Restriction map for pF1KA0804
 (Linear) (Six-base) MAPSORT of: KIAA0804.seq  Check: 1779  from: 1  to: 4278



KIAA0804.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0    1331    2048    2676    4278 
   Size:       1331     717     628    1602

  Fragments arranged by size: 

                1602    1331     717     628
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2246    2926    2962    3512    3520    4278 
   Size:       2246     680      36     550       8     758

  Fragments arranged by size: 

                2246     758     680     550      36       8
 
AfeI AGC'GCT

Cuts at:      0    1955    4278 
   Size:       1955    2323
 
AflIII A'CryG_T

Cuts at:      0    1164    4278 
   Size:       1164    3114
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2623    4278 
   Size:       2623    1655
 
AleI CACnn'nnGTG

Cuts at:      0    1237    4278 
   Size:       1237    3041
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1255    4278 
   Size:       1255    3023
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153    1106    1138    2642    2674    4278 
   Size:        121      32     953      32    1504      32    1604

  Fragments arranged by size: 

                1604    1504     953     121      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1386    3459    4063    4278 
   Size:       1386    2073     604     215

  Fragments arranged by size: 

                2073    1386     604     215
 
ApoI r'AATT_y

Cuts at:      0     103     419    1224    1815    2068    2263    2353    2460 
   Size:        103     316     805     591     253     195      90     107

Cuts at:   2460    2575    2714    2722    3001    3381    3883    3954    4278 
   Size:        115     139       8     279     380     502      71     324

  Fragments arranged by size: 

                 805     591     502     380     324     316     279     253
                 195     139     115     107     103      90      71       8
            
 
AseI AT'TA_AT

Cuts at:      0    1936    4278 
   Size:       1936    2342
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3338    3371    4278 
   Size:       3338      33     907

  Fragments arranged by size: 

                3338     907      33
 
BamHI G'GATC_C

Cuts at:      0     255    1032    3321    3579    4032    4071    4278 
   Size:        255     777    2289     258     453      39     207

  Fragments arranged by size: 

                2289     777     453     258     255     207      39
 
BanI G'GyrC_C

Cuts at:      0    1734    3244    4278 
   Size:       1734    1510    1034

  Fragments arranged by size: 

                1734    1510    1034
 
BanII G_rGCy'C

Cuts at:      0    1674    4278 
   Size:       1674    2604
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3007    4278 
   Size:       3007    1271
 
BbvCI CC'TCA_GC

Cuts at:      0    1483    4278 
   Size:       1483    2795
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     495    4278 
   Size:        495    3783
 
BclI T'GATC_A

Cuts at:      0     534    2562    4095    4278 
   Size:        534    2028    1533     183

  Fragments arranged by size: 

                2028    1533     534     183
 
BglII A'GATC_T

Cuts at:      0    1310    1442    2229    3696    4278 
   Size:       1310     132     787    1467     582

  Fragments arranged by size: 

                1467    1310     787     582     132
 
Bme1580I G_kGCm'C

Cuts at:      0    1687    4278 
   Size:       1687    2591
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3342    4278 
   Size:       3342     936
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     704    1175    1385    1512    2669    4110    4278 
   Size:        154     550     471     210     127    1157    1441     168

  Fragments arranged by size: 

                1441    1157     550     471     210     168     154     127
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1483    4278 
   Size:       1483    2795
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     271     612    1870    3166    4278 
   Size:        164     107     341    1258    1296    1112

  Fragments arranged by size: 

                1296    1258    1112     341     164     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3352    4278 
   Size:       3352     926
 
BsaAI yAC'GTr

Cuts at:      0    2776    4278 
   Size:       2776    1502
 
BsaBI GATnn'nnATC

Cuts at:      0     539     713    4278 
   Size:        539     174    3565

  Fragments arranged by size: 

                3565     539     174
 
BsaHI Gr'CG_yC

Cuts at:      0    3795    4278 
   Size:       3795     483
 
BsaWI w'CCGG_w

Cuts at:      0    1634    2684    4278 
   Size:       1634    1050    1594

  Fragments arranged by size: 

                1634    1594    1050
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     779     809    1106    1136    1358    1388    2584    2614 
   Size:        779      30     297      30     222      30    1196      30

Cuts at:   2614    2642    2672    2842    2872    4278 
   Size:         28      30     170      30    1406

  Fragments arranged by size: 

                1406    1196     779     297     222     170      30      30
                  30      30      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     182    1127    1500    2099    2102    2863    3024    4129 
   Size:        182     945     373     599       3     761     161    1105

Cuts at:   4129    4278 
   Size:        149

  Fragments arranged by size: 

                1105     945     761     599     373     182     161     149
                   3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2864    4278 
   Size:       2864    1414
 
BsiHKAI G_wGCw'C

Cuts at:      0    1244    1674    4206    4278 
   Size:       1244     430    2532      72

  Fragments arranged by size: 

                2532    1244     430      72
 
BsmI GAATG_Cn'

Cuts at:      0     897    2124    2280    3877    4278 
   Size:        897    1227     156    1597     401

