Restriction map for pF1KA0737
 (Linear) (Six-base) MAPSORT of: KIAA0737.seq  Check: 3054  from: 1  to: 1863



KIAA0737.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     501    1863 
   Size:        501    1362
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     871    1863 
   Size:        527     344     992

  Fragments arranged by size: 

                 992     527     344
 
AleI CACnn'nnGTG

Cuts at:      0    1180    1849    1863 
   Size:       1180     669      14

  Fragments arranged by size: 

                1180     669      14
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     502    1863 
   Size:        502    1361
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1285    1288    1863 
   Size:       1285       3     575

  Fragments arranged by size: 

                1285     575       3
 
ApoI r'AATT_y

Cuts at:      0     106    1836    1863 
   Size:        106    1730      27

  Fragments arranged by size: 

                1730     106      27
 
AvaI C'yCGr_G

Cuts at:      0    1190    1863 
   Size:       1190     673
 
AvrII C'CTAG_G

Cuts at:      0     468    1863 
   Size:        468    1395
 
BamHI G'GATC_C

Cuts at:      0     915    1863 
   Size:        915     948
 
BanI G'GyrC_C

Cuts at:      0     478    1590    1863 
   Size:        478    1112     273

  Fragments arranged by size: 

                1112     478     273
 
BanII G_rGCy'C

Cuts at:      0     296    1001    1199    1863 
   Size:        296     705     198     664

  Fragments arranged by size: 

                 705     664     296     198
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     601    1863 
   Size:        601    1262
 
BclI T'GATC_A

Cuts at:      0     432    1619    1863 
   Size:        432    1187     244

  Fragments arranged by size: 

                1187     432     244
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     219     492    1229    1767    1863 
   Size:        219     273     737     538      96

  Fragments arranged by size: 

                 737     538     273     219      96
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1505    1700    1863 
   Size:       1505     195     163

  Fragments arranged by size: 

                1505     195     163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962    1091    1390    1863 
   Size:        962     129     299     473

  Fragments arranged by size: 

                 962     473     299     129
 
Bpu10I CC'TnA_GC

Cuts at:      0    1547    1863 
   Size:       1547     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1130    1863 
   Size:       1130     733
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1688    1863 
   Size:       1688     175
 
BsaBI GATnn'nnATC

Cuts at:      0     137    1863 
   Size:        137    1726
 
BsaWI w'CCGG_w

Cuts at:      0     572    1863 
   Size:        572    1291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     927     957    1142    1172    1863 
   Size:        927      30     185      30     691

  Fragments arranged by size: 

                 927     691     185      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     811    1388    1454    1457    1863 
   Size:        811     577      66       3     406

  Fragments arranged by size: 

                 811     577     406      66       3
 
BseYI C'CCAG_C

Cuts at:      0     452     456     586     969    1075    1170    1703    1863 
   Size:        452       4     130     383     106      95     533     160

  Fragments arranged by size: 

                 533     452     383     160     130     106      95       4
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     296    1001    1199    1863 
   Size:        296     705     198     664

  Fragments arranged by size: 

                 705     664     296     198
 
BspEI T'CCGG_A

Cuts at:      0     572    1863 
   Size:        572    1291
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     501    1863 
   Size:        501    1362
 
BsrBI CCG'CTC

Cuts at:      0    1551    1863 
   Size:       1551     312
 
BsrDI GCAATG_nn'

Cuts at:      0    1785    1863 
   Size:       1785      78
 
BssSI C'ACGA_G

Cuts at:      0     550    1863 
   Size:        550    1313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     862    1285    1288    1863 
   Size:        862     423       3     575

  Fragments arranged by size: 

                 862     575     423       3
 
BstBI TT'CG_AA

Cuts at:      0    1440    1863 
   Size:       1440     423
 
BstEII G'GTnAC_C

Cuts at:      0    1182    1863 
   Size:       1182     681
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     321    1863 
   Size:        321    1542
 
BstYI r'GATC_y

Cuts at:      0     654     915    1863 
   Size:        654     261     948

  Fragments arranged by size: 

                 948     654     261
 
Bsu36I CC'TnA_GG

Cuts at:      0    1571    1637    1863 
   Size:       1571      66     226

  Fragments arranged by size: 

