Restriction map for pF1KA0830
 (Linear) (Six-base) MAPSORT of: KIAA0830.seq  Check: 4650  from: 1  to: 1500



KIAA0830.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1089    1500 
   Size:       1089     411
 
AccI GT'mk_AC

Cuts at:      0     937    1500 
   Size:        937     563
 
Acc65I G'GTAC_C

Cuts at:      0    1414    1500 
   Size:       1414      86
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1081    1279    1500 
   Size:        745     336     198     221

  Fragments arranged by size: 

                 745     336     221     198
 
AfeI AGC'GCT

Cuts at:      0     190    1500 
   Size:        190    1310
 
AleI CACnn'nnGTG

Cuts at:      0     358    1500 
   Size:        358    1142
 
AlwNI CAG_nnn'CTG

Cuts at:      0     646     768    1500 
   Size:        646     122     732

  Fragments arranged by size: 

                 732     646     122
 
ApoI r'AATT_y

Cuts at:      0     483    1500 
   Size:        483    1017
 
AseI AT'TA_AT

Cuts at:      0     335    1033    1106    1500 
   Size:        335     698      73     394

  Fragments arranged by size: 

                 698     394     335      73
 
AvaI C'yCGr_G

Cuts at:      0     212     719    1500 
   Size:        212     507     781

  Fragments arranged by size: 

                 781     507     212
 
BamHI G'GATC_C

Cuts at:      0    1241    1500 
   Size:       1241     259
 
BanI G'GyrC_C

Cuts at:      0       4     268    1414    1500 
   Size:          4     264    1146      86

  Fragments arranged by size: 

                1146     264      86       4
 
BanII G_rGCy'C

Cuts at:      0    1141    1233    1500 
   Size:       1141      92     267

  Fragments arranged by size: 

                1141     267      92
 
BbeI G_GCGC'C

Cuts at:      0     272    1500 
   Size:        272    1228
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     587    1500 
   Size:        587     913
 
BbvCI CC'TCA_GC

Cuts at:      0     785    1500 
   Size:        785     715
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      18      52    1500 
   Size:         18      34    1448

  Fragments arranged by size: 

                1448      34      18
 
BglI GCCn_nnn'nGGC

Cuts at:      0     135    1379    1500 
   Size:        135    1244     121

  Fragments arranged by size: 

                1244     135     121
 
BglII A'GATC_T

Cuts at:      0     161     756    1500 
   Size:        161     595     744

  Fragments arranged by size: 

                 744     595     161
 
BlpI GC'TnA_GC

Cuts at:      0     185    1140    1500 
   Size:        185     955     360

  Fragments arranged by size: 

                 955     360     185
 
Bme1580I G_kGCm'C

Cuts at:      0       7     611    1500 
   Size:          7     604     889

  Fragments arranged by size: 

                 889     604       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1378    1500 
   Size:       1378     122
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     956    1500 
   Size:        956     544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     935    1500 
   Size:        704     231     565

  Fragments arranged by size: 

                 704     565     231
 
Bpu10I CC'TnA_GC

Cuts at:      0     445     785    1500 
   Size:        445     340     715

  Fragments arranged by size: 

                 715     445     340
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343    1189    1500 
   Size:        343     846     311

  Fragments arranged by size: 

                 846     343     311
 
BsaAI yAC'GTr

Cuts at:      0     156    1500 
   Size:        156    1344
 
BsaHI Gr'CG_yC

Cuts at:      0     269    1500 
   Size:        269    1231
 
BsaWI w'CCGG_w

Cuts at:      0    1238    1500 
   Size:       1238     262
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     424     454    1500 
   Size:        424      30    1046

  Fragments arranged by size: 

                1046     424      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     340     701    1225    1500 
   Size:         91     249     361     524     275

  Fragments arranged by size: 

                 524     361     275     249      91
 
BseYI C'CCAG_C

Cuts at:      0      24      46     692    1500 
   Size:         24      22     646     808

  Fragments arranged by size: 

                 808     646      24      22
 
BsiEI CG_ry'CG

Cuts at:      0     146    1500 
   Size:        146    1354
 
BsiHKAI G_wGCw'C

Cuts at:      0    1205    1500 
   Size:       1205     295
 
BsmI GAATG_Cn'

Cuts at:      0    1187    1500 
   Size:       1187     313
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     611    1141    1205    1233    1500 
   Size:          7     604     530      64      28     267

  Fragments arranged by size: 

                 604     530     267      64      28       7
 
BspEI T'CCGG_A

Cuts at:      0    1238    1500 
   Size:       1238     262
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1089    1500 
   Size:       1089     411
 
BsrDI GCAATG_nn'

Cuts at:      0    1001    1361    1500 
   Size:       1001     360     139

  Fragments arranged by size: 

                1001     360     139
 
BsrFI r'CCGG_y

Cuts at:      0      62      85    1500 
   Size:         62      23    1415

  Fragments arranged by size: 

                1415      62      23
 
BsrGI T'GTAC_A

Cuts at:      0    1325    1500 
   Size:       1325     175
 
BssHII G'CGCG_C

Cuts at:      0      10     171    1500 
   Size:         10     161    1329

  Fragments arranged by size: 

                1329     161      10
 
BssSI C'ACGA_G

Cuts at:      0      67    1500 
   Size:         67    1433
 
BstYI r'GATC_y

Cuts at:      0     161     756    1241    1500 
   Size:        161     595     485     259

  Fragments arranged by size: 

                 595     485     259     161
 
BtgI C'CryG_G

Cuts at:      0     698    1331    1500 
   Size:        698     633     169

  Fragments arranged by size: 

                 698     633     169
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     294    1500 
   Size:        294    1206
 
