Restriction map for pF1KA0802
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AflIII      AgeI        AscI        AspI        AvrII       
BbrPI       BciVI       BglII       BlnI        BsaAI       
BsaBI       BsiWI       BspDI       BspHI       BspLU11I    
BsrDI       Bst1107I    BstZ17I     ClaI        CpoI        
DraI        DraIII      EcoRV       EcoT22I     HindIII     
HpaI        MamI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RcaI        
RsrII       ScaI        SexAI       SfiI        SgrAI       
SnaBI       SpeI        SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I       3341  GGTACC               G^GTACC
AccI         3481  GTCGAC               GT^[AC][GT]AC
AccIII        863  TCCGGA               T^CCGGA
AclI         2797  AACGTT               AA^CGTT
AcsI         2713  GAATTC               [GA]^AATT[CT]
AflII         235  CTTAAG               C^TTAAG
AhdI         1723  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1100  AGCGCT               AGC^GCT
ApoI         2713  GAATTC               [GA]^AATT[CT]
AseI         4192  ATTAAT               AT^TAAT
AsnI         4192  ATTAAT               AT^TAAT
Asp718I      3341  GGTACC               G^GTACC
AspEI        1723  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        4472  GGATCC               G^GATCC
BbeI         3638  GGCGCC               GGCGC^C
BbvCI         265  GCTGAGG              CCTCAGC(-5/-2)
BclI         2781  TGATCA               T^GATCA
BfrI          235  CTTAAG               C^TTAAG
BseAI         863  TCCGGA               T^CCGGA
Bsp1407I      389  TGTACA               T^GTACA
BspEI         863  TCCGGA               T^CCGGA
BsrGI         389  TGTACA               T^GTACA
BssHII        255  GCGCGC               G^CGCGC
BstBI         173  TTCGAA               TT^CGAA
BstEII       2170  GGTCACC              G^GT[ACGT]ACC
BstPI        2170  GGTCACC              G^GT[ACGT]ACC
EagI         2893  CGGCCG               C^GGCCG
Eam1105I     1723  GACTTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2893  CGGCCG               C^GGCCG
Eco47III     1100  AGCGCT               AGC^GCT
Eco52I       2893  CGGCCG               C^GGCCG
EcoO65I      2170  GGTCACC              G^GT[ACGT]ACC
EcoRI        2713  GAATTC               G^AATTC
FbaI         2781  TGATCA               T^GATCA
FseI         1766  GGCCGGCC             GGCCGG^CC
HgaI         3099  GCGTC                GACGC(5/10)
HincII       3481  GTCGAC               GT[CT]^[GA]AC
HindII       3481  GTCGAC               GT[CT]^[GA]AC
KasI         3638  GGCGCC               G^GCGCC
KpnI         3341  GGTACC               GGTAC^C
KspI         2285  CCGCGG               CCGC^GG
MroI          863  TCCGGA               T^CCGGA
NarI         3638  GGCGCC               GG^CGCC
NcoI         4592  CCATGG               C^CATGG
NspV          173  TTCGAA               TT^CGAA
PshBI        4192  ATTAAT               AT^TAAT
Psp1406I     2797  AACGTT               AA^CGTT
SacII        2285  CCGCGG               CCGC^GG
SalI         3481  GTCGAC               G^TCGAC
SapI         2980  GAAGAGC              GCTCTTC(1/4)
SbfI          999  CCTGCAGG             CCTGCA^GG
SfoI         3638  GGCGCC               GGC^GCC
SfuI          173  TTCGAA               TT^CGAA
SphI         2831  GCATGC               GCATG^C
Sse8387I      999  CCTGCAGG             CCTGCA^GG
SspBI         389  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AatII        1272  GACGTC               GACGT^C
             4104  GACGTC              
Alw44I       2563  GTGCAC               G^TGCAC
             4180  GTGCAC              
AocI         3028  CCTGAGG              CC^T[ACGT]AGG
             3969  CCTTAGG             
ApaLI        2563  GTGCAC               G^TGCAC
             4180  GTGCAC              
AviII         476  TGCGCA               TGC^GCA
             3473  TGCGCA              
BalI          488  TGGCCA               TGG^CCA
             2846  TGGCCA              
BcgI         1161  CGAGAGTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               48  GCAGGAACTTCG        
BsaWI         863  TCCGGA               [AT]^CCGG[AT]
             2009  TCCGGT              
BsiEI        2667  CGACCG               CG[GA][CT]^CG
             2893  CGGCCG              
BsmBI        3953  CGTCTC               CGTCTC(1/5)
              145  GAGACG              
BsmI         3753  GAATGC               GAATGC(1/-1)
              773  GCATTC              
BstAPI       3405  GCAGCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4025  GCACATGCTGC         
BstYI        2618  AGATCC               [GA]^GATC[CT]
             4472  GGATCC              
Bsu36I       3028  CCTGAGG              CC^T[ACGT]AGG
             3969  CCTTAGG             
DrdI         1668  GACTTCCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3807  GACCAATGGGTC        
Eco81I       3028  CCTGAGG              CC^T[ACGT]AGG
             3969  CCTTAGG             
EcoT14I      3554  CCAAGG               C^C[AT][AT]GG
             4592  CCATGG              
FspI          476  TGCGCA               TGC^GCA
             3473  TGCGCA              
MflI         2618  AGATCC               [GA]^GATC[CT]
             4472  GGATCC              
MluNI         488  TGGCCA               TGG^CCA
             2846  TGGCCA              
MscI          488  TGGCCA               TGG^CCA
             2846  TGGCCA              
NaeI         1767  GCCGGC               GCC^GGC
             4273  GCCGGC              
NgoMIV       1767  GCCGGC               G^CCGGC
             4273  GCCGGC              
StuI         1580  AGGCCT               AGG^CCT
             2348  AGGCCT              
StyI         3554  CCAAGG               C^C[AT][AT]GG
             4592  CCATGG              
Tsp509I      2714  AATT                 ^AATT
             4339  AATT                
XcmI         1691  CCATCGGGAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2842  CCAGTGGCCATGTGG     
XhoII        2618  AGATCC               [GA]^GATC[CT]
             4472  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XmaCI       
XmaI        XmnI        
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