Restriction map for pF1KA0802
 (Linear) (Six-base) MAPSORT of: KIAA0802.seq  Check: 8784  from: 1  to: 4635



KIAA0802.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     839    1584    3490    3575    4635 
   Size:        839     745    1906      85    1060

  Fragments arranged by size: 

                1906    1060     839     745      85
 
AatII G_ACGT'C

Cuts at:      0    1276    4108    4635 
   Size:       1276    2832     527

  Fragments arranged by size: 

                2832    1276     527
 
AccI GT'mk_AC

Cuts at:      0    3482    4635 
   Size:       3482    1153
 
Acc65I G'GTAC_C

Cuts at:      0    3341    4635 
   Size:       3341    1294
 
AclI AA'CG_TT

Cuts at:      0    2798    4635 
   Size:       2798    1837
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1033    1354    1860    2929    3890    4635 
   Size:        640     393     321     506    1069     961     745

  Fragments arranged by size: 

                1069     961     745     640     506     393     321
 
AfeI AGC'GCT

Cuts at:      0    1102    4635 
   Size:       1102    3533
 
AflII C'TTAA_G

Cuts at:      0     235    4635 
   Size:        235    4400
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1728    4635 
   Size:       1728    2907
 
AleI CACnn'nnGTG

Cuts at:      0    2198    3251    4414    4635 
   Size:       2198    1053    1163     221

  Fragments arranged by size: 

                2198    1163    1053     221
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1922    2435    4635 
   Size:       1922     513    2200

  Fragments arranged by size: 

                2200    1922     513
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1533    2331    2869    3411    4635 
   Size:       1533     798     538     542    1224

  Fragments arranged by size: 

                1533    1224     798     542     538
 
ApaI G_GGCC'C

Cuts at:      0     596     751     996    1720    4152    4318    4538    4592 
   Size:        596     155     245     724    2432     166     220      54

Cuts at:   4592    4635 
   Size:         43

  Fragments arranged by size: 

                2432     724     596     245     220     166     155      54
                  43
 
ApaLI G'TGCA_C

Cuts at:      0    2563    4180    4635 
   Size:       2563    1617     455

  Fragments arranged by size: 

                2563    1617     455
 
ApoI r'AATT_y

Cuts at:      0    2713    4635 
   Size:       2713    1922
 
AseI AT'TA_AT

Cuts at:      0    4193    4635 
   Size:       4193     442
 
AvaI C'yCGr_G

Cuts at:      0     380     605     750     941    1145    1340    1679    1762 
   Size:        380     225     145     191     204     195     339      83

Cuts at:   1762    1772    2146    2246    3001    3543    3614    4343    4539 
   Size:         10     374     100     755     542      71     729     196

Cuts at:   4539    4635 
   Size:         96

  Fragments arranged by size: 

                 755     729     542     380     374     339     225     204
                 196     195     191     145     100      96      83      71
                  10
 
BamHI G'GATC_C

Cuts at:      0    4472    4635 
   Size:       4472     163
 
BanI G'GyrC_C

Cuts at:      0     437     979    3341    3638    4333    4635 
   Size:        437     542    2362     297     695     302

  Fragments arranged by size: 

                2362     695     542     437     302     297
 
BanII G_rGCy'C

Cuts at:      0     530     596     751     821     917     996    1043    1331 
   Size:        530      66     155      70      96      79      47     288

Cuts at:   1331    1526    1720    2009    3544    4152    4177    4301    4318 
   Size:        195     194     289    1535     608      25     124      17

Cuts at:   4318    4538    4592    4635 
   Size:        220      54      43

  Fragments arranged by size: 

                1535     608     530     289     288     220     195     194
                 155     124      96      79      70      66      54      47
                  43      25      17
 
BbeI G_GCGC'C

Cuts at:      0    3642    4635 
   Size:       3642     993
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     893    1276    2497    2894    4635 
   Size:        100     793     383    1221     397    1741

