Restriction map for pF1KA0820
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbvCI       BcgI        BfrI        BlnI        BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      Bsu36I      ClaI        
CpoI        DraI        Eam1105I    Eco47III    Eco81I      
EcoRI       EcoT22I     FseI        FspI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MluNI       MroI        MscI        NarI        NdeI        
NotI        NruI        NsiI        PacI        PflFI       
PflMI       PinAI       PmeI        PshAI       PvuI        
RsrII       SacII       SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AclI         2044  AACGTT               AA^CGTT
ApaI         2489  GGGCCC               GGGCC^C
BanI         2229  GGCACC               G^G[CT][GA]CC
BbrPI         382  CACGTG               CAC^GTG
BclI         2036  TGATCA               T^GATCA
BglI          751  GCCATGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2004  AGATCT               A^GATCT
BsiEI        2343  CGGCCG               CG[GA][CT]^CG
BspLU11I     1976  ACATGT               A^CATGT
BsrFI         124  GCCGGC               [GA]^CCGG[CT]
BssSI         492  CACGAG               CACGAG(-5/-1)
Bst1107I     1774  GTATAC               GTA^TAC
BstAPI        232  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2156  TTCGAA               TT^CGAA
BstEII       2273  GGTCACC              G^GT[ACGT]ACC
BstPI        2273  GGTCACC              G^GT[ACGT]ACC
BstXI        1328  CCAAAAAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1774  GTATAC               GTA^TAC
Cfr10I        124  GCCGGC               [GA]^CCGG[CT]
DraIII       2312  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1438  GACATTCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2343  CGGCCG               C^GGCCG
EclXI        2343  CGGCCG               C^GGCCG
Eco52I       2343  CGGCCG               C^GGCCG
EcoO65I      2273  GGTCACC              G^GT[ACGT]ACC
EcoRV         451  GATATC               GAT^ATC
FbaI         2036  TGATCA               T^GATCA
HgaI           52  GACGC                GACGC(5/10)
HincII       1825  GTCGAC               GT[CT]^[GA]AC
HindII       1825  GTCGAC               GT[CT]^[GA]AC
HindIII      1632  AAGCTT               A^AGCTT
MfeI         1930  CAATTG               C^AATTG
MluI           53  ACGCGT               A^CGCGT
MslI         1387  CACATTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1930  CAATTG               C^AATTG
NaeI          124  GCCGGC               GCC^GGC
NcoI         1919  CCATGG               C^CATGG
NgoMIV        124  GCCGGC               G^CCGGC
NheI          564  GCTAGC               G^CTAGC
NspV         2156  TTCGAA               TT^CGAA
PmaCI         382  CACGTG               CAC^GTG
PmlI          382  CACGTG               CAC^GTG
PpuMI         836  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I     2044  AACGTT               AA^CGTT
PvuII        1830  CAGCTG               CAG^CTG
SalI         1825  GTCGAC               G^TCGAC
SexAI        1418  ACCAGGT              A^CC[AT]GGT
SfuI         2156  TTCGAA               TT^CGAA
StuI         2479  AGGCCT               AGG^CCT
XbaI          657  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
              319  CGCG                
AfaI         1325  GTAC                 GT^AC
             1674  GTAC                
AlwNI        1350  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2354  CAGCTCCTG           
AseI          358  ATTAAT               AT^TAAT
             1302  ATTAAT              
AsnI          358  ATTAAT               AT^TAAT
             1302  ATTAAT              
Asp700I       768  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1102  GAACGCTTTC          
AvaI          142  CTCGAG               C^[CT]CG[GA]G
              173  CTCGAG              
BsaAI         382  CACGTG               [CT]AC^GT[GA]
              691  TACGTG              
BsaI         1948  GAGACC               GGTCTC(1/5)
             2514  GAGACC              
BsoBI         142  CTCGAG               C^[CT]CG[GA]G
              173  CTCGAG              
BspHI        1098  TCATGA               T^CATGA
             1586  TCATGA              
BspMI         827  ACCTGC               ACCTGC(4/8)
             2270  GCAGGT              
BsrDI         253  CATTGC               GCAATG(2/0)
              983  CATTGC              
BstUI          54  CGCG                 CG^CG
              319  CGCG                
BstYI        1284  GGATCT               [GA]^GATC[CT]
             2004  AGATCT              
DsaI          104  CCGTGG               C^C[GA][CT]GG
             1919  CCATGG              
EcoNI         828  CCTGCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2109  CCTGATGGAGG         
FauI           30  CCCGC                CCCGC(4/6)
             2493  CCCGC               
MflI         1284  GGATCT               [GA]^GATC[CT]
             2004  AGATCT              
MspA1I         31  CCGCTG               C[AC]G^C[GT]G
             1830  CAGCTG              
MvnI           54  CGCG                 CG^CG
              319  CGCG                
PaeR7I        142  CTCGAG               C^TCGAG
              173  CTCGAG              
PshBI         358  ATTAAT               AT^TAAT
             1302  ATTAAT              
RcaI         1098  TCATGA               T^CATGA
             1586  TCATGA              
RsaI         1325  GTAC                 GT^AC
             1674  GTAC                
SacI          132  GAGCTC               GAGCT^C
             2068  GAGCTC              
XhoI          142  CTCGAG               C^TCGAG
              173  CTCGAG              
XhoII        1284  GGATCT               [GA]^GATC[CT]
             2004  AGATCT              
XmnI          768  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1102  GAACGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccI        AciI        AcsI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanII       
BbsI        BbvI        BciVI       BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com