Restriction map for pF1KA0743
 (Linear) (Six-base) MAPSORT of: KIAA0743.seq  Check: 5911  from: 1  to: 3183



KIAA0743.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2044    2448    3183 
   Size:       2044     404     735

  Fragments arranged by size: 

                2044     735     404
 
AccI GT'mk_AC

Cuts at:      0     395    2728    3183 
   Size:        395    2333     455

  Fragments arranged by size: 

                2333     455     395
 
Acc65I G'GTAC_C

Cuts at:      0     506    3183 
   Size:        506    2677
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1477    1525    2229    3183 
   Size:       1477      48     704     954

  Fragments arranged by size: 

                1477     954     704      48
 
AflII C'TTAA_G

Cuts at:      0    1243    3183 
   Size:       1243    1940
 
AflIII A'CryG_T

Cuts at:      0     617    3183 
   Size:        617    2566
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3045    3183 
   Size:       3045     138
 
AleI CACnn'nnGTG

Cuts at:      0    1801    2254    3183 
   Size:       1801     453     929

  Fragments arranged by size: 

                1801     929     453
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     791    3183 
   Size:        791    2392
 
AvaI C'yCGr_G

Cuts at:      0    1760    1913    3183 
   Size:       1760     153    1270

  Fragments arranged by size: 

                1760    1270     153
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1268    1301    3183 
   Size:       1268      33    1882

  Fragments arranged by size: 

                1882    1268      33
 
BanI G'GyrC_C

Cuts at:      0     457     506     892    1825    3183 
   Size:        457      49     386     933    1358

  Fragments arranged by size: 

                1358     933     457     386      49
 
BanII G_rGCy'C

Cuts at:      0      10     289     320     454    2588    3133    3183 
   Size:         10     279      31     134    2134     545      50

  Fragments arranged by size: 

                2134     545     279     134      50      31      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2340    2861    3183 
   Size:       2340     521     322

  Fragments arranged by size: 

                2340     521     322
 
BbvCI CC'TCA_GC

Cuts at:      0    1271    3183 
   Size:       1271    1912
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1198    1232    2694    2728    3183 
   Size:       1198      34    1462      34     455

  Fragments arranged by size: 

                1462    1198     455      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     892    3183 
   Size:        892    2291
 
BclI T'GATC_A

Cuts at:      0    1700    3183 
   Size:       1700    1483
 
BfrBI ATG'CAT

Cuts at:      0     987    3183 
   Size:        987    2196
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     912    2021    3183 
   Size:        912    1109    1162

  Fragments arranged by size: 

                1162    1109     912
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151    1044    1108    1137    1350    2276    2296    3183 
   Size:        151     893      64      29     213     926      20     887

  Fragments arranged by size: 

                 926     893     887     213     151      64      29      20
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     937    1271    1525    3183 
   Size:        937     334     254    1658

  Fragments arranged by size: 

                1658     937     334     254
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     229    1162    1283    3183 
   Size:        229     933     121    1900

  Fragments arranged by size: 

                1900     933     229     121
 
BsaAI yAC'GTr

Cuts at:      0     702    2153    3180    3183 
   Size:        702    1451    1027       3

  Fragments arranged by size: 

                1451    1027     702       3
 
BsaBI GATnn'nnATC

Cuts at:      0     172    3183 
   Size:        172    3011
 
BsaHI Gr'CG_yC

Cuts at:      0    1959    2559    3183 
   Size:       1959     600     624

  Fragments arranged by size: 

                1959     624     600
 
BsaWI w'CCGG_w

Cuts at:      0     895    3183 
   Size:        895    2288
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1135    1165    3183 
   Size:       1135      30    2018

  Fragments arranged by size: 

                2018    1135      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     406    2398    3183 
   Size:        406    1992     785

  Fragments arranged by size: 

                1992     785     406
 
BseYI C'CCAG_C

Cuts at:      0       3    1025    2212    3183 
   Size:          3    1022    1187     971

  Fragments arranged by size: 

                1187    1022     971       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     870    3183 
   Size:        870    2313
 
BsiHKAI G_wGCw'C

Cuts at:      0    2292    3133    3183 
   Size:       2292     841      50

  Fragments arranged by size: 

                2292     841      50
 
BsmI GAATG_Cn'

Cuts at:      0     777    2798    3183 
   Size:        777    2021     385

  Fragments arranged by size: 