  Fragments arranged by size: 

                1741    1221     793     397     383     100
 
BbvCI CC'TCA_GC

Cuts at:      0     266    4635 
   Size:        266    4369
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      37      71    1150    1184    4635 
   Size:         37      34    1079      34    3451

  Fragments arranged by size: 

                3451    1079      37      34      34
 
BclI T'GATC_A

Cuts at:      0    2781    4635 
   Size:       2781    1854
 
BglI GCCn_nnn'nGGC

Cuts at:      0     954    1487    2347    4635 
   Size:        954     533     860    2288

  Fragments arranged by size: 

                2288     954     860     533
 
BlpI GC'TnA_GC

Cuts at:      0     916    1042    3580    4290    4635 
   Size:        916     126    2538     710     345

  Fragments arranged by size: 

                2538     916     710     345     126
 
Bme1580I G_kGCm'C

Cuts at:      0     596     751     996    1137    1720    2397    2567    3305 
   Size:        596     155     245     141     583     677     170     738

Cuts at:   3305    3636    4152    4184    4318    4411    4538    4592    4635 
   Size:        331     516      32     134      93     127      54      43

  Fragments arranged by size: 

                 738     677     596     583     516     331     245     170
                 155     141     134     127      93      54      43      32
            
 
BmgBI CAC'GTC

Cuts at:      0    2445    3423    4635 
   Size:       2445     978    1212

  Fragments arranged by size: 

                2445    1212     978
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1133    4635 
   Size:       1133    3502
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1266    2154    4635 
   Size:       1266     888    2481

  Fragments arranged by size: 

                2481    1266     888
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     313     361     430     514     538     703     823 
   Size:        202     111      48      69      84      24     165     120

Cuts at:    823    1468    1609    1771    1903    1995    2124    2377    3167 
   Size:        645     141     162     132      92     129     253     790

Cuts at:   3167    3551    3718    4191    4635 
   Size:        384     167     473     444

  Fragments arranged by size: 

                 790     645     473     444     384     253     202     167
                 165     162     141     132     129     120     111      92
                  84      69      48      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     266    4635 
   Size:        266    4369
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688    1932    2446    3178    3735    4635 
   Size:        688    1244     514     732     557     900

  Fragments arranged by size: 

                1244     900     732     688     557     514
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2125    4289    4549    4635 
   Size:       2125    2164     260      86

  Fragments arranged by size: 

                2164    2125     260      86
 
BsaHI Gr'CG_yC

Cuts at:      0    1273    3639    4105    4635 
   Size:       1273    2366     466     530

  Fragments arranged by size: 

                2366    1273     530     466
 
BsaWI w'CCGG_w

Cuts at:      0     863    2009    4635 
   Size:        863    1146    2626

  Fragments arranged by size: 

                2626    1146     863
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     311     341    1000    1030    1246    1276    1996    2026 
   Size:        311      30     659      30     216      30     720      30

Cuts at:   2026    4635 
   Size:       2609

  Fragments arranged by size: 

                2609     720     659     311     216      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     658    1021    1210    1270    1510    1906    1909    2107 
   Size:        658     363     189      60     240     396       3     198

Cuts at:   2107    2119    2511    3220    3324    4525    4530    4635 
   Size:         12     392     709     104    1201       5     105

  Fragments arranged by size: 

                1201     709     658     396     392     363     240     198
                 189     105     104      60      12       5       3
 
BseYI C'CCAG_C

Cuts at:      0     598     769    1211    1382    1526    1533    1713    1857 
   Size:        598     171     442     171     144       7     180     144

Cuts at:   1857    3221    3928    4311    4566    4635 
   Size:       1364     707     383     255      69

  Fragments arranged by size: 

                1364     707     598     442     383     255     180     171
                 171     144     144      69       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1804    2272    2882    3673    4027    4264    4407    4635 
   Size:       1804     468     610     791     354     237     143     228

