Restriction map for pF1KA0687
 (Linear) (Six-base) MAPSORT of: KIAA0687.seq  Check: 6301  from: 1  to: 3111



KIAA0687.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0     126    1048    2342    3111 
   Size:        126     922    1294     769

  Fragments arranged by size: 

                1294     922     769     126
 
AclI AA'CG_TT

Cuts at:      0    1095    3111 
   Size:       1095    2016
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778    1455    1517    1562    3111 
   Size:        778     677      62      45    1549

  Fragments arranged by size: 

                1549     778     677      62      45
 
AflII C'TTAA_G

Cuts at:      0     904    3111 
   Size:        904    2207
 
AflIII A'CryG_T

Cuts at:      0     129    2337    2703    3111 
   Size:        129    2208     366     408

  Fragments arranged by size: 

                2208     408     366     129
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2959    2991    3111 
   Size:       2959      32     120

  Fragments arranged by size: 

                2959     120      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1518    3111 
   Size:       1518    1593
 
ApoI r'AATT_y

Cuts at:      0    1776    2181    2473    2539    3004    3111 
   Size:       1776     405     292      66     465     107

  Fragments arranged by size: 

                1776     465     405     292     107      66
 
AvaI C'yCGr_G

Cuts at:      0    1180    1429    3111 
   Size:       1180     249    1682

  Fragments arranged by size: 

                1682    1180     249
 
BamHI G'GATC_C

Cuts at:      0      57    1420    2635    3111 
   Size:         57    1363    1215     476

  Fragments arranged by size: 

                1363    1215     476      57
 
BanI G'GyrC_C

Cuts at:      0     100     803    1374    2520    3111 
   Size:        100     703     571    1146     591

  Fragments arranged by size: 

                1146     703     591     571     100
 
BanII G_rGCy'C

Cuts at:      0     280    1187    1343    2994    3111 
   Size:        280     907     156    1651     117

  Fragments arranged by size: 

                1651     907     280     156     117
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     386    2268    3111 
   Size:        386    1882     843

  Fragments arranged by size: 

                1882     843     386
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      32    1857    1891    3111 
   Size:         32    1825      34    1220

  Fragments arranged by size: 

                1825    1220      34      32
 
BfrBI ATG'CAT

Cuts at:      0     708    3111 
   Size:        708    2403
 
BlpI GC'TnA_GC

Cuts at:      0     527    3111 
   Size:        527    2584
 
Bme1580I G_kGCm'C

Cuts at:      0    1379    1870    2523    3111 
   Size:       1379     491     653     588

  Fragments arranged by size: 

                1379     653     588     491
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1755    3111 
   Size:       1755    1356
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     266     384    1894    3111 
   Size:        223      43     118    1510    1217

  Fragments arranged by size: 

                1510    1217     223     118      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1555    2308    3111 
   Size:       1555     753     803

  Fragments arranged by size: 

                1555     803     753
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1439    1451    2022    3111 
   Size:       1439      12     571    1089

  Fragments arranged by size: 

                1439    1089     571      12
 
BsaAI yAC'GTr

Cuts at:      0    2338    3111 
   Size:       2338     773
 
BsaBI GATnn'nnATC

Cuts at:      0    1485    3111 
   Size:       1485    1626
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61    2555    2585    3111 
   Size:         31      30    2494      30     526

  Fragments arranged by size: 

                2494     526      31      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36     737    1003    1389    1653    1690    1711    1920 
   Size:         36     701     266     386     264      37      21     209

Cuts at:   1920    3111 
   Size:       1191

  Fragments arranged by size: 

                1191     701     386     266     264     209      37      36
                  21
 
BseYI C'CCAG_C

Cuts at:      0      64     328    1424    2918    3111 
   Size:         64     264    1096    1494     193

  Fragments arranged by size: 

                1494    1096     264     193      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     528     690     723    3111 
   Size:        528     162      33    2388

  Fragments arranged by size: 

