Restriction map for pF1KA0660
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
ClaI        CpoI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII         78  TCCGGA               T^CCGGA
AclI         1066  AACGTT               AA^CGTT
AlwI          352  GGATC                GGATC(4/5)
Asp700I      1147  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1436  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          168  TGGCCA               TGG^CCA
BbrPI        1310  CACGTG               CAC^GTG
BsaAI        1310  CACGTG               [CT]AC^GT[GA]
BsaBI         985  GATAGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          78  TCCGGA               [AT]^CCGG[AT]
BseAI          78  TCCGGA               T^CCGGA
BsmAI         817  GTCTC                GTCTC(1/5)
BsmFI        1298  GTCCC                GGGAC(10/14)
BspEI          78  TCCGGA               T^CCGGA
BspMI         758  ACCTGC               ACCTGC(4/8)
BsrDI        1286  GCAATG               GCAATG(2/0)
BssSI         866  CTCGTG               CACGAG(-5/-1)
BstF5I        240  GGATG                GGATG(2/0)
BstYI         352  GGATCT               [GA]^GATC[CT]
CfoI         1284  GCGC                 GCG^C
DraI         1203  TTTAAA               TTT^AAA
EaeI          168  TGGCCA               [CT]^GGCC[GA]
EcoNI         907  CCTCCTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI           36  GCGGG                CCCGC(4/6)
FokI          240  GGATG                GGATG(9/13)
HgaI         1440  GCGTC                GACGC(5/10)
HhaI         1284  GCGC                 GCG^C
HinP1I       1284  GCGC                 G^CGC
HindIII      1108  AAGCTT               A^AGCTT
MamI          985  GATAGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          352  GGATCT               [GA]^GATC[CT]
MluNI         168  TGGCCA               TGG^CCA
MroI           78  TCCGGA               T^CCGGA
MscI          168  TGGCCA               TGG^CCA
MslI          235  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         22  CCGCTG               C[AC]G^C[GT]G
PflFI        1436  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1310  CACGTG               CAC^GTG
PmlI         1310  CACGTG               CAC^GTG
Psp1406I     1066  AACGTT               AA^CGTT
SapI          619  GAAGAGC              GCTCTTC(1/4)
SexAI         897  ACCTGGT              A^CC[AT]GGT
SmlI          255  CTTGAG               C^T[CT][GA]AG
Tth111I      1436  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         352  GGATCT               [GA]^GATC[CT]
XmnI         1147  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          773  GTAC                 GT^AC
             1198  GTAC                
AlwNI         599  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              692  CAGAACCTG           
BbvI           24  GCTGC                GCAGC(8/12)
             1231  GCTGC               
BcnI         1300  CCCGG                CC^[GC]GG
             1409  CCGGG               
BspHI         579  TCATGA               T^CATGA
             1052  TCATGA              
DraII        1296  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1356  AGGACCT             
DraIII        535  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              863  CACCTCGTG           
EcoO109I     1296  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1356  AGGACCT             
EcoT14I      1091  CCAAGG               C^C[AT][AT]GG
             1366  CCAAGG              
HaeIII        169  GGCC                 GG^CC
             1423  GGCC                
MwoI           15  GCCCAGTCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               27  GCTTGTAGGGC         
NciI         1300  CCCGG                CC^[GC]GG
             1409  CCGGG               
PpuMI        1296  AGGTCCC              [GA]G^G[AT]CC[CT]
             1356  AGGACCT             
RcaI          579  TCATGA               T^CATGA
             1052  TCATGA              
RsaI          773  GTAC                 GT^AC
             1198  GTAC                
SspI           53  AATATT               AAT^ATT
               83  AATATT              
StyI         1091  CCAAGG               C^C[AT][AT]GG
             1366  CCAAGG              
TseI           24  GCTGC                G^C[AT]GC
             1231  GCTGC               
Tsp45I        541  GTGAC                ^GT[GC]AC
              745  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AvaII       
BfaI        BsaJI       BseRI       BsiYI       BslI        
BsrI        BssKI       BstNI       Cfr13I      DdeI        
DpnII       EarI        EcoRI       EcoRII      Fnu4HI      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       Tsp509I     TspRI       TthHB8I     
Van91I      
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