  Fragments arranged by size: 

                1804     791     610     468     354     237     228     143
            
 
BsiEI CG_ry'CG

Cuts at:      0    2670    2896    4635 
   Size:       2670     226    1739

  Fragments arranged by size: 

                2670    1739     226
 
BsiHKAI G_wGCw'C

Cuts at:      0     530     821     917    1043    1058    1331    1901    2009 
   Size:        530     291      96     126      15     273     570     108

Cuts at:   2009    2234    2462    2567    2929    3395    4184    4635 
   Size:        225     228     105     362     466     789     451

  Fragments arranged by size: 

                 789     570     530     466     451     362     291     273
                 228     225     126     108     105      96      15
 
BsmI GAATG_Cn'

Cuts at:      0     773    3759    4635 
   Size:        773    2986     876

  Fragments arranged by size: 

                2986     876     773
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     139    3959    4635 
   Size:        139    3820     676

  Fragments arranged by size: 

                3820     676     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     530     596     751     821     917     996    1043    1058 
   Size:        530      66     155      70      96      79      47      15

Cuts at:   1058    1137    1331    1526    1720    1901    2009    2234    2397 
   Size:         79     194     195     194     181     108     225     163

Cuts at:   2397    2462    2567    2929    3305    3395    3544    3636    4152 
   Size:         65     105     362     376      90     149      92     516

Cuts at:   4152    4177    4184    4301    4318    4411    4538    4592    4635 
   Size:         25       7     117      17      93     127      54      43

  Fragments arranged by size: 

                 530     516     376     362     225     195     194     194
                 181     163     155     149     127     117     108     105
                  96      93      92      90      79      79      70      66
                  65      54      47      43      25      17      15       7
            
 
BspEI T'CCGG_A

Cuts at:      0     863    4635 
   Size:        863    3772
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     839    1584    1879    2615    3490    3575    4635 
   Size:        839     745     295     736     875      85    1060

  Fragments arranged by size: 

                1060     875     839     745     736     295      85
 
BsrBI CCG'CTC

Cuts at:      0     741    1289    1395    1500    1785    2700    4154    4635 
   Size:        741     548     106     105     285     915    1454     481

  Fragments arranged by size: 

                1454     915     741     548     481     285     106     105
            
 
BsrFI r'CCGG_y

Cuts at:      0     896    1767    2891    4273    4635 
   Size:        896     871    1124    1382     362

  Fragments arranged by size: 

                1382    1124     896     871     362
 
BsrGI T'GTAC_A

Cuts at:      0     389    4635 
   Size:        389    4246
 
BssHII G'CGCG_C

Cuts at:      0     255    4635 
   Size:        255    4380
 
BssSI C'ACGA_G

Cuts at:      0     910    1069    1375    3304    4635 
   Size:        910     159     306    1929    1331

  Fragments arranged by size: 

                1929    1331     910     306     159
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3411    4031    4635 
   Size:       3411     620     604

  Fragments arranged by size: 

                3411     620     604
 
BstBI TT'CG_AA

Cuts at:      0     174    4635 
   Size:        174    4461
 
BstEII G'GTnAC_C

Cuts at:      0    2170    4635 
   Size:       2170    2465
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     660    2182    3253    3811    3855    4371    4635 
   Size:        660    1522    1071     558      44     516     264

  Fragments arranged by size: 

                1522    1071     660     558     516     264      44
 
BstYI r'GATC_y

Cuts at:      0    2618    4472    4635 
   Size:       2618    1854     163

  Fragments arranged by size: 

                2618    1854     163
 
Bsu36I CC'TnA_GG

Cuts at:      0    3029    3970    4635 
   Size:       3029     941     665

  Fragments arranged by size: 

                3029     941     665
 
BtgI C'CryG_G

Cuts at:      0     575     696    1322    1851    2285    3368    4238    4530 
   Size:        575     121     626     529     434    1083     870     292

