGTC

Cuts at:      0    3699    5464    5661 
   Size:       3699    1765     197

  Fragments arranged by size: 

                3699    1765     197
 
PspOMI G'GGCC_C

Cuts at:      0      57     870    1430    4311    5371    5661 
   Size:         57     813     560    2881    1060     290

  Fragments arranged by size: 

                2881    1060     813     560     290      57
 
PspXI vC'TCGA_Gb

Cuts at:      0    2017    5534    5661 
   Size:       2017    3517     127

  Fragments arranged by size: 

                3517    2017     127
 
PstI C_TGCA'G

Cuts at:      0     699     941    1816    2312    3860    3926    5523    5661 
   Size:        699     242     875     496    1548      66    1597     138

  Fragments arranged by size: 

                1597    1548     875     699     496     242     138      66
            
 
PvuII CAG'CTG

Cuts at:      0     942    1457    2286    2478    3798    3861    4829    5661 
   Size:        942     515     829     192    1320      63     968     832

  Fragments arranged by size: 

                1320     968     942     832     829     515     192      63
            
 
RsrII CG'GwC_CG

Cuts at:      0    3749    5661 
   Size:       3749    1912
 
SacI G_AGCT'C

Cuts at:      0     383     646    2666    2741    4528    5661 
   Size:        383     263    2020      75    1787    1133

  Fragments arranged by size: 

                2020    1787    1133     383     263      75
 
SacII CC_GC'GG

Cuts at:      0    3141    5264    5661 
   Size:       3141    2123     397

  Fragments arranged by size: 

                3141    2123     397
 
SanDI GG'GwC_CC

Cuts at:      0    5177    5661 
   Size:       5177     484
 
SapI GCTCTTCn'nnn_

Cuts at:      0    5446    5661 
   Size:       5446     215
 
ScaI AGT'ACT

Cuts at:      0    4921    5661 
   Size:       4921     740
 
SexAI A'CCwGG_T

Cuts at:      0    1475    5420    5661 
   Size:       1475    3945     241

  Fragments arranged by size: 

                3945    1475     241
 
SfcI C'TryA_G

Cuts at:      0     695     937    1312    1812    1869    2308    3856    3922 
   Size:        695     242     375     500      57     439    1548      66

Cuts at:   3922    4423    4715    5519    5661 
   Size:        501     292     804     142

  Fragments arranged by size: 

                1548     804     695     501     500     439     375     292
                 242     142      66      57
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    4180    5661 
   Size:       4180    1481
 
SfoI GGC'GCC

Cuts at:      0    1410    5661 
   Size:       1410    4251
 
SmaI CCC'GGG

Cuts at:      0     869    2852    4391    4582    5503    5661 
   Size:        869    1983    1539     191     921     158

  Fragments arranged by size: 

                1983    1539     921     869     191     158
 
SmlI C'TyrA_G

Cuts at:      0     652    1716    1927    2017    4871    5534    5661 
   Size:        652    1064     211      90    2854     663     127

  Fragments arranged by size: 

                2854    1064     663     652     211     127      90
 
SnaBI TAC'GTA

Cuts at:      0     557    5661 
   Size:        557    5104
 
SphI G_CATG'C

Cuts at:      0     987    5661 
   Size:        987    4674
 
SrfI GCCC'GGGC

Cuts at:      0    4582    5661 
   Size:       4582    1079
 
SspI AAT'ATT

Cuts at:      0    3893    5661 
   Size:       3893    1768
 
StuI AGG'CCT

Cuts at:      0     145    2140    3620    4508    4804    5661 
   Size:        145    1995    1480     888     296     857

  Fragments arranged by size: 

                1995    1480     888     857     296     145
 
StyI C'CwwG_G

Cuts at:      0     963    1041    1955    2008    2141    2831    2906    4454 
   Size:        963      78     914      53     133     690      75    1548

Cuts at:   4454    5274    5661 
   Size:        820     387

  Fragments arranged by size: 

                1548     963     914     820     690     387     133      78
                  75      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1062    2134    3562    4174    4251    4798    4826    5207 
   Size:       1062    1072    1428     612      77     547      28     381

Cuts at:   5207    5595    5661 
   Size:        388      66

  Fragments arranged by size: 

                1428    1072    1062     612     547     388     381      77
                  66      28
 
TatI w'GTAC_w

Cuts at:      0    1418    2942    3707    4919    5661 
   Size:       1418    1524     765    1212     742

  Fragments arranged by size: 

                1524    1418    1212     765     742
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1353    1385    4187    4219    5661 
   Size:       1353      32    2802      32    1442

  Fragments arranged by size: 

                2802    1442    1353      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      36    5661 
   Size:         36    5625
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1441    2283    2442    4231    4566    5037    5661 
   Size:       1441     842     159    1789     335     471     624

  Fragments arranged by size: 

                1789    1441     842     624     471     335     159
 
XhoI C'TCGA_G

Cuts at:      0    2017    5534    5661 
   Size:       2017    3517     127

  Fragments arranged by size: 

                3517    2017     127
 
XmaI C'CCGG_G

Cuts at:      0     867    2850    4389    4580    5501    5661 
   Size:        867    1983    1539     191     921     160

  Fragments arranged by size: 

                1983    1539     921     867     191     160
 
XmnI GAAnn'nnTTC

Cuts at:      0     442    3022    3230    3592    3983    5661 
   Size:        442    2580     208     362     391    1678

  Fragments arranged by size: 

                2580    1678     442     391     362     208
 
ZraI GAC'GTC

Cuts at:      0     750    3654    5661 
   Size:        750    2904    2007

  Fragments arranged by size: 

                2904    2007     750
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AscI    AsiSI     BaeI
    BglII     BmtI    BsaWI    BsiWI    BspEI    BsrBI   BssHII    BstBI
  BstZ17I     ClaI     DraI   DraIII    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     PacI     PmeI     PsiI     PsrI     PvuI     SalI     SbfI
     SgfI    SgrAI     SpeI     SwaI     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com