Restriction map for pF1KA0864
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# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AscI        AseI        AsnI        Asp718I     
AspI        BalI        BclI        BfrI        BsaBI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco52I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PflFI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SexAI       SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       2950  TCCGGA               T^CCGGA
AgeI          191  ACCGGT               A^CCGGT
AhdI         1120  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1781  AGCGCT               AGC^GCT
ApaI          970  GGGCCC               GGGCC^C
Asp700I        69  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1120  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1835  TGCGCA               TGC^GCA
AvrII        1024  CCTAGG               C^CTAGG
BamHI        1421  GGATCC               G^GATCC
BbeI         2417  GGCGCC               GGCGC^C
BciVI        1329  GTATCC               GTATCC(6/5)
BglII        2220  AGATCT               A^GATCT
BlnI         1024  CCTAGG               C^CTAGG
BlpI         2841  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2841  GCTCAGC              GC^T[ACGT]AGC
BseAI        2950  TCCGGA               T^CCGGA
BsiEI         756  CGACCG               CG[GA][CT]^CG
BsmI         2499  GAATGC               GAATGC(1/-1)
BspEI        2950  TCCGGA               T^CCGGA
BspHI        1433  TCATGA               T^CATGA
BsrDI        2188  GCAATG               GCAATG(2/0)
CelII        2841  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     1120  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1781  AGCGCT               AGC^GCT
FspI         1835  TGCGCA               TGC^GCA
HincII       2392  GTCAAC               GT[CT]^[GA]AC
HindII       2392  GTCAAC               GT[CT]^[GA]AC
KasI         2417  GGCGCC               G^GCGCC
MroI         2950  TCCGGA               T^CCGGA
NarI         2417  GGCGCC               GG^CGCC
NruI         2849  TCGCGA               TCG^CGA
NspI         1309  GCATGT               [GA]CATG^[CT]
PaeR7I       2035  CTCGAG               C^TCGAG
PinAI         191  ACCGGT               A^CCGGT
RcaI         1433  TCATGA               T^CATGA
SbfI         2097  CCTGCAGG             CCTGCA^GG
ScaI         2477  AGTACT               AGT^ACT
SfiI          331  GGCCGCACGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2417  GGCGCC               GGC^GCC
Sse8387I     2097  CCTGCAGG             CCTGCA^GG
XhoI         2035  CTCGAG               C^TCGAG
XmnI           69  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI           31  AAATTC               [GA]^AATT[CT]
             1161  GAATTT              
AocI          280  CCTCAGG              CC^T[ACGT]AGG
             1720  CCTGAGG             
ApoI           31  AAATTC               [GA]^AATT[CT]
             1161  GAATTT              
AvaI         1524  CCCGAG               C^[CT]CG[GA]G
             2035  CTCGAG              
BbsI          813  GAAGAC               GAAGAC(2/6)
             1983  GTCTTC              
BbvCI        1267  GCTGAGG              CCTCAGC(-5/-2)
             2109  GCTGAGG             
BcgI          189  GCACCGGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1778  GCAAGCGCTTCG        
BpuAI         813  GAAGAC               GAAGAC(2/6)
             1983  GTCTTC              
BsgI           24  GTGCAG               GTGCAG(16/14)
             2446  GTGCAG              
BsoBI        1524  CCCGAG               C^[CT]CG[GA]G
             2035  CTCGAG              
BssSI        1705  CACGAG               CACGAG(-5/-1)
             2017  CACGAG              
BstAPI       2439  GCAGTCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2535  GCAGGCCCTGC         
BstEII       1999  GGTGACC              G^GT[ACGT]ACC
             2669  GGTCACC             
BstPI        1999  GGTGACC              G^GT[ACGT]ACC
             2669  GGTCACC             
Bsu36I        280  CCTCAGG              CC^T[ACGT]AGG
             1720  CCTGAGG             
DsaI         1130  CCACGG               C^C[GA][CT]GG
             2942  CCACGG              
Eco81I        280  CCTCAGG              CC^T[ACGT]AGG
             1720  CCTGAGG             
EcoO65I      1999  GGTGACC              G^GT[ACGT]ACC
             2669  GGTCACC             
SacI         2569  GAGCTC               GAGCT^C
             2839  GAGCTC              
SapI         1103  GAAGAGC              GCTCTTC(1/4)
             2364  GAAGAGC             


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       AspHI       
AvaII       BanI        BanII       BbrPI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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