  Fragments arranged by size: 

                1597    1227     897     401     156
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3786    4114    4278 
   Size:       3786     328     164

  Fragments arranged by size: 

                3786     328     164
 
Bsp1286I G_dGCh'C

Cuts at:      0    1244    1674    1687    4206    4278 
   Size:       1244     430      13    2519      72

  Fragments arranged by size: 

                2519    1244     430      72      13
 
BspEI T'CCGG_A

Cuts at:      0    2684    4278 
   Size:       2684    1594
 
BspHI T'CATG_A

Cuts at:      0    3198    4278 
   Size:       3198    1080
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1986    2185    2613    4278 
   Size:       1986     199     428    1665

  Fragments arranged by size: 

                1986    1665     428     199
 
BsrDI GCAATG_nn'

Cuts at:      0    2102    2882    3362    3446    4278 
   Size:       2102     780     480      84     832

  Fragments arranged by size: 

                2102     832     780     480      84
 
BsrGI T'GTAC_A

Cuts at:      0    1962    4278 
   Size:       1962    2316
 
BssSI C'ACGA_G

Cuts at:      0    2896    4278 
   Size:       2896    1382
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2200    3544    4278 
   Size:       2200    1344     734

  Fragments arranged by size: 

                2200    1344     734
 
BstEII G'GTnAC_C

Cuts at:      0    3732    4278 
   Size:       3732     546
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2947    4278 
   Size:       2947    1331
 
BstYI r'GATC_y

Cuts at:      0     255    1032    1310    1442    1776    2229    3321    3579 
   Size:        255     777     278     132     334     453    1092     258

Cuts at:   3579    3696    4032    4071    4278 
   Size:        117     336      39     207

  Fragments arranged by size: 

                1092     777     453     336     334     278     258     255
                 207     132     117      39
 
Bsu36I CC'TnA_GG

Cuts at:      0    2300    4278 
   Size:       2300    1978
 
BtgI C'CryG_G

Cuts at:      0     950    4278 
   Size:        950    3328
 
BtsI GCAGTG_nn'

Cuts at:      0     506    1048    4037    4278 
   Size:        506     542    2989     241

  Fragments arranged by size: 

                2989     542     506     241
 
DraI TTT'AAA

Cuts at:      0    2391    3524    4278 
   Size:       2391    1133     754

  Fragments arranged by size: 

                2391    1133     754
 
DraIII CAC_nnn'GTG

Cuts at:      0     668    4278 
   Size:        668    3610
 
EaeI y'GGCC_r

Cuts at:      0     677     947    1023    1823    3834    4278 
   Size:        677     270      76     800    2011     444

  Fragments arranged by size: 

                2011     800     677     444     270      76
 
EarI CTCTTCn'nnn_

Cuts at:      0      56    2663    4278 
   Size:         56    2607    1615

  Fragments arranged by size: 

                2607    1615      56
 
EcoICRI GAG'CTC

Cuts at:      0    1672    4278 
   Size:       1672    2606
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     704    1175    1385    1512    2246    2669    2926 
   Size:        154     550     471     210     127     734     423     257

Cuts at:   2926    2962    3512    3520    4110    4278 
   Size:         36     550       8     590     168

  Fragments arranged by size: 

                 734     590     550     550     471     423     257     210
                 168     154     127      36       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2568    2625    3414    4278 
   Size:       2568      57     789     864

  Fragments arranged by size: 

                2568     864     789      57
 
EcoRI G'AATT_C

Cuts at:      0    1815    2460    2575    4278 
   Size:       1815     645     115    1703

  Fragments arranged by size: 

                1815    1703     645     115
 
EcoRV GAT'ATC

Cuts at:      0    1974    4278 
   Size:       1974    2304
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     112    1546    3619    4278 
   Size:        112    1434    2073     659

  Fragments arranged by size: 

                2073    1434     659     112
 
HaeII r_GCGC'y

Cuts at:      0     596    1957    4278 
   Size:        596    1361    2321

  Fragments arranged by size: 

                2321    1361     596
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     292     309     324    1491    1523    2584    2609 
   Size:        277      15      17      15    1167      32    1061      25

Cuts at:   2609    2616    2641    2842    2874    3351    3383    3435    3467 
   Size:          7      25     201      32     477      32      52      32

Cuts at:   3467    3716    3748    3751    3783    4278 
   Size:        249      32       3      32     495

  Fragments arranged by size: 

                1167    1061     495     477     277     249     201      52
                  32      32      32      32      32      32      25      25
                  17      15      15       7       3
 
HincII GTy'rAC

Cuts at:      0    2677    4278 
   Size:       2677    1601
 
HindIII A'AGCT_T

Cuts at:      0      89     545    4278 
   Size:         89     456    3733

  Fragments arranged by size: 

                3733     456      89
 
MfeI C'AATT_G

Cuts at:      0     476    4278 
   Size:        476    3802
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     558     784    1377    4278 
   Size:        220     338     226     593    2901