BtsI GCAGTG_nn'

Cuts at:      0     456     579    1390    1403    1500 
   Size:        456     123     811      13      97

  Fragments arranged by size: 

                 811     456     123      97      13
 
ClaI AT'CG_AT

Cuts at:      0     302    1500 
   Size:        302    1198
 
DraIII CAC_nnn'GTG

Cuts at:      0     568    1500 
   Size:        568     932
 
EaeI y'GGCC_r

Cuts at:      0     143     464    1163    1214    1310    1500 
   Size:        143     321     699      51      96     190

  Fragments arranged by size: 

                 699     321     190     143      96      51
 
EagI C'GGCC_G

Cuts at:      0     143    1500 
   Size:        143    1357
 
EarI CTCTTCn'nnn_

Cuts at:      0      40    1169    1500 
   Size:         40    1129     331

  Fragments arranged by size: 

                1129     331      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     745     935    1081    1279    1500 
   Size:        704      41     190     146     198     221

  Fragments arranged by size: 

                 704     221     198     190     146      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     325     686    1432    1500 
   Size:        325     361     746      68

  Fragments arranged by size: 

                 746     361     325      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122    1500 
   Size:        122    1378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     402    1500 
   Size:        402    1098
 
HaeII r_GCGC'y

Cuts at:      0     192     272    1340    1500 
   Size:        192      80    1068     160

  Fragments arranged by size: 

                1068     192     160      80
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     891     923    1500 
   Size:        891      32     577

  Fragments arranged by size: 

                 891     577      32
 
HincII GTy'rAC

Cuts at:      0     858    1500 
   Size:        858     642
 
HindIII A'AGCT_T

Cuts at:      0    1021    1500 
   Size:       1021     479
 
HpaI GTT'AAC

Cuts at:      0     858    1500 
   Size:        858     642
 
KasI G'GCGC_C

Cuts at:      0     268    1500 
   Size:        268    1232
 
KpnI G_GTAC'C

Cuts at:      0    1418    1500 
   Size:       1418      82
 
MfeI C'AATT_G

Cuts at:      0    1346    1500 
   Size:       1346     154
 
MscI TGG'CCA

Cuts at:      0     466    1165    1216    1312    1500 
   Size:        466     699      51      96     188

  Fragments arranged by size: 

                 699     466     188      96      51
 
MslI CAynn'nnrTG

Cuts at:      0     226     358    1500 
   Size:        226     132    1142

  Fragments arranged by size: 

                1142     226     132
 
MspA1I CmG'CkG

Cuts at:      0     789    1500 
   Size:        789     711
 
NaeI GCC'GGC

Cuts at:      0      64      87    1500 
   Size:         64      23    1413

  Fragments arranged by size: 

                1413      64      23
 
NarI GG'CG_CC

Cuts at:      0     269    1500 
   Size:        269    1231
 
NcoI C'CATG_G

Cuts at:      0     698    1331    1500 
   Size:        698     633     169

  Fragments arranged by size: 

                 698     633     169
 
NgoMIV G'CCGG_C

Cuts at:      0      62      85    1500 
   Size:         62      23    1415

  Fragments arranged by size: 

                1415      62      23
 
PasI CC'CwG_GG

Cuts at:      0    1419    1500 
   Size:       1419      81
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     568    1500 
   Size:        568     932
 
PfoI T'CCnGG_A

Cuts at:      0     509     845     866    1500 
   Size:        509     336      21     634

  Fragments arranged by size: 

                 634     509     336      21
 
PmlI CAC'GTG

Cuts at:      0     156    1500 
   Size:        156    1344
 
PpuMI rG'GwC_Cy

Cuts at:      0     122    1500 
   Size:        122    1378
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     223     255     869     901    1500 
   Size:        223      32     614      32     599

  Fragments arranged by size: 

                 614     599     223      32      32
 
PvuII CAG'CTG

Cuts at:      0     789    1500 
   Size:        789     711
 
SanDI GG'GwC_CC

Cuts at:      0     122    1500 
   Size:        122    1378
 
SfcI C'TryA_G

Cuts at:      0     204     618    1500 
   Size:        204     414     882

  Fragments arranged by size: 

                 882     414     204
 
SfoI GGC'GCC

Cuts at:      0     270    1500 
   Size:        270    1230
 
SmaI CCC'GGG

Cuts at:      0     214     721    1500 
   Size:        214     507     779

  Fragments arranged by size: 

                 779     507     214
 
SmlI C'TyrA_G

Cuts at:      0     322    1204    1500 
   Size:        322     882     296

  Fragments arranged by size: 

                 882     322     296
 
StyI C'CwwG_G

Cuts at:      0     698    1331    1374    1500 
   Size:        698     633      43     126

  Fragments arranged by size: 

                 698     633     126      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1411    1500 
   Size:       1411      89
 
TatI w'GTAC_w

Cuts at:      0     230    1325    1500 
   Size:        230    1095     175

  Fragments arranged by size: 

                1095     230     175
 
Tth111I GACn'n_nGTC

Cuts at:      0     501    1500 
   Size:        501     999
 
XmaI C'CCGG_G

Cuts at:      0     212     719    1500 
   Size:        212     507     781

  Fragments arranged by size: 

                 781     507     212
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AleI    AlwNI     ApoI
     AseI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstYI     BtgI    BtgZI     BtsI     ClaI
   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
    PflMI     PfoI     PmlI    PpuMI     PsrI    PvuII    SanDI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BciVI     BclI
    BfrBI    BmgBI     BmtI     BsaI    BsaBI     BsgI    BsiWI    BsmBI
    BspHI    BsrBI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    CspCI     DraI     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XhoI     XmnI     ZraI
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