                2021     777     385
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3058    3183 
   Size:       3058     125
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     289     320     454    2292    2588    3133    3183 
   Size:         10     279      31     134    1838     296     545      50

  Fragments arranged by size: 

                1838     545     296     279     134      50      31      10
            
 
BspHI T'CATG_A

Cuts at:      0    1331    3110    3183 
   Size:       1331    1779      73

  Fragments arranged by size: 

                1779    1331      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     922    2044    2448    3183 
   Size:        922    1122     404     735

  Fragments arranged by size: 

                1122     922     735     404
 
BsrBI CCG'CTC

Cuts at:      0    1997    3140    3183 
   Size:       1997    1143      43

  Fragments arranged by size: 

                1997    1143      43
 
BsrDI GCAATG_nn'

Cuts at:      0    1632    1634    2142    3183 
   Size:       1632       2     508    1041

  Fragments arranged by size: 

                1632    1041     508       2
 
BsrFI r'CCGG_y

Cuts at:      0     889    2498    3183 
   Size:        889    1609     685

  Fragments arranged by size: 

                1609     889     685
 
BsrGI T'GTAC_A

Cuts at:      0     956    1883    3020    3183 
   Size:        956     927    1137     163

  Fragments arranged by size: 

                1137     956     927     163
 
BssSI C'ACGA_G

Cuts at:      0    1903    3183 
   Size:       1903    1280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1836    2581    3183 
   Size:       1836     745     602

  Fragments arranged by size: 

                1836     745     602
 
BstYI r'GATC_y

Cuts at:      0     596    3183 
   Size:        596    2587
 
BtgI C'CryG_G

Cuts at:      0     407     977    1253    2237    2891    3183 
   Size:        407     570     276     984     654     292

  Fragments arranged by size: 

                 984     654     570     407     292     276
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1470    3183 
   Size:       1470    1713
 
BtsI GCAGTG_nn'

Cuts at:      0     823    3183 
   Size:        823    2360
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1886    1921    3183 
   Size:       1886      35    1262

  Fragments arranged by size: 

                1886    1262      35
 
EaeI y'GGCC_r

Cuts at:      0     209    1377    2194    2470    3141    3183 
   Size:        209    1168     817     276     671      42

  Fragments arranged by size: 

                1168     817     671     276     209      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     340    1544    1570    1612    1991    2569    3130    3183 
   Size:        340    1204      26      42     379     578     561      53

  Fragments arranged by size: 

                1204     578     561     379     340      53      42      26
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3078    3183 
   Size:       3078     105
 
EcoICRI GAG'CTC

Cuts at:      0    3131    3183 
   Size:       3131      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151    1044    1108    1137    1350    1477    1525    2229 
   Size:        151     893      64      29     213     127      48     704

Cuts at:   2229    2276    2296    3183 
   Size:         47      20     887

  Fragments arranged by size: 

                 893     887     704     213     151     127      64      48
                  47      29      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      97     918    3183 
   Size:         97     821    2265

  Fragments arranged by size: 

                2265     821      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2023    2921    3047    3183 
   Size:       2023     898     126     136

  Fragments arranged by size: 

                2023     898     136     126
 
HaeII r_GCGC'y

Cuts at:      0    2150    3183 
   Size:       2150    1033
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468     520     552     991    1023    3031    3063 
   Size:        436      32      52      32     439      32    2008      32

Cuts at:   3063    3183 
   Size:        120

  Fragments arranged by size: 

                2008     439     436     120      52      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1122    1250    2367    2507    3183 
   Size:       1122     128    1117     140     676

  Fragments arranged by size: 

                1122    1117     676     140     128
 
HpaI GTT'AAC

Cuts at:      0    1122    1250    2507    3183 
   Size:       1122     128    1257     676

  Fragments arranged by size: 

                1257    1122     676     128
 
KpnI G_GTAC'C

Cuts at:      0     510    3183 
   Size:        510    2673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2649    3183 
   Size:       2649     534
 
MscI TGG'CCA

Cuts at:      0     211    1379    2196    2472    3183 
   Size:        211    1168     817     276     711

  Fragments arranged by size: 

                1168     817     711     276     211
 
MslI CAynn'nnrTG

Cuts at:      0     166     466     958     976     982    1555    1801    2254 
   Size:        166     300     492      18       6     573     246     453

