Restriction map for pF1KA0713
 (Linear) (Six-base) MAPSORT of: KIAA0713.seq  Check: 7221  from: 1  to: 2871



KIAA0713.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AccI GT'mk_AC

Cuts at:      0    1136    2871 
   Size:       1136    1735
 
AclI AA'CG_TT

Cuts at:      0    2505    2871 
   Size:       2505     366
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1491    2653    2871 
   Size:       1491    1162     218

  Fragments arranged by size: 

                1491    1162     218
 
AflIII A'CryG_T

Cuts at:      0    1550    2871 
   Size:       1550    1321
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1324    1956    2260    2548    2871 
   Size:       1324     632     304     288     323

  Fragments arranged by size: 

                1324     632     323     304     288
 
ApaLI G'TGCA_C

Cuts at:      0     639    2871 
   Size:        639    2232
 
ApoI r'AATT_y

Cuts at:      0     770    1331    1629    2770    2871 
   Size:        770     561     298    1141     101

  Fragments arranged by size: 

                1141     770     561     298     101
 
AseI AT'TA_AT

Cuts at:      0     784     815    2438    2871 
   Size:        784      31    1623     433

  Fragments arranged by size: 

                1623     784     433      31
 
BamHI G'GATC_C

Cuts at:      0    1576    2871 
   Size:       1576    1295
 
BanI G'GyrC_C

Cuts at:      0     286    2226    2871 
   Size:        286    1940     645

  Fragments arranged by size: 

                1940     645     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2721    2871 
   Size:       2721     150
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1216    2871 
   Size:       1216    1655
 
BclI T'GATC_A

Cuts at:      0     549    1716    2871 
   Size:        549    1167    1155

  Fragments arranged by size: 

                1167    1155     549
 
BfrBI ATG'CAT

Cuts at:      0    1735    1942    2518    2871 
   Size:       1735     207     576     353

  Fragments arranged by size: 

                1735     576     353     207
 
BglII A'GATC_T

Cuts at:      0     573     952    2871 
   Size:        573     379    1919

  Fragments arranged by size: 

                1919     573     379
 
BlpI GC'TnA_GC

Cuts at:      0    1541    2871 
   Size:       1541    1330
 
Bme1580I G_kGCm'C

Cuts at:      0     643    2871 
   Size:        643    2228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     734    1202    2871 
   Size:        295     439     468    1669

  Fragments arranged by size: 

                1669     468     439     295
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354    1020    1842    2038    2831    2871 
   Size:        354     666     822     196     793      40

  Fragments arranged by size: 

                 822     793     666     354     196      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1616    2871 
   Size:       1616    1255
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1792    2871 
   Size:       1792    1079
 
BseYI C'CCAG_C

Cuts at:      0    1986    2871 
   Size:       1986     885
 
BsiHKAI G_wGCw'C

Cuts at:      0     643    2871 
   Size:        643    2228
 
BsmI GAATG_Cn'

Cuts at:      0    1127    1521    2796    2871 
   Size:       1127     394    1275      75

  Fragments arranged by size: 

                1275    1127     394      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     643    2871 
   Size:        643    2228
 
BspHI T'CATG_A

Cuts at:      0    1419    2871 
   Size:       1419    1452
 
BsrFI r'CCGG_y

Cuts at:      0    1757    2871 
   Size:       1757    1114
 
BsrGI T'GTAC_A

Cuts at:      0    1752    2871 
   Size:       1752    1119
 
BssSI C'ACGA_G

Cuts at:      0     764    2871 
   Size:        764    2107
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1741    2871 
   Size:       1741    1130
 
BstBI TT'CG_AA

Cuts at:      0    2574    2871 
   Size:       2574     297
 
BstEII G'GTnAC_C

Cuts at:      0    1230    2871 
   Size:       1230    1641
 
BstYI r'GATC_y

Cuts at:      0     573     952    1425    1560    1576    1932    2871 
   Size:        573     379     473     135      16     356     939

  Fragments arranged by size: 

                 939     573     473     379     356     135      16
 
Bsu36I CC'TnA_GG

Cuts at:      0     670    2871 
   Size:        670    2201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     386    2251    2871 
   Size:        386    1865     620

  Fragments arranged by size: 

                1865     620     386
 
DraIII CAC_nnn'GTG

Cuts at:      0    2004    2871 
   Size:       2004     867
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2160    2871 
   Size:       2160     711
 
EaeI y'GGCC_r

Cuts at:      0    2524    2871 
   Size:       2524     347
 
EarI CTCTTCn'nnn_

Cuts at:      0     413    2871 
   Size:        413    2458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     734    1202    1491    2653    2871 
   Size:        295     439     468     289    1162     218