                2388     528     162      33
 
BsmI GAATG_Cn'

Cuts at:      0     495    3111 
   Size:        495    2616
 
Bsp1286I G_dGCh'C

Cuts at:      0     280     528     690     723    1187    1343    1379    1870 
   Size:        280     248     162      33     464     156      36     491

Cuts at:   1870    2523    2994    3111 
   Size:        653     471     117

  Fragments arranged by size: 

                 653     491     471     464     280     248     162     156
                 117      36      33
 
BspHI T'CATG_A

Cuts at:      0    3077    3111 
   Size:       3077      34
 
BsrDI GCAATG_nn'

Cuts at:      0     242    1791    3027    3111 
   Size:        242    1549    1236      84

  Fragments arranged by size: 

                1549    1236     242      84
 
BsrFI r'CCGG_y

Cuts at:      0    1842    3111 
   Size:       1842    1269
 
BsrGI T'GTAC_A

Cuts at:      0    2439    3111 
   Size:       2439     672
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     685    3111 
   Size:        685    2426
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     675    3111 
   Size:        675    2436
 
BstYI r'GATC_y

Cuts at:      0      57    1420    1590    2049    2592    2635    2939    3111 
   Size:         57    1363     170     459     543      43     304     172

  Fragments arranged by size: 

                1363     543     459     304     172     170      57      43
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     412     590    1401    1740    3084    3111 
   Size:        412     178     811     339    1344      27

  Fragments arranged by size: 

                1344     811     412     339     178      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     582    3111 
   Size:        582    2529
 
BtsI GCAGTG_nn'

Cuts at:      0    1276    1293    1395    1535    1628    3111 
   Size:       1276      17     102     140      93    1483

  Fragments arranged by size: 

                1483    1276     140     102      93      17
 
DraIII CAC_nnn'GTG

Cuts at:      0    2107    3111 
   Size:       2107    1004
 
EaeI y'GGCC_r

Cuts at:      0    1604    1636    1844    2229    3111 
   Size:       1604      32     208     385     882

  Fragments arranged by size: 

                1604     882     385     208      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     928     980    1058    1112    2131    2488    3104 
   Size:        179     749      52      78      54    1019     357     616

Cuts at:   3104    3111 
   Size:          7

  Fragments arranged by size: 

                1019     749     616     357     179      78      54      52
                   7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     564    1870    3111 
   Size:        564    1306    1241

  Fragments arranged by size: 

                1306    1241     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     266     384     778    1455    1517    1562    1894 
   Size:        223      43     118     394     677      62      45     332

Cuts at:   1894    3111 
   Size:       1217

  Fragments arranged by size: 

                1217     677     394     332     223     118      62      45
                  43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3087    3111 
   Size:       3087      24
 
EcoRV GAT'ATC

Cuts at:      0     459    3111 
   Size:        459    2652
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1185    3051    3111 
   Size:       1185    1866      60

  Fragments arranged by size: 

                1866    1185      60
 
HaeII r_GCGC'y

Cuts at:      0    1518    3111 
   Size:       1518    1593
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63     634     666    1648    1680    2659    2691 
   Size:         31      32     571      32     982      32     979      32

Cuts at:   2691    2929    2961    3111 
   Size:        238      32     150

  Fragments arranged by size: 

                 982     979     571     238     150      32      32      32
                  32      32      31
 
HincII GTy'rAC

Cuts at:      0     127     510    3111 
   Size:        127     383    2601

  Fragments arranged by size: 

                2601     383     127
 
HindIII A'AGCT_T

Cuts at:      0    1167    3111 
   Size:       1167    1944
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      72    3111 
   Size:         72    3039
 
MscI TGG'CCA

Cuts at:      0    1606    2231    3111 
   Size:       1606     625     880

  Fragments arranged by size: 

                1606     880     625
 
MslI CAynn'nnrTG

Cuts at:      0     673    1826    2966    3111 
   Size:        673    1153    1140     145

