Restriction map for pF1KA0820
 (Linear) (Six-base) MAPSORT of: KIAA0820.seq  Check: 8026  from: 1  to: 2577



KIAA0820.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     836    2577 
   Size:        836    1741
 
AccI GT'mk_AC

Cuts at:      0    1775    1826    1967    2577 
   Size:       1775      51     141     610

  Fragments arranged by size: 

                1775     610     141      51
 
AclI AA'CG_TT

Cuts at:      0    2045    2577 
   Size:       2045     532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     870    2075    2577 
   Size:        580     290    1205     502

  Fragments arranged by size: 

                1205     580     502     290
 
AflIII A'CryG_T

Cuts at:      0      53     381     383    1976    2577 
   Size:         53     328       2    1593     601

  Fragments arranged by size: 

                1593     601     328      53       2
 
AleI CACnn'nnGTG

Cuts at:      0    1391    2577 
   Size:       1391    1186
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1760    2577 
   Size:       1760     817
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1355    2359    2577 
   Size:       1355    1004     218

  Fragments arranged by size: 

                1355    1004     218
 
ApaI G_GGCC'C

Cuts at:      0    2493    2577 
   Size:       2493      84
 
ApoI r'AATT_y

Cuts at:      0     268     945    2061    2577 
   Size:        268     677    1116     516

  Fragments arranged by size: 

                1116     677     516     268
 
AseI AT'TA_AT

Cuts at:      0     359    1303    2577 
   Size:        359     944    1274

  Fragments arranged by size: 

                1274     944     359
 
AvaI C'yCGr_G

Cuts at:      0     142     173    2577 
   Size:        142      31    2404

  Fragments arranged by size: 

                2404     142      31
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1980    2013    2577 
   Size:       1980      33     564

  Fragments arranged by size: 

                1980     564      33
 
BanI G'GyrC_C

Cuts at:      0    2229    2577 
   Size:       2229     348
 
BanII G_rGCy'C

Cuts at:      0     136    2072    2394    2460    2493    2577 
   Size:        136    1936     322      66      33      84

  Fragments arranged by size: 

                1936     322     136      84      66      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     965     979    1414    1478    2577 
   Size:        965      14     435      64    1099

  Fragments arranged by size: 

                1099     965     435      64      14
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     864    1600    2008    2249    2577 
   Size:        864     736     408     241     328

  Fragments arranged by size: 

                 864     736     408     328     241
 
BclI T'GATC_A

Cuts at:      0    2036    2577 
   Size:       2036     541
 
BglI GCCn_nnn'nGGC

Cuts at:      0     757    2577 
   Size:        757    1820
 
BglII A'GATC_T

Cuts at:      0    2004    2577 
   Size:       2004     573
 
BlpI GC'TnA_GC

Cuts at:      0    1496    2126    2135    2577 
   Size:       1496     630       9     442

  Fragments arranged by size: 

                1496     630     442       9
 
Bme1580I G_kGCm'C

Cuts at:      0    2493    2577 
   Size:       2493      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2492    2577 
   Size:       2492      85
 
BmtI G_CTAG'C

Cuts at:      0     568    2577 
   Size:        568    2009
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     509    1196    2222    2381    2577 
   Size:        106     403     687    1026     159     196

  Fragments arranged by size: 

                1026     687     403     196     159     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     569    1816    2577 
   Size:        316     253    1247     761

  Fragments arranged by size: 

                1247     761     316     253
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1942    2508    2577 
   Size:       1942     566      69

  Fragments arranged by size: 

                1942     566      69
 
BsaAI yAC'GTr

Cuts at:      0     384     693    2577 
   Size:        384     309    1884

  Fragments arranged by size: 

                1884     384     309
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34    1518    2270    2384    2577 
   Size:         34    1484     752     114     193

  Fragments arranged by size: 

                1484     752     193     114      34
 
BseYI C'CCAG_C

Cuts at:      0      66    1855    2434    2577 
   Size:         66    1789     579     143

  Fragments arranged by size: 