  Fragments arranged by size: 

                1162     468     439     295     289     218
 
EcoRI G'AATT_C

Cuts at:      0     770    2871 
   Size:        770    2101
 
EcoRV GAT'ATC

Cuts at:      0     978    2871 
   Size:        978    1893
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2689    2871 
   Size:       2689     182
 
FspI TGC'GCA

Cuts at:      0    1531    2871 
   Size:       1531    1340
 
FspAI rTGC'GCAy

Cuts at:      0    1531    2871 
   Size:       1531    1340
 
HaeII r_GCGC'y

Cuts at:      0    2854    2871 
   Size:       2854      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     364     396     731     763    1582    1614    2871 
   Size:        364      32     335      32     819      32    1257

  Fragments arranged by size: 

                1257     819     364     335      32      32      32
 
HincII GTy'rAC

Cuts at:      0       5    2454    2463    2871 
   Size:          5    2449       9     408

  Fragments arranged by size: 

                2449     408       9       5
 
HpaI GTT'AAC

Cuts at:      0       5    2871 
   Size:          5    2866
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     229    1308    1453    2871 
   Size:        229    1079     145    1418

  Fragments arranged by size: 

                1418    1079     229     145
 
MscI TGG'CCA

Cuts at:      0    2526    2871 
   Size:       2526     345
 
MslI CAynn'nnrTG

Cuts at:      0     832    2871 
   Size:        832    2039
 
MspA1I CmG'CkG

Cuts at:      0     967    1321    2871 
   Size:        967     354    1550

  Fragments arranged by size: 

                1550     967     354
 
NdeI CA'TA_TG

Cuts at:      0      25     829    1731    2424    2871 
   Size:         25     804     902     693     447

  Fragments arranged by size: 

                 902     804     693     447      25
 
NsiI A_TGCA'T

Cuts at:      0    1737    1944    2520    2871 
   Size:       1737     207     576     351

  Fragments arranged by size: 

                1737     576     351     207
 
NspI r_CATG'y

Cuts at:      0    1173    1536    1554    2871 
   Size:       1173     363      18    1317

  Fragments arranged by size: 

                1317    1173     363      18
 
PciI A'CATG_T

Cuts at:      0    1550    2871 
   Size:       1550    1321
 
PfoI T'CCnGG_A

Cuts at:      0     711    1875    2871 
   Size:        711    1164     996

  Fragments arranged by size: 

                1164     996     711
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2014    2046    2871 
   Size:       2014      32     825

  Fragments arranged by size: 

                2014     825      32
 
PvuII CAG'CTG

Cuts at:      0     967    1321    2871 
   Size:        967     354    1550

  Fragments arranged by size: 

                1550     967     354
 
SfcI C'TryA_G

Cuts at:      0    1616    2031    2871 
   Size:       1616     415     840

  Fragments arranged by size: 

                1616     840     415
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1757    2871 
   Size:       1757    1114
 
SmlI C'TyrA_G

Cuts at:      0     333    1035    1857    2017    2846    2871 
   Size:        333     702     822     160     829      25

  Fragments arranged by size: 

                 829     822     702     333     160      25
 
SspI AAT'ATT

Cuts at:      0      86     897    2310    2871 
   Size:         86     811    1413     561

  Fragments arranged by size: 

                1413     811     561      86
 
StuI AGG'CCT

Cuts at:      0     865    2871 
   Size:        865    2006
 
StyI C'CwwG_G

Cuts at:      0      42    2871 
   Size:         42    2829
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109    2871 
   Size:        109    2762
 
TatI w'GTAC_w

Cuts at:      0    1674    1752    2871 
   Size:       1674      78    1119

  Fragments arranged by size: 

                1674    1119      78
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     825     857    2871 
   Size:        825      32    2014

  Fragments arranged by size: 

                2014     825      32
 
XbaI T'CTAG_A

Cuts at:      0     957    2871 
   Size:        957    1914
 
XmnI GAAnn'nnTTC

Cuts at:      0     383    2506    2690    2871 
   Size:        383    2123     184     181

  Fragments arranged by size: 

                2123     383     184     181
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI     AseI
    BamHI     BanI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BpmI    BpuEI     BsaI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstYI   Bsu36I    BtgZI   DraIII     DrdI     EaeI     EarI  Eco57MI
    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI
     PciI     PfoI     PsrI    PvuII     SfcI    SgrAI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI
    BanII     BbeI    BbvCI     BcgI     BglI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI   BssHII
    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FseI  HindIII     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XcmI     XhoI     XmaI     ZraI
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