Cuts at:   2254    3109    3183 
   Size:        855      74

  Fragments arranged by size: 

                 855     573     492     453     300     246     166      74
                  18       6
 
MspA1I CmG'CkG

Cuts at:      0      49     756    3183 
   Size:         49     707    2427

  Fragments arranged by size: 

                2427     707      49
 
NaeI GCC'GGC

Cuts at:      0    2500    3183 
   Size:       2500     683
 
NcoI C'CATG_G

Cuts at:      0     977    3183 
   Size:        977    2206
 
NgoMIV G'CCGG_C

Cuts at:      0    2498    3183 
   Size:       2498     685
 
NsiI A_TGCA'T

Cuts at:      0     989    3183 
   Size:        989    2194
 
NspI r_CATG'y

Cuts at:      0     621     957    1884    2076    3183 
   Size:        621     336     927     192    1107

  Fragments arranged by size: 

                1107     927     621     336     192
 
PciI A'CATG_T

Cuts at:      0     617    3183 
   Size:        617    2566
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2299    3183 
   Size:       2299     884
 
PfoI T'CCnGG_A

Cuts at:      0     599    2292    2936    3183 
   Size:        599    1693     644     247

  Fragments arranged by size: 

                1693     644     599     247
 
PpuMI rG'GwC_Cy

Cuts at:      0    2023    3047    3183 
   Size:       2023    1024     136

  Fragments arranged by size: 

                2023    1024     136
 
PshAI GACnn'nnGTC

Cuts at:      0     125    3183 
   Size:        125    3058
 
PsiI TTA'TAA

Cuts at:      0     110    3183 
   Size:        110    3073
 
PstI C_TGCA'G

Cuts at:      0    1388    2459    3183 
   Size:       1388    1071     724

  Fragments arranged by size: 

                1388    1071     724
 
PvuII CAG'CTG

Cuts at:      0     756    3183 
   Size:        756    2427
 
SacI G_AGCT'C

Cuts at:      0    3133    3183 
   Size:       3133      50
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1991    3130    3183 
   Size:       1991    1139      53

  Fragments arranged by size: 

                1991    1139      53
 
SbfI CC_TGCA'GG

Cuts at:      0    2459    3183 
   Size:       2459     724
 
ScaI AGT'ACT

Cuts at:      0    2609    3183 
   Size:       2609     574
 
SfcI C'TryA_G

Cuts at:      0    1384    2455    2492    2789    3183 
   Size:       1384    1071      37     297     394

  Fragments arranged by size: 

                1384    1071     394     297      37
 
SmlI C'TyrA_G

Cuts at:      0    1243    1913    3183 
   Size:       1243     670    1270

  Fragments arranged by size: 

                1270    1243     670
 
SnaBI TAC'GTA

Cuts at:      0    2153    3183 
   Size:       2153    1030
 
SphI G_CATG'C

Cuts at:      0    2076    3183 
   Size:       2076    1107
 
SspI AAT'ATT

Cuts at:      0    2640    3183 
   Size:       2640     543
 
StyI C'CwwG_G

Cuts at:      0     977    1875    2568    3183 
   Size:        977     898     693     615

  Fragments arranged by size: 

                 977     898     693     615
 
TatI w'GTAC_w

Cuts at:      0     431     956    1883    2607    2878    3020    3026    3183 
   Size:        431     525     927     724     271     142       6     157

  Fragments arranged by size: 

                 927     724     525     431     271     157     142       6
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2157    2189    3183 
   Size:       2157      32     994

  Fragments arranged by size: 

                2157     994      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1495    3183 
   Size:       1495    1688
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1033    2457    2754    3183 
   Size:       1033    1424     297     429

  Fragments arranged by size: 

                1424    1033     429     297
 
XhoI C'TCGA_G

Cuts at:      0    1913    3183 
   Size:       1913    1270
 
XmnI GAAnn'nnTTC

Cuts at:      0     871    3183 
   Size:        871    2312
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AleI
     AlfI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   HincII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI
    PpuMI    PshAI     PsiI     PstI    PvuII     SacI     SapI     SbfI
     ScaI     SfcI     SmlI    SnaBI     SphI     SspI     StyI     TatI
     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsiEI
    BsiWI    BspEI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     KasI     MfeI     MluI     NarI
     NdeI     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SrfI     StuI     SwaI    TaqII     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com