                1789     579     143      66
 
BsiEI CG_ry'CG

Cuts at:      0    2346    2577 
   Size:       2346     231
 
BsiHKAI G_wGCw'C

Cuts at:      0     136     143    2072    2322    2577 
   Size:        136       7    1929     250     255

  Fragments arranged by size: 

                1929     255     250     136       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     143    2072    2322    2394    2460    2493    2577 
   Size:        136       7    1929     250      72      66      33      84

  Fragments arranged by size: 

                1929     250     136      84      72      66      33       7
            
 
BspHI T'CATG_A

Cuts at:      0    1098    1586    2577 
   Size:       1098     488     991

  Fragments arranged by size: 

                1098     991     488
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     836    2261    2577 
   Size:        836    1425     316

  Fragments arranged by size: 

                1425     836     316
 
BsrDI GCAATG_nn'

Cuts at:      0     252     982    2577 
   Size:        252     730    1595

  Fragments arranged by size: 

                1595     730     252
 
BsrFI r'CCGG_y

Cuts at:      0     124    2577 
   Size:        124    2453
 
BssSI C'ACGA_G

Cuts at:      0     492    2577 
   Size:        492    2085
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     238    2577 
   Size:        238    2339
 
BstBI TT'CG_AA

Cuts at:      0    2157    2577 
   Size:       2157     420
 
BstEII G'GTnAC_C

Cuts at:      0    2273    2577 
   Size:       2273     304
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1335    2577 
   Size:       1335    1242
 
BstYI r'GATC_y

Cuts at:      0    1284    2004    2577 
   Size:       1284     720     573

  Fragments arranged by size: 

                1284     720     573
 
BstZ17I GTA'TAC

Cuts at:      0    1776    2577 
   Size:       1776     801
 
BtgI C'CryG_G

Cuts at:      0     104    1919    2577 
   Size:        104    1815     658

  Fragments arranged by size: 

                1815     658     104
 
BtsI GCAGTG_nn'

Cuts at:      0    2448    2577 
   Size:       2448     129
 
DraIII CAC_nnn'GTG

Cuts at:      0    2317    2577 
   Size:       2317     260
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1444    2577 
   Size:       1444    1133
 
EaeI y'GGCC_r

Cuts at:      0     114    2210    2343    2577 
   Size:        114    2096     133     234

  Fragments arranged by size: 

                2096     234     133     114
 
EagI C'GGCC_G

Cuts at:      0    2343    2577 
   Size:       2343     234
 
EarI CTCTTCn'nnn_

Cuts at:      0     683    1018    1149    1270    2577 
   Size:        683     335     131     121    1307

  Fragments arranged by size: 

                1307     683     335     131     121
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2539    2577 
   Size:       2539      38
 
EcoICRI GAG'CTC

Cuts at:      0     134    2070    2577 
   Size:        134    1936     507

  Fragments arranged by size: 

                1936     507     134
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     509     580     870    1196    2075    2222    2381 
   Size:        106     403      71     290     326     879     147     159

Cuts at:   2381    2577 
   Size:        196

  Fragments arranged by size: 

                 879     403     326     290     196     159     147     106
                  71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     832    2113    2577 
   Size:        832    1281     464

  Fragments arranged by size: 

                1281     832     464
 
EcoO109I rG'GnC_Cy

Cuts at:      0     837    1264    2489    2490    2577 
   Size:        837     427    1225       1      87

  Fragments arranged by size: 

                1225     837     427      87       1
 
EcoRV GAT'ATC

Cuts at:      0     453    2577 
   Size:        453    2124
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     310    1758    2200    2577 
   Size:        310    1448     442     377

  Fragments arranged by size: 

                1448     442     377     310
 
HaeII r_GCGC'y

Cuts at:      0      67     125    2142    2577 
   Size:         67      58    2017     435

  Fragments arranged by size: 

                2017     435      67      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     896     928    1269    1301    1494    1526    1844    1876 
   Size:        896      32     341      32     193      32     318      32

Cuts at:   1876    2577 
   Size:        701

  Fragments arranged by size: 

                 896     701     341     318     193      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1827    2577 
   Size:       1827     750
 
