Restriction map for pF1KA0853
 (Linear) (Six-base) MAPSORT of: KIAA0853.seq  Check: 8579  from: 1  to: 5004



KIAA0853.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4411    5004 
   Size:       4411     593
 
AccI GT'mk_AC

Cuts at:      0     946    1994    4874    5004 
   Size:        946    1048    2880     130

  Fragments arranged by size: 

                2880    1048     946     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     668    2849    2978    3776    4773    4973    5004 
   Size:        157     511    2181     129     798     997     200      31

  Fragments arranged by size: 

                2181     997     798     511     200     157     129      31
            
 
AfeI AGC'GCT

Cuts at:      0    2473    5004 
   Size:       2473    2531
 
AflIII A'CryG_T

Cuts at:      0     166    4642    5004 
   Size:        166    4476     362

  Fragments arranged by size: 

                4476     362     166
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2685    2717    5004 
   Size:       2685      32    2287

  Fragments arranged by size: 

                2685    2287      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3197    4615    4992    5004 
   Size:       3197    1418     377      12

  Fragments arranged by size: 

                3197    1418     377      12
 
ApaLI G'TGCA_C

Cuts at:      0    2798    5004 
   Size:       2798    2206
 
ApoI r'AATT_y

Cuts at:      0     747    2882    4412    4558    5004 
   Size:        747    2135    1530     146     446

  Fragments arranged by size: 

                2135    1530     747     446     146
 
AseI AT'TA_AT

Cuts at:      0     467     529    5004 
   Size:        467      62    4475

  Fragments arranged by size: 

                4475     467      62
 
AvaI C'yCGr_G

Cuts at:      0    1355    3574    4217    5004 
   Size:       1355    2219     643     787

  Fragments arranged by size: 

                2219    1355     787     643
 
BanI G'GyrC_C

Cuts at:      0    4837    5004 
   Size:       4837     167
 
BanII G_rGCy'C

Cuts at:      0     299    1324    2856    4707    5004 
   Size:        299    1025    1532    1851     297

  Fragments arranged by size: 

                1851    1532    1025     299     297
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      72     145     380     674     712    1878    2378    2654 
   Size:         72      73     235     294      38    1166     500     276

Cuts at:   2654    3467    3607    4741    4891    4924    5004 
   Size:        813     140    1134     150      33      80

  Fragments arranged by size: 

                1166    1134     813     500     294     276     235     150
                 140      80      73      72      38      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3555    3589    5004 
   Size:       3555      34    1415

  Fragments arranged by size: 

                3555    1415      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1551    4372    4990    5004 
   Size:       1551    2821     618      14

  Fragments arranged by size: 

                2821    1551     618      14
 
BclI T'GATC_A

Cuts at:      0    1203    3753    5004 
   Size:       1203    2550    1251

  Fragments arranged by size: 

                2550    1251    1203
 
BglII A'GATC_T

Cuts at:      0    3865    4257    5004 
   Size:       3865     392     747

  Fragments arranged by size: 

                3865     747     392
 
Bme1580I G_kGCm'C

Cuts at:      0    2802    4840    5004 
   Size:       2802    2038     164

  Fragments arranged by size: 

                2802    2038     164
 
BmgBI CAC'GTC

Cuts at:      0     285    5004 
   Size:        285    4719
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      65    3770    5004 
   Size:         65    3705    1234

  Fragments arranged by size: 

                3705    1234      65
 
BmtI G_CTAG'C

Cuts at:      0     692    5004 
   Size:        692    4312
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342    5004 
   Size:        342    4662
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386    1401    3821    4391    4589    4919    5004 
   Size:        386    1015    2420     570     198     330      85

  Fragments arranged by size: 

                2420    1015     570     386     330     198      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1272    1419    1716    1981    2594    2610    2713    2772 
   Size:       1272     147     297     265     613      16     103      59

Cuts at:   2772    3678    5004 
   Size:        906    1326

  Fragments arranged by size: 

                1326    1272     906     613     297     265     147     103
                  59      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1325    1880    2180    2186    5004 
   Size:       1325     555     300       6    2818

