Restriction map for pF1KA0737
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaHI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      Bst1107I    BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
KasI        KpnI        KspI        MaeII       MluI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        572  TCCGGA               T^CCGGA
AvaI         1190  CCCGAG               C^[CT]CG[GA]G
AvrII         468  CCTAGG               C^CTAGG
BalI          168  TGGCCA               TGG^CCA
BamHI         915  GGATCC               G^GATCC
BbsI          594  GAAGAC               GAAGAC(2/6)
BcnI           10  CCCGG                CC^[GC]GG
BlnI          468  CCTAGG               C^CTAGG
BpuAI         594  GAAGAC               GAAGAC(2/6)
BsaBI         133  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1682  GGTCTC               GGTCTC(1/5)
BsaWI         572  TCCGGA               [AT]^CCGG[AT]
BseAI         572  TCCGGA               T^CCGGA
BsoBI        1190  CCCGAG               C^[CT]CG[GA]G
BspEI         572  TCCGGA               T^CCGGA
BspMI         492  ACCTGC               ACCTGC(4/8)
BsrBI        1549  GAGCGG               CCGCTC(-3/-3)
BsrDI        1778  GCAATG               GCAATG(2/0)
BssSI         550  CACGAG               CACGAG(-5/-1)
BstBI        1439  TTCGAA               TT^CGAA
BstEII       1182  GGTGACC              G^GT[ACGT]ACC
BstPI        1182  GGTGACC              G^GT[ACGT]ACC
BstXI         314  CCATGGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        1272  GCAGGC               GC[ACGT]^[ACGT]GC
EaeI          168  TGGCCA               [CT]^GGCC[GA]
EcoO65I      1182  GGTGACC              G^GT[ACGT]ACC
FauI          287  GCGGG                CCCGC(4/6)
HindIII        90  AAGCTT               A^AGCTT
HpaI         1027  GTTAAC               GTT^AAC
MamI          133  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          368  CAATTG               C^AATTG
MluNI         168  TGGCCA               TGG^CCA
MroI          572  TCCGGA               T^CCGGA
MscI          168  TGGCCA               TGG^CCA
MseI         1028  TTAA                 T^TAA
MunI          368  CAATTG               C^AATTG
NciI           10  CCCGG                CC^[GC]GG
NdeI          686  CATATG               CA^TATG
NspI          248  ACATGC               [GA]CATG^[CT]
NspV         1439  TTCGAA               TT^CGAA
SfuI         1439  TTCGAA               TT^CGAA
SmlI         1145  CTCAAG               C^T[CT][GA]AG
SpeI          538  ACTAGT               A^CTAGT
Tru9I        1028  TTAA                 T^TAA
XbaI         1831  TCTAGA               T^CTAGA
XcmI         1059  CCAGGCATCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          287  GCGG                 CCGC(-3/-1)
             1551  GCGG                
AcsI          106  GAATTT               [GA]^AATT[CT]
             1836  AAATTC              
AfaI          376  GTAC                 GT^AC
             1217  GTAC                
AlwNI        1280  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1283  CAGCTGCTG           
AocI         1570  CCTGAGG              CC^T[ACGT]AGG
             1636  CCTGAGG             
ApoI          106  GAATTT               [GA]^AATT[CT]
             1836  AAATTC              
AvaII         234  GGTCC                G^G[AT]CC
              324  GGACC               
BanI          478  GGCACC               G^G[CT][GA]CC
             1590  GGCACC              
BclI          432  TGATCA               T^GATCA
             1619  TGATCA              
BstYI         654  AGATCC               [GA]^GATC[CT]
              915  GGATCC              
Bsu36I       1570  CCTGAGG              CC^T[ACGT]AGG
             1636  CCTGAGG             
DraII          42  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              629  AGGCCCC             
DsaI          267  CCATGG               C^C[GA][CT]GG
              314  CCATGG              
Eco81I       1570  CCTGAGG              CC^T[ACGT]AGG
             1636  CCTGAGG             
EcoO109I       42  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              629  AGGCCCC             
FbaI          432  TGATCA               T^GATCA
             1619  TGATCA              
HapII          11  CCGG                 C^CGG
              573  CCGG                
HpaII          11  CCGG                 C^CGG
              573  CCGG                
MflI          654  AGATCC               [GA]^GATC[CT]
              915  GGATCC              
MspA1I        454  CAGCTG               C[AC]G^C[GT]G
             1283  CAGCTG              
MspI           11  CCGG                 C^CGG
              573  CCGG                
NcoI          267  CCATGG               C^CATGG
              314  CCATGG              
PflMI         237  CCAGACATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1578  CCATACAGTGG         
PleI         1365  GACTC                GAGTC(4/5)
             1645  GAGTC               
PvuII         454  CAGCTG               CAG^CTG
             1283  CAGCTG              
RsaI          376  GTAC                 GT^AC
             1217  GTAC                
TaqI         1440  TCGA                 T^CGA
             1710  TCGA                
TthHB8I      1440  TCGA                 T^CGA
             1710  TCGA                
Van91I        237  CCAGACATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1578  CCATACAGTGG         
XhoII         654  AGATCC               [GA]^GATC[CT]
              915  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AluI        AlwI        BanII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       
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