  Fragments arranged by size: 

                2901     593     338     226     220
 
MscI TGG'CCA

Cuts at:      0     679     949    1825    3836    4278 
   Size:        679     270     876    2011     442

  Fragments arranged by size: 

                2011     876     679     442     270
 
MslI CAynn'nnrTG

Cuts at:      0      23     118     296    1237    2686    2747    3302    4278 
   Size:         23      95     178     941    1449      61     555     976

  Fragments arranged by size: 

                1449     976     941     555     178      95      61      23
            
 
MspA1I CmG'CkG

Cuts at:      0     445     785    1326    4245    4278 
   Size:        445     340     541    2919      33

  Fragments arranged by size: 

                2919     541     445     340      33
 
NcoI C'CATG_G

Cuts at:      0     950    4278 
   Size:        950    3328
 
NdeI CA'TA_TG

Cuts at:      0    1967    4278 
   Size:       1967    2311
 
NspI r_CATG'y

Cuts at:      0    1168    1270    3911    4278 
   Size:       1168     102    2641     367

  Fragments arranged by size: 

                2641    1168     367     102
 
PciI A'CATG_T

Cuts at:      0    1164    4278 
   Size:       1164    3114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1386    1710    3589    3804    4189    4278 
   Size:       1386     324    1879     215     385      89

  Fragments arranged by size: 

                1879    1386     385     324     215      89
 
PfoI T'CCnGG_A

Cuts at:      0     720    2832    4278 
   Size:        720    2112    1446

  Fragments arranged by size: 

                2112    1446     720
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      23      55    1106    1138    2642    2674    4278 
   Size:         23      32    1051      32    1504      32    1604

  Fragments arranged by size: 

                1604    1504    1051      32      32      32      23
 
PpuMI rG'GwC_Cy

Cuts at:      0    2568    2625    4278 
   Size:       2568      57    1653

  Fragments arranged by size: 

                2568    1653      57
 
PstI C_TGCA'G

Cuts at:      0     444    1111    1769    2645    4250    4278 
   Size:        444     667     658     876    1605      28

  Fragments arranged by size: 

                1605     876     667     658     444      28
 
PvuII CAG'CTG

Cuts at:      0     445    1326    4245    4278 
   Size:        445     881    2919      33

  Fragments arranged by size: 

                2919     881     445      33
 
SacI G_AGCT'C

Cuts at:      0    1674    4278 
   Size:       1674    2604
 
SalI G'TCGA_C

Cuts at:      0    2675    4278 
   Size:       2675    1603
 
SapI GCTCTTCn'nnn_

Cuts at:      0      56    4278 
   Size:         56    4222
 
SexAI A'CCwGG_T

Cuts at:      0    2252    2984    4278 
   Size:       2252     732    1294

  Fragments arranged by size: 

                2252    1294     732
 
SfcI C'TryA_G

Cuts at:      0     440     470    1107    1765    2641    4176    4246    4278 
   Size:        440      30     637     658     876    1535      70      32

  Fragments arranged by size: 

                1535     876     658     637     440      70      32      30
            
 
SmlI C'TyrA_G

Cuts at:      0     179     250     627    1849    3145    4278 
   Size:        179      71     377    1222    1296    1133

  Fragments arranged by size: 

                1296    1222    1133     377     179      71
 
SpeI A'CTAG_T

Cuts at:      0     571    4278 
   Size:        571    3707
 
SspI AAT'ATT

Cuts at:      0     530     734    4278 
   Size:        530     204    3544

  Fragments arranged by size: 

                3544     530     204
 
StyI C'CwwG_G

Cuts at:      0     866     950    1692    2211    2240    3025    4278 
   Size:        866      84     742     519      29     785    1253

  Fragments arranged by size: 

                1253     866     785     742     519      84      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2953    3720    4278 
   Size:       2953     767     558

  Fragments arranged by size: 

                2953     767     558
 
TatI w'GTAC_w

Cuts at:      0    1074    1962    3785    4278 
   Size:       1074     888    1823     493

  Fragments arranged by size: 

                1823    1074     888     493
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4067    4099    4278 
   Size:       4067      32     179

  Fragments arranged by size: 

                4067     179      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     557    4278 
   Size:        557    3721
 
XbaI T'CTAG_A

Cuts at:      0      84     844    4278 
   Size:         84     760    3434

  Fragments arranged by size: 

                3434     760      84
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     672    1055    4278 
   Size:        672     383    3223

  Fragments arranged by size: 

                3223     672     383
 
XmnI GAAnn'nnTTC

Cuts at:      0    2092    3035    3170    4064    4278 
   Size:       2092     943     135     894     214

  Fragments arranged by size: 

                2092     943     894     214     135
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI    BciVI     BclI    BglII Bme1580I     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI     PstI    PvuII     SacI
     SalI     SapI    SexAI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BbeI     BcgI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI    BseYI    BsiEI    BsiWI    BsrBI
    BsrFI   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DrdI
     EagI     EciI    EcoNI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI     XhoI     XmaI     ZraI
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