Cuts at:   4530    4592    4635 
   Size:         62      43

  Fragments arranged by size: 

                1083     870     626     575     529     434     292     121
                  62      43
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1169    4635 
   Size:       1169    3466
 
BtsI GCAGTG_nn'

Cuts at:      0    3393    3409    3685    4635 
   Size:       3393      16     276     950

  Fragments arranged by size: 

                3393     950     276      16
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1674    3813    4635 
   Size:       1674    2139     822

  Fragments arranged by size: 

                2139    1674     822
 
EaeI y'GGCC_r

Cuts at:      0     488    2846    2893    2930    4635 
   Size:        488    2358      47      37    1705

  Fragments arranged by size: 

                2358    1705     488      47      37
 
EagI C'GGCC_G

Cuts at:      0    2893    4635 
   Size:       2893    1742
 
EarI CTCTTCn'nnn_

Cuts at:      0     839    1487    2041    2480    2975    4219    4635 
   Size:        839     648     554     439     495    1244     416

  Fragments arranged by size: 

                1244     839     648     554     495     439     416
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     811    2194    4635 
   Size:        811    1383    2441

  Fragments arranged by size: 

                2441    1383     811
 
EcoICRI GAG'CTC

Cuts at:      0     528     819     915    1041    1329    2007    4635 
   Size:        528     291      96     126     288     678    2628

  Fragments arranged by size: 

                2628     678     528     291     288     126      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     313     361     430     514     538     640     703 
   Size:        202     111      48      69      84      24     102      63

Cuts at:    703     823    1033    1354    1468    1609    1771    1860    1903 
   Size:        120     210     321     114     141     162      89      43

Cuts at:   1903    1995    2124    2377    2929    3167    3551    3718    3890 
   Size:         92     129     253     552     238     384     167     172

Cuts at:   3890    4191    4635 
   Size:        301     444

  Fragments arranged by size: 

                 552     444     384     321     301     253     238     210
                 202     172     167     162     141     129     120     114
                 111     102      92      89      84      69      63      48
                  43      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     184    1003    1591    1705    3028    3603    4635 
   Size:        184     819     588     114    1323     575    1032

  Fragments arranged by size: 

                1323    1032     819     588     575     184     114
 
EcoO109I rG'GnC_Cy

Cuts at:      0     324     593     747     992     993    1228    1474    2540 
   Size:        324     269     154     245       1     235     246    1066

Cuts at:   2540    3135    3511    3562    3815    3830    4248    4535    4588 
   Size:        595     376      51     253      15     418     287      53

Cuts at:   4588    4635 
   Size:         47

  Fragments arranged by size: 

                1066     595     418     376     324     287     269     253
                 246     245     235     154      53      51      47      15
                   1
 
EcoRI G'AATT_C

Cuts at:      0    2713    4635 
   Size:       2713    1922
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     423    2559    2733    3073    4443    4635 
   Size:        423    2136     174     340    1370     192

  Fragments arranged by size: 

                2136    1370     423     340     192     174
 
FseI GG_CCGG'CC

Cuts at:      0    1771    4635 
   Size:       1771    2864
 
FspI TGC'GCA

Cuts at:      0     478    3475    4635 
   Size:        478    2997    1160

  Fragments arranged by size: 

                2997    1160     478
 
HaeII r_GCGC'y

Cuts at:      0    1104    1410    2342    3642    4635 
   Size:       1104     306     932    1300     993

  Fragments arranged by size: 

                1300    1104     993     932     306
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1000    1032    1258    1290    1603    1635    1714    1746 
   Size:       1000      32     226      32     313      32      79      32

Cuts at:   1746    1996    2028    2247    2279    2857    2889    2911    2943 
   Size:        250      32     219      32     578      32      22      32

Cuts at:   2943    2944    2976    4189    4221    4546    4578    4635 
   Size:          1      32    1213      32     325      32      57

