Restriction map for pF1KA0765
 (Linear) (Six-base) MAPSORT of: KIAA0765.seq  Check: 9215  from: 1  to: 2796



KIAA0765.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AclI AA'CG_TT

Cuts at:      0     508    2796 
   Size:        508    2288
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1554    1666    2249    2796 
   Size:       1554     112     583     547

  Fragments arranged by size: 

                1554     583     547     112
 
AflIII A'CryG_T

Cuts at:      0    1332    1722    2796 
   Size:       1332     390    1074

  Fragments arranged by size: 

                1332    1074     390
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2044    2796 
   Size:       2044     752
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     459     491    2288    2320    2796 
   Size:        459      32    1797      32     476

  Fragments arranged by size: 

                1797     476     459      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2737    2796 
   Size:       2737      59
 
ApaI G_GGCC'C

Cuts at:      0    2365    2796 
   Size:       2365     431
 
ApoI r'AATT_y

Cuts at:      0     798    2370    2796 
   Size:        798    1572     426

  Fragments arranged by size: 

                1572     798     426
 
AvaI C'yCGr_G

Cuts at:      0    2406    2717    2796 
   Size:       2406     311      79

  Fragments arranged by size: 

                2406     311      79
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     350     383    2796 
   Size:        350      33    2413

  Fragments arranged by size: 

                2413     350      33
 
BanI G'GyrC_C

Cuts at:      0     756    2796 
   Size:        756    2040
 
BanII G_rGCy'C

Cuts at:      0     505     980    2365    2796 
   Size:        505     475    1385     431

  Fragments arranged by size: 

                1385     505     475     431
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     378    2796 
   Size:        378    2418
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1245    2704    2796 
   Size:       1245    1459      92

  Fragments arranged by size: 

                1459    1245      92
 
Bme1580I G_kGCm'C

Cuts at:      0     671    1638    2365    2445    2796 
   Size:        671     967     727      80     351

  Fragments arranged by size: 

                 967     727     671     351      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     127     386     676    1235    1696    1982    2072    2796 
   Size:        127     259     290     559     461     286      90     724

  Fragments arranged by size: 

                 724     559     461     290     286     259     127      90
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     806    1175    2201    2796 
   Size:        620     186     369    1026     595

  Fragments arranged by size: 

                1026     620     595     369     186
 
Bpu10I CC'TnA_GC

Cuts at:      0     330    2394    2796 
   Size:        330    2064     402

  Fragments arranged by size: 

                2064     402     330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1043    2796 
   Size:       1043    1753
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31    2796 
   Size:         31    2765
 
BsaAI yAC'GTr

Cuts at:      0    1795    2796 
   Size:       1795    1001
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     459     489     522     552     636     666    2796 
   Size:        459      30      33      30      84      30    2130

  Fragments arranged by size: 

                2130     459      84      33      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     464     623    1202    2204    2796 
   Size:        464     159     579    1002     592

  Fragments arranged by size: 

                1002     592     579     464     159
 
BseYI C'CCAG_C

Cuts at:      0    2017    2796 
   Size:       2017     779
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2012    2027    2072    2102    2117    2796 
   Size:       2012      15      45      30      15     679

  Fragments arranged by size: 

                2012     679      45      30      15      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     792    2796 
   Size:        792    2004
 
BsmI GAATG_Cn'

Cuts at:      0     935    1097    1958    2078    2796 
   Size:        935     162     861     120     718

  Fragments arranged by size: 

                 935     861     718     162     120
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     671     792     980    1638    2365    2445    2796 
   Size:        505     166     121     188     658     727      80     351

  Fragments arranged by size: 

                 727     658     505     351     188     166     121      80
            
 
BspHI T'CATG_A

Cuts at:      0     420    2796 
   Size:        420    2376
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     774    2796 
   Size:        774    2022
 
BsrDI GCAATG_nn'

Cuts at:      0     646     950    2796 
   Size:        646     304    1846

  Fragments arranged by size: 

                1846     646     304
 
BsrGI T'GTAC_A

Cuts at:      0    1719    2796 
   Size:       1719    1077
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     930    2586    2796 
   Size:        930    1656     210

  Fragments arranged by size: 

                1656     930     210
 
BstBI TT'CG_AA

Cuts at:      0    1551    2796 
   Size:       1551    1245
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2521    2796 
   Size:       2521     275
 
BtgI C'CryG_G

Cuts at:      0      47    2699    2796 
   Size:         47    2652      97

  Fragments arranged by size: 

                2652      97      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     598    2796 
   Size:        598    2198
 
BtsI GCAGTG_nn'

Cuts at:      0     709     882     998    2796 
   Size:        709     173     116    1798

  Fragments arranged by size: 

                1798     709     173     116
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2319    2354    2796 
   Size:       2319      35     442

  Fragments arranged by size: 

                2319     442      35
 
DraI TTT'AAA

Cuts at:      0    1350    1776    2663    2796 
   Size:       1350     426     887     133

  Fragments arranged by size: 

                1350     887     426     133
 
DraIII CAC_nnn'GTG

Cuts at:      0     394    2796 
   Size:        394    2402
 
EarI CTCTTCn'nnn_

Cuts at:      0    2102    2796 
   Size:       2102     694
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     734    2796 
   Size:        734    2062
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     806    1175    1554    1666    2201    2249    2796 
   Size:        620     186     369     379     112     535      48     547