                1571     226      66
 
BtgI C'CryG_G

Cuts at:      0     267     314    1863 
   Size:        267      47    1549

  Fragments arranged by size: 

                1549     267      47
 
BtsI GCAGTG_nn'

Cuts at:      0    1797    1863 
   Size:       1797      66
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     749     784    1829    1863 
   Size:        749      35    1045      34

  Fragments arranged by size: 

                1045     749      35      34
 
EaeI y'GGCC_r

Cuts at:      0     168    1863 
   Size:        168    1695
 
EarI CTCTTCn'nnn_

Cuts at:      0     204     634    1134    1863 
   Size:        204     430     500     729

  Fragments arranged by size: 

                 729     500     430     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     871     962    1091    1390    1863 
   Size:        527     344      91     129     299     473

  Fragments arranged by size: 

                 527     473     344     299     129      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43     630    1863 
   Size:         43     587    1233

  Fragments arranged by size: 

                1233     587      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     532    1863 
   Size:        532    1331
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     379     411     958     990    1242    1274 
   Size:        181      32     166      32     547      32     252      32

Cuts at:   1274    1630    1662    1863 
   Size:        356      32     201

  Fragments arranged by size: 

                 547     356     252     201     181     166      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     422     966    1029    1240    1863 
   Size:        422     544      63     211     623

  Fragments arranged by size: 

                 623     544     422     211      63
 
HindIII A'AGCT_T

Cuts at:      0      90    1863 
   Size:         90    1773
 
HpaI GTT'AAC

Cuts at:      0    1029    1863 
   Size:       1029     834
 
MfeI C'AATT_G

Cuts at:      0     368    1863 
   Size:        368    1495
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1224    1863 
   Size:       1224     639
 
MscI TGG'CCA

Cuts at:      0     170    1863 
   Size:        170    1693
 
MslI CAynn'nnrTG

Cuts at:      0     176     253     554    1180    1849    1863 
   Size:        176      77     301     626     669      14

  Fragments arranged by size: 

                 669     626     301     176      77      14
 
MspA1I CmG'CkG

Cuts at:      0     456    1285    1863 
   Size:        456     829     578

  Fragments arranged by size: 

                 829     578     456
 
NcoI C'CATG_G

Cuts at:      0     267     314    1863 
   Size:        267      47    1549

  Fragments arranged by size: 

                1549     267      47
 
NdeI CA'TA_TG

Cuts at:      0     687    1863 
   Size:        687    1176
 
NspI r_CATG'y

Cuts at:      0     252    1863 
   Size:        252    1611
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243    1584    1863 
   Size:        243    1341     279

  Fragments arranged by size: 

                1341     279     243
 
PfoI T'CCnGG_A

Cuts at:      0       9    1863 
   Size:          9    1854
 
PstI C_TGCA'G

Cuts at:      0    1391    1475    1777    1804    1863 
   Size:       1391      84     302      27      59

  Fragments arranged by size: 

                1391     302      84      59      27
 
PvuII CAG'CTG

Cuts at:      0     456    1285    1863 
   Size:        456     829     578

  Fragments arranged by size: 

                 829     578     456
 
SfcI C'TryA_G

Cuts at:      0     332    1327    1387    1471    1773    1800    1863 
   Size:        332     995      60      84     302      27      63

  Fragments arranged by size: 

                 995     332     302      84      63      60      27
 
SmlI C'TyrA_G

Cuts at:      0    1145    1863 
   Size:       1145     718
 
SpeI A'CTAG_T

Cuts at:      0     538    1863 
   Size:        538    1325
 
StyI C'CwwG_G

Cuts at:      0     128     267     314     468    1863 
   Size:        128     139      47     154    1395

  Fragments arranged by size: 

                1395     154     139     128      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85    1250    1863 
   Size:         85    1165     613

  Fragments arranged by size: 

                1165     613      85
 
TatI w'GTAC_w

Cuts at:      0    1216    1863 
   Size:       1216     647
 
XbaI T'CTAG_A

Cuts at:      0    1831    1863 
   Size:       1831      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1066    1863 
   Size:       1066     797
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI     BclI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI
     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
    PflMI     PfoI     PstI    PvuII     SfcI     SmlI     SpeI     StyI
    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI    BsaAI    BsaHI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspHI    BsrFI    BsrGI   BssHII  BstZ17I
    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI
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