  Fragments arranged by size: 

                1213    1000     578     325     313     250     226     219
                  79      57      32      32      32      32      32      32
                  32      32      32      32      32      22       1
 
HincII GTy'rAC

Cuts at:      0    3483    4635 
   Size:       3483    1152
 
KasI G'GCGC_C

Cuts at:      0    3638    4635 
   Size:       3638     997
 
KpnI G_GTAC'C

Cuts at:      0    3345    4635 
   Size:       3345    1290
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     312    1115    2897    4635 
   Size:        312     803    1782    1738

  Fragments arranged by size: 

                1782    1738     803     312
 
MscI TGG'CCA

Cuts at:      0     490    2848    4635 
   Size:        490    2358    1787

  Fragments arranged by size: 

                2358    1787     490
 
MslI CAynn'nnrTG

Cuts at:      0    1904    2198    3251    3853    4369    4414    4635 
   Size:       1904     294    1053     602     516      45     221

  Fragments arranged by size: 

                1904    1053     602     516     294     221      45
 
MspA1I CmG'CkG

Cuts at:      0      45    1046    1386    1530    1749    2287    2331    2481 
   Size:         45    1001     340     144     219     538      44     150

Cuts at:   2481    2527    2898    3887    4635 
   Size:         46     371     989     748

  Fragments arranged by size: 

                1001     989     748     538     371     340     219     150
                 144      46      45      44
 
NaeI GCC'GGC

Cuts at:      0    1769    4275    4635 
   Size:       1769    2506     360

  Fragments arranged by size: 

                2506    1769     360
 
NarI GG'CG_CC

Cuts at:      0    3639    4635 
   Size:       3639     996
 
NcoI C'CATG_G

Cuts at:      0    4592    4635 
   Size:       4592      43
 
NgoMIV G'CCGG_C

Cuts at:      0    1767    4273    4635 
   Size:       1767    2506     362

  Fragments arranged by size: 

                2506    1767     362
 
NspI r_CATG'y

Cuts at:      0     726    2835    4031    4501    4635 
   Size:        726    2109    1196     470     134

  Fragments arranged by size: 

                2109    1196     726     470     134
 
PasI CC'CwG_GG

Cuts at:      0    3669    4625    4635 
   Size:       3669     956      10

  Fragments arranged by size: 

                3669     956      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1533    1857    3928    4566    4635 
   Size:       1533     324    2071     638      69

  Fragments arranged by size: 

                2071    1533     638     324      69
 
PfoI T'CCnGG_A

Cuts at:      0    1586    3817    3961    4635 
   Size:       1586    2231     144     674

  Fragments arranged by size: 

                2231    1586     674     144
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2995    3027    3563    3595    4635 
   Size:       2995      32     536      32    1040

  Fragments arranged by size: 

                2995    1040     536      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3511    3815    3830    4248    4635 
   Size:       3511     304      15     418     387

  Fragments arranged by size: 

                3511     418     387     304      15
 
PspOMI G'GGCC_C

Cuts at:      0     592     747     992    1716    4148    4314    4534    4588 
   Size:        592     155     245     724    2432     166     220      54

Cuts at:   4588    4635 
   Size:         47

  Fragments arranged by size: 

                2432     724     592     245     220     166     155      54
                  47
 
PstI C_TGCA'G

Cuts at:      0      50     101     836    1004    1031    1067    1580    2282 
   Size:         50      51     735     168      27      36     513     702

Cuts at:   2282    2411    4064    4635 
   Size:        129    1653     571

  Fragments arranged by size: 

                1653     735     702     571     513     168     129      51
                  50      36      27
 
PvuII CAG'CTG

Cuts at:      0      45    1530    1749    2331    2481    4635 
   Size:         45    1485     219     582     150    2154

  Fragments arranged by size: 