  Fragments arranged by size: 

                 620     547     535     379     369     186     112      48
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     329    1898    1928    2096    2216    2291    2796 
   Size:        329    1569      30     168     120      75     505

  Fragments arranged by size: 

                1569     505     329     168     120      75      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1186    2234    2361    2362    2796 
   Size:       1186    1048     127       1     434

  Fragments arranged by size: 

                1186    1048     434     127       1
 
FspI TGC'GCA

Cuts at:      0     175    2796 
   Size:        175    2621
 
FspAI rTGC'GCAy

Cuts at:      0     175    2796 
   Size:        175    2621
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     576     608     852     884    1148    1180    1584    1603 
   Size:        576      32     244      32     264      32     404      19

Cuts at:   1603    1616    1635    2796 
   Size:         13      19    1161

  Fragments arranged by size: 

                1161     576     404     264     244      32      32      32
                  19      19      13
 
HincII GTy'rAC

Cuts at:      0     849    1617    2796 
   Size:        849     768    1179

  Fragments arranged by size: 

                1179     849     768
 
HindIII A'AGCT_T

Cuts at:      0    1073    1838    2796 
   Size:       1073     765     958

  Fragments arranged by size: 

                1073     958     765
 
HpaI GTT'AAC

Cuts at:      0     849    2796 
   Size:        849    1947
 
MfeI C'AATT_G

Cuts at:      0      32    2796 
   Size:         32    2764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     395    2250    2796 
   Size:        395    1855     546

  Fragments arranged by size: 

                1855     546     395
 
MslI CAynn'nnrTG

Cuts at:      0    2456    2519    2796 
   Size:       2456      63     277

  Fragments arranged by size: 

                2456     277      63
 
MspA1I CmG'CkG

Cuts at:      0    2734    2796 
   Size:       2734      62
 
NcoI C'CATG_G

Cuts at:      0      47    2699    2796 
   Size:         47    2652      97

  Fragments arranged by size: 

                2652      97      47
 
NspI r_CATG'y

Cuts at:      0     732    1336    1726    2116    2589    2796 
   Size:        732     604     390     390     473     207

  Fragments arranged by size: 

                 732     604     473     390     390     207
 
PasI CC'CwG_GG

Cuts at:      0    2446    2796 
   Size:       2446     350
 
PciI A'CATG_T

Cuts at:      0    1332    1722    2796 
   Size:       1332     390    1074

  Fragments arranged by size: 

                1332    1074     390
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2473    2796 
   Size:       2473     323
 
PfoI T'CCnGG_A

Cuts at:      0    1947    2217    2796 
   Size:       1947     270     579

  Fragments arranged by size: 

                1947     579     270
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     459     491    2796 
   Size:        459      32    2305

  Fragments arranged by size: 

                2305     459      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1186    2796 
   Size:       1186    1610
 
PspOMI G'GGCC_C

Cuts at:      0    2361    2796 
   Size:       2361     435
 
PstI C_TGCA'G

Cuts at:      0    1565    1944    2796 
   Size:       1565     379     852

  Fragments arranged by size: 

                1565     852     379
 
PvuII CAG'CTG

Cuts at:      0    2734    2796 
   Size:       2734      62
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2102    2796 
   Size:       2102     694
 
SfcI C'TryA_G

Cuts at:      0    1561    1940    2126    2762    2796 
   Size:       1561     379     186     636      34

  Fragments arranged by size: 

                1561     636     379     186      34
 
SmaI CCC'GGG

Cuts at:      0    2408    2796 
   Size:       2408     388
 
SmlI C'TyrA_G

Cuts at:      0    1058    2796 
   Size:       1058    1738
 
SphI G_CATG'C

Cuts at:      0     732    2116    2796 
   Size:        732    1384     680

  Fragments arranged by size: 

                1384     732     680
 
SspI AAT'ATT

Cuts at:      0    1650    2796 
   Size:       1650    1146
 
StuI AGG'CCT

Cuts at:      0    2012    2253    2760    2796 
   Size:       2012     241     507      36

  Fragments arranged by size: 

                2012     507     241      36
 
StyI C'CwwG_G

Cuts at:      0      47    1899    2699    2796 
   Size:         47    1852     800      97

  Fragments arranged by size: 

                1852     800      97      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109     405     714     732    2446    2577    2796 
   Size:        109     296     309      18    1714     131     219

  Fragments arranged by size: 

                1714     309     296     219     131     109      18
 
TatI w'GTAC_w

Cuts at:      0    1478    1719    2796 
   Size:       1478     241    1077

  Fragments arranged by size: 

                1478    1077     241
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     522     554    1688    1720    2796 
   Size:        522      32    1134      32    1076

  Fragments arranged by size: 

                1134    1076     522      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      67    2796 
   Size:         67    2729
 
XmaI C'CCGG_G

Cuts at:      0    2406    2796 
   Size:       2406     390
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI     ApoI
     AvaI     BaeI     BanI    BanII    BciVI     BglI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
   BstAPI    BstBI    BstXI     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MslI   MspA1I
     NcoI     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SapI     SfcI     SmaI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstYI  BstZ17I
   Bsu36I     ClaI     DrdI     EaeI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    HaeII     KasI     KpnI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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