  Fragments arranged by size: 

                2818    1325     555     300       6
 
BsaAI yAC'GTr

Cuts at:      0     784    3516    5004 
   Size:        784    2732    1488

  Fragments arranged by size: 

                2732    1488     784
 
BsaBI GATnn'nnATC

Cuts at:      0    1544    1562    1820    2432    4256    5004 
   Size:       1544      18     258     612    1824     748

  Fragments arranged by size: 

                1824    1544     748     612     258      18
 
BsaHI Gr'CG_yC

Cuts at:      0    1236    3508    5004 
   Size:       1236    2272    1496

  Fragments arranged by size: 

                2272    1496    1236
 
BsaWI w'CCGG_w

Cuts at:      0    2535    5004 
   Size:       2535    2469
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1074    1104    3267    3297    3579    3609    4848    4878 
   Size:       1074      30    2163      30     282      30    1239      30

Cuts at:   4878    5004 
   Size:        126

  Fragments arranged by size: 

                2163    1239    1074     282     126      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     331    1034    2686    3275    3296    3576    3957    4471 
   Size:        331     703    1652     589      21     280     381     514

Cuts at:   4471    5004 
   Size:        533

  Fragments arranged by size: 

                1652     703     589     533     514     381     331     280
                  21
 
BseYI C'CCAG_C

Cuts at:      0      94     954    1784    2494    3065    5004 
   Size:         94     860     830     710     571    1939

  Fragments arranged by size: 

                1939     860     830     710     571      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3667    4291    4532    5004 
   Size:       3667     624     241     472

  Fragments arranged by size: 

                3667     624     472     241
 
BsiEI CG_ry'CG

Cuts at:      0    2202    5004 
   Size:       2202    2802
 
BsiHKAI G_wGCw'C

Cuts at:      0    1324    2802    2856    4866    5004 
   Size:       1324    1478      54    2010     138

  Fragments arranged by size: 

                2010    1478    1324     138      54
 
BsiWI C'GTAC_G

Cuts at:      0    1618    5004 
   Size:       1618    3386
 
BsmI GAATG_Cn'

Cuts at:      0    2798    3187    5004 
   Size:       2798     389    1817

  Fragments arranged by size: 

                2798    1817     389
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1145    3499    5004 
   Size:       1145    2354    1505

  Fragments arranged by size: 

                2354    1505    1145
 
Bsp1286I G_dGCh'C

Cuts at:      0     299    1324    2802    2856    4707    4840    4866    5004 
   Size:        299    1025    1478      54    1851     133      26     138

  Fragments arranged by size: 

                1851    1478    1025     299     138     133      54      26
            
 
BspEI T'CCGG_A

Cuts at:      0    2535    5004 
   Size:       2535    2469
 
BspHI T'CATG_A

Cuts at:      0     483    1206    1227    1776    2856    5004 
   Size:        483     723      21     549    1080    2148

  Fragments arranged by size: 

                2148    1080     723     549     483      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4411    4600    5004 
   Size:       4411     189     404

  Fragments arranged by size: 

                4411     404     189
 
BsrBI CCG'CTC

Cuts at:      0    2064    2208    5004 
   Size:       2064     144    2796

  Fragments arranged by size: 

                2796    2064     144
 
BsrDI GCAATG_nn'

Cuts at:      0    3144    5004 
   Size:       3144    1860
 
BsrFI r'CCGG_y

Cuts at:      0    2519    5004 
   Size:       2519    2485
 
BssSI C'ACGA_G

Cuts at:      0     194    1502    3488    4532    4857    5004 
   Size:        194    1308    1986    1044     325     147

  Fragments arranged by size: 

                1986    1308    1044     325     194     147
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3355    4426    5004 
   Size:       3355    1071     578

  Fragments arranged by size: 

                3355    1071     578
 
BstBI TT'CG_AA

Cuts at:      0    4809    5004 
   Size:       4809     195
 
BstEII G'GTnAC_C

Cuts at:      0     227    5004 
   Size:        227    4777
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3649    5004 
   Size:       3649    1355
 
