Restriction map for pF1KA0830
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        Asp700I     AspEI       AviII       AvrII       
BciVI       BclI        BfrI        BlnI        BsaBI       
BsaI        BsgI        BsiWI       BsmBI       BspHI       
BspLU11I    BsrBI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CpoI        DraI        DrdI        Eam1105I    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        KspI        MamI        
MluI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

Acc65I       1414  GGTACC               G^GTACC
AccI          936  GTCTAC               GT^[AC][GT]AC
AccIII       1238  TCCGGA               T^CCGGA
AcsI          483  AAATTC               [GA]^AATT[CT]
AcyI          268  GGCGCC               G[GA]^CG[CT]C
Aor51HI       188  AGCGCT               AGC^GCT
ApoI          483  AAATTC               [GA]^AATT[CT]
Asp718I      1414  GGTACC               G^GTACC
AspHI        1201  GTGCTC               G[AT]GC[AT]^C
AspI          498  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1241  GGATCC               G^GATCC
BbeI          268  GGCGCC               GGCGC^C
BbrPI         154  CACGTG               CAC^GTG
BbsI          580  GAAGAC               GAAGAC(2/6)
BbvCI         784  CCTCAGC              CCTCAGC(-5/-2)
BcgI           29  GCAGCCTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         580  GAAGAC               GAAGAC(2/6)
BsaAI         154  CACGTG               [CT]AC^GT[GA]
BsaHI         268  GGCGCC               G[GA]^CG[CT]C
BsaWI        1238  TCCGGA               [AT]^CCGG[AT]
BseAI        1238  TCCGGA               T^CCGGA
BsiEI         143  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1201  GTGCTC               G[AT]GC[AT]^C
BsmAI        1387  GTCTC                GTCTC(1/5)
BsmI         1187  GCATTC               GAATGC(1/-1)
Bsp1407I     1325  TGTACA               T^GTACA
BspDI         301  ATCGAT               AT^CGAT
BspEI        1238  TCCGGA               T^CCGGA
BspMI        1098  GCAGGT               ACCTGC(4/8)
BsrGI        1325  TGTACA               T^GTACA
BsrI         1368  ACTGG                ACTGG(1/-1)
BssSI          67  CTCGTG               CACGAG(-5/-1)
ClaI          301  ATCGAT               AT^CGAT
DraII         121  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        563  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          143  CGGCCG               C^GGCCG
EclXI         143  CGGCCG               C^GGCCG
Eco47III      188  AGCGCT               AGC^GCT
Eco52I        143  CGGCCG               C^GGCCG
EcoO109I      121  GGGACCC              [GA]G^G[ACGT]CC[CT]
Hin1I         268  GGCGCC               G[GA]^CG[CT]C
HincII        856  GTTAAC               GT[CT]^[GA]AC
HindII        856  GTTAAC               GT[CT]^[GA]AC
HindIII      1021  AAGCTT               A^AGCTT
HpaI          856  GTTAAC               GTT^AAC
KasI          268  GGCGCC               G^GCGCC
KpnI         1414  GGTACC               GGTAC^C
MaeII         155  ACGT                 A^CGT
MfeI         1346  CAATTG               C^AATTG
MroI         1238  TCCGGA               T^CCGGA
MspA1I        787  CAGCTG               C[AC]G^C[GT]G
MunI         1346  CAATTG               C^AATTG
NarI          268  GGCGCC               GG^CGCC
PflFI         498  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         562  CCACAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          498  GACTC                GAGTC(4/5)
PmaCI         154  CACGTG               CAC^GTG
PmlI          154  CACGTG               CAC^GTG
PpuMI         121  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         787  CAGCTG               CAG^CTG
SfoI          268  GGCGCC               GGC^GCC
SspBI        1325  TGTACA               T^GTACA
TaqI          302  TCGA                 T^CGA
Tth111I       498  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       302  TCGA                 T^CGA
Van91I        562  CCACAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwI         1242  GATCC                GGATC(4/5)
             1241  GGATC               
AlwNI         641  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              763  CAGAAACTG           
AvaI          212  CCCGGG               C^[CT]CG[GA]G
              719  CCCGGG              
BanII        1137  GGGCTC               G[GA]GC[CT]^C
             1229  GGGCTC              
BfaI          905  CTAG                 C^TAG
              917  CTAG                
BglI          129  GCCTGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1373  GCCTTGGTGGC         
BglII         161  AGATCT               A^GATCT
              756  AGATCT              
BlpI          184  GCTGAGC              GC^T[ACGT]AGC
             1139  GCTCAGC             
BpmI          950  CTCCAG               CTGGAG(16/14)
              683  CTGGAG              
Bpu1102I      184  GCTGAGC              GC^T[ACGT]AGC
             1139  GCTCAGC             
BsoBI         212  CCCGGG               C^[CT]CG[GA]G
              719  CCCGGG              
BsrDI        1002  CATTGC               GCAATG(2/0)
             1362  CATTGC              
BsrFI          62  GCCGGC               [GA]^CCGG[CT]
               85  GCCGGC              
BssHII         10  GCGCGC               G^CGCGC
              171  GCGCGC              
CelII         184  GCTGAGC              GC^T[ACGT]AGC
             1139  GCTCAGC             
Cfr10I         62  GCCGGC               [GA]^CCGG[CT]
               85  GCCGGC              
DsaI          698  CCATGG               C^C[GA][CT]GG
             1331  CCATGG              
EarI           34  CTCTTC               CTCTTC(1/4)
             1174  GAAGAG              
Ksp632I        34  CTCTTC               CTCTTC(1/4)
             1174  GAAGAG              
MaeI          905  CTAG                 C^TAG
              917  CTAG                
MslI          222  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              354  CACCTCTGTG          
NaeI           62  GCCGGC               GCC^GGC
               85  GCCGGC              
NcoI          698  CCATGG               C^CATGG
             1331  CCATGG              
NgoMIV         62  GCCGGC               G^CCGGC
               85  GCCGGC              
SfcI          204  CTACAG               C^T[GA][CT]AG
              618  CTACAG              
SmaI          212  CCCGGG               CCC^GGG
              719  CCCGGG              
SmlI          322  CTTGAG               C^T[CT][GA]AG
             1204  CTCAAG              
Tsp45I        101  GTGAC                ^GT[GC]AC
              728  GTGAC               
XmaCI         212  CCCGGG               C^CCGGG
              719  CCCGGG              
XmaI          212  CCCGGG               C^CCGGG
              719  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AseI        
AsnI        AvaII       BalI        BanI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoNI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PshBI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       
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