HindIII A'AGCT_T

Cuts at:      0    1632    2577 
   Size:       1632     945
 
MfeI C'AATT_G

Cuts at:      0    1930    2577 
   Size:       1930     647
 
MluI A'CGCG_T

Cuts at:      0      53    2577 
   Size:         53    2524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1515    2577 
   Size:       1515    1062
 
MslI CAynn'nnrTG

Cuts at:      0    1391    2577 
   Size:       1391    1186
 
MspA1I CmG'CkG

Cuts at:      0      33    1832    2577 
   Size:         33    1799     745

  Fragments arranged by size: 

                1799     745      33
 
NaeI GCC'GGC

Cuts at:      0     126    2577 
   Size:        126    2451
 
NcoI C'CATG_G

Cuts at:      0    1919    2577 
   Size:       1919     658
 
NgoMIV G'CCGG_C

Cuts at:      0     124    2577 
   Size:        124    2453
 
NheI G'CTAG_C

Cuts at:      0     564    2577 
   Size:        564    2013
 
NspI r_CATG'y

Cuts at:      0    1680    1808    1980    2577 
   Size:       1680     128     172     597

  Fragments arranged by size: 

                1680     597     172     128
 
PciI A'CATG_T

Cuts at:      0    1976    2577 
   Size:       1976     601
 
PfoI T'CCnGG_A

Cuts at:      0    2251    2577 
   Size:       2251     326
 
PmlI CAC'GTG

Cuts at:      0     384    2577 
   Size:        384    2193
 
PpuMI rG'GwC_Cy

Cuts at:      0     837    2577 
   Size:        837    1740
 
PspOMI G'GGCC_C

Cuts at:      0    2489    2577 
   Size:       2489      88
 
PspXI vC'TCGA_Gb

Cuts at:      0     173    2577 
   Size:        173    2404
 
PstI C_TGCA'G

Cuts at:      0      50     215     833     992    2577 
   Size:         50     165     618     159    1585

  Fragments arranged by size: 

                1585     618     165     159      50
 
PvuII CAG'CTG

Cuts at:      0    1832    2577 
   Size:       1832     745
 
SacI G_AGCT'C

Cuts at:      0     136    2072    2577 
   Size:        136    1936     505

  Fragments arranged by size: 

                1936     505     136
 
SalI G'TCGA_C

Cuts at:      0    1825    2577 
   Size:       1825     752
 
SexAI A'CCwGG_T

Cuts at:      0    1418    2577 
   Size:       1418    1159
 
SfcI C'TryA_G

Cuts at:      0      46     211     829     898     988    1877    2577 
   Size:         46     165     618      69      90     889     700

  Fragments arranged by size: 

                 889     700     618     165      90      69      46
 
SmlI C'TyrA_G

Cuts at:      0     142     173     295     584    1795    2577 
   Size:        142      31     122     289    1211     782

  Fragments arranged by size: 

                1211     782     289     142     122      31
 
StuI AGG'CCT

Cuts at:      0    2481    2577 
   Size:       2481      96
 
StyI C'CwwG_G

Cuts at:      0    1685    1919    2184    2577 
   Size:       1685     234     265     393

  Fragments arranged by size: 

                1685     393     265     234
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     824    2577 
   Size:        824    1753
 
TatI w'GTAC_w

Cuts at:      0    1673    2577 
   Size:       1673     904
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1529    1561    2577 
   Size:       1529      32    1016

  Fragments arranged by size: 

                1529    1016      32
 
XbaI T'CTAG_A

Cuts at:      0     657    2577 
   Size:        657    1920
 
XhoI C'TCGA_G

Cuts at:      0     142     173    2577 
   Size:        142      31    2404

  Fragments arranged by size: 

                2404     142      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     772    1106    2577 
   Size:        772     334    1471

  Fragments arranged by size: 

                1471     772     334
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI
     PciI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI     SalI    SexAI     SfcI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AloI    ApaLI
     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI    BfrBI
    BmgBI     BplI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII   Bsu36I
    BtgZI     ClaI    CspCI     DraI    EcoRI     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MscI     NarI     NdeI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XcmI     XmaI     ZraI
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