                2154    1485     582     219     150      45
 
SacI G_AGCT'C

Cuts at:      0     530     821     917    1043    1331    2009    4635 
   Size:        530     291      96     126     288     678    2626

  Fragments arranged by size: 

                2626     678     530     291     288     126      96
 
SacII CC_GC'GG

Cuts at:      0    2288    4635 
   Size:       2288    2347
 
SalI G'TCGA_C

Cuts at:      0    3481    4635 
   Size:       3481    1154
 
SanDI GG'GwC_CC

Cuts at:      0    3511    3815    3830    4635 
   Size:       3511     304      15     805

  Fragments arranged by size: 

                3511     805     304      15
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2975    4635 
   Size:       2975    1660
 
SbfI CC_TGCA'GG

Cuts at:      0    1004    4635 
   Size:       1004    3631
 
SfcI C'TryA_G

Cuts at:      0      46      97     832    1000    1027    1063    1576    2278 
   Size:         46      51     735     168      27      36     513     702

Cuts at:   2278    2407    4060    4635 
   Size:        129    1653     575

  Fragments arranged by size: 

                1653     735     702     575     513     168     129      51
                  46      36      27
 
SfoI GGC'GCC

Cuts at:      0    3640    4635 
   Size:       3640     995
 
SmaI CCC'GGG

Cuts at:      0     607     752     943    1147    1342    1681    2248    3545 
   Size:        607     145     191     204     195     339     567    1297

Cuts at:   3545    3616    4541    4635 
   Size:         71     925      94

  Fragments arranged by size: 

                1297     925     607     567     339     204     195     191
                 145      94      71
 
SmlI C'TyrA_G

Cuts at:      0     235     703    1911    2461    3193    3714    4635 
   Size:        235     468    1208     550     732     521     921

  Fragments arranged by size: 

                1208     921     732     550     521     468     235
 
SphI G_CATG'C

Cuts at:      0    2835    4635 
   Size:       2835    1800
 
StuI AGG'CCT

Cuts at:      0    1582    2350    4635 
   Size:       1582     768    2285

  Fragments arranged by size: 

                2285    1582     768
 
StyI C'CwwG_G

Cuts at:      0    3554    4592    4635 
   Size:       3554    1038      43

  Fragments arranged by size: 

                3554    1038      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     783    2219    3249    3298    4271    4635 
   Size:        783    1436    1030      49     973     364

  Fragments arranged by size: 

                1436    1030     973     783     364      49
 
TatI w'GTAC_w

Cuts at:      0     389     536    3197    4635 
   Size:        389     147    2661    1438

  Fragments arranged by size: 

                2661    1438     389     147
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2434    2466    2704    2736    4635 
   Size:       2434      32     238      32    1899

  Fragments arranged by size: 

                2434    1899     238      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1698    2849    4635 
   Size:       1698    1151    1786

  Fragments arranged by size: 

                1786    1698    1151
 
XmaI C'CCGG_G

Cuts at:      0     605     750     941    1145    1340    1679    2246    3543 
   Size:        605     145     191     204     195     339     567    1297

Cuts at:   3543    3614    4539    4635 
   Size:         71     925      96

  Fragments arranged by size: 

                1297     925     605     567     339     204     195     191
                 145      96      71
 
XmnI GAAnn'nnTTC

Cuts at:      0    1561    2029    2713    4635 
   Size:       1561     468     684    1922

  Fragments arranged by size: 

                1922    1561     684     468
 
ZraI GAC'GTC

Cuts at:      0    1274    4106    4635 
   Size:       1274    2832     529

  Fragments arranged by size: 

                2832    1274     529
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI     SfcI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   AflIII     AgeI     AloI     AscI    AsiSI    AvrII     BaeI    BciVI
    BfrBI    BglII     BmtI    BsaAI    BsaBI    BsiWI    BspHI    BsrDI
  BstZ17I     ClaI    CspCI     DraI   DraIII    EcoRV    FspAI  HindIII
     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
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