BstYI r'GATC_y

Cuts at:      0     249    2417    3865    4200    4257    4264    4470    5004 
   Size:        249    2168    1448     335      57       7     206     534

  Fragments arranged by size: 

                2168    1448     534     335     249     206      57       7
            
 
BtgI C'CryG_G

Cuts at:      0    3091    5004 
   Size:       3091    1913
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1156    3523    5004 
   Size:       1156    2367    1481

  Fragments arranged by size: 

                2367    1481    1156
 
BtsI GCAGTG_nn'

Cuts at:      0    3329    5004 
   Size:       3329    1675
 
ClaI AT'CG_AT

Cuts at:      0    3909    5004 
   Size:       3909    1095
 
DraIII CAC_nnn'GTG

Cuts at:      0     196    5004 
   Size:        196    4808
 
EaeI y'GGCC_r

Cuts at:      0    1587    3991    5004 
   Size:       1587    2404    1013

  Fragments arranged by size: 

                2404    1587    1013
 
EarI CTCTTCn'nnn_

Cuts at:      0     553    1329    1971    1983    2255    2408    3302    5004 
   Size:        553     776     642      12     272     153     894    1702

  Fragments arranged by size: 

                1702     894     776     642     553     272     153      12
            
 
EcoICRI GAG'CTC

Cuts at:      0    1322    2854    5004 
   Size:       1322    1532    2150

  Fragments arranged by size: 

                2150    1532    1322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     386     668    1401    2849    2978    3776    3821 
   Size:        157     229     282     733    1448     129     798      45

Cuts at:   3821    4391    4589    4773    4919    4973    5004 
   Size:        570     198     184     146      54      31

  Fragments arranged by size: 

                1448     798     733     570     282     229     198     184
                 157     146     129      54      45      31
 
EcoRV GAT'ATC

Cuts at:      0     819    1822    5004 
   Size:        819    1003    3182

  Fragments arranged by size: 

                3182    1003     819
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     965    1079    1874    3806    5004 
   Size:        965     114     795    1932    1198

  Fragments arranged by size: 

                1932    1198     965     795     114
 
HaeII r_GCGC'y

Cuts at:      0     279    2475    2520    2712    5004 
   Size:        279    2196      45     192    2292

  Fragments arranged by size: 

                2292    2196     279     192      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75     307     339    1074    1106    1188    1220 
   Size:         43      32     232      32     735      32      82      32

Cuts at:   1220    1306    1338    1464    1480    1496    1512    2402    2434 
   Size:         86      32     126      16      16      16     890      32

Cuts at:   2434    2832    2864    2938    2970    3727    3759    4249    4281 
   Size:        398      32      74      32     757      32     490      32

Cuts at:   4281    4315    4347    4455    4487    5004 
   Size:         34      32     108      32     517

  Fragments arranged by size: 

                 890     757     735     517     490     398     232     126
                 108      86      82      74      43      34      32      32
                  32      32      32      32      32      32      32      32
                  32      32      16      16      16
 
HindIII A'AGCT_T

Cuts at:      0    4850    5004 
   Size:       4850     154
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    2904    5004 
   Size:        713    2191    2100

  Fragments arranged by size: 

                2191    2100     713
 
MscI TGG'CCA

Cuts at:      0    3993    5004 
   Size:       3993    1011
 
MslI CAynn'nnrTG

Cuts at:      0    1205    1340    3647    5004 
   Size:       1205     135    2307    1357

  Fragments arranged by size: 

                2307    1357    1205     135
 
MspA1I CmG'CkG

Cuts at:      0    1080    1784    5004 
   Size:       1080     704    3220

  Fragments arranged by size: 

                3220    1080     704
 
NaeI GCC'GGC

Cuts at:      0    2521    5004 
   Size:       2521    2483
 
NgoMIV G'CCGG_C

Cuts at:      0    2519    5004 
   Size:       2519    2485
 
NheI G'CTAG_C

Cuts at:      0     688    5004 
   Size:        688    4316
 
NspI r_CATG'y

Cuts at:      0     170    1428    4646    5004 
   Size:        170    1258    3218     358