  Fragments arranged by size: 

                1153    1140     673     145
 
MspA1I CmG'CkG

Cuts at:      0     531     828    3111 
   Size:        531     297    2283

  Fragments arranged by size: 

                2283     531     297
 
NaeI GCC'GGC

Cuts at:      0    1844    3111 
   Size:       1844    1267
 
NdeI CA'TA_TG

Cuts at:      0    2832    3111 
   Size:       2832     279
 
NgoMIV G'CCGG_C

Cuts at:      0    1842    3111 
   Size:       1842    1269
 
NsiI A_TGCA'T

Cuts at:      0     710    3111 
   Size:        710    2401
 
NspI r_CATG'y

Cuts at:      0     133     708     825    2707    3111 
   Size:        133     575     117    1882     404

  Fragments arranged by size: 

                1882     575     404     133     117
 
PasI CC'CwG_GG

Cuts at:      0    1441    3111 
   Size:       1441    1670
 
PciI A'CATG_T

Cuts at:      0     129    2703    3111 
   Size:        129    2574     408

  Fragments arranged by size: 

                2574     408     129
 
PfoI T'CCnGG_A

Cuts at:      0     282    1142    3111 
   Size:        282     860    1969

  Fragments arranged by size: 

                1969     860     282
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1227    1259    2707    2739    3111 
   Size:       1227      32    1448      32     372

  Fragments arranged by size: 

                1448    1227     372      32      32
 
PsiI TTA'TAA

Cuts at:      0     865    2447    3111 
   Size:        865    1582     664

  Fragments arranged by size: 

                1582     865     664
 
PstI C_TGCA'G

Cuts at:      0    1079    1283    3111 
   Size:       1079     204    1828

  Fragments arranged by size: 

                1828    1079     204
 
PvuII CAG'CTG

Cuts at:      0     531    3111 
   Size:        531    2580
 
SalI G'TCGA_C

Cuts at:      0     125    3111 
   Size:        125    2986
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1112    3111 
   Size:       1112    1999
 
SfcI C'TryA_G

Cuts at:      0     836    1075    1126    1279    1963    2860    3111 
   Size:        836     239      51     153     684     897     251

  Fragments arranged by size: 

                 897     836     684     251     239     153      51
 
SmaI CCC'GGG

Cuts at:      0    1431    3111 
   Size:       1431    1680
 
SmlI C'TyrA_G

Cuts at:      0     904    1570    2287    3111 
   Size:        904     666     717     824

  Fragments arranged by size: 

                 904     824     717     666
 
StyI C'CwwG_G

Cuts at:      0    1859    2232    2846    3111 
   Size:       1859     373     614     265

  Fragments arranged by size: 

                1859     614     373     265
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1226    3038    3111 
   Size:       1226    1812      73

  Fragments arranged by size: 

                1812    1226      73
 
TatI w'GTAC_w

Cuts at:      0     965    2439    3111 
   Size:        965    1474     672

  Fragments arranged by size: 

                1474     965     672
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1909    1941    1976    2008    3111 
   Size:       1909      32      35      32    1103

  Fragments arranged by size: 

                1909    1103      35      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1890    3111 
   Size:       1890    1221
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162    2910    3111 
   Size:        162    2748     201

  Fragments arranged by size: 

                2748     201     162
 
XmaI C'CCGG_G

Cuts at:      0    1429    3111 
   Size:       1429    1682
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AloI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BfrBI     BlpI
 Bme1580I     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I    BtgZI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI
     NdeI   NgoMIV     NsiI     NspI     PasI     PciI     PfoI     PpiI
     PsiI     PstI    PvuII     SalI     SapI     SfcI     SmaI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AhdI     AleI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI    BciVI     BclI     BglI    BglII    BmgBI     BmrI     BmtI
   Bpu10I    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtgI
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI EcoO109I    EcoRI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NheI     NotI     NruI     PacI    PflMI     PmeI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmnI     ZraI
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