Restriction map for pF1KA0853
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AocI        ApaI        AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbvCI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    Bsp1407I    BsrGI       BssHII      
Bst1107I    BstZ17I     Bsu36I      CelII       CpoI        
DraI        DraII       DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoT22I     FseI        FspI        HincII      
HindII      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MunI        NarI        NcoI        
NdeI        NotI        NruI        NsiI        PacI        
PflFI       PinAI       PmeI        PpuMI       PshAI       
Psp1406I    RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        3931  CGCG                 CG^CG
AccIII       2535  TCCGGA               T^CCGGA
Alw44I       2798  GTGCAC               G^TGCAC
Aor51HI      2471  AGCGCT               AGC^GCT
ApaLI        2798  GTGCAC               G^TGCAC
BalI         3991  TGGCCA               TGG^CCA
BanI         4837  GGCACC               G^G[CT][GA]CC
BbrPI        3514  CACGTG               CAC^GTG
BcgI         3566  GCAGCAGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1577  CCCGG                CC^[GC]GG
BsaWI        2535  TCCGGA               [AT]^CCGG[AT]
BseAI        2535  TCCGGA               T^CCGGA
BsiEI        2199  CGATCG               CG[GA][CT]^CG
BsiWI        1618  CGTACG               C^GTACG
BspDI        3908  ATCGAT               AT^CGAT
BspEI        2535  TCCGGA               T^CCGGA
BsrDI        3137  GCAATG               GCAATG(2/0)
BsrFI        2519  GCCGGC               [GA]^CCGG[CT]
BstBI        4808  TTCGAA               TT^CGAA
BstEII        227  GGTCACC              G^GT[ACGT]ACC
BstPI         227  GGTCACC              G^GT[ACGT]ACC
BstUI        3931  CGCG                 CG^CG
BstXI        3642  CCATCGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       2519  GCCGGC               [GA]^CCGG[CT]
ClaI         3908  ATCGAT               AT^CGAT
DraIII        191  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3091  CCACGG               C^C[GA][CT]GG
Eco47III     2471  AGCGCT               AGC^GCT
EcoO65I       227  GGTCACC              G^GT[ACGT]ACC
HindIII      4850  AAGCTT               A^AGCTT
MluNI        3991  TGGCCA               TGG^CCA
MroI         2535  TCCGGA               T^CCGGA
MscI         3991  TGGCCA               TGG^CCA
MvnI         3931  CGCG                 CG^CG
NaeI         2519  GCCGGC               GCC^GGC
NciI         1577  CCCGG                CC^[GC]GG
NgoMIV       2519  GCCGGC               G^CCGGC
NheI          688  GCTAGC               G^CTAGC
NspV         4808  TTCGAA               TT^CGAA
PaeR7I       1355  CTCGAG               C^TCGAG
PmaCI        3514  CACGTG               CAC^GTG
PmlI         3514  CACGTG               CAC^GTG
PstI          713  CTGCAG               CTGCA^G
PvuI         2199  CGATCG               CGAT^CG
PvuII        1782  CAGCTG               CAG^CTG
SapI         1322  GCTCTTC              GCTCTTC(1/4)
SfuI         4808  TTCGAA               TT^CGAA
SgrAI        2518  CGCCGGCG             C[GA]^CCGG[CT]G
XcmI         3858  CCATTCAAGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1355  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI         1235  GACGCC               G[GA]^CG[CT]C
             3507  GACGCC              
AflIII        166  ACATGT               A^C[GA][CT]GT
             4642  ACATGT              
AseI          466  ATTAAT               AT^TAAT
              528  ATTAAT              
AsnI          466  ATTAAT               AT^TAAT
              528  ATTAAT              
BclI         1203  TGATCA               T^GATCA
             3753  TGATCA              
BglII        3865  AGATCT               A^GATCT
             4257  AGATCT              
BsaAI         782  CACGTA               [CT]AC^GT[GA]
             3514  CACGTG              
BsaHI        1235  GACGCC               G[GA]^CG[CT]C
             3507  GACGCC              
BsmBI        1139  CGTCTC               CGTCTC(1/5)
             3505  GAGACG              
BsmI         2792  GAATGC               GAATGC(1/-1)
             3187  GCATTC              
BspLU11I      166  ACATGT               A^CATGT
             4642  ACATGT              
BspMI        4420  GCAGGT               ACCTGC(4/8)
             4609  GCAGGT              
BsrBI        2062  GAGCGG               CCGCTC(-3/-3)
             2206  GAGCGG              
BstAPI       3349  GCAACTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4420  GCAGGTGATGC         
EaeI         1587  TGGCCG               [CT]^GGCC[GA]
             3991  TGGCCA              
EcoRV         817  GATATC               GAT^ATC
             1820  GATATC              
EcoT14I       940  CCAAGG               C^C[AT][AT]GG
             4232  CCTTGG              
FbaI         1203  TGATCA               T^GATCA
             3753  TGATCA              
Hin1I        1235  GACGCC               G[GA]^CG[CT]C
             3507  GACGCC              
MspA1I       1078  CAGCGG               C[AC]G^C[GT]G
             1782  CAGCTG              
PflMI         279  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2437  CCAAGAGATGG         
PshBI         466  ATTAAT               AT^TAAT
              528  ATTAAT              
SacI         1320  GAGCTC               GAGCT^C
             2852  GAGCTC              
ScaI         3678  AGTACT               AGT^ACT
             3784  AGTACT              
StyI          940  CCAAGG               C^C[AT][AT]GG
             4232  CCTTGG              
Van91I        279  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2437  CCAAGAGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BciVI       BfaI        BmyI        BpmI        BpuAI       
BsaBI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XhoII       XmnI        
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