  Fragments arranged by size: 

                3218    1258     358     170
 
PciI A'CATG_T

Cuts at:      0     166    4642    5004 
   Size:        166    4476     362

  Fragments arranged by size: 

                4476     362     166
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     285    2443    5004 
   Size:        285    2158    2561

  Fragments arranged by size: 

                2561    2158     285
 
PfoI T'CCnGG_A

Cuts at:      0    1576    2777    4935    5004 
   Size:       1576    1201    2158      69

  Fragments arranged by size: 

                2158    1576    1201      69
 
PmlI CAC'GTG

Cuts at:      0    3516    5004 
   Size:       3516    1488
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2711    2743    5004 
   Size:       2711      32    2261

  Fragments arranged by size: 

                2711    2261      32
 
PstI C_TGCA'G

Cuts at:      0     717    5004 
   Size:        717    4287
 
PvuI CG_AT'CG

Cuts at:      0    2202    5004 
   Size:       2202    2802
 
PvuII CAG'CTG

Cuts at:      0    1784    5004 
   Size:       1784    3220
 
SacI G_AGCT'C

Cuts at:      0    1324    2856    5004 
   Size:       1324    1532    2148

  Fragments arranged by size: 

                2148    1532    1324
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1329    5004 
   Size:       1329    3675
 
ScaI AGT'ACT

Cuts at:      0    3680    3786    5004 
   Size:       3680     106    1218

  Fragments arranged by size: 

                3680    1218     106
 
SfcI C'TryA_G

Cuts at:      0     242     713    1518    2475    2747    3521    5004 
   Size:        242     471     805     957     272     774    1483

  Fragments arranged by size: 

                1483     957     805     774     471     272     242
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2519    5004 
   Size:       2519    2485
 
SmlI C'TyrA_G

Cuts at:      0    1287    1355    1434    1731    1960    2589    2609    2728 
   Size:       1287      68      79     297     229     629      20     119

Cuts at:   2728    2787    3693    5004 
   Size:         59     906    1311

  Fragments arranged by size: 

                1311    1287     906     629     297     229     119      79
                  68      59      20
 
StyI C'CwwG_G

Cuts at:      0     940    4232    5004 
   Size:        940    3292     772

  Fragments arranged by size: 

                3292     940     772
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1412    1484    1688    2204    3986    5004 
   Size:       1412      72     204     516    1782    1018

  Fragments arranged by size: 

                1782    1412    1018     516     204      72
 
TatI w'GTAC_w

Cuts at:      0     106    3678    3784    5004 
   Size:        106    3572     106    1220

  Fragments arranged by size: 

                3572    1220     106     106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     985    1017    4848    4880    5004 
   Size:        985      32    3831      32     124

  Fragments arranged by size: 

                3831     985     124      32      32
 
XbaI T'CTAG_A

Cuts at:      0     985    1327    3156    4203    5004 
   Size:        985     342    1829    1047     801

  Fragments arranged by size: 

                1829    1047     985     801     342
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3865    5004 
   Size:       3865    1139
 
XhoI C'TCGA_G

Cuts at:      0    1355    5004 
   Size:       1355    3649
 
XmnI GAAnn'nnTTC

Cuts at:      0    1062    3786    3831    4886    5004 
   Size:       1062    2724      45    1055     118

  Fragments arranged by size: 

                2724    1062    1055     118      45
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AloI    AlwNI    ApaLI
     ApoI     AseI     AvaI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoRV     FalI    HaeII
    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NheI     NspI     PciI    PflMI     PfoI     PmlI     PpiI     PstI
     PvuI    PvuII     SacI     SapI     ScaI     SfcI    SgrAI     SmlI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     ApaI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BfrBI     BglI     BlpI   Bpu10I    BsrGI   BssHII  BstZ17I   Bsu36I
    CspCI     DraI     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PasI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XmaI     ZraI
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